UniProtKB - P26049 (GBRA3_MOUSE)
Protein
Gamma-aminobutyric acid receptor subunit alpha-3
Gene
Gabra3
Organism
Mus musculus (Mouse)
Status
Functioni
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
GO - Molecular functioni
- chloride channel activity Source: GO_Central
- GABA-A receptor activity Source: MGI
- GABA-gated chloride ion channel activity Source: GO_Central
- inhibitory extracellular ligand-gated ion channel activity Source: GO_Central
- neurotransmitter receptor activity Source: GO_Central
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: SynGO
GO - Biological processi
- chemical synaptic transmission Source: MGI
- chloride transmembrane transport Source: GO_Central
- gamma-aminobutyric acid signaling pathway Source: GO_Central
- ion transmembrane transport Source: GO_Central
- nervous system process Source: GO_Central
- regulation of membrane potential Source: GO_Central
- regulation of postsynaptic membrane potential Source: GO_Central
- signal transduction Source: GO_Central
- synaptic transmission, GABAergic Source: GO_Central
Keywordsi
Molecular function | Chloride channel, Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Ligand | Chloride |
Enzyme and pathway databases
Reactomei | R-MMU-977443, GABA receptor activation |
Names & Taxonomyi
Protein namesi | Recommended name: Gamma-aminobutyric acid receptor subunit alpha-3Alternative name(s): GABA(A) receptor subunit alpha-3 |
Gene namesi | Name:Gabra3 Synonyms:Gabra-3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95615, Gabra3 |
Subcellular locationi
Plasma Membrane
- dendrite membrane Source: GO_Central
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic specialization membrane Source: SynGO
Other locations
- chloride channel complex Source: UniProtKB-KW
- GABA-A receptor complex Source: GO_Central
- GABA-ergic synapse Source: SynGO
- neuron projection Source: GO_Central
- postsynapse Source: GO_Central
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 29 – 276 | ExtracellularCuratedAdd BLAST | 248 | |
Transmembranei | 277 – 298 | HelicalCuratedAdd BLAST | 22 | |
Transmembranei | 304 – 325 | HelicalCuratedAdd BLAST | 22 | |
Transmembranei | 338 – 359 | HelicalCuratedAdd BLAST | 22 | |
Topological domaini | 360 – 457 | CytoplasmicCuratedAdd BLAST | 98 | |
Transmembranei | 458 – 479 | HelicalCuratedAdd BLAST | 22 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | Sequence analysisAdd BLAST | 28 | |
ChainiPRO_0000000438 | 29 – 492 | Gamma-aminobutyric acid receptor subunit alpha-3Add BLAST | 464 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 63 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 163 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 191 ↔ 205 | By similarity | ||
Glycosylationi | 228 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 426 | PhosphoserineCombined sources | 1 | |
Modified residuei | 427 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 433 | PhosphoserineCombined sources | 1 | |
Modified residuei | 442 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P26049 |
PaxDbi | P26049 |
PRIDEi | P26049 |
PTM databases
GlyConnecti | 2322, 8 N-Linked glycans (3 sites) |
GlyGeni | P26049, 4 sites |
iPTMneti | P26049 |
PhosphoSitePlusi | P26049 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031343, Expressed in frontal cortex and 175 other tissues |
ExpressionAtlasi | P26049, baseline and differential |
Genevisiblei | P26049, MM |
Interactioni
Subunit structurei
Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1 (PubMed:11528422).
Interacts with GPHN (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 199799, 1 interactor |
ComplexPortali | CPX-2982, GABA-A receptor, alpha-3/beta-3/gamma-2 |
STRINGi | 10090.ENSMUSP00000062638 |
Miscellaneous databases
RNActi | P26049, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA3 sub-subfamily. [View classification]Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3642, Eukaryota |
GeneTreei | ENSGT00940000159444 |
HOGENOMi | CLU_010920_2_2_1 |
InParanoidi | P26049 |
OMAi | NEVVYIW |
OrthoDBi | 1057372at2759 |
PhylomeDBi | P26049 |
TreeFami | TF315453 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006028, GABAA/Glycine_rcpt IPR001390, GABAAa_rcpt IPR005433, GABBAa3_rcpt IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01079, GABAARALPHA PR01616, GABAARALPHA3 PR00253, GABAARECEPTR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P26049-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIITQMWHFY VTRVVLLLLI SILPGTTSQG ESRRQEPGDF VKQDIGGLSP
60 70 80 90 100
KHAPDIPDDS TDNITIFTRI LDRLLDGYDN RLRPGLGDAV TEVKTDIYVT
110 120 130 140 150
SFGPVSDTDM EYTIDVFFRQ TWHDERLKFD GPMKILPLNN LLASKIWTPD
160 170 180 190 200
TFFHNGKKSV AHNMTTPNKL LRLVDNGTLL YTMRLTIHAE CPMHLEDFPM
210 220 230 240 250
DVHACPLKFG SYAYTKAEVI YSWTLGKNKS VEVAQDGSRL NQYDLLGHVV
260 270 280 290 300
GTEIIRSSTG EYVVMTTHFH LKRKIGYFVI QTYLPCIMTV ILSQVSFWLN
310 320 330 340 350
RESVPARTVF GVTTVLTMTT LSISARNSLP KVAYATAMDW FIAVCYAFVF
360 370 380 390 400
SALIEFATVN YFTKRSWAWE GKKVPEALEM KKKTPAAPTK KNTTFNIVGT
410 420 430 440 450
TYPINLAKDT EFSTISKSAA APSASSTPTA IASPKATYVQ DSPAETKTYN
460 470 480 490
SVSKVDKISR IIFPVLFAIF NLVYWATYVN RESAIKGMIR KQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB1AVY2 | B1AVY2_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra3 | 533 | Annotation score: |
RNA editingi
Edited at position 342.1 Publication
The extent of editing is low at birth but increases with age, reaching close to 100% in the adult brain.
The extent of editing is low at birth but increases with age, reaching close to 100% in the adult brain.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 342 | I → M in RNA edited version. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86568 mRNA Translation: AAA37651.1 |
CCDSi | CCDS30187.1 |
RefSeqi | NP_032093.3, NM_008067.4 XP_006527886.1, XM_006527823.3 XP_011245824.1, XM_011247522.2 |
Genome annotation databases
Ensembli | ENSMUST00000055966; ENSMUSP00000062638; ENSMUSG00000031343 |
GeneIDi | 14396 |
KEGGi | mmu:14396 |
UCSCi | uc009tkp.2, mouse |
Keywords - Coding sequence diversityi
RNA editingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86568 mRNA Translation: AAA37651.1 |
CCDSi | CCDS30187.1 |
RefSeqi | NP_032093.3, NM_008067.4 XP_006527886.1, XM_006527823.3 XP_011245824.1, XM_011247522.2 |
3D structure databases
SMRi | P26049 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199799, 1 interactor |
ComplexPortali | CPX-2982, GABA-A receptor, alpha-3/beta-3/gamma-2 |
STRINGi | 10090.ENSMUSP00000062638 |
Chemistry databases
ChEMBLi | CHEMBL2094133 |
DrugCentrali | P26049 |
PTM databases
GlyConnecti | 2322, 8 N-Linked glycans (3 sites) |
GlyGeni | P26049, 4 sites |
iPTMneti | P26049 |
PhosphoSitePlusi | P26049 |
Proteomic databases
MaxQBi | P26049 |
PaxDbi | P26049 |
PRIDEi | P26049 |
Protocols and materials databases
Antibodypediai | 335, 213 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000055966; ENSMUSP00000062638; ENSMUSG00000031343 |
GeneIDi | 14396 |
KEGGi | mmu:14396 |
UCSCi | uc009tkp.2, mouse |
Organism-specific databases
CTDi | 2556 |
MGIi | MGI:95615, Gabra3 |
Phylogenomic databases
eggNOGi | KOG3642, Eukaryota |
GeneTreei | ENSGT00940000159444 |
HOGENOMi | CLU_010920_2_2_1 |
InParanoidi | P26049 |
OMAi | NEVVYIW |
OrthoDBi | 1057372at2759 |
PhylomeDBi | P26049 |
TreeFami | TF315453 |
Enzyme and pathway databases
Reactomei | R-MMU-977443, GABA receptor activation |
Miscellaneous databases
BioGRID-ORCSi | 14396, 2 hits in 18 CRISPR screens |
ChiTaRSi | Gabra3, mouse |
PROi | PR:P26049 |
RNActi | P26049, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031343, Expressed in frontal cortex and 175 other tissues |
ExpressionAtlasi | P26049, baseline and differential |
Genevisiblei | P26049, MM |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006028, GABAA/Glycine_rcpt IPR001390, GABAAa_rcpt IPR005433, GABBAa3_rcpt IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01079, GABAARALPHA PR01616, GABAARALPHA3 PR00253, GABAARECEPTR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GBRA3_MOUSE | |
Accessioni | P26049Primary (citable) accession number: P26049 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families