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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

Mgmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5ZincBy similarity1
Metal bindingi24ZincBy similarity1
Metal bindingi29ZincBy similarity1
Metal bindingi89ZincBy similarity1
Binding sitei99DNA; via amide nitrogenBy similarity1
Binding sitei118DNABy similarity1
Binding sitei119DNA; via amide nitrogenBy similarity1
Binding sitei127DNABy similarity1
Binding sitei132DNABy similarity1
Active sitei149Nucleophile; methyl group acceptorPROSITE-ProRule annotation1
Binding sitei155DNA; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:Mgmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96977 Mgmt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393601 – 211Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei205PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP26187
MaxQBiP26187
PaxDbiP26187
PeptideAtlasiP26187
PRIDEiP26187

PTM databases

iPTMnetiP26187
PhosphoSitePlusiP26187

Expressioni

Gene expression databases

BgeeiENSMUSG00000054612
CleanExiMM_MGMT
GenevisibleiP26187 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080224

Structurei

3D structure databases

ProteinModelPortaliP26187
SMRiP26187
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiKOG4062 Eukaryota
COG0350 LUCA
GeneTreeiENSGT00390000015799
HOGENOMiHOG000244137
HOVERGENiHBG001146
InParanoidiP26187
KOiK00567
OMAiMDKACEM
OrthoDBiEOG091G11SR
PhylomeDBiP26187
TreeFamiTF314064

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR008332 MethylG_MeTrfase_N
IPR036631 MGMT_N_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
PF02870 Methyltransf_1N, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
SSF53155 SSF53155, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

P26187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP
60 70 80 90 100
EVLGGPEGVP EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR
110 120 130 140 150
QVLWKLLKVV KFGETVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH
160 170 180 190 200
RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP TGQPASKGLG LTGTWLKSSF
210
ESTSSEPSGR N
Length:211
Mass (Da):22,435
Last modified:January 23, 2007 - v3
Checksum:i838BB92B9A1083D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84524 mRNA Translation: AAA37126.1
AB092489 mRNA Translation: BAC16763.1
AB092490 mRNA Translation: BAC16764.1
BC031888 mRNA Translation: AAH31888.1
CCDSiCCDS21945.1
PIRiA41809
RefSeqiNP_032624.1, NM_008598.2
XP_006507472.1, XM_006507409.3
XP_006507473.1, XM_006507410.3
UniGeneiMm.440219

Genome annotation databases

EnsembliENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612
GeneIDi17314
KEGGimmu:17314
UCSCiuc009keq.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMGMT_MOUSE
AccessioniPrimary (citable) accession number: P26187
Secondary accession number(s): Q54A49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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