UniProtKB - P26449 (BUB3_YEAST)
Protein
Cell cycle arrest protein BUB3
Gene
BUB3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for cell cycle arrest in response to loss of microtubule function. Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism.1 Publication
Miscellaneous
Present with 1430 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- ubiquitin binding Source: SGD
GO - Biological processi
- mitotic DNA integrity checkpoint Source: SGD
- mitotic spindle assembly checkpoint Source: GO_Central
- positive regulation of protein autoubiquitination Source: SGD
Keywordsi
Biological process | Cell cycle |
Enzyme and pathway databases
Reactomei | R-SCE-141430, Inactivation of APC/C via direct inhibition of the APC/C complex |
Names & Taxonomyi
Protein namesi | Recommended name: Cell cycle arrest protein BUB3 |
Gene namesi | Name:BUB3 Ordered Locus Names:YOR026W ORF Names:OR26.16 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOR026W |
SGDi | S000005552, BUB3 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- condensed nuclear chromosome kinetochore Source: SGD
- mitotic checkpoint complex Source: SGD
Other locations
- bub1-bub3 complex Source: GO_Central
- kinetochore Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | Q → L: Abolishes checkpoint function. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 31 | W → G: Abolishes checkpoint function and interaction with MAD2, MAD3 and CDC20. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 120 | W → G: Abolishes checkpoint function and interaction with MAD2, MAD3 and CDC20. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 188 | E → V: Abolishes checkpoint function. No effect on interaction with BUB1 and MAD3. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 191 | G → R: Abolishes checkpoint function. No effect on interaction with BUB1 and MAD3. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 192 | L → E: Abolishes checkpoint function. No effect on interaction with BUB1 and MAD3. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 193 | K → T: Abolishes checkpoint function. No effect on interaction with BUB1 and MAD3. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 217 | R → E: Abolishes checkpoint function. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 226 | Q → L: Abolishes checkpoint function. No effect on interaction with BUB1 and MAD3. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 242 | R → E: Abolishes checkpoint function. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 276 | S → P: Abolishes checkpoint function. Lowers interaction with BUB1 and MAD3. Benomyl-sensitive phenotype. 1 Publication | 1 | |
Mutagenesisi | 278 | W → R: Abolishes checkpoint function. No effect on interaction with BUB1. Lowers interaction with MAD3. Benomyl-sensitive phenotype. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050890 | 1 – 341 | Cell cycle arrest protein BUB3Add BLAST | 341 |
Post-translational modificationi
Phosphorylated by BUB1.
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P26449 |
PaxDbi | P26449 |
PTM databases
iPTMneti | P26449 |
Interactioni
Subunit structurei
Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20. Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint.
Interacts with MAD1, MAD2 and CDC20.
3 PublicationsBinary interactionsi
Hide detailsP26449
With | #Exp. | IntAct |
---|---|---|
BUB1 [P41695] | 12 | EBI-3830,EBI-3816 |
CDC20 [P26309] | 8 | EBI-3830,EBI-4212 |
MAD1 [P40957] | 4 | EBI-3830,EBI-10354 |
MAD2 [P40958] | 4 | EBI-3830,EBI-10362 |
MAD3 [P47074] | 14 | EBI-3830,EBI-10369 |
GO - Molecular functioni
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 34429, 706 interactors |
ComplexPortali | CPX-154, Bub1-Bub3 complex CPX-3212, Mitotic checkpoint complex, MAD1-MAD2-BUB1-BUB3 subcomplex CPX-963, Mitotic checkpoint Complex |
DIPi | DIP-1219N |
IntActi | P26449, 10 interactors |
MINTi | P26449 |
STRINGi | 4932.YOR026W |
Miscellaneous databases
RNActi | P26449, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P26449 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26449 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 9 – 48 | WD 1Add BLAST | 40 | |
Repeati | 54 – 96 | WD 2Add BLAST | 43 | |
Repeati | 97 – 137 | WD 3Add BLAST | 41 | |
Repeati | 144 – 185 | WD 4Add BLAST | 42 | |
Repeati | 191 – 233 | WD 5Add BLAST | 43 | |
Repeati | 249 – 288 | WD 6Add BLAST | 40 | |
Repeati | 292 – 329 | WD 7Add BLAST | 38 |
Sequence similaritiesi
Belongs to the WD repeat BUB3 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG1036, Eukaryota |
GeneTreei | ENSGT00950000183091 |
HOGENOMi | CLU_038526_2_0_1 |
InParanoidi | P26449 |
OMAi | ENECKPK |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 4 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
P26449-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQIVQIEQAP KDYISDIKII PSKSLLLITS WDGSLTVYKF DIQAKNVDLL
60 70 80 90 100
QSLRYKHPLL CCNFIDNTDL QIYVGTVQGE ILKVDLIGSP SFQALTNNEA
110 120 130 140 150
NLGICRICKY GDDKLIAASW DGLIEVIDPR NYGDGVIAVK NLNSNNTKVK
160 170 180 190 200
NKIFTMDTNS SRLIVGMNNS QVQWFRLPLC EDDNGTIEES GLKYQIRDVA
210 220 230 240 250
LLPKEQEGYA CSSIDGRVAV EFFDDQGDDY NSSKRFAFRC HRLNLKDTNL
260 270 280 290 300
AYPVNSIEFS PRHKFLYTAG SDGIISCWNL QTRKKIKNFA KFNEDSVVKI
310 320 330 340
ACSDNILCLA TSDDTFKTNA AIDQTIELNA SSIYIIFDYE N
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64707 Genomic DNA Translation: AAA34459.1 X87331 Genomic DNA Translation: CAA60742.1 Z74934 Genomic DNA Translation: CAA99216.1 BK006948 Genomic DNA Translation: DAA10808.1 |
PIRi | B39654 |
RefSeqi | NP_014669.1, NM_001183445.1 |
Genome annotation databases
EnsemblFungii | YOR026W_mRNA; YOR026W; YOR026W |
GeneIDi | 854191 |
KEGGi | sce:YOR026W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64707 Genomic DNA Translation: AAA34459.1 X87331 Genomic DNA Translation: CAA60742.1 Z74934 Genomic DNA Translation: CAA99216.1 BK006948 Genomic DNA Translation: DAA10808.1 |
PIRi | B39654 |
RefSeqi | NP_014669.1, NM_001183445.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1U4C | X-ray | 2.35 | A/B | 1-341 | [»] | |
1YFQ | X-ray | 1.10 | A | 1-341 | [»] | |
2I3S | X-ray | 1.90 | A/C/E | 1-341 | [»] | |
2I3T | X-ray | 2.80 | A/C/E/G | 1-341 | [»] | |
4BL0 | X-ray | 1.95 | A/D | 1-341 | [»] | |
SMRi | P26449 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34429, 706 interactors |
ComplexPortali | CPX-154, Bub1-Bub3 complex CPX-3212, Mitotic checkpoint complex, MAD1-MAD2-BUB1-BUB3 subcomplex CPX-963, Mitotic checkpoint Complex |
DIPi | DIP-1219N |
IntActi | P26449, 10 interactors |
MINTi | P26449 |
STRINGi | 4932.YOR026W |
PTM databases
iPTMneti | P26449 |
Proteomic databases
MaxQBi | P26449 |
PaxDbi | P26449 |
Genome annotation databases
EnsemblFungii | YOR026W_mRNA; YOR026W; YOR026W |
GeneIDi | 854191 |
KEGGi | sce:YOR026W |
Organism-specific databases
EuPathDBi | FungiDB:YOR026W |
SGDi | S000005552, BUB3 |
Phylogenomic databases
eggNOGi | KOG1036, Eukaryota |
GeneTreei | ENSGT00950000183091 |
HOGENOMi | CLU_038526_2_0_1 |
InParanoidi | P26449 |
OMAi | ENECKPK |
Enzyme and pathway databases
Reactomei | R-SCE-141430, Inactivation of APC/C via direct inhibition of the APC/C complex |
Miscellaneous databases
EvolutionaryTracei | P26449 |
PROi | PR:P26449 |
RNActi | P26449, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 4 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BUB3_YEAST | |
Accessioni | P26449Primary (citable) accession number: P26449 Secondary accession number(s): D6W292 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD