UniProtKB - P26265 (CPDB_SALTY)
Protein
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase
Gene
cpdB
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'-cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate.
Miscellaneous
Two kinetically distinguishable active sites for the two substrates (2',3'-cyclic nucleotides and 3'-nucleotides) have been identified.
Catalytic activityi
Cofactori
a divalent metal cationBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 31 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 33 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 76 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 76 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 116 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 225 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 257 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 259 | Divalent metal cation 1By similarity | 1 | |
Binding sitei | 440 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity Source: UniProtKB-EC
- 3'-nucleotidase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- nucleotide catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme |
Ligand | Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:cpdB Ordered Locus Names:STM4403 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- outer membrane-bounded periplasmic space Source: GO_Central
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000000036 | 20 – 647 | 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidaseAdd BLAST | 628 |
Proteomic databases
PaxDbi | P26265 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 544 – 550 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Belongs to the 5'-nucleotidase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
HOGENOMi | CLU_005854_4_1_6 |
OMAi | EKAQYPM |
PhylomeDBi | P26265 |
Family and domain databases
CDDi | cd07410, MPP_CpdB_N, 1 hit |
Gene3Di | 3.60.21.10, 1 hit 3.90.780.10, 1 hit |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR041827, CpdB_N IPR006294, Cyc_nuc_PDE_nucleotidase IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 1 hit |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 1 hit PF00149, Metallophos, 1 hit |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 1 hit |
TIGRFAMsi | TIGR01390, CycNucDiestase, 1 hit |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 1 hit PS00786, 5_NUCLEOTIDASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P26265-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKFSATLLA TLIAASVNAA TVDLRIMETT DLHSNMMDFD YYKDTATEKF
60 70 80 90 100
GLVRTASLIH AARNEVKNSV LVDNGDLIQG SPLGDYMAAK GLKDGDVHPV
110 120 130 140 150
YKALNTLDYA VGNLGNHEFN YGLDYLHNAL AGAKFPYVNA NIIDVKTQKP
160 170 180 190 200
LFTPYLIKET SVIDKDGNPQ TLKIGYIGFV PPQIMIWDKA NLSGKVTVND
210 220 230 240 250
ITETARKYVP EMREKGADIV VVIAHSGLSA DPYHSMAENS VYYLSEVPGV
260 270 280 290 300
DAIMFGHAHA VFPGKDFADI KGADIAKGTL NGIPAVMPGM WGDHLGVVDL
310 320 330 340 350
VLNNDSGKWQ VTQAKAEARP IYDAAAKKSL AAEDSKLVGI LKADHDATRE
360 370 380 390 400
FVSKPIGKSA DNMYSYLALV QDDPTVQVVN NAQKAYVEHF IQGDPDLAKL
410 420 430 440 450
PVLSAAAPFK VGGRKNDPAS FVEVEKGQLT FRNAADLYLY PNTLVVVKAS
460 470 480 490 500
GKEVKEWLEC SAGQFNQIDI HSNKPQSLIN WDGFRTYNFD VIDGVNYQID
510 520 530 540 550
VSQPARYDGE CQMVNPQAER IKNLTFNGKP VDPNATFLVA TNNYRAYGGK
560 570 580 590 600
FAGTGDSHIA FASPDENRAV LAAWIGAESK RAGEIHPAAD NNWRLAPIHS
610 620 630 640
DTALDIRFET SPGDKAAAFI KAKGQYPMKK VAVDDIGFAI YQVDLSK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | G → A in CAA37956 (PubMed:2172762).Curated | 1 | |
Sequence conflicti | 84 – 89 | GDYMAA → RLYGG in CAA37956 (PubMed:2172762).Curated | 6 | |
Sequence conflicti | 96 – 98 | DVH → GIQ in CAA37956 (PubMed:2172762).Curated | 3 | |
Sequence conflicti | 133 | A → G in CAA37956 (PubMed:2172762).Curated | 1 | |
Sequence conflicti | 174 | I → N in CAA37956 (PubMed:2172762).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006468 Genomic DNA Translation: AAL23223.1 X54009 Genomic DNA Translation: CAA37956.1 |
PIRi | S11915 |
RefSeqi | NP_463264.1, NC_003197.2 WP_000589395.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL23223; AAL23223; STM4403 |
GeneIDi | 1255929 |
KEGGi | stm:STM4403 |
PATRICi | fig|99287.12.peg.4628 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006468 Genomic DNA Translation: AAL23223.1 X54009 Genomic DNA Translation: CAA37956.1 |
PIRi | S11915 |
RefSeqi | NP_463264.1, NC_003197.2 WP_000589395.1, NC_003197.2 |
3D structure databases
SMRi | P26265 |
ModBasei | Search... |
Proteomic databases
PaxDbi | P26265 |
Genome annotation databases
EnsemblBacteriai | AAL23223; AAL23223; STM4403 |
GeneIDi | 1255929 |
KEGGi | stm:STM4403 |
PATRICi | fig|99287.12.peg.4628 |
Phylogenomic databases
HOGENOMi | CLU_005854_4_1_6 |
OMAi | EKAQYPM |
PhylomeDBi | P26265 |
Family and domain databases
CDDi | cd07410, MPP_CpdB_N, 1 hit |
Gene3Di | 3.60.21.10, 1 hit 3.90.780.10, 1 hit |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR041827, CpdB_N IPR006294, Cyc_nuc_PDE_nucleotidase IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 1 hit |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 1 hit PF00149, Metallophos, 1 hit |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 1 hit |
TIGRFAMsi | TIGR01390, CycNucDiestase, 1 hit |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 1 hit PS00786, 5_NUCLEOTIDASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CPDB_SALTY | |
Accessioni | P26265Primary (citable) accession number: P26265 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | December 13, 2001 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families