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UniProtKB - P26602 (UBIC_ECOLI)
Protein
Chorismate pyruvate-lyase
Gene
ubiC
Organism
Escherichia coli (strain K12)
Status
Functioni
Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway.
4 PublicationsMiscellaneous
The sdgG mutation, which suppresses a conditional mutation in dnaG (DNA primase), has been localized to one of the ubiA, ubiC or yjbI genes.1 Publication
Catalytic activityi
- EC:4.1.3.402 Publications
Activity regulationi
Inhibited by 4-hydroxybenzoate, vanillate, 4-hydroxybenzaldehyde and 3-carboxymethylaminomethyl-4-hydroxybenzoic acid.2 Publications
Kineticsi
- KM=6.1 µM for chorismate1 Publication
- KM=29 µM for chorismate1 Publication
pH dependencei
Optimum pH is 7.5.2 Publications
: ubiquinone biosynthesis Pathwayi
This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 35 | Substrate; via amide nitrogen2 Publications | 1 | |
Binding sitei | 77 | Substrate2 Publications | 1 | |
Binding sitei | 115 | Substrate; via amide nitrogen2 Publications | 1 | |
Binding sitei | 156 | Substrate2 Publications | 1 |
GO - Molecular functioni
- chorismate lyase activity Source: EcoCyc
GO - Biological processi
- pyruvate biosynthetic process Source: UniProtKB-UniRule
- ubiquinone biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Ubiquinone biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:CHORPYRLY-MONOMER |
BRENDAi | 4.1.3.40, 2026 |
SABIO-RKi | P26602 |
UniPathwayi | UPA00232 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ubiC Ordered Locus Names:b4039, JW5713 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 91 | G → A: Increases the inhibition by product by about 40%. No effect on substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 156 | E → K: Loss of activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB04242, 4-hydroxybenzoic acid DB02130, Vanillic acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000065711 | 2 – 165 | Chorismate pyruvate-lyaseAdd BLAST | 164 |
Proteomic databases
jPOSTi | P26602 |
PaxDbi | P26602 |
PRIDEi | P26602 |
Interactioni
Subunit structurei
Monomer.
2 PublicationsBinary interactionsi
P26602
With | #Exp. | IntAct |
---|---|---|
aceE [P0AFG8] | 2 | EBI-559360,EBI-542683 |
Protein-protein interaction databases
BioGRIDi | 4262658, 212 interactors 852839, 1 interactor |
DIPi | DIP-11066N |
IntActi | P26602, 3 interactors |
STRINGi | 511145.b4039 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P26602 |
SMRi | P26602 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26602 |
Family & Domainsi
Sequence similaritiesi
Belongs to the UbiC family.Curated
Phylogenomic databases
eggNOGi | COG3161, Bacteria |
HOGENOMi | CLU_096824_1_0_6 |
InParanoidi | P26602 |
OMAi | ELWGRRS |
PhylomeDBi | P26602 |
Family and domain databases
Gene3Di | 3.40.1410.10, 1 hit |
HAMAPi | MF_01632, UbiC, 1 hit |
InterProi | View protein in InterPro IPR007440, Chorismate--pyruvate_lyase IPR028978, Chorismate_lyase_/UTRA_dom_sf |
PANTHERi | PTHR38683, PTHR38683, 1 hit |
Pfami | View protein in Pfam PF04345, Chor_lyase, 1 hit |
SUPFAMi | SSF64288, SSF64288, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P26602-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHPALTQLR ALRYCKEIPA LDPQLLDWLL LEDSMTKRFE QQGKTVSVTM
60 70 80 90 100
IREGFVEQNE IPEELPLLPK ESRYWLREIL LCADGEPWLA GRTVVPVSTL
110 120 130 140 150
SGPELALQKL GKTPLGRYLF TSSTLTRDFI EIGRDAGLWG RRSRLRLSGK
160
PLLLTELFLP ASPLY
Sequence cautioni
The sequence AAC43133 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA40681 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66619 Genomic DNA Translation: CAA47181.1 M93136 Genomic DNA Translation: AAA24711.1 M93413 Genomic DNA Translation: AAA24716.1 X57434 Genomic DNA Translation: CAA40681.1 Different initiation. M96268 Unassigned DNA Translation: AAA17027.1 DQ087228 Genomic DNA Translation: AAY88959.1 U00006 Genomic DNA Translation: AAC43133.1 Different initiation. U00096 Genomic DNA Translation: AAC77009.2 AP009048 Genomic DNA Translation: BAE78041.1 |
PIRi | S25660 |
RefSeqi | NP_418463.4, NC_000913.3 WP_001326644.1, NZ_SSZK01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC77009; AAC77009; b4039 BAE78041; BAE78041; BAE78041 |
GeneIDi | 948545 |
KEGGi | ecj:JW5713 eco:b4039 |
PATRICi | fig|511145.12.peg.4156 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66619 Genomic DNA Translation: CAA47181.1 M93136 Genomic DNA Translation: AAA24711.1 M93413 Genomic DNA Translation: AAA24716.1 X57434 Genomic DNA Translation: CAA40681.1 Different initiation. M96268 Unassigned DNA Translation: AAA17027.1 DQ087228 Genomic DNA Translation: AAY88959.1 U00006 Genomic DNA Translation: AAC43133.1 Different initiation. U00096 Genomic DNA Translation: AAC77009.2 AP009048 Genomic DNA Translation: BAE78041.1 |
PIRi | S25660 |
RefSeqi | NP_418463.4, NC_000913.3 WP_001326644.1, NZ_SSZK01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FW9 | X-ray | 1.40 | A | 2-165 | [»] | |
1G1B | X-ray | 1.99 | A/B | 2-165 | [»] | |
1G81 | X-ray | 1.71 | A | 2-165 | [»] | |
1JD3 | X-ray | 2.03 | A | 2-165 | [»] | |
1TT8 | X-ray | 1.00 | A | 2-165 | [»] | |
1XLR | X-ray | 1.94 | A | 2-165 | [»] | |
2AHC | X-ray | 2.40 | A/B/C/D | 2-165 | [»] | |
AlphaFoldDBi | P26602 | |||||
SMRi | P26602 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262658, 212 interactors 852839, 1 interactor |
DIPi | DIP-11066N |
IntActi | P26602, 3 interactors |
STRINGi | 511145.b4039 |
Chemistry databases
DrugBanki | DB04242, 4-hydroxybenzoic acid DB02130, Vanillic acid |
Proteomic databases
jPOSTi | P26602 |
PaxDbi | P26602 |
PRIDEi | P26602 |
Genome annotation databases
EnsemblBacteriai | AAC77009; AAC77009; b4039 BAE78041; BAE78041; BAE78041 |
GeneIDi | 948545 |
KEGGi | ecj:JW5713 eco:b4039 |
PATRICi | fig|511145.12.peg.4156 |
Organism-specific databases
EchoBASEi | EB1343 |
Phylogenomic databases
eggNOGi | COG3161, Bacteria |
HOGENOMi | CLU_096824_1_0_6 |
InParanoidi | P26602 |
OMAi | ELWGRRS |
PhylomeDBi | P26602 |
Enzyme and pathway databases
UniPathwayi | UPA00232 |
BioCyci | EcoCyc:CHORPYRLY-MONOMER |
BRENDAi | 4.1.3.40, 2026 |
SABIO-RKi | P26602 |
Miscellaneous databases
EvolutionaryTracei | P26602 |
PROi | PR:P26602 |
Family and domain databases
Gene3Di | 3.40.1410.10, 1 hit |
HAMAPi | MF_01632, UbiC, 1 hit |
InterProi | View protein in InterPro IPR007440, Chorismate--pyruvate_lyase IPR028978, Chorismate_lyase_/UTRA_dom_sf |
PANTHERi | PTHR38683, PTHR38683, 1 hit |
Pfami | View protein in Pfam PF04345, Chor_lyase, 1 hit |
SUPFAMi | SSF64288, SSF64288, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBIC_ECOLI | |
Accessioni | P26602Primary (citable) accession number: P26602 Secondary accession number(s): P76783, Q2M6R5, Q4JHR8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families