UniProtKB - P26368 (U2AF2_HUMAN)
Splicing factor U2AF 65 kDa subunit
U2AF2
Functioni
GO - Molecular functioni
- C2H2 zinc finger domain binding Source: Ensembl
- enzyme binding Source: UniProtKB
- poly-pyrimidine tract binding Source: GO_Central
- pre-mRNA 3'-splice site binding Source: GO_Central
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA 3'-end processing Source: Reactome
- mRNA export from nucleus Source: Reactome
- mRNA processing Source: ProtInc
- mRNA splicing, via spliceosome Source: GO_Central
- negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
- negative regulation of protein ubiquitination Source: BHF-UCL
- positive regulation of RNA splicing Source: UniProtKB
- RNA export from nucleus Source: Reactome
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | P26368 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination |
SIGNORi | P26368 |
Names & Taxonomyi
Protein namesi | Recommended name: Splicing factor U2AF 65 kDa subunitAlternative name(s): U2 auxiliary factor 65 kDa subunit Short name: hU2AF(65) Short name: hU2AF65 U2 snRNP auxiliary factor large subunit |
Gene namesi | Name:U2AF2 Synonyms:U2AF65 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000063244.12 |
HGNCi | HGNC:23156, U2AF2 |
MIMi | 191318, gene |
neXtProti | NX_P26368 |
Subcellular locationi
Nucleus
Nucleus
- commitment complex Source: GO_Central
- nuclear speck Source: GO_Central
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
- spliceosomal complex Source: HGNC-UCL
- U2-type prespliceosome Source: GO_Central
- U2AF complex Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 92 | W → A: Decreases affinity for UAF1 by 3 orders of magnitude. 1 Publication | 1 | |
Mutagenesisi | 96 | P → G: Decreases affinity for UAF1 by 2 orders of magnitude. 1 Publication | 1 | |
Mutagenesisi | 104 | P → G: Decreases affinity for UAF1 by 2 orders of magnitude. 1 Publication | 1 | |
Mutagenesisi | 387 – 388 | EE → RR: Reduces interaction with SF1. 1 Publication | 2 | |
Mutagenesisi | 391 – 394 | DDEE → AAAA: Reduces interaction with SF1. | 4 | |
Mutagenesisi | 391 – 394 | DDEE → RRKK: Reduces interaction with SF1. | 4 | |
Mutagenesisi | 396 – 397 | EE → AA: No effect. 1 Publication | 2 | |
Mutagenesisi | 396 – 397 | EE → GA: Reduces interaction with SF1. 1 Publication | 2 | |
Mutagenesisi | 396 – 397 | EE → KK: Reduces interaction with SF1. 1 Publication | 2 | |
Mutagenesisi | 454 | F → A: Reduces interaction with SF1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 11338 |
OpenTargetsi | ENSG00000063244 |
PharmGKBi | PA134908683 |
Miscellaneous databases
Pharosi | P26368, Tbio |
Polymorphism and mutation databases
BioMutai | U2AF2 |
DMDMi | 267188 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000081988 | 2 – 475 | Splicing factor U2AF 65 kDa subunitAdd BLAST | 474 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 2 | PhosphoserineCombined sources | 1 | |
Modified residuei | 15 | 5-hydroxylysine; by JMJD6; alternate1 Publication | 1 | |
Cross-linki | 15 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 70 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 70 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 79 | PhosphoserineCombined sources | 1 | |
Modified residuei | 276 | 5-hydroxylysine; by JMJD61 Publication | 1 | |
Modified residuei | 294 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P26368 |
jPOSTi | P26368 |
MassIVEi | P26368 |
MaxQBi | P26368 |
PaxDbi | P26368 |
PeptideAtlasi | P26368 |
PRIDEi | P26368 |
ProteomicsDBi | 54325 [P26368-1] 54326 [P26368-2] |
TopDownProteomicsi | P26368-1 [P26368-1] P26368-2 [P26368-2] |
PTM databases
iPTMneti | P26368 |
PhosphoSitePlusi | P26368 |
SwissPalmi | P26368 |
Expressioni
Gene expression databases
Bgeei | ENSG00000063244, Expressed in right testis and 232 other tissues |
ExpressionAtlasi | P26368, baseline and differential |
Genevisiblei | P26368, HS |
Organism-specific databases
HPAi | ENSG00000063244, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with U2AF1L4 (By similarity). Heterodimer with U2AF1 (PubMed:11551507). Binds unphosphorylated SF1 (PubMed:10449420, PubMed:12718882).
Interacts with SCAF11 and SNW1 (PubMed:9447963, PubMed:21460037).
Interacts with ZRSR2/U2AF1-RS2.
Interacts with RBM17 (PubMed:17589525).
Interacts with PRPF19; the interaction is direct.
Interacts with POLR2A (via the C-terminal domain); recruits PRPF19 and the Prp19 complex to the pre-mRNA (PubMed:21536736).
Interacts with KHDC4 (Isoform 2) (PubMed:19641227).
Interacts with ZRSR2 (PubMed:9237760).
Interacts with the SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A (PubMed:27720643).
Interacts (via N-terminus) with CPSF7 (via C-terminus); this interaction stimulates pre-mRNA 3'-end processing by promoting the recruitment of the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).
By similarity12 PublicationsBinary interactionsi
Hide detailsP26368
Isoform 2 [P26368-2]
GO - Molecular functioni
- C2H2 zinc finger domain binding Source: Ensembl
- enzyme binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116466, 435 interactors |
ComplexPortali | CPX-1074, SF1-U2AF65 splicing factor complex |
CORUMi | P26368 |
DIPi | DIP-2154N |
IntActi | P26368, 126 interactors |
MINTi | P26368 |
STRINGi | 9606.ENSP00000307863 |
Miscellaneous databases
RNActi | P26368, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P26368 |
SMRi | P26368 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26368 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 149 – 231 | RRM 1PROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 259 – 337 | RRM 2PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 385 – 466 | RRM 3PROSITE-ProRule annotationAdd BLAST | 82 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 93 | Required for interaction with PRPF191 PublicationAdd BLAST | 92 | |
Regioni | 17 – 47 | Necessary and sufficient to stimulate pre-mRNAs 3'-end cleavage in a CFIm complex-dependent manner1 PublicationAdd BLAST | 31 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 27 – 62 | Arg/Ser-rich (RS domain)Add BLAST | 36 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0120, Eukaryota |
GeneTreei | ENSGT00940000155556 |
InParanoidi | P26368 |
OMAi | RRMTQWD |
OrthoDBi | 896650at2759 |
PhylomeDBi | P26368 |
TreeFami | TF314111 |
Family and domain databases
Gene3Di | 3.30.70.330, 3 hits |
IDEALi | IID00147 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR006529, U2AF_lg |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
TIGRFAMsi | TIGR01642, U2AF_lg, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSDFDEFERQ LNENKQERDK ENRHRKRSHS RSRSRDRKRR SRSRDRRNRD
60 70 80 90 100
QRSASRDRRR RSKPLTRGAK EEHGGLIRSP RHEKKKKVRK YWDVPPPGFE
110 120 130 140 150
HITPMQYKAM QAAGQIPATA LLPTMTPDGL AVTPTPVPVV GSQMTRQARR
160 170 180 190 200
LYVGNIPFGI TEEAMMDFFN AQMRLGGLTQ APGNPVLAVQ INQDKNFAFL
210 220 230 240 250
EFRSVDETTQ AMAFDGIIFQ GQSLKIRRPH DYQPLPGMSE NPSVYVPGVV
260 270 280 290 300
STVVPDSAHK LFIGGLPNYL NDDQVKELLT SFGPLKAFNL VKDSATGLSK
310 320 330 340 350
GYAFCEYVDI NVTDQAIAGL NGMQLGDKKL LVQRASVGAK NATLVSPPST
360 370 380 390 400
INQTPVTLQV PGLMSSQVQM GGHPTEVLCL MNMVLPEELL DDEEYEEIVE
410 420 430 440 450
DVRDECSKYG LVKSIEIPRP VDGVEVPGCG KIFVEFTSVF DCQKAMQGLT
460 470
GRKFANRVVV TKYCDPDSYH RRDFW
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7ENG2 | K7ENG2_HUMAN | U2 snRNP auxiliary factor large sub... | U2AF2 | 307 | Annotation score: | ||
M0QYQ9 | M0QYQ9_HUMAN | Splicing factor U2AF 65 kDa subunit | U2AF2 | 51 | Annotation score: | ||
K7ELP8 | K7ELP8_HUMAN | Splicing factor U2AF 65 kDa subunit | U2AF2 | 86 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_035414 | 345 – 348 | Missing in isoform 2. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64044 mRNA Translation: CAA45409.1 CH471135 Genomic DNA Translation: EAW72404.1 BC008740 mRNA Translation: AAH08740.1 BC030574 mRNA Translation: AAH30574.1 |
CCDSi | CCDS12933.1 [P26368-1] CCDS46197.1 [P26368-2] |
PIRi | S20250 |
RefSeqi | NP_001012496.1, NM_001012478.1 [P26368-2] NP_009210.1, NM_007279.2 [P26368-1] |
Genome annotation databases
Ensembli | ENST00000308924; ENSP00000307863; ENSG00000063244 [P26368-1] ENST00000450554; ENSP00000388475; ENSG00000063244 [P26368-2] |
GeneIDi | 11338 |
KEGGi | hsa:11338 |
UCSCi | uc002qlt.4, human [P26368-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64044 mRNA Translation: CAA45409.1 CH471135 Genomic DNA Translation: EAW72404.1 BC008740 mRNA Translation: AAH08740.1 BC030574 mRNA Translation: AAH30574.1 |
CCDSi | CCDS12933.1 [P26368-1] CCDS46197.1 [P26368-2] |
PIRi | S20250 |
RefSeqi | NP_001012496.1, NM_001012478.1 [P26368-2] NP_009210.1, NM_007279.2 [P26368-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JMT | X-ray | 2.20 | B | 85-112 | [»] | |
1O0P | NMR | - | A | 372-475 | [»] | |
1OPI | NMR | - | A | 372-475 | [»] | |
1U2F | NMR | - | A | 148-237 | [»] | |
2G4B | X-ray | 2.50 | A | 148-336 | [»] | |
2HZC | X-ray | 1.47 | A | 148-229 | [»] | |
2M0G | NMR | - | B | 372-475 | [»] | |
2U2F | NMR | - | A | 258-342 | [»] | |
2YH0 | NMR | - | A | 148-342 | [»] | |
2YH1 | NMR | - | A | 148-342 | [»] | |
3VAF | X-ray | 2.49 | A/B | 148-336 | [»] | |
3VAG | X-ray | 2.19 | A/B | 148-336 | [»] | |
3VAH | X-ray | 2.50 | A/B | 148-336 | [»] | |
3VAI | X-ray | 2.20 | A/B | 148-336 | [»] | |
3VAJ | X-ray | 1.90 | A/B | 148-336 | [»] | |
3VAK | X-ray | 2.17 | A/B | 148-336 | [»] | |
3VAL | X-ray | 2.50 | A/B/D/I | 148-336 | [»] | |
3VAM | X-ray | 2.40 | A/B | 148-336 | [»] | |
4FXW | X-ray | 2.29 | A/C | 375-475 | [»] | |
4TU7 | X-ray | 2.09 | A/B | 148-336 | [»] | |
4TU8 | X-ray | 1.92 | A/B | 148-336 | [»] | |
4TU9 | X-ray | 1.99 | A/B | 148-336 | [»] | |
5EV1 | X-ray | 2.04 | A | 141-341 | [»] | |
5EV2 | X-ray | 1.86 | A | 141-341 | [»] | |
5EV3 | X-ray | 1.50 | A | 141-341 | [»] | |
5EV4 | X-ray | 1.57 | A | 141-341 | [»] | |
5W0G | X-ray | 1.07 | A | 148-229 | [»] | |
5W0H | X-ray | 1.11 | A | 258-336 | [»] | |
6TR0 | NMR | - | A | 140-342 | [»] | |
BMRBi | P26368 | |||||
SMRi | P26368 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116466, 435 interactors |
ComplexPortali | CPX-1074, SF1-U2AF65 splicing factor complex |
CORUMi | P26368 |
DIPi | DIP-2154N |
IntActi | P26368, 126 interactors |
MINTi | P26368 |
STRINGi | 9606.ENSP00000307863 |
PTM databases
iPTMneti | P26368 |
PhosphoSitePlusi | P26368 |
SwissPalmi | P26368 |
Polymorphism and mutation databases
BioMutai | U2AF2 |
DMDMi | 267188 |
Proteomic databases
EPDi | P26368 |
jPOSTi | P26368 |
MassIVEi | P26368 |
MaxQBi | P26368 |
PaxDbi | P26368 |
PeptideAtlasi | P26368 |
PRIDEi | P26368 |
ProteomicsDBi | 54325 [P26368-1] 54326 [P26368-2] |
TopDownProteomicsi | P26368-1 [P26368-1] P26368-2 [P26368-2] |
Protocols and materials databases
Antibodypediai | 4399, 247 antibodies |
DNASUi | 11338 |
Genome annotation databases
Ensembli | ENST00000308924; ENSP00000307863; ENSG00000063244 [P26368-1] ENST00000450554; ENSP00000388475; ENSG00000063244 [P26368-2] |
GeneIDi | 11338 |
KEGGi | hsa:11338 |
UCSCi | uc002qlt.4, human [P26368-1] |
Organism-specific databases
CTDi | 11338 |
DisGeNETi | 11338 |
EuPathDBi | HostDB:ENSG00000063244.12 |
GeneCardsi | U2AF2 |
HGNCi | HGNC:23156, U2AF2 |
HPAi | ENSG00000063244, Low tissue specificity |
MIMi | 191318, gene |
neXtProti | NX_P26368 |
OpenTargetsi | ENSG00000063244 |
PharmGKBi | PA134908683 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0120, Eukaryota |
GeneTreei | ENSGT00940000155556 |
InParanoidi | P26368 |
OMAi | RRMTQWD |
OrthoDBi | 896650at2759 |
PhylomeDBi | P26368 |
TreeFami | TF314111 |
Enzyme and pathway databases
PathwayCommonsi | P26368 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination |
SIGNORi | P26368 |
Miscellaneous databases
BioGRID-ORCSi | 11338, 757 hits in 855 CRISPR screens |
ChiTaRSi | U2AF2, human |
EvolutionaryTracei | P26368 |
GeneWikii | U2AF2 |
GenomeRNAii | 11338 |
Pharosi | P26368, Tbio |
PROi | PR:P26368 |
RNActi | P26368, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000063244, Expressed in right testis and 232 other tissues |
ExpressionAtlasi | P26368, baseline and differential |
Genevisiblei | P26368, HS |
Family and domain databases
Gene3Di | 3.30.70.330, 3 hits |
IDEALi | IID00147 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR006529, U2AF_lg |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
TIGRFAMsi | TIGR01642, U2AF_lg, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | U2AF2_HUMAN | |
Accessioni | P26368Primary (citable) accession number: P26368 Secondary accession number(s): Q96HC5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 226 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM