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UniProtKB - P26368 (U2AF2_HUMAN)

Entry version 226 (02 Dec 2020)
Sequence version 4 (23 Jan 2007)
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Protein

Splicing factor U2AF 65 kDa subunit

Gene

U2AF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Plays a role in pre-mRNA splicing and 3'-end processing (PubMed:17024186). By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).5 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRepressor, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P26368

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72163, mRNA Splicing - Major Pathway
R-HSA-72187, mRNA 3'-end processing
R-HSA-73856, RNA Polymerase II Transcription Termination

SIGNOR Signaling Network Open Resource

More...SIGNORi		P26368

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Splicing factor U2AF 65 kDa subunit
Alternative name(s):
U2 auxiliary factor 65 kDa subunit
Short name:
hU2AF(65)
Short name:
hU2AF65
U2 snRNP auxiliary factor large subunit
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:U2AF2
Synonyms:U2AF65
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Eukaryotic Pathogen and Host Database Resources

More...EuPathDBi		HostDB:ENSG00000063244.12

Human Gene Nomenclature Database

More...HGNCi		HGNC:23156, U2AF2

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		191318, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P26368

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi92W → A: Decreases affinity for UAF1 by 3 orders of magnitude. 1 Publication1
Mutagenesisi96P → G: Decreases affinity for UAF1 by 2 orders of magnitude. 1 Publication1
Mutagenesisi104P → G: Decreases affinity for UAF1 by 2 orders of magnitude. 1 Publication1
Mutagenesisi387 – 388EE → RR: Reduces interaction with SF1. 1 Publication2
Mutagenesisi391 – 394DDEE → AAAA: Reduces interaction with SF1. 4
Mutagenesisi391 – 394DDEE → RRKK: Reduces interaction with SF1. 4
Mutagenesisi396 – 397EE → AA: No effect. 1 Publication2
Mutagenesisi396 – 397EE → GA: Reduces interaction with SF1. 1 Publication2
Mutagenesisi396 – 397EE → KK: Reduces interaction with SF1. 1 Publication2
Mutagenesisi454F → A: Reduces interaction with SF1. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		11338

Open Targets

More...OpenTargetsi		ENSG00000063244

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA134908683

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P26368, Tbio

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		U2AF2

Domain mapping of disease mutations (DMDM)

More...DMDMi		267188

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources1 Publication
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000819882 – 475Splicing factor U2AF 65 kDa subunitAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei2PhosphoserineCombined sources1
Modified residuei155-hydroxylysine; by JMJD6; alternate1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei70N6-acetyllysine; alternateCombined sources1
Cross-linki70Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei79PhosphoserineCombined sources1
Modified residuei2765-hydroxylysine; by JMJD61 Publication1
Modified residuei294PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.1 Publication

Keywords - PTMi

Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P26368

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P26368

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P26368

MaxQB - The MaxQuant DataBase

More...MaxQBi		P26368

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P26368

PeptideAtlas

More...PeptideAtlasi		P26368

PRoteomics IDEntifications database

More...PRIDEi		P26368

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		54325 [P26368-1]
54326 [P26368-2]

Consortium for Top Down Proteomics

More...TopDownProteomicsi		P26368-1 [P26368-1]
P26368-2 [P26368-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P26368

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P26368

SwissPalm database of S-palmitoylation events

More...SwissPalmi		P26368

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000063244, Expressed in right testis and 232 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P26368, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P26368, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000063244, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with U2AF1L4 (By similarity). Heterodimer with U2AF1 (PubMed:11551507). Binds unphosphorylated SF1 (PubMed:10449420, PubMed:12718882).

Interacts with SCAF11 and SNW1 (PubMed:9447963, PubMed:21460037).

Interacts with ZRSR2/U2AF1-RS2.

Interacts with RBM17 (PubMed:17589525).

Interacts with PRPF19; the interaction is direct.

Interacts with POLR2A (via the C-terminal domain); recruits PRPF19 and the Prp19 complex to the pre-mRNA (PubMed:21536736).

Interacts with KHDC4 (Isoform 2) (PubMed:19641227).

Interacts with ZRSR2 (PubMed:9237760).

Interacts with the SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A (PubMed:27720643).

Interacts (via N-terminus) with CPSF7 (via C-terminus); this interaction stimulates pre-mRNA 3'-end processing by promoting the recruitment of the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).

By similarity12 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details
P26368
With#Exp.IntAct
ATXN1 [P54253]5EBI-742339,EBI-930964
CHERP [Q8IWX8]2EBI-742339,EBI-2555370
CPSF7 [Q8N684]2EBI-742339,EBI-746909
DACH1 - isoform 2 [Q9UI36-2]3EBI-742339,EBI-10186082
MUC1 [P15941]2EBI-742339,EBI-2804728
PRPF3 [O43395]2EBI-742339,EBI-744322
PUF60 [Q9UHX1]5EBI-742339,EBI-1053259
RBM10 [P98175]2EBI-742339,EBI-721525
RBM17 [Q96I25]2EBI-742339,EBI-740272
RBM5 [P52756]2EBI-742339,EBI-714003
SARNP [P82979]3EBI-742339,EBI-347495
SF1 [Q15637]22EBI-742339,EBI-744603
SNRPA [P09012]5EBI-742339,EBI-607085
SNW1 [Q13573]5EBI-742339,EBI-632715
SRPK2 [P78362]4EBI-742339,EBI-593303
SUGP1 [Q8IWZ8]3EBI-742339,EBI-2691671
THAP1 [Q9NVV9]4EBI-742339,EBI-741515
TLE5 [Q08117]3EBI-742339,EBI-717810
U2AF1 [Q01081]11EBI-742339,EBI-632461
Isoform 2 [P26368-2]
With#Exp.IntAct
DDX39B [Q13838]3EBI-11097439,EBI-348622
FUBP1 - isoform 2 [Q96AE4-2]3EBI-11097439,EBI-12121668
HMBOX1 [Q6NT76]3EBI-11097439,EBI-2549423
INO80B [Q9C086]3EBI-11097439,EBI-715611
KHDC4 - isoform 4 [Q7Z7F0-4]3EBI-11097439,EBI-9089060
MRPL15 [Q9P015]3EBI-11097439,EBI-2371967
NKAPD1 [Q6ZUT1]3EBI-11097439,EBI-3920396
PRPF3 [O43395]3EBI-11097439,EBI-744322
PUF60 - isoform 2 [Q9UHX1-2]3EBI-11097439,EBI-11529177
RBM11 [P57052]3EBI-11097439,EBI-741332
RBMY1J [Q15415]3EBI-11097439,EBI-8642021
SF1 - isoform 4 [Q15637-4]5EBI-11097439,EBI-12223157
SNRPA [P09012]6EBI-11097439,EBI-607085
SRPK2 [P78362]3EBI-11097439,EBI-593303
SRSF3 [P84103]3EBI-11097439,EBI-372557
STAC3 [Q96MF2]3EBI-11097439,EBI-745680
TCF23 [Q7RTU1]3EBI-11097439,EBI-12127592
TLE5 - isoform 2 [Q08117-2]3EBI-11097439,EBI-11741437
U2AF1L5 [P0DN76]7EBI-11097439,EBI-14082807
ZNF107 [Q9UII5]3EBI-11097439,EBI-7234993
ZNF526 [Q8TF50]3EBI-11097439,EBI-11035148
ZNF57 [Q68EA5]3EBI-11097439,EBI-8490788
ZNF71 [Q9NQZ8]3EBI-11097439,EBI-7138235
ZNF835 [Q9Y2P0]3EBI-11097439,EBI-5667516
ZRSR2P1 [Q15695]3EBI-11097439,EBI-12270264

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		116466, 435 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-1074, SF1-U2AF65 splicing factor complex

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		P26368

Database of interacting proteins

More...DIPi		DIP-2154N

Protein interaction database and analysis system

More...IntActi		P26368, 126 interactors

Molecular INTeraction database

More...MINTi		P26368

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000307863

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P26368, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1475
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		P26368

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P26368

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P26368

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini149 – 231RRM 1PROSITE-ProRule annotationAdd BLAST83
Domaini259 – 337RRM 2PROSITE-ProRule annotationAdd BLAST79
Domaini385 – 466RRM 3PROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni2 – 93Required for interaction with PRPF191 PublicationAdd BLAST92
Regioni17 – 47Necessary and sufficient to stimulate pre-mRNAs 3'-end cleavage in a CFIm complex-dependent manner1 PublicationAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi27 – 62Arg/Ser-rich (RS domain)Add BLAST36

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0120, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000155556

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P26368

Identification of Orthologs from Complete Genome Data

More...OMAi		RRMTQWD

Database of Orthologous Groups

More...OrthoDBi		896650at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P26368

TreeFam database of animal gene trees

More...TreeFami		TF314111

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.30.70.330, 3 hits

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...IDEALi		IID00147

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR012677, Nucleotide-bd_a/b_plait_sf
IPR035979, RBD_domain_sf
IPR000504, RRM_dom
IPR006529, U2AF_lg

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00076, RRM_1, 3 hits

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00360, RRM, 3 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF54928, SSF54928, 3 hits

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01642, U2AF_lg, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50102, RRM, 3 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P26368-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDFDEFERQ LNENKQERDK ENRHRKRSHS RSRSRDRKRR SRSRDRRNRD 
        60         70         80         90        100
QRSASRDRRR RSKPLTRGAK EEHGGLIRSP RHEKKKKVRK YWDVPPPGFE 
       110        120        130        140        150
HITPMQYKAM QAAGQIPATA LLPTMTPDGL AVTPTPVPVV GSQMTRQARR 
       160        170        180        190        200
LYVGNIPFGI TEEAMMDFFN AQMRLGGLTQ APGNPVLAVQ INQDKNFAFL 
       210        220        230        240        250
EFRSVDETTQ AMAFDGIIFQ GQSLKIRRPH DYQPLPGMSE NPSVYVPGVV 
       260        270        280        290        300
STVVPDSAHK LFIGGLPNYL NDDQVKELLT SFGPLKAFNL VKDSATGLSK 
       310        320        330        340        350
GYAFCEYVDI NVTDQAIAGL NGMQLGDKKL LVQRASVGAK NATLVSPPST 
       360        370        380        390        400
INQTPVTLQV PGLMSSQVQM GGHPTEVLCL MNMVLPEELL DDEEYEEIVE 
       410        420        430        440        450
DVRDECSKYG LVKSIEIPRP VDGVEVPGCG KIFVEFTSVF DCQKAMQGLT 
       460        470 
GRKFANRVVV TKYCDPDSYH RRDFW
Length:475
Mass (Da):53,501
Last modified:January 23, 2007 - v4
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i26AD271CD8FC6211
GO
Isoform 2 (identifier: P26368-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     345-348: Missing.

Show »
Length:471
Mass (Da):53,121
Checksum:i3F59A02CD2B03F46
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7ENG2K7ENG2_HUMAN
U2 snRNP auxiliary factor large sub...
U2AF2
307Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QYQ9M0QYQ9_HUMAN
Splicing factor U2AF 65 kDa subunit
U2AF2
51Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
K7ELP8K7ELP8_HUMAN
Splicing factor U2AF 65 kDa subunit
U2AF2
86Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_035414345 – 348Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X64044 mRNA Translation: CAA45409.1
CH471135 Genomic DNA Translation: EAW72404.1
BC008740 mRNA Translation: AAH08740.1
BC030574 mRNA Translation: AAH30574.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS12933.1 [P26368-1]
CCDS46197.1 [P26368-2]

Protein sequence database of the Protein Information Resource

More...PIRi		S20250

NCBI Reference Sequences

More...RefSeqi		NP_001012496.1, NM_001012478.1 [P26368-2]
NP_009210.1, NM_007279.2 [P26368-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000308924; ENSP00000307863; ENSG00000063244 [P26368-1]
ENST00000450554; ENSP00000388475; ENSG00000063244 [P26368-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		11338

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:11338

UCSC genome browser

More...UCSCi		uc002qlt.4, human [P26368-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64044 mRNA Translation: CAA45409.1
CH471135 Genomic DNA Translation: EAW72404.1
BC008740 mRNA Translation: AAH08740.1
BC030574 mRNA Translation: AAH30574.1
CCDSiCCDS12933.1 [P26368-1]
CCDS46197.1 [P26368-2]
PIRiS20250
RefSeqiNP_001012496.1, NM_001012478.1 [P26368-2]
NP_009210.1, NM_007279.2 [P26368-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JMTX-ray2.20B85-112[»]
1O0PNMR-A372-475[»]
1OPINMR-A372-475[»]
1U2FNMR-A148-237[»]
2G4BX-ray2.50A148-336[»]
2HZCX-ray1.47A148-229[»]
2M0GNMR-B372-475[»]
2U2FNMR-A258-342[»]
2YH0NMR-A148-342[»]
2YH1NMR-A148-342[»]
3VAFX-ray2.49A/B148-336[»]
3VAGX-ray2.19A/B148-336[»]
3VAHX-ray2.50A/B148-336[»]
3VAIX-ray2.20A/B148-336[»]
3VAJX-ray1.90A/B148-336[»]
3VAKX-ray2.17A/B148-336[»]
3VALX-ray2.50A/B/D/I148-336[»]
3VAMX-ray2.40A/B148-336[»]
4FXWX-ray2.29A/C375-475[»]
4TU7X-ray2.09A/B148-336[»]
4TU8X-ray1.92A/B148-336[»]
4TU9X-ray1.99A/B148-336[»]
5EV1X-ray2.04A141-341[»]
5EV2X-ray1.86A141-341[»]
5EV3X-ray1.50A141-341[»]
5EV4X-ray1.57A141-341[»]
5W0GX-ray1.07A148-229[»]
5W0HX-ray1.11A258-336[»]
6TR0NMR-A140-342[»]
BMRBiP26368
SMRiP26368
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi116466, 435 interactors
ComplexPortaliCPX-1074, SF1-U2AF65 splicing factor complex
CORUMiP26368
DIPiDIP-2154N
IntActiP26368, 126 interactors
MINTiP26368
STRINGi9606.ENSP00000307863

PTM databases

iPTMnetiP26368
PhosphoSitePlusiP26368
SwissPalmiP26368

Polymorphism and mutation databases

BioMutaiU2AF2
DMDMi267188

Proteomic databases

EPDiP26368
jPOSTiP26368
MassIVEiP26368
MaxQBiP26368
PaxDbiP26368
PeptideAtlasiP26368
PRIDEiP26368
ProteomicsDBi54325 [P26368-1]
54326 [P26368-2]
TopDownProteomicsiP26368-1 [P26368-1]
P26368-2 [P26368-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		4399, 247 antibodies

The DNASU plasmid repository

More...DNASUi		11338

Genome annotation databases

EnsembliENST00000308924; ENSP00000307863; ENSG00000063244 [P26368-1]
ENST00000450554; ENSP00000388475; ENSG00000063244 [P26368-2]
GeneIDi11338
KEGGihsa:11338
UCSCiuc002qlt.4, human [P26368-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		11338
DisGeNETi11338
EuPathDBiHostDB:ENSG00000063244.12

GeneCards: human genes, protein and diseases

More...GeneCardsi		U2AF2
HGNCiHGNC:23156, U2AF2
HPAiENSG00000063244, Low tissue specificity
MIMi191318, gene
neXtProtiNX_P26368
OpenTargetsiENSG00000063244
PharmGKBiPA134908683

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG0120, Eukaryota
GeneTreeiENSGT00940000155556
InParanoidiP26368
OMAiRRMTQWD
OrthoDBi896650at2759
PhylomeDBiP26368
TreeFamiTF314111

Enzyme and pathway databases

PathwayCommonsiP26368
ReactomeiR-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72163, mRNA Splicing - Major Pathway
R-HSA-72187, mRNA 3'-end processing
R-HSA-73856, RNA Polymerase II Transcription Termination
SIGNORiP26368

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		11338, 757 hits in 855 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		U2AF2, human
EvolutionaryTraceiP26368

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		U2AF2

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		11338
PharosiP26368, Tbio

Protein Ontology

More...PROi		PR:P26368
RNActiP26368, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000063244, Expressed in right testis and 232 other tissues
ExpressionAtlasiP26368, baseline and differential
GenevisibleiP26368, HS

Family and domain databases

Gene3Di3.30.70.330, 3 hits
IDEALiIID00147
InterProiView protein in InterPro
IPR012677, Nucleotide-bd_a/b_plait_sf
IPR035979, RBD_domain_sf
IPR000504, RRM_dom
IPR006529, U2AF_lg
PfamiView protein in Pfam
PF00076, RRM_1, 3 hits
SMARTiView protein in SMART
SM00360, RRM, 3 hits
SUPFAMiSSF54928, SSF54928, 3 hits
TIGRFAMsiTIGR01642, U2AF_lg, 1 hit
PROSITEiView protein in PROSITE
PS50102, RRM, 3 hits

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiU2AF2_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P26368
Secondary accession number(s): Q96HC5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: December 2, 2020
This is version 226 of the entry and version 4 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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