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Protein

Anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase

Gene

bchE

Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the tetrapyrrole biosynthetic pathways leading to chlorophyll and bacteriochlorophyll (BChl). Catalyzes the anaerobic formation of the isocyclic ring (E-ring) in Mg-protoporphyrin monomethyl ester (MPE) to yield protochlorophyllide a (PChlide a) via a six-electron oxidation and the formation of an oxo group at position C13 using oxygen from a water molecule.1 Publication1 Publication

Catalytic activityi

Magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O = 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi204Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi208Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi211Iron-sulfur (4Fe-4S)1 Publication1

GO - Molecular functioni

GO - Biological processi

  • bacteriochlorophyll biosynthetic process Source: CACAO
  • light-independent bacteriochlorophyll biosynthetic process Source: UniProtKB-UniPathway
  • photosynthesis Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processBacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis
Ligand4Fe-4S, Cobalamin, Cobalt, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13265
UniPathwayiUPA00671

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase1 Publication (EC:1.21.98.31 Publication)
Short name:
MPE cyclase1 Publication
Gene namesi
Name:bchE1 Publication
Ordered Locus Names:RCAP_rcc00669
OrganismiRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Taxonomic identifieri272942 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002361 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000648721 – 575Anaerobic magnesium-protoporphyrin IX monomethyl ester cyclaseAdd BLAST575

Interactioni

Protein-protein interaction databases

STRINGi272942.RCAP_rcc00669

Structurei

3D structure databases

ProteinModelPortaliP26168
SMRiP26168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 143B12-bindingPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Belongs to the BchE family.Curated

Phylogenomic databases

eggNOGiENOG4105EPH Bacteria
COG1032 LUCA
HOGENOMiHOG000154354
KOiK04034
OMAiPDNMERE
OrthoDBiPOG091H0L7I

Family and domain databases

Gene3Di3.80.30.20, 1 hit
InterProiView protein in InterPro
IPR011772 BchE
IPR006158 Cobalamin-bd
IPR036724 Cobalamin-bd_sf
IPR006638 Elp3/MiaB/NifB
IPR034466 Methyltransferase_Class_B
IPR007197 rSAM
IPR023404 rSAM_horseshoe
PfamiView protein in Pfam
PF02310 B12-binding, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDF00302 anaerobic_magnesium-protoporph, 1 hit
SFLDG01123 methyltransferase_(Class_B), 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
SUPFAMiSSF52242 SSF52242, 1 hit
TIGRFAMsiTIGR02026 BchE, 1 hit
PROSITEiView protein in PROSITE
PS51332 B12_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P26168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILFVHPNY HSGGAEIAGN WPPSWVPYLA GHLKKAGFDD IHFIDAMTLN
60 70 80 90 100
VSHDELRKKF AELQPDLIGV TSITPSIYEA EETLKIAKEV VPNAVRVLGG
110 120 130 140 150
VHATFMFRQV LSEAPWVDAI VRGEGEEIMV ELAKCVSEGR WPEDRASIKG
160 170 180 190 200
LAFHDGTEIV ATQAAPTVKD IDSLKPDWSL IDWKHYIYIP LGVRVAIPNM
210 220 230 240 250
ARGCPFTCSF CSQWKFWRDY RVRSPKAVVD EIEDLVNNYD VGFFILADEE
260 270 280 290 300
PTINKKKFVE FCQEMIDRGL NHKVKWGINT RVTDIYRDRD LLKFYREAGL
310 320 330 340 350
VHISLGTEAA AQLKLDLFNK ETTVAENKEA IRLLREADIF TEAQFIVGLD
360 370 380 390 400
NETKETLEET FQMAWDWQPD LANWSMYTPW PFTPLFQELR DQVEVFDFSK
410 420 430 440 450
YNFVTPIMKP KALTRGELLD GVMKNYRRFY MRKALFHYPW RGTGFRRRYL
460 470 480 490 500
LGCLKAFLKA GVGRTFYDLG KAGYWGPQTK DTVDFHFDET RKIAEAQVAD
510 520 530 540 550
WEAAADRSRK HKERQEALRA QMKDRAADRN TANFVMPADA EDEFDLSAET
560 570
HEARSAEHAA MACGGGKDQM VDAAE
Length:575
Mass (Da):65,866
Last modified:May 1, 1992 - v1
Checksum:iA1814FBF9D877310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11165 Genomic DNA Translation: CAA77530.1
CP001312 Genomic DNA Translation: ADE84434.1
PIRiS17814
RefSeqiWP_013066413.1, NC_014034.1

Genome annotation databases

EnsemblBacteriaiADE84434; ADE84434; RCAP_rcc00669
GeneIDi31489615
KEGGircp:RCAP_rcc00669

Similar proteinsi

Entry informationi

Entry nameiBCHE_RHOCB
AccessioniPrimary (citable) accession number: P26168
Secondary accession number(s): D5ANT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 20, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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