UniProtKB - P26612 (AMY2_ECOLI)
Protein
Cytoplasmic alpha-amylase
Gene
amyA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication EC:3.2.1.1
Cofactori
Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 104 | CalciumBy similarity | 1 | |
Metal bindingi | 198 | CalciumBy similarity | 1 | |
Active sitei | 235 | NucleophileBy similarity | 1 | |
Metal bindingi | 239 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 265 | Proton donorBy similarity | 1 | |
Sitei | 332 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- alpha-amylase activity Source: EcoCyc
- alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
- calcium ion binding Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Calcium, Metal-binding, Sodium |
Enzyme and pathway databases
BioCyci | EcoCyc:ALPHA-AMYL-CYTO-MONOMER MetaCyc:ALPHA-AMYL-CYTO-MONOMER |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytoplasmic alpha-amylase (EC:3.2.1.11 Publication)Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase |
Gene namesi | Name:amyA Synonyms:yedC Ordered Locus Names:b1927, JW1912 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication
GO - Cellular componenti
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054287 | 1 – 495 | Cytoplasmic alpha-amylaseAdd BLAST | 495 |
Proteomic databases
jPOSTi | P26612 |
PaxDbi | P26612 |
PRIDEi | P26612 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 4260380, 11 interactors |
DIPi | DIP-9108N |
IntActi | P26612, 4 interactors |
STRINGi | 511145.b1927 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 13 family.Curated
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
HOGENOMi | CLU_024572_2_0_6 |
InParanoidi | P26612 |
PhylomeDBi | P26612 |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR013776, A-amylase_thermo IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PIRSFi | PIRSF001021, Alph-amls_thrmst, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
i Sequence
Sequence statusi: Complete.
P26612-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG
60 70 80 90 100
GYSVGYDSYD LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD
110 120 130 140 150
VVVNHKMGAD EKEAIRVQRV NADDRTQIDE EIIECEGWTR YTFPARAGQY
160 170 180 190 200
SQFIWDFKCF SGIDHIENPD EDGIFKIVND YTGEGWNDQV DDELGNFDYL
210 220 230 240 250
MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI PAWFYKEWIE
260 270 280 290 300
HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS
310 320 330 340 350
RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL
360 370 380 390 400
AYALILLREN GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL
410 420 430 440 450
ARQRFAHGVQ TLFFDHPNCI AFSRSGTDEF PGCVVVMSNG DDGEKTIHLG
460 470 480 490
ENYGNKTWRD FLGNRQERVV TDENGEATFF CNGGSVSVWV IEEVI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 – 20 | KL → SS in AAA23810 (PubMed:1400215).Curated | 2 | |
Sequence conflicti | 109 | A → V in AAA23810 (PubMed:1400215).Curated | 1 | |
Sequence conflicti | 149 | Q → E in AAA23810 (PubMed:1400215).Curated | 1 | |
Sequence conflicti | 234 | L → I in AAA23810 (PubMed:1400215).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L01642 Genomic DNA Translation: AAA23810.1 U00096 Genomic DNA Translation: AAC74994.1 AP009048 Genomic DNA Translation: BAA15755.1 M85240 Genomic DNA No translation available. L13279 Genomic DNA Translation: AAA82575.1 |
PIRi | D64956, A45738 |
RefSeqi | NP_416437.1, NC_000913.3 WP_001245695.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74994; AAC74994; b1927 BAA15755; BAA15755; BAA15755 |
GeneIDi | 946434 |
KEGGi | ecj:JW1912 eco:b1927 |
PATRICi | fig|1411691.4.peg.322 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L01642 Genomic DNA Translation: AAA23810.1 U00096 Genomic DNA Translation: AAC74994.1 AP009048 Genomic DNA Translation: BAA15755.1 M85240 Genomic DNA No translation available. L13279 Genomic DNA Translation: AAA82575.1 |
PIRi | D64956, A45738 |
RefSeqi | NP_416437.1, NC_000913.3 WP_001245695.1, NZ_LN832404.1 |
3D structure databases
SMRi | P26612 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260380, 11 interactors |
DIPi | DIP-9108N |
IntActi | P26612, 4 interactors |
STRINGi | 511145.b1927 |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Proteomic databases
jPOSTi | P26612 |
PaxDbi | P26612 |
PRIDEi | P26612 |
Genome annotation databases
EnsemblBacteriai | AAC74994; AAC74994; b1927 BAA15755; BAA15755; BAA15755 |
GeneIDi | 946434 |
KEGGi | ecj:JW1912 eco:b1927 |
PATRICi | fig|1411691.4.peg.322 |
Organism-specific databases
EchoBASEi | EB1360 |
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
HOGENOMi | CLU_024572_2_0_6 |
InParanoidi | P26612 |
PhylomeDBi | P26612 |
Enzyme and pathway databases
BioCyci | EcoCyc:ALPHA-AMYL-CYTO-MONOMER MetaCyc:ALPHA-AMYL-CYTO-MONOMER |
Miscellaneous databases
PROi | PR:P26612 |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR013776, A-amylase_thermo IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PIRSFi | PIRSF001021, Alph-amls_thrmst, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMY2_ECOLI | |
Accessioni | P26612Primary (citable) accession number: P26612 Secondary accession number(s): P78072 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries