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UniProtKB - P26188 (MGMT_YEAST)

Entry version 175 (10 Feb 2021)
Sequence version 3 (20 May 2008)
Previous versions | rss
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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

MGT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Prefers double-stranded DNA over single-stranded DNA as substrate.3 Publications

Miscellaneous

Present with 150 molecules/cell in log phase YPD medium, but not detectable in stationary phase cells.1 Publication
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei120DNABy similarity1
Binding sitei121DNA; via amide nitrogenBy similarity1
Binding sitei134DNABy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei151Nucleophile; methyl group acceptorPROSITE-ProRule annotation1
Binding sitei157DNA; via amide nitrogenBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: SGD
Complete GO annotation on QuickGO ...

GO - Biological processi

  • DNA dealkylation involved in DNA repair Source: SGD
  • methylation Source: UniProtKB-KW
Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processDNA damage, DNA repair

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.632 Publications)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MGT1
Ordered Locus Names:YDL200C
ORF Names:D1204
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome IV

Organism-specific databases

Saccharomyces Genome Database

More...SGDi		S000002359, MGT1

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		FungiDB:YDL200C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001393621 – 188Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST188

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P26188

PRoteomics IDEntifications database

More...PRIDEi		P26188

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

In contrast to some bacterial and mammalian enzymes, MGT1 is not induced by alkylating agents.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		31846, 166 interactors

Database of interacting proteins

More...DIPi		DIP-7461N

Protein interaction database and analysis system

More...IntActi		P26188, 50 interactors

STRING: functional protein association networks

More...STRINGi		4932.YDL200C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P26188, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P26188

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG4062, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00390000015799

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_000445_52_2_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P26188

Identification of Orthologs from Complete Genome Data

More...OMAi		TFIETEV

Family and domain databases

Conserved Domains Database

More...CDDi		cd06445, ATase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001497, MethylDNA_cys_MeTrfase_AS
IPR014048, MethylDNA_cys_MeTrfase_DNA-bd
IPR036217, MethylDNA_cys_MeTrfase_DNAb
IPR008332, MethylG_MeTrfase_N
IPR036388, WH-like_DNA-bd_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01035, DNA_binding_1, 1 hit
PF02870, Methyltransf_1N, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF46767, SSF46767, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00589, ogt, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00374, MGMT, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P26188-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKELLYYTFI ETEVTGAFLV FREKTQNLVF ASLGNDKLFL LGKVEGFLKK 
        60         70         80         90        100
HEKQDTMYDL QELKEAETYK KSIENYTICL ENKMPLPSGA IPFEFLFGTD 
       110        120        130        140        150
FQRKVWNELL NVEHGHVVTY GDIAKRIGKP TAARSVGRAC GSNNLALLVP 
       160        170        180 
CHRIVGSNRK LTGYKWSCKL KEQLLNNEKE NSLSLSRL
Length:188
Mass (Da):21,499
Last modified:May 20, 2008 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i956E71B47016E47D
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA34780 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA42920 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA67469 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA98777 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA98778 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X60368 Genomic DNA Translation: CAA42920.1 Different initiation.
M94227 Genomic DNA Translation: AAA34780.1 Different initiation.
X99000 Genomic DNA Translation: CAA67469.1 Different initiation.
Z74248 Genomic DNA Translation: CAA98778.1 Different initiation.
Z74247 Genomic DNA Translation: CAA98777.1 Different initiation.
X83276 Genomic DNA Translation: CAA58247.1
BK006938 Genomic DNA Translation: DAA11664.1

Protein sequence database of the Protein Information Resource

More...PIRi		S29370, XUBYMC

NCBI Reference Sequences

More...RefSeqi		NP_010081.2, NM_001180260.1

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		YDL200C_mRNA; YDL200C; YDL200C

Database of genes from NCBI RefSeq genomes

More...GeneIDi		851327

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		sce:YDL200C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60368 Genomic DNA Translation: CAA42920.1 Different initiation.
M94227 Genomic DNA Translation: AAA34780.1 Different initiation.
X99000 Genomic DNA Translation: CAA67469.1 Different initiation.
Z74248 Genomic DNA Translation: CAA98778.1 Different initiation.
Z74247 Genomic DNA Translation: CAA98777.1 Different initiation.
X83276 Genomic DNA Translation: CAA58247.1
BK006938 Genomic DNA Translation: DAA11664.1
PIRiS29370, XUBYMC
RefSeqiNP_010081.2, NM_001180260.1

3D structure databases

SMRiP26188
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi31846, 166 interactors
DIPiDIP-7461N
IntActiP26188, 50 interactors
STRINGi4932.YDL200C

Proteomic databases

PaxDbiP26188
PRIDEiP26188

Genome annotation databases

EnsemblFungiiYDL200C_mRNA; YDL200C; YDL200C
GeneIDi851327
KEGGisce:YDL200C

Organism-specific databases

SGDiS000002359, MGT1
VEuPathDBiFungiDB:YDL200C

Phylogenomic databases

eggNOGiKOG4062, Eukaryota
GeneTreeiENSGT00390000015799
HOGENOMiCLU_000445_52_2_1
InParanoidiP26188
OMAiTFIETEV

Miscellaneous databases

Protein Ontology

More...PROi		PR:P26188
RNActiP26188, protein

Family and domain databases

CDDicd06445, ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001497, MethylDNA_cys_MeTrfase_AS
IPR014048, MethylDNA_cys_MeTrfase_DNA-bd
IPR036217, MethylDNA_cys_MeTrfase_DNAb
IPR008332, MethylG_MeTrfase_N
IPR036388, WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035, DNA_binding_1, 1 hit
PF02870, Methyltransf_1N, 1 hit
SUPFAMiSSF46767, SSF46767, 1 hit
TIGRFAMsiTIGR00589, ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374, MGMT, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMGMT_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P26188
Secondary accession number(s): D6VRF4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 20, 2008
Last modified: February 10, 2021
This is version 175 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
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