folK

Functionⁱ

Miscellaneous

This enzyme is heat stable.

Catalytic activityⁱ

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.

Pathwayⁱ: tetrahydrofolate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

no protein annotated in this organism
no protein annotated in this organism
Dihydroneopterin aldolase (folB)
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (folK)

This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functionⁱ

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 1325970
ATP binding Source: UniProtKB-KW
kinase activity Source: UniProtKB-KW
magnesium ion binding
Source: EcoCyc
Inferred from direct assayⁱ
- 10825540

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

folic acid biosynthetic process Source: UniProtKB-KW
tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Biological process	Folate biosynthesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER MetaCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER
BRENDAⁱ	2.7.6.3 2026
SABIO-RKⁱ	P26281
UniPathwayⁱ	UPA00077; UER00155

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3) Alternative name(s): 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase Short name: PPPK 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase Short name: HPPK
Gene namesⁱ	Name:folK Ordered Locus Names:b0142, JW0138
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11374 folK

EcoGeneⁱ

EG11374 folK

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL3217379
Drug and drug target database More...DrugBankⁱ	DB03197 6-Hydroxymethylpterin DB04047 [Pterin-6-Yl Methanyl]-Phosphonophosphate

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed2 Publications
ChainⁱPRO_0000168249	2 – 159	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinaseAdd BLAST		158

Proteomic databases

PaxDbⁱ	P26281
PRIDEⁱ	P26281

Interactionⁱ

Subunit structureⁱ

Monomer.

Protein-protein interaction databases

IntActⁱ	P26281, 2 interactors
STRINGⁱ	316385.ECDH10B_0122

Chemistry databases

BindingDBⁱ

P26281

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	3 – 10	Combined sources	8
Beta strandⁱ	12 – 14	Combined sources	3
Helixⁱ	15 – 27	Combined sources	13
Beta strandⁱ	32 – 37	Combined sources	6
Beta strandⁱ	41 – 43	Combined sources	3
Beta strandⁱ	46 – 48	Combined sources	3
Beta strandⁱ	49 – 51	Combined sources	3
Beta strandⁱ	54 – 63	Combined sources	10
Helixⁱ	67 – 80	Combined sources	14
Turnⁱ	82 – 86	Combined sources	5
Beta strandⁱ	87 – 90	Combined sources	4
Helixⁱ	91 – 93	Combined sources	3
Beta strandⁱ	96 – 102	Combined sources	7
Beta strandⁱ	107 – 111	Combined sources	5
Beta strandⁱ	113 – 115	Combined sources	3
Helixⁱ	119 – 121	Combined sources	3
Helixⁱ	123 – 132	Combined sources	10
Beta strandⁱ	139 – 141	Combined sources	3
Helixⁱ	144 – 151	Combined sources	8

3D structure databases

ProteinModelPortalⁱ	P26281
SMRⁱ	P26281
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P26281

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the HPPK family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105K8U Bacteria COG0801 LUCA
HOGENOMⁱ	HOG000217741
InParanoidⁱ	P26281
KEGG Orthology (KO) More...KOⁱ	K00950
OMAⁱ	KDERWGP
PhylomeDBⁱ	P26281

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00483 HPPK, 1 hit
Gene3Dⁱ	3.30.70.560, 1 hit
InterProⁱ	View protein in InterPro IPR000550 Hppk IPR035907 Hppk_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01288 HPPK, 1 hit
SUPFAMⁱ	SSF55083 SSF55083, 1 hit
TIGRFAMsⁱ	TIGR01498 folK, 1 hit
PROSITEⁱ	View protein in PROSITE PS00794 HPPK, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P26281-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTVAYIAIGS NLASPLEQVN AALKALGDIP ESHILTVSSF YRTPPLGPQD 
        60         70         80         90        100
QPDYLNAAVA LETSLAPEEL LNHTQRIELQ QGRVRKAERW GPRTLDLDIM 
       110        120        130        140        150
LFGNEVINTE RLTVPHYDMK NRGFMLWPLF EIAPELVFPD GEMLRQILHT 

RAFDKLNKW

Length:159

Mass (Da):18,079

Last modified:January 23, 2007 - v3

Checksum:ⁱ67301CD634D0A174

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L06495 Genomic DNA Translation: AAB53446.1 M20574 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73253.1 AP009048 Genomic DNA Translation: BAB96719.1
PIRⁱ	A43325
NCBI Reference Sequences More...RefSeqⁱ	NP_414684.1, NC_000913.3 WP_000215139.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73253; AAC73253; b0142 BAB96719; BAB96719; BAB96719
GeneIDⁱ	948792
KEGGⁱ	ecj:JW0138 eco:b0142
PATRICⁱ	fig\|1411691.4.peg.2139

Protein	Similar proteins		Species	Score	Length
P26281	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		ECOLX		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		Escherichia coli 1-110-08_S3_C1		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		Escherichia coli 2-210-07_S3_C3		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase		Escherichia coli DEC6A		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase		SHISO		159
+13

Protein	Similar proteins		Species	Score	Length
P26281	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		ECOLX		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		Escherichia coli 1-110-08_S3_C1		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		Escherichia coli 2-210-07_S3_C3		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase		Escherichia coli DEC6A		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase		SHISO		159
+225

Protein	Similar proteins		Species	Score	Length
P26281	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		ECOLX		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		Escherichia coli 1-110-08_S3_C1		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase		Escherichia coli 2-210-07_S3_C3		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase		Escherichia coli DEC6A		159
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase		SHISO		159
+1665

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L06495 Genomic DNA Translation: AAB53446.1 M20574 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73253.1 AP009048 Genomic DNA Translation: BAB96719.1
PIRⁱ	A43325
NCBI Reference Sequences More...RefSeqⁱ	NP_414684.1, NC_000913.3 WP_000215139.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1DY3	X-ray	2.00	A	2-159	[»]
	1EQ0	NMR	-	A	2-159	[»]
	1EQM	X-ray	1.50	A	2-159	[»]
	1EX8	X-ray	1.85	A	2-159	[»]
	1F9H	X-ray	1.50	A	2-159	[»]
	1G4C	X-ray	1.65	A/B	2-159	[»]
	1HKA	X-ray	1.50	A	2-159	[»]
	1HQ2	X-ray	1.25	A	2-159	[»]
	1IM6	X-ray	1.74	A	2-159	[»]
	1KBR	X-ray	1.55	A	2-159	[»]
	1Q0N	X-ray	1.25	A	2-159	[»]
	1RAO	X-ray	1.56	A	2-159	[»]
	1RB0	X-ray	1.35	A	2-159	[»]
	1RTZ	X-ray	1.33	A	2-159	[»]
	1RU1	X-ray	1.40	A/B	2-159	[»]
	1RU2	X-ray	1.48	A	2-159	[»]
	1TMJ	X-ray	1.45	A	2-159	[»]
	1TMM	X-ray	1.25	A/B	2-159	[»]
	2F63	NMR	-	A	2-159	[»]
	2F65	NMR	-	A	2-159	[»]
	3HCX	X-ray	1.75	A	2-159	[»]
	3HD1	X-ray	1.30	A	2-159	[»]
	3HD2	X-ray	1.10	A	2-159	[»]
	3HSD	X-ray	1.65	A/B	2-159	[»]
	3HSG	X-ray	1.14	A	2-159	[»]
	3HSJ	X-ray	1.18	A	2-159	[»]
	3HSZ	X-ray	1.40	A	2-159	[»]
	3HT0	X-ray	1.40	A	2-159	[»]
	3ILI	X-ray	1.45	A	2-159	[»]
	3ILJ	X-ray	1.65	A	2-159	[»]
	3ILL	X-ray	1.73	A	2-159	[»]
	3ILO	X-ray	1.10	A	2-159	[»]
	3IP0	X-ray	0.89	A	2-159	[»]
	3KUE	X-ray	1.54	A	2-159	[»]
	3KUG	X-ray	2.00	A	2-159	[»]
	3KUH	X-ray	1.35	A	2-159	[»]
	3UD5	X-ray	2.00	A	2-159	[»]
	3UDE	X-ray	1.88	A	2-159	[»]
	3UDV	X-ray	1.88	A	2-159	[»]
	4F7V	X-ray	1.73	A	2-159	[»]
	4M5G	X-ray	1.31	A	1-159	[»]
	4M5H	X-ray	1.11	A	1-159	[»]
	4M5I	X-ray	1.08	A	1-159	[»]
	4M5J	X-ray	1.70	A	1-159	[»]
	4M5K	X-ray	1.30	A	1-159	[»]
	4M5L	X-ray	1.09	A	1-159	[»]
	4M5M	X-ray	1.12	A	1-159	[»]
	4M5N	X-ray	2.00	A/B	1-159	[»]
	5ETK	X-ray	1.09	A	1-159	[»]
	5ETL	X-ray	1.82	A/B/C/D	1-159	[»]
	5ETM	X-ray	1.46	A	1-159	[»]
	5ETN	X-ray	1.40	A	1-159	[»]
	5ETO	X-ray	1.07	A	1-159	[»]
	5ETP	X-ray	1.05	A	1-159	[»]
ProteinModelPortalⁱ	P26281
SMRⁱ	P26281
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P26281, 2 interactors
STRINGⁱ	316385.ECDH10B_0122

Chemistry databases

BindingDBⁱ	P26281
ChEMBLⁱ	CHEMBL3217379
Drug and drug target database More...DrugBankⁱ	DB03197 6-Hydroxymethylpterin DB04047 [Pterin-6-Yl Methanyl]-Phosphonophosphate

Proteomic databases

PaxDbⁱ	P26281
PRIDEⁱ	P26281

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73253; AAC73253; b0142 BAB96719; BAB96719; BAB96719
GeneIDⁱ	948792
KEGGⁱ	ecj:JW0138 eco:b0142
PATRICⁱ	fig\|1411691.4.peg.2139

Organism-specific databases

EchoBASEⁱ	EB1348
EcoGeneⁱ	EG11374 folK

Phylogenomic databases

eggNOGⁱ	ENOG4105K8U Bacteria COG0801 LUCA
HOGENOMⁱ	HOG000217741
InParanoidⁱ	P26281
KEGG Orthology (KO) More...KOⁱ	K00950
OMAⁱ	KDERWGP
PhylomeDBⁱ	P26281

Enzyme and pathway databases

UniPathwayⁱ	UPA00077; UER00155
BioCycⁱ	EcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER MetaCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER
BRENDAⁱ	2.7.6.3 2026
SABIO-RKⁱ	P26281

Miscellaneous databases

EvolutionaryTraceⁱ	P26281
Protein Ontology More...PROⁱ	PR:P26281

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00483 HPPK, 1 hit
Gene3Dⁱ	3.30.70.560, 1 hit
InterProⁱ	View protein in InterPro IPR000550 Hppk IPR035907 Hppk_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01288 HPPK, 1 hit
SUPFAMⁱ	SSF55083 SSF55083, 1 hit
TIGRFAMsⁱ	TIGR01498 folK, 1 hit
PROSITEⁱ	View protein in PROSITE PS00794 HPPK, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HPPK_ECOLI
Accessionⁱ	P26281Primary (citable) accession number: P26281
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
	Last sequence update:	January 23, 2007
	Last modified:	May 23, 2018
	This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

UniProtKB

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Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P26281 (HPPK_ECOLI)

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2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tetrahydrofolate biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: tetrahydrofolate biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ