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UniProtKB - P26225 (GUNB_CELFI)
Protein
Endoglucanase B
Gene
cenB
Organism
Cellulomonas fimi
Status
Functioni
The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 410 | PROSITE-ProRule annotation | 1 | |
Active sitei | 449 | PROSITE-ProRule annotation | 1 | |
Active sitei | 458 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cellulase activity Source: UniProtKB-EC
- cellulose binding Source: InterPro
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Protein family/group databases
CAZyi | CBM2, Carbohydrate-Binding Module Family 2 CBM3, Carbohydrate-Binding Module Family 3 GH9, Glycoside Hydrolase Family 9 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase B (EC:3.2.1.4)Alternative name(s): Cellulase B Endo-1,4-beta-glucanase B |
Gene namesi | Name:cenB |
Organismi | Cellulomonas fimi |
Taxonomic identifieri | 1708 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Micrococcales › Cellulomonadaceae › Cellulomonas |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 33 | Sequence analysisAdd BLAST | 33 | |
ChainiPRO_0000007945 | 34 – 1045 | Endoglucanase BAdd BLAST | 1012 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 946 ↔ 1044 | By similarity |
Keywords - PTMi
Disulfide bondFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 493 – 642 | CBM3PROSITE-ProRule annotationAdd BLAST | 150 | |
Domaini | 653 – 743 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 751 – 840 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 849 – 940 | Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST | 92 | |
Domaini | 939 – 1045 | CBM2PROSITE-ProRule annotationAdd BLAST | 107 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 492 | CatalyticAdd BLAST | 459 | |
Regioni | 644 – 650 | Linker ('hinge') (Pro-Thr box) | 7 | |
Regioni | 734 – 748 | Linker ('hinge') (Pro-Thr box)Add BLAST | 15 | |
Regioni | 831 – 846 | Linker ('hinge') (Pro-Thr box)Add BLAST | 16 | |
Regioni | 931 – 944 | Linker ('hinge') (Pro-Thr box)Add BLAST | 14 |
Domaini
The linker region (also termed 'hinge') may be a potential site for proteolysis.1 Publication
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalPhylogenomic databases
OMAi | RWLDYWT |
Family and domain databases
CDDi | cd00063, FN3, 3 hits |
Gene3Di | 1.50.10.10, 1 hit 2.60.40.10, 3 hits 2.60.40.290, 1 hit 2.60.40.710, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001919, CBD2 IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR001956, CBM3 IPR036966, CBM3_sf IPR003961, FN3_dom IPR036116, FN3_sf IPR001701, Glyco_hydro_9 IPR033126, Glyco_hydro_9_Asp/Glu_AS IPR018221, Glyco_hydro_9_His_AS IPR013783, Ig-like_fold |
Pfami | View protein in Pfam PF00553, CBM_2, 1 hit PF00942, CBM_3, 1 hit PF00041, fn3, 3 hits PF00759, Glyco_hydro_9, 1 hit |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit SM01067, CBM_3, 1 hit SM00060, FN3, 3 hits |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF49265, SSF49265, 2 hits SSF49384, SSF49384, 2 hits |
PROSITEi | View protein in PROSITE PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS51172, CBM3, 1 hit PS50853, FN3, 3 hits PS60032, GH9_1, 1 hit PS00592, GH9_2, 1 hit PS00698, GH9_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P26225-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRQVPRTLV AGGSALAVAV GVLVAPLATG AAAAPTYNYA EALQKSMFFY
60 70 80 90 100
QAQRSGDLPA DFPVSWRGDS GLTDGADVGK DLTGGWYDAG DHVKFGFPMA
110 120 130 140 150
FSATMLAWGA IESPTGYSKA GSLDELKDNL RFVSDYFVKA HTAPNELYVQ
160 170 180 190 200
VGDGEADHKW WGPAEVMTMA RPSHKISASC PGSDVAAETA AALASSAIVL
210 220 230 240 250
KGDDPAYAAT LVSHAKQLYT FADTYRGAYS DCVTAASAYY KSWSGYQDEL
260 270 280 290 300
VWGAYWLYKA TGDATYLAKA EAEYDKLGTE NQSTTRSYKW TIAWDNKQFG
310 320 330 340 350
TYALLAMETG KQKYVDDANR WLDYWTVGVN GQKVPYSPGG QAVLDSWGAL
360 370 380 390 400
RYAANTSFVA LVYSDWMTDA TRKARYHDFG VRQINYALGD NPRSSSYVVG
410 420 430 440 450
FGANPPTAPH HRTAHGSWLD SITTPAQSRH VLYGALVGGP GSPNDAYTDS
460 470 480 490 500
RQDYVANEVA TDYNAGFTSA LARLVEEYGG TPLASFPTPE QPDGDQLFVE
510 520 530 540 550
AMLNQPPSGT FTEVKAMIRN QSAFPARSLK NAKVRYWFTT DGFAASDVTL
560 570 580 590 600
SANYSECGAQ SGKGVSAGGT LGYVELSCVG QDIHPGGQSQ HRREIQFRLT
610 620 630 640 650
GPAGWNPAND PSYTGLTQTA LAKASAITLY DGSTLVWGKE PTGTTTDTTP
660 670 680 690 700
PTTPGTPVAT GVTTVGASLS WAASTDAGSG VAGYELYRVQ GTTQTLVGTT
710 720 730 740 750
TAAAYILRDL TPGTAYSYVV KAKDVAGNVS AASAAVTFTT DTTGETEPPT
760 770 780 790 800
TPGTPVASAV TSTGATLAWA PSTGDPAVSG YDVLRVQGTT TTVVAQTTVP
810 820 830 840 850
TVTLSGLTPS TAYTYAVRAK NVAGDVSALS APVTFTTAAP PVDTVAPTVP
860 870 880 890 900
GTPVASNVAT TGATLTWTAS TDSGGSGLAG YEVLRVSGTT QTLVASPTTA
910 920 930 940 950
TVALAGLTPA TAYSYVVRAK DGAGNVSAVS SPVTFTTLPV TSTPSCTVVY
960 970 980 990 1000
STNSWNVGFT GSVKITNTGT TPLTWTLGFA FPSGQQVTQG WSATWSQTGT
1010 1020 1030 1040
TVTATGLSWN ATLQPGQSTD IGFNGSHPGT NTNPASFTVN GEVCG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64644 Genomic DNA Translation: AAA23086.1 |
PIRi | A39199 |
RefSeqi | WP_013769201.1, NZ_LR134387.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64644 Genomic DNA Translation: AAA23086.1 |
PIRi | A39199 |
RefSeqi | WP_013769201.1, NZ_LR134387.1 |
3D structure databases
AlphaFoldDBi | P26225 |
SMRi | P26225 |
ModBasei | Search... |
Protein family/group databases
CAZyi | CBM2, Carbohydrate-Binding Module Family 2 CBM3, Carbohydrate-Binding Module Family 3 GH9, Glycoside Hydrolase Family 9 |
Phylogenomic databases
OMAi | RWLDYWT |
Family and domain databases
CDDi | cd00063, FN3, 3 hits |
Gene3Di | 1.50.10.10, 1 hit 2.60.40.10, 3 hits 2.60.40.290, 1 hit 2.60.40.710, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001919, CBD2 IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR001956, CBM3 IPR036966, CBM3_sf IPR003961, FN3_dom IPR036116, FN3_sf IPR001701, Glyco_hydro_9 IPR033126, Glyco_hydro_9_Asp/Glu_AS IPR018221, Glyco_hydro_9_His_AS IPR013783, Ig-like_fold |
Pfami | View protein in Pfam PF00553, CBM_2, 1 hit PF00942, CBM_3, 1 hit PF00041, fn3, 3 hits PF00759, Glyco_hydro_9, 1 hit |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit SM01067, CBM_3, 1 hit SM00060, FN3, 3 hits |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF49265, SSF49265, 2 hits SSF49384, SSF49384, 2 hits |
PROSITEi | View protein in PROSITE PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS51172, CBM3, 1 hit PS50853, FN3, 3 hits PS60032, GH9_1, 1 hit PS00592, GH9_2, 1 hit PS00698, GH9_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GUNB_CELFI | |
Accessioni | P26225Primary (citable) accession number: P26225 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families