UniProtKB - P26638 (SYSC_MOUSE)
Protein
Serine--tRNA ligase, cytoplasmic
Gene
Sars1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.By similarity
Catalytic activityi
: selenocysteinyl-tRNA(Sec) biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).Proteins known to be involved in this subpathway in this organism are:
- Seryl-tRNA synthetase (Sars), Serine--tRNA ligase, mitochondrial (Sars2), Serine--tRNA ligase, cytoplasmic (Sars1), Seryl-tRNA synthetase (Sars), Seryl-tRNA synthetase (Sars), Seryl-tRNA synthetase (Sars)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 271 | L-serineBy similarity | 1 | |
Binding sitei | 302 | L-serineBy similarity | 1 | |
Binding sitei | 325 | L-serineBy similarity | 1 | |
Binding sitei | 427 | L-serineBy similarity | 1 | |
Binding sitei | 429 | Important for serine bindingBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 302 – 304 | ATPBy similarity | 3 | |
Nucleotide bindingi | 318 – 321 | ATPBy similarity | 4 | |
Nucleotide bindingi | 391 – 394 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- enzyme binding Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- serine-tRNA ligase activity Source: UniProtKB
- tRNA binding Source: GO_Central
GO - Biological processi
- negative regulation of angiogenesis Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- negative regulation of vascular endothelial growth factor production Source: UniProtKB
- selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
- seryl-tRNA aminoacylation Source: UniProtKB
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, DNA-binding, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00906;UER00895 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11By similarity)Alternative name(s): Seryl-tRNA synthetase Short name: SerRS Seryl-tRNA(Ser/Sec) synthetase |
Gene namesi | Name:Sars1 Synonyms:Sars, Sers |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102809, Sars |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122192 | 1 – 512 | Serine--tRNA ligase, cytoplasmicAdd BLAST | 512 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 241 | PhosphoserineBy similarity | 1 | |
Modified residuei | 323 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 506 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P26638 |
jPOSTi | P26638 |
MaxQBi | P26638 |
PaxDbi | P26638 |
PeptideAtlasi | P26638 |
PRIDEi | P26638 |
2D gel databases
REPRODUCTION-2DPAGEi | P26638 |
PTM databases
iPTMneti | P26638 |
PhosphoSitePlusi | P26638 |
SwissPalmi | P26638 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000068739, Expressed in seminal vesicle and 305 other tissues |
Genevisiblei | P26638, MM |
Interactioni
Subunit structurei
Homodimer. The tRNA molecule may bind across the dimer.
Interacts with SIRT2.
Interacts with METTL6.
By similarityGO - Molecular functioni
- enzyme binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 203074, 28 interactors |
IntActi | P26638, 9 interactors |
MINTi | P26638 |
STRINGi | 10090.ENSMUSP00000099685 |
Miscellaneous databases
RNActi | P26638, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 61 | Interaction with tRNABy similarityAdd BLAST | 53 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 482 – 494 | Nuclear localization signalBy similarityAdd BLAST | 13 |
Domaini
Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG2509, Eukaryota |
GeneTreei | ENSGT00940000153792 |
InParanoidi | P26638 |
Family and domain databases
CDDi | cd00770, SerRS_core, 1 hit |
Gene3Di | 1.10.287.40, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR002317, Ser-tRNA-ligase_type_1 IPR015866, Ser-tRNA-synth_1_N IPR042103, SerRS_1_N_sf IPR033729, SerRS_core IPR010978, tRNA-bd_arm |
PANTHERi | PTHR11778, PTHR11778, 1 hit |
Pfami | View protein in Pfam PF02403, Seryl_tRNA_N, 1 hit PF00587, tRNA-synt_2b, 1 hit |
PIRSFi | PIRSF001529, Ser-tRNA-synth_IIa, 1 hit |
PRINTSi | PR00981, TRNASYNTHSER |
SUPFAMi | SSF46589, SSF46589, 1 hit |
TIGRFAMsi | TIGR00414, serS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P26638-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLDLDLFRV DKGGDPALIR ETQEKRFKDP GLVDQLVKAD SEWRRCRFRA
60 70 80 90 100
DNLNKLKNLC SKTIGEKMKK KEAVGDDESV PENVLNFDDL TADALAALKV
110 120 130 140 150
SQIKKVRLLI DEAIQKCDGE RVKLEAERFE NLREIGNLLH PSVPISNDED
160 170 180 190 200
ADNKVERIWG DCTVRKKYSH VDLVVMVDGF EGEKGAVVAG SRGYFLKGPL
210 220 230 240 250
VFLEQALIQY ALRTLGSRGY TPIYTPFFMR KEVMQEVAQL SQFDEELYKV
260 270 280 290 300
IGKGSEKSDD NSYDEKYLIA TSEQPIAALH RDEWLRPEDL PIKYAGLSTC
310 320 330 340 350
FRQEVGSHGR DTRGIFRVHQ FEKIEQFVYS SPHDNKSWEM FDEMIATAEE
360 370 380 390 400
FYQSLGIPYH IVNIVSGSLN HAASKKLDLE AWFPGSGAFR ELVSCSNCTD
410 420 430 440 450
YQARRLRIRY GQTKKMMDKV EFVHMLNATM CATTRTICAI LENYQAEKGI
460 470 480 490 500
AVPEKLREFM PPGLQELIPF VKPAPIDQEP SKKQKKQHEG SKKKAKEVPL
510
ENQLQSMEVT EA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8C483 | Q8C483_MOUSE | Seryl-tRNA synthetase | Sars Sars1 | 536 | Annotation score: | ||
A2AFS0 | A2AFS0_MOUSE | Seryl-tRNA synthetase | Sars | 352 | Annotation score: | ||
A2AFS1 | A2AFS1_MOUSE | Seryl-aminoacyl-tRNA synthetase | Sars | 186 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | A → R in AAA40108 (PubMed:1918033).Curated | 1 | |
Sequence conflicti | 192 – 199 | RGYFLKGP → PGVLPEGA in AAA40108 (PubMed:1918033).Curated | 8 | |
Sequence conflicti | 283 | E → A in AAA40108 (PubMed:1918033).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK075827 mRNA Translation: BAC35990.1 AL672200 Genomic DNA No translation available. BC008612 mRNA Translation: AAH08612.1 M74012 mRNA Translation: AAA40108.1 |
CCDSi | CCDS57254.1 |
RefSeqi | NP_001191908.1, NM_001204979.1 |
Genome annotation databases
Ensembli | ENSMUST00000090553; ENSMUSP00000088041; ENSMUSG00000068739 |
GeneIDi | 20226 |
KEGGi | mmu:20226 |
UCSCi | uc008qzb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK075827 mRNA Translation: BAC35990.1 AL672200 Genomic DNA No translation available. BC008612 mRNA Translation: AAH08612.1 M74012 mRNA Translation: AAA40108.1 |
CCDSi | CCDS57254.1 |
RefSeqi | NP_001191908.1, NM_001204979.1 |
3D structure databases
SMRi | P26638 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203074, 28 interactors |
IntActi | P26638, 9 interactors |
MINTi | P26638 |
STRINGi | 10090.ENSMUSP00000099685 |
PTM databases
iPTMneti | P26638 |
PhosphoSitePlusi | P26638 |
SwissPalmi | P26638 |
2D gel databases
REPRODUCTION-2DPAGEi | P26638 |
Proteomic databases
EPDi | P26638 |
jPOSTi | P26638 |
MaxQBi | P26638 |
PaxDbi | P26638 |
PeptideAtlasi | P26638 |
PRIDEi | P26638 |
Protocols and materials databases
Antibodypediai | 1608, 228 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000090553; ENSMUSP00000088041; ENSMUSG00000068739 |
GeneIDi | 20226 |
KEGGi | mmu:20226 |
UCSCi | uc008qzb.2, mouse |
Organism-specific databases
CTDi | 20226 |
MGIi | MGI:102809, Sars |
Phylogenomic databases
eggNOGi | KOG2509, Eukaryota |
GeneTreei | ENSGT00940000153792 |
InParanoidi | P26638 |
Enzyme and pathway databases
UniPathwayi | UPA00906;UER00895 |
Miscellaneous databases
BioGRID-ORCSi | 20226, 9 hits in 18 CRISPR screens |
ChiTaRSi | Sars, mouse |
PROi | PR:P26638 |
RNActi | P26638, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000068739, Expressed in seminal vesicle and 305 other tissues |
Genevisiblei | P26638, MM |
Family and domain databases
CDDi | cd00770, SerRS_core, 1 hit |
Gene3Di | 1.10.287.40, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR002317, Ser-tRNA-ligase_type_1 IPR015866, Ser-tRNA-synth_1_N IPR042103, SerRS_1_N_sf IPR033729, SerRS_core IPR010978, tRNA-bd_arm |
PANTHERi | PTHR11778, PTHR11778, 1 hit |
Pfami | View protein in Pfam PF02403, Seryl_tRNA_N, 1 hit PF00587, tRNA-synt_2b, 1 hit |
PIRSFi | PIRSF001529, Ser-tRNA-synth_IIa, 1 hit |
PRINTSi | PR00981, TRNASYNTHSER |
SUPFAMi | SSF46589, SSF46589, 1 hit |
TIGRFAMsi | TIGR00414, serS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYSC_MOUSE | |
Accessioni | P26638Primary (citable) accession number: P26638 Secondary accession number(s): A2AFR8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries