UniProtKB - P26599 (PTBP1_HUMAN)
Protein
Polypyrimidine tract-binding protein 1
Gene
PTBP1
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).6 Publications
GO - Molecular functioni
- mRNA binding Source: GO_Central
- poly-pyrimidine tract binding Source: ProtInc
- pre-mRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- fibroblast growth factor receptor signaling pathway Source: Reactome
- IRES-dependent viral translational initiation Source: FlyBase
- mRNA processing Source: ProtInc
- mRNA splicing, via spliceosome Source: Reactome
- negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
- negative regulation of muscle cell differentiation Source: UniProtKB
- negative regulation of RNA splicing Source: UniProtKB
- positive regulation of calcineurin-NFAT signaling cascade Source: BHF-UCL
- positive regulation of protein dephosphorylation Source: BHF-UCL
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- regulation of cell differentiation Source: GO_Central
- regulation of RNA splicing Source: GO_Central
- RNA metabolic process Source: Reactome
- RNA splicing Source: ProtInc
Keywordsi
Molecular function | Activator, Repressor, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | P26599 |
Reactomei | R-HSA-6803529, FGFR2 alternative splicing R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA |
Names & Taxonomyi
Protein namesi | Recommended name: Polypyrimidine tract-binding protein 1Short name: PTB Alternative name(s): 57 kDa RNA-binding protein PPTB-1 Heterogeneous nuclear ribonucleoprotein I Short name: hnRNP I |
Gene namesi | Name:PTBP1 Synonyms:PTB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9583, PTBP1 |
MIMi | 600693, gene |
neXtProti | NX_P26599 |
VEuPathDBi | HostDB:ENSG00000011304.17 |
Subcellular locationi
Nucleus
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleolus Source: ProtInc
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- membrane Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 5725 |
OpenTargetsi | ENSG00000011304 |
PharmGKBi | PA33934 |
Miscellaneous databases
Pharosi | P26599, Tbio |
Chemistry databases
ChEMBLi | CHEMBL1293230 |
Genetic variation databases
BioMutai | PTBP1 |
DMDMi | 131528 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081737 | 1 – 531 | Polypyrimidine tract-binding protein 1Add BLAST | 531 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources1 Publication | 1 | |
Modified residuei | 16 | PhosphoserineCombined sources | 1 | |
Cross-linki | 65 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 127 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 138 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 141 | PhosphoserineCombined sources | 1 | |
Cross-linki | 218 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 433 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P26599 |
jPOSTi | P26599 |
MassIVEi | P26599 |
MaxQBi | P26599 |
PaxDbi | P26599 |
PeptideAtlasi | P26599 |
PRIDEi | P26599 |
ProteomicsDBi | 54354 [P26599-1] 54355 [P26599-2] 54356 [P26599-3] |
TopDownProteomicsi | P26599-1 [P26599-1] P26599-2 [P26599-2] |
2D gel databases
SWISS-2DPAGEi | P26599 |
PTM databases
iPTMneti | P26599 |
MetOSitei | P26599 |
PhosphoSitePlusi | P26599 |
SwissPalmi | P26599 |
Expressioni
Gene expression databases
Bgeei | ENSG00000011304, Expressed in embryo and 253 other tissues |
ExpressionAtlasi | P26599, baseline and differential |
Genevisiblei | P26599, HS |
Organism-specific databases
HPAi | ENSG00000011304, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1.
Interacts with RAVER1 and SFPQ.
3 PublicationsBinary interactionsi
Hide detailsP26599
Isoform 3 [P26599-3]
With | #Exp. | IntAct |
---|---|---|
RBM10 [P98175] | 3 | EBI-16437588,EBI-721525 |
TARDBP [Q13148] | 3 | EBI-16437588,EBI-372899 |
Protein-protein interaction databases
BioGRIDi | 111697, 302 interactors |
CORUMi | P26599 |
ELMi | P26599 |
IntActi | P26599, 84 interactors |
MINTi | P26599 |
STRINGi | 9606.ENSP00000349428 |
Miscellaneous databases
RNActi | P26599, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P26599 |
SASBDBi | P26599 |
SMRi | P26599 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26599 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 59 – 143 | RRM 1PROSITE-ProRule annotationAdd BLAST | 85 | |
Domaini | 184 – 260 | RRM 2PROSITE-ProRule annotationAdd BLAST | 77 | |
Domaini | 337 – 411 | RRM 3PROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 454 – 529 | RRM 4PROSITE-ProRule annotationAdd BLAST | 76 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 316 – 323 | Poly-Ala | 8 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1190, Eukaryota |
GeneTreei | ENSGT00990000203586 |
HOGENOMi | CLU_015171_7_1_1 |
InParanoidi | P26599 |
OMAi | LTIIPAC |
OrthoDBi | 1545178at2759 |
PhylomeDBi | P26599 |
TreeFami | TF319824 |
Family and domain databases
CDDi | cd12777, RRM1_PTBP1, 1 hit cd12693, RRM2_PTBP1_like, 1 hit cd12695, RRM3_PTBP1, 1 hit |
DisProti | DP01601 |
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR006536, HnRNP-L/PTB IPR012677, Nucleotide-bd_a/b_plait_sf IPR034798, PTBP1/2/3_RRM2 IPR021790, PTBP1_RRM IPR035000, PTBP1_RRM1 IPR035001, PTBP1_RRM3 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF11835, RRM_8, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 4 hits |
SUPFAMi | SSF54928, SSF54928, 4 hits |
TIGRFAMsi | TIGR01649, hnRNP-L_PTB, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 4 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 3 described isoforms and 13 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P26599-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDGIVPDIAV GTKRGSDELF STCVTNGPFI MSSNSASAAN GNDSKKFKGD
60 70 80 90 100
SRSAGVPSRV IHIRKLPIDV TEGEVISLGL PFGKVTNLLM LKGKNQAFIE
110 120 130 140 150
MNTEEAANTM VNYYTSVTPV LRGQPIYIQF SNHKELKTDS SPNQARAQAA
160 170 180 190 200
LQAVNSVQSG NLALAASAAA VDAGMAMAGQ SPVLRIIVEN LFYPVTLDVL
210 220 230 240 250
HQIFSKFGTV LKIITFTKNN QFQALLQYAD PVSAQHAKLS LDGQNIYNAC
260 270 280 290 300
CTLRIDFSKL TSLNVKYNND KSRDYTRPDL PSGDSQPSLD QTMAAAFGLS
310 320 330 340 350
VPNVHGALAP LAIPSAAAAA AAAGRIAIPG LAGAGNSVLL VSNLNPERVT
360 370 380 390 400
PQSLFILFGV YGDVQRVKIL FNKKENALVQ MADGNQAQLA MSHLNGHKLH
410 420 430 440 450
GKPIRITLSK HQNVQLPREG QEDQGLTKDY GNSPLHRFKK PGSKNFQNIF
460 470 480 490 500
PPSATLHLSN IPPSVSEEDL KVLFSSNGGV VKGFKFFQKD RKMALIQMGS
510 520 530
VEEAVQALID LHNHDLGENH HLRVSFSKST I
Computationally mapped potential isoform sequencesi
There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6NLN1 | A6NLN1_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 hCG_20560 | 527 | Annotation score: | ||
A0A0U1RRM4 | A0A0U1RRM4_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 588 | Annotation score: | ||
A0A6Q8PEX4 | A0A6Q8PEX4_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 339 | Annotation score: | ||
A0A087WUW5 | A0A087WUW5_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 81 | Annotation score: | ||
A0A0D9SF20 | A0A0D9SF20_HUMAN | Polypyrimidine tract binding protei... | PTBP1 hCG_20560 | 197 | Annotation score: | ||
K7ES59 | K7ES59_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 149 | Annotation score: | ||
K7EK45 | K7EK45_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 256 | Annotation score: | ||
K7EKJ7 | K7EKJ7_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 237 | Annotation score: | ||
A0A087WU68 | A0A087WU68_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 227 | Annotation score: | ||
K7ELW5 | K7ELW5_HUMAN | Polypyrimidine tract-binding protei... | PTBP1 | 105 | Annotation score: | ||
There are more potential isoformsShow all |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005802 | 297 | F → FASPYAGAGFPPTFAIPQAA in isoform 2. Curated | 1 | |
Alternative sequenceiVSP_043649 | 297 | F → FGAPGIISASPYAGAGFPPT FAIPQAA in isoform 3. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62006 mRNA Translation: CAA43973.1 X65371 mRNA Translation: CAA46443.1 X65372 mRNA Translation: CAA46444.1 X60648 mRNA Translation: CAA43056.1 X66975 mRNA Translation: CAA47386.1 BT006819 mRNA Translation: AAP35465.1 AC006273 Genomic DNA Translation: AAC99798.1 CH471242 Genomic DNA Translation: EAW61154.1 BC002397 mRNA Translation: AAH02397.1 BC004383 mRNA Translation: AAH04383.1 BC013694 mRNA Translation: AAH13694.1 |
CCDSi | CCDS32859.1 [P26599-1] CCDS42456.1 [P26599-3] CCDS45892.1 [P26599-2] |
PIRi | S23016 |
RefSeqi | NP_002810.1, NM_002819.4 [P26599-3] NP_114367.1, NM_031990.3 [P26599-2] NP_114368.1, NM_031991.3 [P26599-1] |
Genome annotation databases
Ensembli | ENST00000349038; ENSP00000014112; ENSG00000011304 [P26599-1] ENST00000356948; ENSP00000349428; ENSG00000011304 [P26599-3] ENST00000394601; ENSP00000408096; ENSG00000011304 [P26599-2] |
GeneIDi | 5725 |
KEGGi | hsa:5725 |
UCSCi | uc002lpp.3, human [P26599-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62006 mRNA Translation: CAA43973.1 X65371 mRNA Translation: CAA46443.1 X65372 mRNA Translation: CAA46444.1 X60648 mRNA Translation: CAA43056.1 X66975 mRNA Translation: CAA47386.1 BT006819 mRNA Translation: AAP35465.1 AC006273 Genomic DNA Translation: AAC99798.1 CH471242 Genomic DNA Translation: EAW61154.1 BC002397 mRNA Translation: AAH02397.1 BC004383 mRNA Translation: AAH04383.1 BC013694 mRNA Translation: AAH13694.1 |
CCDSi | CCDS32859.1 [P26599-1] CCDS42456.1 [P26599-3] CCDS45892.1 [P26599-2] |
PIRi | S23016 |
RefSeqi | NP_002810.1, NM_002819.4 [P26599-3] NP_114367.1, NM_031990.3 [P26599-2] NP_114368.1, NM_031991.3 [P26599-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QM9 | NMR | - | A | 335-531 | [»] | |
1SJQ | NMR | - | A | 55-147 | [»] | |
1SJR | NMR | - | A | 147-301 | [»] | |
2AD9 | NMR | - | A | 49-146 | [»] | |
2ADB | NMR | - | A | 172-298 | [»] | |
2ADC | NMR | - | A | 324-531 | [»] | |
2EVZ | NMR | - | A | 324-531 | [»] | |
2N3O | NMR | - | A | 41-163 | [»] | |
3ZZY | X-ray | 1.40 | A/B | 156-285 | [»] | |
3ZZZ | X-ray | 1.55 | A/B | 156-285 | [»] | |
BMRBi | P26599 | |||||
SASBDBi | P26599 | |||||
SMRi | P26599 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111697, 302 interactors |
CORUMi | P26599 |
ELMi | P26599 |
IntActi | P26599, 84 interactors |
MINTi | P26599 |
STRINGi | 9606.ENSP00000349428 |
Chemistry databases
ChEMBLi | CHEMBL1293230 |
PTM databases
iPTMneti | P26599 |
MetOSitei | P26599 |
PhosphoSitePlusi | P26599 |
SwissPalmi | P26599 |
Genetic variation databases
BioMutai | PTBP1 |
DMDMi | 131528 |
2D gel databases
SWISS-2DPAGEi | P26599 |
Proteomic databases
EPDi | P26599 |
jPOSTi | P26599 |
MassIVEi | P26599 |
MaxQBi | P26599 |
PaxDbi | P26599 |
PeptideAtlasi | P26599 |
PRIDEi | P26599 |
ProteomicsDBi | 54354 [P26599-1] 54355 [P26599-2] 54356 [P26599-3] |
TopDownProteomicsi | P26599-1 [P26599-1] P26599-2 [P26599-2] |
Protocols and materials databases
Antibodypediai | 4613, 431 antibodies |
DNASUi | 5725 |
Genome annotation databases
Ensembli | ENST00000349038; ENSP00000014112; ENSG00000011304 [P26599-1] ENST00000356948; ENSP00000349428; ENSG00000011304 [P26599-3] ENST00000394601; ENSP00000408096; ENSG00000011304 [P26599-2] |
GeneIDi | 5725 |
KEGGi | hsa:5725 |
UCSCi | uc002lpp.3, human [P26599-1] |
Organism-specific databases
CTDi | 5725 |
DisGeNETi | 5725 |
GeneCardsi | PTBP1 |
HGNCi | HGNC:9583, PTBP1 |
HPAi | ENSG00000011304, Low tissue specificity |
MIMi | 600693, gene |
neXtProti | NX_P26599 |
OpenTargetsi | ENSG00000011304 |
PharmGKBi | PA33934 |
VEuPathDBi | HostDB:ENSG00000011304.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1190, Eukaryota |
GeneTreei | ENSGT00990000203586 |
HOGENOMi | CLU_015171_7_1_1 |
InParanoidi | P26599 |
OMAi | LTIIPAC |
OrthoDBi | 1545178at2759 |
PhylomeDBi | P26599 |
TreeFami | TF319824 |
Enzyme and pathway databases
PathwayCommonsi | P26599 |
Reactomei | R-HSA-6803529, FGFR2 alternative splicing R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA |
Miscellaneous databases
BioGRID-ORCSi | 5725, 387 hits in 1017 CRISPR screens |
ChiTaRSi | PTBP1, human |
EvolutionaryTracei | P26599 |
GeneWikii | PTBP1 |
GenomeRNAii | 5725 |
Pharosi | P26599, Tbio |
PROi | PR:P26599 |
RNActi | P26599, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000011304, Expressed in embryo and 253 other tissues |
ExpressionAtlasi | P26599, baseline and differential |
Genevisiblei | P26599, HS |
Family and domain databases
CDDi | cd12777, RRM1_PTBP1, 1 hit cd12693, RRM2_PTBP1_like, 1 hit cd12695, RRM3_PTBP1, 1 hit |
DisProti | DP01601 |
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR006536, HnRNP-L/PTB IPR012677, Nucleotide-bd_a/b_plait_sf IPR034798, PTBP1/2/3_RRM2 IPR021790, PTBP1_RRM IPR035000, PTBP1_RRM1 IPR035001, PTBP1_RRM3 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF11835, RRM_8, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 4 hits |
SUPFAMi | SSF54928, SSF54928, 4 hits |
TIGRFAMsi | TIGR01649, hnRNP-L_PTB, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTBP1_HUMAN | |
Accessioni | P26599Primary (citable) accession number: P26599 Secondary accession number(s): Q9BUQ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 233 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references