UniProtKB - P25976 (UBF1_MOUSE)
Nucleolar transcription factor 1
Ubtf
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 112 – 180 | HMG box 1PROSITE-ProRule annotationAdd BLAST | 69 | |
DNA bindingi | 196 – 264 | HMG box 2PROSITE-ProRule annotationAdd BLAST | 69 | |
DNA bindingi | 298 – 362 | HMG box 3PROSITE-ProRule annotationAdd BLAST | 65 | |
DNA bindingi | 407 – 475 | HMG box 4PROSITE-ProRule annotationAdd BLAST | 69 | |
DNA bindingi | 482 – 549 | HMG box 5PROSITE-ProRule annotationAdd BLAST | 68 | |
DNA bindingi | 568 – 634 | HMG box 6PROSITE-ProRule annotationAdd BLAST | 67 |
GO - Molecular functioni
- chromatin binding Source: UniProtKB
- RNA polymerase I cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase I core promoter sequence-specific DNA binding Source: MGI
- RNA polymerase I general transcription initiation factor activity Source: MGI
- scaffold protein binding Source: MGI
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: MGI
- positive regulation of transcription by RNA polymerase I Source: UniProtKB
- rDNA heterochromatin assembly Source: MGI
- transcription by RNA polymerase I Source: MGI
- transcription elongation from RNA polymerase I promoter Source: MGI
- transcription initiation from RNA polymerase I promoter Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-73728, RNA Polymerase I Promoter Opening R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-73772, RNA Polymerase I Promoter Escape R-MMU-73863, RNA Polymerase I Transcription Termination |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleolar transcription factor 1Alternative name(s): Upstream-binding factor 1 Short name: UBF-1 |
Gene namesi | Name:Ubtf Synonyms:Tcfubf, Ubf-1, Ubf1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98512, Ubtf |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
Nucleus
- fibrillar center Source: MGI
- nucleolus Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048626 | 1 – 765 | Nucleolar transcription factor 1Add BLAST | 765 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 201 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 273 | Phosphoserine1 Publication | 1 | |
Modified residuei | 336 | Phosphoserine1 Publication | 1 | |
Modified residuei | 364 | Phosphoserine1 Publication | 1 | |
Modified residuei | 389 | Phosphoserine1 Publication | 1 | |
Modified residuei | 412 | Phosphoserine1 Publication | 1 | |
Modified residuei | 433 | Phosphoserine1 Publication | 1 | |
Modified residuei | 435 | PhosphoserineBy similarity | 1 | |
Modified residuei | 484 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 495 | PhosphoserineBy similarity | 1 | |
Modified residuei | 546 | Phosphoserine1 Publication | 1 | |
Modified residuei | 584 | Phosphoserine1 Publication | 1 | |
Modified residuei | 638 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P25976 |
jPOSTi | P25976 |
MaxQBi | P25976 |
PaxDbi | P25976 |
PeptideAtlasi | P25976 |
PRIDEi | P25976 |
ProteomicsDBi | 298449 [P25976-1] 298450 [P25976-2] |
PTM databases
iPTMneti | P25976 |
PhosphoSitePlusi | P25976 |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with TBP (By similarity).
Interacts with TAF1A (By similarity).
Interacts with RASL11A (PubMed:20168301). Binds to IRS1 and PIK3CA (PubMed:15197263).
Interacts with DHX33 (PubMed:21930779).
Interacts with PHF6 (By similarity).
Interacts with CEBPA (isoform 1 and isoform 4) (By similarity).
Interacts with DDX11 (By similarity).
Interacts with NOP53 (By similarity).
By similarity3 PublicationsBinary interactionsi
P25976
With | #Exp. | IntAct |
---|---|---|
Runx2 [Q08775] | 4 | EBI-7364139,EBI-903354 |
GO - Molecular functioni
- scaffold protein binding Source: MGI
Protein-protein interaction databases
DIPi | DIP-29977N |
ELMi | P25976 |
IntActi | P25976, 12 interactors |
MINTi | P25976 |
STRINGi | 10090.ENSMUSP00000133844 |
Miscellaneous databases
RNActi | P25976, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P25976 |
SMRi | P25976 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P25976 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 675 – 765 | Asp/Glu/Ser-rich (acidic)Add BLAST | 91 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0381, Eukaryota |
InParanoidi | P25976 |
PhylomeDBi | P25976 |
Family and domain databases
Gene3Di | 1.10.30.10, 6 hits |
InterProi | View protein in InterPro IPR029215, HMG_box_5 IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf |
Pfami | View protein in Pfam PF00505, HMG_box, 3 hits PF09011, HMG_box_2, 1 hit PF14887, HMG_box_5, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 6 hits |
SUPFAMi | SSF47095, SSF47095, 6 hits |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 6 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE
60 70 80 90 100
SHMDWEKVAF KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY
110 120 130 140 150
KGKKLKKHPD FPKKPLTPYF RFFMEKRAKY AKLHPEMSNL DLTKILSKKY
160 170 180 190 200
KELPEKKKMK YIQDFQREKQ EFERNLARFR EDHPDLIQNA KKSDIPEKPK
210 220 230 240 250
TPQQLWYTHE KKVYLKVRPD ATTKEVKDSL GKQWSQLSDK KTLKWIHKAL
260 270 280 290 300
EQRKEYEEIM RDYIQKHPEL NISEEGITKS TLTKAERQLK DKFDGRPTKP
310 320 330 340 350
PPNSYSLYCA ELMANMKDVP STERMVLCSQ QWKLLSQKEK DAYHKKCDQK
360 370 380 390 400
KKDYEVELLR FLESLPEEEQ QRVLGEEKML NINKKQTTSP ASKKPSQEGG
410 420 430 440 450
KGGSEKPKRP VSAMFIFSEE KRRQLQEERP ELSESELTRL LARMWNDLTE
460 470 480 490 500
KKKAKYKARE AALKAQSERK PGGEREDRGK LPESPKRAEE IWQQSVIGDY
510 520 530 540 550
LARFKNDRVK ALKAMEMTWN NMEKKEKLMW IKKAAEDQKR YERELSEMRA
560 570 580 590 600
PPAATNSSKK MKFQGEPKKP PMNGYQKFSQ ELLSNGELNH LPLKERMVEI
610 620 630 640 650
GSRWQRISQS QKEHYKKLAE EQQRQYKVHL DLWVKSLSPQ DRAAYKEYIS
660 670 680 690 700
NKRKNMTKLR GPNPKSSRTT LQSKSESEED DDEEEEDDEE EEEEEEDDEN
710 720 730 740 750
GDSSEDGGDS SESSSEDESE DGDENDDDDD DEDDEDDDDE DEDNESEGSS
760
SSSSSSGDSS DSGSN
The sequence of this isoform differs from the canonical sequence as follows:
221-257: Missing.
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AWT5 | A2AWT5_MOUSE | Nucleolar transcription factor 1 | Ubtf | 764 | Annotation score: | ||
A2AWT6 | A2AWT6_MOUSE | Nucleolar transcription factor 1 | Ubtf | 727 | Annotation score: | ||
E9Q4A8 | E9Q4A8_MOUSE | Nucleolar transcription factor 1 | Ubtf | 169 | Annotation score: | ||
G3UZW9 | G3UZW9_MOUSE | Nucleolar transcription factor 1 | Ubtf | 151 | Annotation score: | ||
A2AWT7 | A2AWT7_MOUSE | Nucleolar transcription factor 1 | Ubtf | 752 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002194 | 221 – 257 | Missing in isoform UBF2. 1 PublicationAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60831 mRNA Translation: CAA43222.1 |
PIRi | S22314 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60831 mRNA Translation: CAA43222.1 |
PIRi | S22314 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1V63 | NMR | - | A | 567-654 | [»] | |
1V64 | NMR | - | A | 288-382 | [»] | |
1WGF | NMR | - | A | 394-470 | [»] | |
BMRBi | P25976 | |||||
SMRi | P25976 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-29977N |
ELMi | P25976 |
IntActi | P25976, 12 interactors |
MINTi | P25976 |
STRINGi | 10090.ENSMUSP00000133844 |
PTM databases
iPTMneti | P25976 |
PhosphoSitePlusi | P25976 |
Proteomic databases
EPDi | P25976 |
jPOSTi | P25976 |
MaxQBi | P25976 |
PaxDbi | P25976 |
PeptideAtlasi | P25976 |
PRIDEi | P25976 |
ProteomicsDBi | 298449 [P25976-1] 298450 [P25976-2] |
Organism-specific databases
MGIi | MGI:98512, Ubtf |
Phylogenomic databases
eggNOGi | KOG0381, Eukaryota |
InParanoidi | P25976 |
PhylomeDBi | P25976 |
Enzyme and pathway databases
Reactomei | R-MMU-73728, RNA Polymerase I Promoter Opening R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-73772, RNA Polymerase I Promoter Escape R-MMU-73863, RNA Polymerase I Transcription Termination |
Miscellaneous databases
ChiTaRSi | Ubtf, mouse |
EvolutionaryTracei | P25976 |
PROi | PR:P25976 |
RNActi | P25976, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.10.30.10, 6 hits |
InterProi | View protein in InterPro IPR029215, HMG_box_5 IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf |
Pfami | View protein in Pfam PF00505, HMG_box, 3 hits PF09011, HMG_box_2, 1 hit PF14887, HMG_box_5, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 6 hits |
SUPFAMi | SSF47095, SSF47095, 6 hits |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBF1_MOUSE | |
Accessioni | P25976Primary (citable) accession number: P25976 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references