UniProtKB - P26188 (MGMT_YEAST)
Protein
Methylated-DNA--protein-cysteine methyltransferase
Gene
MGT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Prefers double-stranded DNA over single-stranded DNA as substrate.3 Publications
Miscellaneous
Present with 150 molecules/cell in log phase YPD medium, but not detectable in stationary phase cells.1 Publication
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
Catalytic activityi
- a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]PROSITE-ProRule annotation2 PublicationsEC:2.1.1.63PROSITE-ProRule annotation2 Publications
- a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]PROSITE-ProRule annotation2 PublicationsEC:2.1.1.63PROSITE-ProRule annotation2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 120 | DNABy similarity | 1 | |
Binding sitei | 121 | DNA; via amide nitrogenBy similarity | 1 | |
Binding sitei | 134 | DNABy similarity | 1 | |
Active sitei | 151 | Nucleophile; methyl group acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 157 | DNA; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: SGD
GO - Biological processi
- DNA dealkylation involved in DNA repair Source: SGD
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Methyltransferase, Transferase |
Biological process | DNA damage, DNA repair |
Names & Taxonomyi
Protein namesi | Recommended name: Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.632 Publications)Alternative name(s): 6-O-methylguanine-DNA methyltransferase Short name: MGMT DNA repair MTase O-6-methylguanine-DNA-alkyltransferase |
Gene namesi | Name:MGT1 Ordered Locus Names:YDL200C ORF Names:D1204 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002359, MGT1 |
VEuPathDBi | FungiDB:YDL200C |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139362 | 1 – 188 | Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST | 188 |
Proteomic databases
PaxDbi | P26188 |
PRIDEi | P26188 |
Expressioni
Inductioni
In contrast to some bacterial and mammalian enzymes, MGT1 is not induced by alkylating agents.1 Publication
Interactioni
Binary interactionsi
Hide detailsP26188
With | #Exp. | IntAct |
---|---|---|
DUN1 [P39009] | 2 | EBI-10873,EBI-6194 |
UBR1 [P19812] | 2 | EBI-10873,EBI-19909 |
UFD4 [P33202] | 2 | EBI-10873,EBI-20010 |
Protein-protein interaction databases
BioGRIDi | 31846, 166 interactors |
DIPi | DIP-7461N |
IntActi | P26188, 50 interactors |
STRINGi | 4932.YDL200C |
Miscellaneous databases
RNActi | P26188, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the MGMT family.Curated
Phylogenomic databases
eggNOGi | KOG4062, Eukaryota |
GeneTreei | ENSGT00390000015799 |
HOGENOMi | CLU_000445_52_2_1 |
InParanoidi | P26188 |
OMAi | TFIETEV |
Family and domain databases
CDDi | cd06445, ATase, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR001497, MethylDNA_cys_MeTrfase_AS IPR014048, MethylDNA_cys_MeTrfase_DNA-bd IPR036217, MethylDNA_cys_MeTrfase_DNAb IPR008332, MethylG_MeTrfase_N IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF01035, DNA_binding_1, 1 hit PF02870, Methyltransf_1N, 1 hit |
SUPFAMi | SSF46767, SSF46767, 1 hit |
TIGRFAMsi | TIGR00589, ogt, 1 hit |
PROSITEi | View protein in PROSITE PS00374, MGMT, 1 hit |
i Sequence
Sequence statusi: Complete.
P26188-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKELLYYTFI ETEVTGAFLV FREKTQNLVF ASLGNDKLFL LGKVEGFLKK
60 70 80 90 100
HEKQDTMYDL QELKEAETYK KSIENYTICL ENKMPLPSGA IPFEFLFGTD
110 120 130 140 150
FQRKVWNELL NVEHGHVVTY GDIAKRIGKP TAARSVGRAC GSNNLALLVP
160 170 180
CHRIVGSNRK LTGYKWSCKL KEQLLNNEKE NSLSLSRL
Sequence cautioni
The sequence AAA34780 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA42920 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA67469 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA98777 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA98778 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60368 Genomic DNA Translation: CAA42920.1 Different initiation. M94227 Genomic DNA Translation: AAA34780.1 Different initiation. X99000 Genomic DNA Translation: CAA67469.1 Different initiation. Z74248 Genomic DNA Translation: CAA98778.1 Different initiation. Z74247 Genomic DNA Translation: CAA98777.1 Different initiation. X83276 Genomic DNA Translation: CAA58247.1 BK006938 Genomic DNA Translation: DAA11664.1 |
PIRi | S29370, XUBYMC |
RefSeqi | NP_010081.2, NM_001180260.1 |
Genome annotation databases
EnsemblFungii | YDL200C_mRNA; YDL200C; YDL200C |
GeneIDi | 851327 |
KEGGi | sce:YDL200C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60368 Genomic DNA Translation: CAA42920.1 Different initiation. M94227 Genomic DNA Translation: AAA34780.1 Different initiation. X99000 Genomic DNA Translation: CAA67469.1 Different initiation. Z74248 Genomic DNA Translation: CAA98778.1 Different initiation. Z74247 Genomic DNA Translation: CAA98777.1 Different initiation. X83276 Genomic DNA Translation: CAA58247.1 BK006938 Genomic DNA Translation: DAA11664.1 |
PIRi | S29370, XUBYMC |
RefSeqi | NP_010081.2, NM_001180260.1 |
3D structure databases
SMRi | P26188 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31846, 166 interactors |
DIPi | DIP-7461N |
IntActi | P26188, 50 interactors |
STRINGi | 4932.YDL200C |
Proteomic databases
PaxDbi | P26188 |
PRIDEi | P26188 |
Genome annotation databases
EnsemblFungii | YDL200C_mRNA; YDL200C; YDL200C |
GeneIDi | 851327 |
KEGGi | sce:YDL200C |
Organism-specific databases
SGDi | S000002359, MGT1 |
VEuPathDBi | FungiDB:YDL200C |
Phylogenomic databases
eggNOGi | KOG4062, Eukaryota |
GeneTreei | ENSGT00390000015799 |
HOGENOMi | CLU_000445_52_2_1 |
InParanoidi | P26188 |
OMAi | TFIETEV |
Miscellaneous databases
PROi | PR:P26188 |
RNActi | P26188, protein |
Family and domain databases
CDDi | cd06445, ATase, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR001497, MethylDNA_cys_MeTrfase_AS IPR014048, MethylDNA_cys_MeTrfase_DNA-bd IPR036217, MethylDNA_cys_MeTrfase_DNAb IPR008332, MethylG_MeTrfase_N IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF01035, DNA_binding_1, 1 hit PF02870, Methyltransf_1N, 1 hit |
SUPFAMi | SSF46767, SSF46767, 1 hit |
TIGRFAMsi | TIGR00589, ogt, 1 hit |
PROSITEi | View protein in PROSITE PS00374, MGMT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MGMT_YEAST | |
Accessioni | P26188Primary (citable) accession number: P26188 Secondary accession number(s): D6VRF4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 20, 2008 | |
Last modified: | February 10, 2021 | |
This is version 175 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - SIMILARITY comments
Index of protein domains and families