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UniProtKB - P26297 (LDHD_LACDA)
Protein
D-lactate dehydrogenase
Gene
ldhA
Organism
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14)
Status
Functioni
Catalytic activityi
- EC:1.1.1.28
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 176 | NADCombined sources1 Publication | 1 | |
Binding sitei | 213 | NADCombined sources1 Publication | 1 | |
Active sitei | 236 | 1 Publication | 1 | |
Binding sitei | 260 | NADCombined sources1 Publication | 1 | |
Active sitei | 265 | 1 Publication | 1 | |
Active sitei | 297 | Proton donor1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 156 – 157 | NADCombined sources1 Publication | 2 | |
Nucleotide bindingi | 207 – 208 | NAD1 Publication | 2 | |
Nucleotide bindingi | 234 – 236 | NAD1 Publication | 3 |
GO - Molecular functioni
- D-lactate dehydrogenase activity Source: UniProtKB-EC
- NAD binding Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.1.1.28, 2853 |
SABIO-RKi | P26297 |
Names & Taxonomyi
Protein namesi | Recommended name: D-lactate dehydrogenase (EC:1.1.1.28)Short name: D-LDH Alternative name(s): D-specific 2-hydroxyacid dehydrogenase |
Gene namesi | Name:ldhA Ordered Locus Names:Ldb0101 |
Organismi | Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) |
Taxonomic identifieri | 390333 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 206 | H → Q: Increase of activity. 1 Publication | 1 | |
Mutagenesisi | 236 | R → K: Decrease of activity. 1 Publication | 1 | |
Mutagenesisi | 260 | D → N: Decrease of activity. 1 Publication | 1 | |
Mutagenesisi | 265 | E → Q: Decrease of activity. 1 Publication | 1 | |
Mutagenesisi | 297 | H → Q: 90% loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000075953 | 2 – 333 | D-lactate dehydrogenaseAdd BLAST | 332 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 390333.Ldb0101 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P26297 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26297 |
Family & Domainsi
Sequence similaritiesi
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1052, Bacteria |
HOGENOMi | CLU_019796_1_1_9 |
OMAi | VIVTAHQ |
Family and domain databases
InterProi | View protein in InterPro IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065, D_2_HYDROXYACID_DH_1, 1 hit PS00670, D_2_HYDROXYACID_DH_2, 1 hit PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P26297-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKIFAYAIR EDEKPFLKEW EDAHKDVEVE YTDKLLTPET VALAKGADGV
60 70 80 90 100
VVYQQLDYTA ETLQALADNG ITKMSLRNVG VDNIDMAKAK ELGFQITNVP
110 120 130 140 150
VYSPNAIAEH AAIQAARILR QDKAMDEKVA RHDLRWAPTI GREVRDQVVG
160 170 180 190 200
VIGTGHIGQV FMQIMEGFGA KVIAYDIFRN PELEKKGYYV DSLDDLYKQA
210 220 230 240 250
DVISLHVPDV PANVHMINDE SIAKMKQDVV IVNVSRGPLV DTDAVIRGLD
260 270 280 290 300
SGKIFGYAMD VYEGEVGIFN EDWEGKEFPD ARLADLIARP NVLVTPHTAF
310 320 330
YTTHAVRNMV VKAFDNNLEL VEGKEAETPV KVG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | V → A in AAA25246 (PubMed:1569100).Curated | 1 | |
Sequence conflicti | 117 | R → A in CAA42781 (PubMed:1915894).Curated | 1 | |
Sequence conflicti | 122 | D → A in AAA25246 (PubMed:1569100).Curated | 1 | |
Sequence conflicti | 152 | I → V in AAA25246 (PubMed:1569100).Curated | 1 | |
Sequence conflicti | 174 | A → T in CAA42781 (PubMed:1915894).Curated | 1 | |
Sequence conflicti | 220 | E → K in AAA25246 (PubMed:1569100).Curated | 1 | |
Sequence conflicti | 254 | I → V in AAA25246 (PubMed:1569100).Curated | 1 | |
Sequence conflicti | 268 | I → V in AAA25246 (PubMed:1569100).Curated | 1 | |
Sequence conflicti | 273 | W → R in AAA25246 (PubMed:1569100).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60220 Genomic DNA Translation: CAA42781.1 M85224 Genomic DNA Translation: AAA25246.1 CR954253 Genomic DNA Translation: CAI96942.1 |
PIRi | A38094 |
RefSeqi | WP_011543503.1, NZ_JQAV01000045.1 |
Genome annotation databases
EnsemblBacteriai | CAI96942; CAI96942; Ldb0101 |
KEGGi | ldb:Ldb0101 |
PATRICi | fig|390333.13.peg.1571 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60220 Genomic DNA Translation: CAA42781.1 M85224 Genomic DNA Translation: AAA25246.1 CR954253 Genomic DNA Translation: CAI96942.1 |
PIRi | A38094 |
RefSeqi | WP_011543503.1, NZ_JQAV01000045.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1J49 | X-ray | 2.20 | A/B | 1-333 | [»] | |
1J4A | X-ray | 1.90 | A/B/C/D | 1-333 | [»] | |
SMRi | P26297 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 390333.Ldb0101 |
Genome annotation databases
EnsemblBacteriai | CAI96942; CAI96942; Ldb0101 |
KEGGi | ldb:Ldb0101 |
PATRICi | fig|390333.13.peg.1571 |
Phylogenomic databases
eggNOGi | COG1052, Bacteria |
HOGENOMi | CLU_019796_1_1_9 |
OMAi | VIVTAHQ |
Enzyme and pathway databases
BRENDAi | 1.1.1.28, 2853 |
SABIO-RKi | P26297 |
Miscellaneous databases
EvolutionaryTracei | P26297 |
Family and domain databases
InterProi | View protein in InterPro IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065, D_2_HYDROXYACID_DH_1, 1 hit PS00670, D_2_HYDROXYACID_DH_2, 1 hit PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LDHD_LACDA | |
Accessioni | P26297Primary (citable) accession number: P26297 Secondary accession number(s): Q1G7V7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families