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Protein

Myristoylated alanine-rich C-kinase substrate

Gene

Marcks

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

GO - Molecular functioni

  • actin filament binding Source: CAFA
  • calmodulin binding Source: CAFA
  • identical protein binding Source: CAFA
  • protein kinase C binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-399997 Acetylcholine regulates insulin secretion

Names & Taxonomyi

Protein namesi
Recommended name:
Myristoylated alanine-rich C-kinase substrate
Short name:
MARCKS
Gene namesi
Name:Marcks
Synonyms:Macs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:96907 Marcks

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi113S → A: Poorly phosphorylated. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001571492 – 309Myristoylated alanine-rich C-kinase substrateAdd BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei15PhosphothreonineCombined sources1
Modified residuei26PhosphoserineBy similarity1
Modified residuei27PhosphoserineCombined sources1
Modified residuei29PhosphoserineBy similarity1
Modified residuei46PhosphoserineCombined sources1
Modified residuei63PhosphoserineBy similarity1
Modified residuei74PhosphoserineBy similarity1
Modified residuei79PhosphothreonineBy similarity1
Modified residuei113Phosphoserine; by MAPK1 Publication1
Modified residuei122PhosphoserineBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei138PhosphoserineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Modified residuei143PhosphothreonineCombined sources1
Modified residuei152Phosphoserine; by PKC1 Publication1
Modified residuei156Phosphoserine; by PKC1 Publication1
Modified residuei160PhosphoserineBy similarity1
Modified residuei163Phosphoserine; by PKCCombined sources1 Publication1
Modified residuei171PhosphoserineCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei291PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.2 Publications

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP26645
MaxQBiP26645
PaxDbiP26645
PeptideAtlasiP26645
PRIDEiP26645
TopDownProteomicsiP26645

PTM databases

iPTMnetiP26645
PhosphoSitePlusiP26645
SwissPalmiP26645

Expressioni

Tissue specificityi

Brain, spleen, less in kidney and heart, and very low levels in liver.

Inductioni

By lipopolysaccharides (LPS).

Gene expression databases

BgeeiENSMUSG00000069662 Expressed in 297 organ(s), highest expression level in pineal body
GenevisibleiP26645 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CALM3P621582EBI-911805,EBI-397435From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201268, 9 interactors
IntActiP26645, 2 interactors
MINTiP26645
STRINGi10090.ENSMUSP00000090245

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00253
ProteinModelPortaliP26645
SMRiP26645
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26645

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 169Calmodulin-binding (PSD)Add BLAST25

Sequence similaritiesi

Belongs to the MARCKS family.Curated

Phylogenomic databases

eggNOGiENOG410J04K Eukaryota
ENOG4111SZ0 LUCA
GeneTreeiENSGT00730000111419
HOGENOMiHOG000113482
InParanoidiP26645
KOiK12561
OMAiARVGDFF
OrthoDBiEOG091G1B6O
TreeFamiTF332815

Family and domain databases

InterProiView protein in InterPro
IPR002101 MARCKS
PANTHERiPTHR14353 PTHR14353, 1 hit
PfamiView protein in Pfam
PF02063 MARCKS, 1 hit
PRINTSiPR00963 MARCKS
PROSITEiView protein in PROSITE
PS00826 MARCKS_1, 1 hit
PS00827 MARCKS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26645-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGAQFSKTAA KGEATAERPG EAAVASSPSK ANGQENGHVK VNGDASPAAA
60 70 80 90 100
EPGAKEELQA NGSAPAADKE EPASGSAATP AAAEKDEAAA ATEPGAGAAD
110 120 130 140 150
KEAAEAEPAE PSSPAAEAEG ASASSTSSPK AEDGAAPSPS SETPKKKKKR
160 170 180 190 200
FSFKKSFKLS GFSFKKSKKE SGEGAEAEGA TAEGAKDEAA AAAGGEGAAA
210 220 230 240 250
PGEQAGGAGA EGAAGGEPRE AEAAEPEQPE QPEQPAAEEP QAEEQSEAAG
260 270 280 290 300
EKAEEPAPGA TAGDASSAAG PEQEAPAATD EAAASAAPAA SPEPQPECSP

EAPPAPTAE
Length:309
Mass (Da):29,661
Last modified:January 23, 2007 - v2
Checksum:i3BB1E392D74B5A98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96 – 98AGA → TGT AA sequence (PubMed:1868832).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60474 mRNA Translation: AAA39491.1
BC046601 mRNA Translation: AAH46601.1
CCDSiCCDS23784.1
PIRiA39169
RefSeqiNP_032564.1, NM_008538.2
UniGeneiMm.30059

Genome annotation databases

EnsembliENSMUST00000092584; ENSMUSP00000090245; ENSMUSG00000069662
GeneIDi17118
KEGGimmu:17118
UCSCiuc007evg.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60474 mRNA Translation: AAA39491.1
BC046601 mRNA Translation: AAH46601.1
CCDSiCCDS23784.1
PIRiA39169
RefSeqiNP_032564.1, NM_008538.2
UniGeneiMm.30059

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWQX-ray2.00B148-166[»]
DisProtiDP00253
ProteinModelPortaliP26645
SMRiP26645
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201268, 9 interactors
IntActiP26645, 2 interactors
MINTiP26645
STRINGi10090.ENSMUSP00000090245

PTM databases

iPTMnetiP26645
PhosphoSitePlusiP26645
SwissPalmiP26645

Proteomic databases

EPDiP26645
MaxQBiP26645
PaxDbiP26645
PeptideAtlasiP26645
PRIDEiP26645
TopDownProteomicsiP26645

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092584; ENSMUSP00000090245; ENSMUSG00000069662
GeneIDi17118
KEGGimmu:17118
UCSCiuc007evg.1 mouse

Organism-specific databases

CTDi4082
MGIiMGI:96907 Marcks

Phylogenomic databases

eggNOGiENOG410J04K Eukaryota
ENOG4111SZ0 LUCA
GeneTreeiENSGT00730000111419
HOGENOMiHOG000113482
InParanoidiP26645
KOiK12561
OMAiARVGDFF
OrthoDBiEOG091G1B6O
TreeFamiTF332815

Enzyme and pathway databases

ReactomeiR-MMU-399997 Acetylcholine regulates insulin secretion

Miscellaneous databases

ChiTaRSiMarcks mouse
EvolutionaryTraceiP26645
PROiPR:P26645
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000069662 Expressed in 297 organ(s), highest expression level in pineal body
GenevisibleiP26645 MM

Family and domain databases

InterProiView protein in InterPro
IPR002101 MARCKS
PANTHERiPTHR14353 PTHR14353, 1 hit
PfamiView protein in Pfam
PF02063 MARCKS, 1 hit
PRINTSiPR00963 MARCKS
PROSITEiView protein in PROSITE
PS00826 MARCKS_1, 1 hit
PS00827 MARCKS_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMARCS_MOUSE
AccessioniPrimary (citable) accession number: P26645
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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