UniProtKB - P26324 (VSPF1_CALRH)
Thrombin-like enzyme ancrod
Functioni
Thrombin-like snake venom serine protease that acts as an anticoagulant. It cleaves fibrinogen (FGA) to split off the A-fibrinopeptides (A, AY and AP), but not the B-fibrinopeptide. The resulting fibrin polymers are imperfectly formed and much smaller in size (1 to 2 um long) than the fibrin polymers produced by the action of thrombin. These ancrod-induced microthrombi are friable, unstable, urea-soluble and have significantly degraded alpha chains. They do not cross-link to form thrombi. They are markedly susceptible to digestion by plasmin and are rapidly removed from circulation by either reticuloendothelial phagocytosis or normal fibrinolysis, or both. Anticoagulation through the removal of fibrinogen from the blood is rapid, occurring within hours following its administration. It does not activate plasminogen and does not degrade preformed, fully cross-linked thrombin fibrin. It also reduces the level of plasminogen activator inhibitor (PAI) and may stimulate the release of tissue plasminogen activator (PLAT) from the endothelium. The profibrinolytic effect of these 2 actions appears to be limited to local microthrombus degradation.
Catalytic activityi
- Selective cleavage of Arg-|-Xaa bond in fibrinogen, to form fibrin, and release fibrinopeptide A. The specificity of further degradation of fibrinogen varies with species origin of the enzyme. EC:3.4.21.74
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 43 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 88 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 182 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Blood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin |
Enzyme and pathway databases
BRENDAi | 3.4.21.74, 198 |
Protein family/group databases
MEROPSi | S01.178 |
Names & Taxonomyi
Protein namesi | Recommended name: Thrombin-like enzyme ancrod (EC:3.4.21.74)Short name: SVTLE Alternative name(s): Fibrinogen-clotting enzyme Snake venom serine protease Short name: SVSP Venombin A |
Organismi | Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma) |
Taxonomic identifieri | 8717 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Crotalinae › Calloselasma |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Pharmaceutical usei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088730 | 1 – 234 | Thrombin-like enzyme ancrodAdd BLAST | 234 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 7 ↔ 141 | PROSITE-ProRule annotation | ||
Glycosylationi | 23 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 28 ↔ 44 | PROSITE-ProRule annotation | ||
Disulfide bondi | 78 ↔ 232 | PROSITE-ProRule annotation | ||
Glycosylationi | 79 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 99 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 120 ↔ 188 | PROSITE-ProRule annotation | ||
Glycosylationi | 148 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 152 ↔ 167 | PROSITE-ProRule annotation | ||
Disulfide bondi | 178 ↔ 203 | PROSITE-ProRule annotation | ||
Glycosylationi | 229 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinPTM databases
GlyConnecti | 50, 24 N-Linked glycans |
iPTMneti | P26324 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Monomer.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 227 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 227 |
Sequence similaritiesi
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
VIGGDECNIN EHRFLVAVYE GTNWTFICGG VLIHPEWVIT AEHCARRRMN
60 70 80 90 100
LVFGMHRKSE KFDDEQERYP KKRYFIRCNK TRTSWDEDIM LIRLNKPVNN
110 120 130 140 150
SEHIAPLSLP SNPPIVGSDC RVMGWGSINR RIDVLSDEPR CANINLHNFT
160 170 180 190 200
MCHGLFRKMP KKGRVLCAGD LRGRRDSCNS DSGGPLICNE ELHGIVARGP
210 220 230
NPCAQPNKPA LYTSIYDYRD WVNNVIAGNA TCSP
Sequence databases
PIRi | S20407 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Ancrod entry |
RxMed Viprinex entry |
Sequence databases
PIRi | S20407 |
3D structure databases
AlphaFoldDBi | P26324 |
SMRi | P26324 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | S01.178 |
PTM databases
GlyConnecti | 50, 24 N-Linked glycans |
iPTMneti | P26324 |
Enzyme and pathway databases
BRENDAi | 3.4.21.74, 198 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VSPF1_CALRH | |
Accessioni | P26324Primary (citable) accession number: P26324 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, PharmaceuticalDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families