We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - P26281 (HPPK_ECOLI)
Protein
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
Gene
folK
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of pyrophosphate from adenosine triphosphate (ATP) to 6-hydroxymethyl-7,8-dihydropterin, an enzymatic step in folate biosynthesis pathway.
1 PublicationCatalytic activityi
- 6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H+1 PublicationEC:2.7.6.31 PublicationThis reaction proceeds in the forward1 Publication direction.
Temperature dependencei
Thermostable. Retains 75% of its activity after heating 30 minutes at 96 degrees Celsius.1 Publication
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.1 Publication This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
GO - Molecular functioni
- 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: EcoliWiki
- ATP binding Source: UniProtKB-KW
- kinase activity Source: UniProtKB-KW
- magnesium ion binding Source: EcoCyc
GO - Biological processi
- folic acid biosynthetic process Source: UniProtKB-KW
- tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Folate biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER |
BRENDAi | 2.7.6.3, 2026 |
SABIO-RKi | P26281 |
UniPathwayi | UPA00077;UER00155 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.31 Publication)Alternative name(s): 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase Short name: PPPK 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase Short name: HPPK |
Gene namesi | Name:folK Ordered Locus Names:b0142, JW0138 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL3217379 |
DrugBanki | DB04158, 6-(adenosine tetraphosphate-methyl)-7,8-dihydropterin DB02119, 6-Hydroxymethyl-7,8-Dihydropterin DB03197, 6-Hydroxymethylpterin DB04047, 6-hydroxymethylpterin diphosphate DB04610, 7,8-dihydro-6-hydroxymethyl-7-methyl-7-[2-phenylethyl]-pterin DB02596, alpha,beta-Methyleneadenosine 5'-triphosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000168249 | 2 – 159 | 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinaseAdd BLAST | 158 |
Proteomic databases
jPOSTi | P26281 |
PaxDbi | P26281 |
PRIDEi | P26281 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
IntActi | P26281, 2 interactors |
STRINGi | 511145.b0142 |
Chemistry databases
BindingDBi | P26281 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P26281 |
BMRBi | P26281 |
SMRi | P26281 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26281 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HPPK family.Curated
Phylogenomic databases
eggNOGi | COG0801, Bacteria |
HOGENOMi | CLU_097916_0_1_6 |
InParanoidi | P26281 |
OMAi | QVILRQN |
PhylomeDBi | P26281 |
Family and domain databases
CDDi | cd00483, HPPK, 1 hit |
Gene3Di | 3.30.70.560, 1 hit |
InterProi | View protein in InterPro IPR000550, Hppk IPR035907, Hppk_sf |
Pfami | View protein in Pfam PF01288, HPPK, 1 hit |
SUPFAMi | SSF55083, SSF55083, 1 hit |
TIGRFAMsi | TIGR01498, folK, 1 hit |
PROSITEi | View protein in PROSITE PS00794, HPPK, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P26281-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVAYIAIGS NLASPLEQVN AALKALGDIP ESHILTVSSF YRTPPLGPQD
60 70 80 90 100
QPDYLNAAVA LETSLAPEEL LNHTQRIELQ QGRVRKAERW GPRTLDLDIM
110 120 130 140 150
LFGNEVINTE RLTVPHYDMK NRGFMLWPLF EIAPELVFPD GEMLRQILHT
RAFDKLNKW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06495 Genomic DNA Translation: AAB53446.1 M20574 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73253.1 AP009048 Genomic DNA Translation: BAB96719.1 |
PIRi | A43325 |
RefSeqi | NP_414684.1, NC_000913.3 WP_000215139.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73253; AAC73253; b0142 BAB96719; BAB96719; BAB96719 |
GeneIDi | 948792 |
KEGGi | ecj:JW0138 eco:b0142 |
PATRICi | fig|1411691.4.peg.2139 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06495 Genomic DNA Translation: AAB53446.1 M20574 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73253.1 AP009048 Genomic DNA Translation: BAB96719.1 |
PIRi | A43325 |
RefSeqi | NP_414684.1, NC_000913.3 WP_000215139.1, NZ_STEB01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DY3 | X-ray | 2.00 | A | 2-159 | [»] | |
1EQ0 | NMR | - | A | 2-159 | [»] | |
1EQM | X-ray | 1.50 | A | 2-159 | [»] | |
1EX8 | X-ray | 1.85 | A | 2-159 | [»] | |
1F9H | X-ray | 1.50 | A | 2-159 | [»] | |
1G4C | X-ray | 1.65 | A/B | 2-159 | [»] | |
1HKA | X-ray | 1.50 | A | 2-159 | [»] | |
1HQ2 | X-ray | 1.25 | A | 2-159 | [»] | |
1IM6 | X-ray | 1.74 | A | 2-159 | [»] | |
1KBR | X-ray | 1.55 | A | 2-159 | [»] | |
1Q0N | X-ray | 1.25 | A | 2-159 | [»] | |
1RAO | X-ray | 1.56 | A | 2-159 | [»] | |
1RB0 | X-ray | 1.35 | A | 2-159 | [»] | |
1RTZ | X-ray | 1.33 | A | 2-159 | [»] | |
1RU1 | X-ray | 1.40 | A/B | 2-159 | [»] | |
1RU2 | X-ray | 1.48 | A | 2-159 | [»] | |
1TMJ | X-ray | 1.45 | A | 2-159 | [»] | |
1TMM | X-ray | 1.25 | A/B | 2-159 | [»] | |
2F63 | NMR | - | A | 2-159 | [»] | |
2F65 | NMR | - | A | 2-159 | [»] | |
3HCX | X-ray | 1.75 | A | 2-159 | [»] | |
3HD1 | X-ray | 1.30 | A | 2-159 | [»] | |
3HD2 | X-ray | 1.10 | A | 2-159 | [»] | |
3HSD | X-ray | 1.65 | A/B | 2-159 | [»] | |
3HSG | X-ray | 1.14 | A | 2-159 | [»] | |
3HSJ | X-ray | 1.18 | A | 2-159 | [»] | |
3HSZ | X-ray | 1.40 | A | 2-159 | [»] | |
3HT0 | X-ray | 1.40 | A | 2-159 | [»] | |
3ILI | X-ray | 1.45 | A | 2-159 | [»] | |
3ILJ | X-ray | 1.65 | A | 2-159 | [»] | |
3ILL | X-ray | 1.73 | A | 2-159 | [»] | |
3ILO | X-ray | 1.10 | A | 2-159 | [»] | |
3IP0 | X-ray | 0.89 | A | 2-159 | [»] | |
3KUE | X-ray | 1.54 | A | 2-159 | [»] | |
3KUG | X-ray | 2.00 | A | 2-159 | [»] | |
3KUH | X-ray | 1.35 | A | 2-159 | [»] | |
3UD5 | X-ray | 2.00 | A | 2-159 | [»] | |
3UDE | X-ray | 1.88 | A | 2-159 | [»] | |
3UDV | X-ray | 1.88 | A | 2-159 | [»] | |
4F7V | X-ray | 1.73 | A | 2-159 | [»] | |
4M5G | X-ray | 1.31 | A | 1-159 | [»] | |
4M5H | X-ray | 1.11 | A | 1-159 | [»] | |
4M5I | X-ray | 1.08 | A | 1-159 | [»] | |
4M5J | X-ray | 1.70 | A | 1-159 | [»] | |
4M5K | X-ray | 1.30 | A | 1-159 | [»] | |
4M5L | X-ray | 1.09 | A | 1-159 | [»] | |
4M5M | X-ray | 1.12 | A | 1-159 | [»] | |
4M5N | X-ray | 2.00 | A/B | 1-159 | [»] | |
5ETK | X-ray | 1.09 | A | 1-159 | [»] | |
5ETL | X-ray | 1.82 | A/B/C/D | 1-159 | [»] | |
5ETM | X-ray | 1.46 | A | 1-159 | [»] | |
5ETN | X-ray | 1.40 | A | 1-159 | [»] | |
5ETO | X-ray | 1.07 | A | 1-159 | [»] | |
5ETP | X-ray | 1.05 | A | 1-159 | [»] | |
6AN4 | X-ray | 1.47 | A | 2-159 | [»] | |
6AN6 | X-ray | 2.30 | A/B | 2-159 | [»] | |
7KDO | X-ray | 1.60 | A | 2-159 | [»] | |
7KDR | X-ray | 1.49 | A | 2-159 | [»] | |
AlphaFoldDBi | P26281 | |||||
BMRBi | P26281 | |||||
SMRi | P26281 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P26281, 2 interactors |
STRINGi | 511145.b0142 |
Chemistry databases
BindingDBi | P26281 |
ChEMBLi | CHEMBL3217379 |
DrugBanki | DB04158, 6-(adenosine tetraphosphate-methyl)-7,8-dihydropterin DB02119, 6-Hydroxymethyl-7,8-Dihydropterin DB03197, 6-Hydroxymethylpterin DB04047, 6-hydroxymethylpterin diphosphate DB04610, 7,8-dihydro-6-hydroxymethyl-7-methyl-7-[2-phenylethyl]-pterin DB02596, alpha,beta-Methyleneadenosine 5'-triphosphate |
Proteomic databases
jPOSTi | P26281 |
PaxDbi | P26281 |
PRIDEi | P26281 |
Genome annotation databases
EnsemblBacteriai | AAC73253; AAC73253; b0142 BAB96719; BAB96719; BAB96719 |
GeneIDi | 948792 |
KEGGi | ecj:JW0138 eco:b0142 |
PATRICi | fig|1411691.4.peg.2139 |
Organism-specific databases
EchoBASEi | EB1348 |
Phylogenomic databases
eggNOGi | COG0801, Bacteria |
HOGENOMi | CLU_097916_0_1_6 |
InParanoidi | P26281 |
OMAi | QVILRQN |
PhylomeDBi | P26281 |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00155 |
BioCyci | EcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER |
BRENDAi | 2.7.6.3, 2026 |
SABIO-RKi | P26281 |
Miscellaneous databases
EvolutionaryTracei | P26281 |
PROi | PR:P26281 |
Family and domain databases
CDDi | cd00483, HPPK, 1 hit |
Gene3Di | 3.30.70.560, 1 hit |
InterProi | View protein in InterPro IPR000550, Hppk IPR035907, Hppk_sf |
Pfami | View protein in Pfam PF01288, HPPK, 1 hit |
SUPFAMi | SSF55083, SSF55083, 1 hit |
TIGRFAMsi | TIGR01498, folK, 1 hit |
PROSITEi | View protein in PROSITE PS00794, HPPK, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HPPK_ECOLI | |
Accessioni | P26281Primary (citable) accession number: P26281 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 181 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families