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Protein

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

Gene

folK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

This enzyme is heat stable.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Dihydroneopterin aldolase (folB)
  4. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (folK)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
Biological processFolate biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER
MetaCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.7.6.3 2026

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
P26281

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00077;UER00155

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
Alternative name(s):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Short name:
PPPK
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
HPPK
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:folK
Ordered Locus Names:b0142, JW0138
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG11374 folK

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...
ChEMBLi
CHEMBL3217379

Drug and drug target database

More...
DrugBanki
DB03197 6-Hydroxymethylpterin
DB04047 [Pterin-6-Yl Methanyl]-Phosphonophosphate

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved2 Publications
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001682492 – 1592-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinaseAdd BLAST158

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P26281

PRoteomics IDEntifications database

More...
PRIDEi
P26281

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
P26281, 2 interactors

STRING: functional protein association networks

More...
STRINGi
316385.ECDH10B_0122

Chemistry databases

BindingDB database of measured binding affinities

More...
BindingDBi
P26281

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1159
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P26281

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P26281

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P26281

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the HPPK family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105K8U Bacteria
COG0801 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000217741

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P26281

KEGG Orthology (KO)

More...
KOi
K00950

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P26281

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00483 HPPK, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.70.560, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000550 Hppk
IPR035907 Hppk_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01288 HPPK, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55083 SSF55083, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01498 folK, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00794 HPPK, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P26281-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTVAYIAIGS NLASPLEQVN AALKALGDIP ESHILTVSSF YRTPPLGPQD
60 70 80 90 100
QPDYLNAAVA LETSLAPEEL LNHTQRIELQ QGRVRKAERW GPRTLDLDIM
110 120 130 140 150
LFGNEVINTE RLTVPHYDMK NRGFMLWPLF EIAPELVFPD GEMLRQILHT

RAFDKLNKW
Length:159
Mass (Da):18,079
Last modified:January 23, 2007 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i67301CD634D0A174
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
L06495 Genomic DNA Translation: AAB53446.1
M20574 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC73253.1
AP009048 Genomic DNA Translation: BAB96719.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A43325

NCBI Reference Sequences

More...
RefSeqi
NP_414684.1, NC_000913.3
WP_000215139.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC73253; AAC73253; b0142
BAB96719; BAB96719; BAB96719

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
948792

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW0138
eco:b0142

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2139

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06495 Genomic DNA Translation: AAB53446.1
M20574 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC73253.1
AP009048 Genomic DNA Translation: BAB96719.1
PIRiA43325
RefSeqiNP_414684.1, NC_000913.3
WP_000215139.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DY3X-ray2.00A2-159[»]
1EQ0NMR-A2-159[»]
1EQMX-ray1.50A2-159[»]
1EX8X-ray1.85A2-159[»]
1F9HX-ray1.50A2-159[»]
1G4CX-ray1.65A/B2-159[»]
1HKAX-ray1.50A2-159[»]
1HQ2X-ray1.25A2-159[»]
1IM6X-ray1.74A2-159[»]
1KBRX-ray1.55A2-159[»]
1Q0NX-ray1.25A2-159[»]
1RAOX-ray1.56A2-159[»]
1RB0X-ray1.35A2-159[»]
1RTZX-ray1.33A2-159[»]
1RU1X-ray1.40A/B2-159[»]
1RU2X-ray1.48A2-159[»]
1TMJX-ray1.45A2-159[»]
1TMMX-ray1.25A/B2-159[»]
2F63NMR-A2-159[»]
2F65NMR-A2-159[»]
3HCXX-ray1.75A2-159[»]
3HD1X-ray1.30A2-159[»]
3HD2X-ray1.10A2-159[»]
3HSDX-ray1.65A/B2-159[»]
3HSGX-ray1.14A2-159[»]
3HSJX-ray1.18A2-159[»]
3HSZX-ray1.40A2-159[»]
3HT0X-ray1.40A2-159[»]
3ILIX-ray1.45A2-159[»]
3ILJX-ray1.65A2-159[»]
3ILLX-ray1.73A2-159[»]
3ILOX-ray1.10A2-159[»]
3IP0X-ray0.89A2-159[»]
3KUEX-ray1.54A2-159[»]
3KUGX-ray2.00A2-159[»]
3KUHX-ray1.35A2-159[»]
3UD5X-ray2.00A2-159[»]
3UDEX-ray1.88A2-159[»]
3UDVX-ray1.88A2-159[»]
4F7VX-ray1.73A2-159[»]
4M5GX-ray1.31A1-159[»]
4M5HX-ray1.11A1-159[»]
4M5IX-ray1.08A1-159[»]
4M5JX-ray1.70A1-159[»]
4M5KX-ray1.30A1-159[»]
4M5LX-ray1.09A1-159[»]
4M5MX-ray1.12A1-159[»]
4M5NX-ray2.00A/B1-159[»]
5ETKX-ray1.09A1-159[»]
5ETLX-ray1.82A/B/C/D1-159[»]
5ETMX-ray1.46A1-159[»]
5ETNX-ray1.40A1-159[»]
5ETOX-ray1.07A1-159[»]
5ETPX-ray1.05A1-159[»]
6AN4X-ray1.47A2-159[»]
6AN6X-ray2.30A/B2-159[»]
ProteinModelPortaliP26281
SMRiP26281
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP26281, 2 interactors
STRINGi316385.ECDH10B_0122

Chemistry databases

BindingDBiP26281
ChEMBLiCHEMBL3217379
DrugBankiDB03197 6-Hydroxymethylpterin
DB04047 [Pterin-6-Yl Methanyl]-Phosphonophosphate

Proteomic databases

PaxDbiP26281
PRIDEiP26281

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73253; AAC73253; b0142
BAB96719; BAB96719; BAB96719
GeneIDi948792
KEGGiecj:JW0138
eco:b0142
PATRICifig|1411691.4.peg.2139

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1348
EcoGeneiEG11374 folK

Phylogenomic databases

eggNOGiENOG4105K8U Bacteria
COG0801 LUCA
HOGENOMiHOG000217741
InParanoidiP26281
KOiK00950
PhylomeDBiP26281

Enzyme and pathway databases

UniPathwayi
UPA00077;UER00155

BioCyciEcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER
MetaCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER
BRENDAi2.7.6.3 2026
SABIO-RKiP26281

Miscellaneous databases

EvolutionaryTraceiP26281

Protein Ontology

More...
PROi
PR:P26281

Family and domain databases

CDDicd00483 HPPK, 1 hit
Gene3Di3.30.70.560, 1 hit
InterProiView protein in InterPro
IPR000550 Hppk
IPR035907 Hppk_sf
PfamiView protein in Pfam
PF01288 HPPK, 1 hit
SUPFAMiSSF55083 SSF55083, 1 hit
TIGRFAMsiTIGR01498 folK, 1 hit
PROSITEiView protein in PROSITE
PS00794 HPPK, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHPPK_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P26281
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: December 5, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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