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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Gene

Hsd3b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH.
A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Oxidoreductase
Biological processSteroidogenesis
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-193993 Mineralocorticoid biosynthesis
R-MMU-194002 Glucocorticoid biosynthesis
UniPathwayi
UPA00062

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
Short name:
3-beta-HSD II
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96234 Hsd3b2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877811 – 3733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2Add BLAST373

Proteomic databases

MaxQBiP26149
PaxDbiP26149
PeptideAtlasiP26149
PRIDEiP26149

PTM databases

iPTMnetiP26149
PhosphoSitePlusiP26149

Expressioni

Tissue specificityi

Liver and kidney.

Gene expression databases

BgeeiENSMUSG00000063730 Expressed in 33 organ(s), highest expression level in kidney
GenevisibleiP26149 MM

Interactioni

Protein-protein interaction databases

BioGridi200438, 1 interactor
STRINGi10090.ENSMUSP00000102635

Structurei

3D structure databases

ProteinModelPortaliP26149
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430 Eukaryota
COG0451 LUCA
GeneTreeiENSGT00550000074557
HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiP26149
KOiK00070
OMAiHIVTLSN
OrthoDBiEOG091G09QZ
TreeFamiTF343138

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

P26149-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGWSCLVTG AGGFLGQRII QLLVQEEDLE EIRVLDKVFR PETRKEFFNL
60 70 80 90 100
ETSIKVTVLE GDILDTQYLR RACQGISVVI HTAAIIDVTG VIPRQTILDV
110 120 130 140 150
NLKGTQNLLE ACIQASVPAF IFSSSVDVAG PNSYKEIVLN GHEEECHEST
160 170 180 190 200
WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL QTCALRPMCI YGERSPLISN
210 220 230 240 250
IIIMALKHKG ILRSFGKFNT ANPVYVGNVA WAHILAARGL RDPKKSPNIQ
260 270 280 290 300
GEFYYISDDT PHQSFDDISY TLSKEWGFCL DSSWSLPVPL LYWLAFLLET
310 320 330 340 350
VSFLLSPIYR YIPPFNRHLV TLSGSTFTFS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):41,923
Last modified:June 28, 2011 - v4
Checksum:i2500AF16F38C081B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12G → K in AAH26757 (PubMed:15489334).Curated1
Sequence conflicti12G → K in AAH40397 (PubMed:15489334).Curated1
Sequence conflicti54I → N in AAH26757 (PubMed:15489334).Curated1
Sequence conflicti54I → N in AAH40397 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606755 Genomic DNA No translation available.
BC026757 mRNA Translation: AAH26757.1
BC040397 mRNA Translation: AAH40397.1
M75886 mRNA No translation available.
CCDSiCCDS17667.1
RefSeqiNP_694873.2, NM_153193.3
XP_006501101.1, XM_006501038.3
UniGeneiMm.482364

Genome annotation databases

EnsembliENSMUST00000107021; ENSMUSP00000102635; ENSMUSG00000063730
ENSMUST00000107022; ENSMUSP00000102636; ENSMUSG00000063730
ENSMUST00000177651; ENSMUSP00000136533; ENSMUSG00000063730
GeneIDi15493
KEGGimmu:15493
UCSCiuc008qqc.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606755 Genomic DNA No translation available.
BC026757 mRNA Translation: AAH26757.1
BC040397 mRNA Translation: AAH40397.1
M75886 mRNA No translation available.
CCDSiCCDS17667.1
RefSeqiNP_694873.2, NM_153193.3
XP_006501101.1, XM_006501038.3
UniGeneiMm.482364

3D structure databases

ProteinModelPortaliP26149
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200438, 1 interactor
STRINGi10090.ENSMUSP00000102635

PTM databases

iPTMnetiP26149
PhosphoSitePlusiP26149

Proteomic databases

MaxQBiP26149
PaxDbiP26149
PeptideAtlasiP26149
PRIDEiP26149

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107021; ENSMUSP00000102635; ENSMUSG00000063730
ENSMUST00000107022; ENSMUSP00000102636; ENSMUSG00000063730
ENSMUST00000177651; ENSMUSP00000136533; ENSMUSG00000063730
GeneIDi15493
KEGGimmu:15493
UCSCiuc008qqc.2 mouse

Organism-specific databases

CTDi3284
MGIiMGI:96234 Hsd3b2

Phylogenomic databases

eggNOGiKOG1430 Eukaryota
COG0451 LUCA
GeneTreeiENSGT00550000074557
HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiP26149
KOiK00070
OMAiHIVTLSN
OrthoDBiEOG091G09QZ
TreeFamiTF343138

Enzyme and pathway databases

UniPathwayi
UPA00062

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-193993 Mineralocorticoid biosynthesis
R-MMU-194002 Glucocorticoid biosynthesis

Miscellaneous databases

PROiPR:P26149
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063730 Expressed in 33 organ(s), highest expression level in kidney
GenevisibleiP26149 MM

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3BHS2_MOUSE
AccessioniPrimary (citable) accession number: P26149
Secondary accession number(s): B1ARN7, Q8R0J6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 28, 2011
Last modified: November 7, 2018
This is version 155 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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