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Entry version 153 (02 Dec 2020)
Sequence version 2 (23 Jan 2002)
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Protein

Regulatory protein ada

Gene

ada

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei34DNABy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei38Nucleophile; methyl group acceptor from phosphotriesterPROSITE-ProRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi38ZincBy similarity1
Metal bindingi42ZincBy similarity1
Binding sitei43DNABy similarity1
Binding sitei67DNABy similarity1
Metal bindingi69ZincBy similarity1
Metal bindingi72ZincBy similarity1
Active sitei321Nucleophile; methyl group acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi103 – 124H-T-H motifPROSITE-ProRule annotationAdd BLAST22
DNA bindingi150 – 173H-T-H motifPROSITE-ProRule annotationAdd BLAST24

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding, Methyltransferase, Transferase
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Regulatory protein ada
Alternative name(s):
Regulatory protein of adaptive response
Cleaved into the following chain:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63By similarity)
Alternative name(s):
O-6-methylguanine-DNA alkyltransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ada
Ordered Locus Names:STM2265
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri99287 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001014 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000187491 – 353Regulatory protein adaAdd BLAST353
ChainiPRO_0000018750179 – 353Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST175

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei128 – 129CleavageBy similarity2
Sitei178 – 179CleavageBy similarity2

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P26189

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P26189

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini94 – 183HTH araC/xylS-typePROSITE-ProRule annotationAdd BLAST90

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the C-terminal section; belongs to the MGMT family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_000445_52_0_6

Identification of Orthologs from Complete Genome Data

More...
OMAi
RFAIGQC

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P26189

Family and domain databases

Conserved Domains Database

More...
CDDi
cd06445, ATase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.10.10, 1 hit
3.40.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR035451, Ada-like_dom_sf
IPR004026, Ada_DNA_repair_Zn-bd
IPR016221, Bifunct_regulatory_prot_Ada
IPR009057, Homeobox-like_sf
IPR018060, HTH_AraC
IPR018062, HTH_AraC-typ_CS
IPR001497, MethylDNA_cys_MeTrfase_AS
IPR014048, MethylDNA_cys_MeTrfase_DNA-bd
IPR036217, MethylDNA_cys_MeTrfase_DNAb
IPR008332, MethylG_MeTrfase_N
IPR036631, MGMT_N_sf
IPR036388, WH-like_DNA-bd_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02805, Ada_Zn_binding, 1 hit
PF01035, DNA_binding_1, 1 hit
PF12833, HTH_18, 1 hit
PF02870, Methyltransf_1N, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000409, Ada, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00342, HTH_ARAC, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF46689, SSF46689, 1 hit
SSF46767, SSF46767, 1 hit
SSF53155, SSF53155, 1 hit
SSF57884, SSF57884, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00589, ogt, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00041, HTH_ARAC_FAMILY_1, 2 hits
PS01124, HTH_ARAC_FAMILY_2, 1 hit
PS00374, MGMT, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P26189-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMKKALLIDD ECWLRVQARD ASADGRFVFA VRTTGVFCRP SCRSKRALRK
60 70 80 90 100
NVRFFANAQQ ALDAGFRPCK RCQPDNARAQ QRRLDKIACA CRLLEQETPV
110 120 130 140 150
TLAFLAQAVA MSPFHLHRLF KASTGMTPKG WQQAWRARRL REALAKGEPI
160 170 180 190 200
TAAIYRAGFP DSSSYYRHAD QTLGMTAKQF RKGGDNVSVR YALTDWVYGR
210 220 230 240 250
CLVAESERGI CAILPGDSDD ALLAELHTLF PSARHEPADA LFQQRVRQVV
260 270 280 290 300
AAINTRDVLL SLPLDIQGTA FQQQVWQALC AIPCGETVSY QQLAATIGKP
310 320 330 340 350
TAVRAVASAC GANKLAMVIP CHRVVRRDGA LSGYRWGVRR KAQLLKREAQ

KEE
Length:353
Mass (Da):39,348
Last modified:January 23, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iACE9E13C7EF6C114
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAA14252 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D90221 Genomic DNA Translation: BAA14252.1 Different initiation.
AE006468 Genomic DNA Translation: AAL21167.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A39433, XYEBOT

NCBI Reference Sequences

More...
RefSeqi
NP_461208.1, NC_003197.2
WP_000975956.1, NC_003197.2

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAL21167; AAL21167; STM2265

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1253787

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
stm:STM2265

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|99287.12.peg.2399

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90221 Genomic DNA Translation: BAA14252.1 Different initiation.
AE006468 Genomic DNA Translation: AAL21167.1
PIRiA39433, XYEBOT
RefSeqiNP_461208.1, NC_003197.2
WP_000975956.1, NC_003197.2

3D structure databases

SMRiP26189
ModBaseiSearch...

Proteomic databases

PaxDbiP26189

Genome annotation databases

EnsemblBacteriaiAAL21167; AAL21167; STM2265
GeneIDi1253787
KEGGistm:STM2265
PATRICifig|99287.12.peg.2399

Phylogenomic databases

HOGENOMiCLU_000445_52_0_6
OMAiRFAIGQC
PhylomeDBiP26189

Family and domain databases

CDDicd06445, ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
3.40.10.10, 1 hit
InterProiView protein in InterPro
IPR035451, Ada-like_dom_sf
IPR004026, Ada_DNA_repair_Zn-bd
IPR016221, Bifunct_regulatory_prot_Ada
IPR009057, Homeobox-like_sf
IPR018060, HTH_AraC
IPR018062, HTH_AraC-typ_CS
IPR001497, MethylDNA_cys_MeTrfase_AS
IPR014048, MethylDNA_cys_MeTrfase_DNA-bd
IPR036217, MethylDNA_cys_MeTrfase_DNAb
IPR008332, MethylG_MeTrfase_N
IPR036631, MGMT_N_sf
IPR036388, WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF02805, Ada_Zn_binding, 1 hit
PF01035, DNA_binding_1, 1 hit
PF12833, HTH_18, 1 hit
PF02870, Methyltransf_1N, 1 hit
PIRSFiPIRSF000409, Ada, 1 hit
SMARTiView protein in SMART
SM00342, HTH_ARAC, 1 hit
SUPFAMiSSF46689, SSF46689, 1 hit
SSF46767, SSF46767, 1 hit
SSF53155, SSF53155, 1 hit
SSF57884, SSF57884, 1 hit
TIGRFAMsiTIGR00589, ogt, 1 hit
PROSITEiView protein in PROSITE
PS00041, HTH_ARAC_FAMILY_1, 2 hits
PS01124, HTH_ARAC_FAMILY_2, 1 hit
PS00374, MGMT, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiADA_SALTY
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P26189
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2002
Last modified: December 2, 2020
This is version 153 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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