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UniProtKB - P25906 (PDXI_ECOLI)
Protein
Pyridoxine 4-dehydrogenase
Gene
pdxI
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the NAD(P)H-dependent reduction of pyridoxal to pyridoxine in vitro. Is not able to reduce 4-pyridoxate, and to oxidize pyridoxine or pyridoxamine (PubMed:27941785).
Has Kemp eliminase activity towards the non-physiological substrate 5-nitrobenzisoxazole, producing 4-nitro-2-cyanophenol; this activity is not considered to be physiologically relevant (PubMed:21332126).
2 PublicationsCatalytic activityi
- EC:1.1.1.651 Publication
Kineticsi
kcat is 0.11 sec(-1) for the Kemp eliminase activity towards 5-nitrobenzisoxazole.1 Publication
- KM=270 µM for 5-nitrobenzisoxazole1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 59 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 210 – 218 | NADPBy similarity | 9 |
GO - Molecular functioni
- aldo-keto reductase (NADP) activity Source: GO_Central
- pyridoxine:NADP 4-dehydrogenase activity Source: EcoCyc
GO - Biological processi
- pyridoxal 5'-phosphate salvage Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11309-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdxI1 Publication Synonyms:ydbCImported Ordered Locus Names:b1406, JW1403 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070393 | 1 – 286 | Pyridoxine 4-dehydrogenaseAdd BLAST | 286 |
Proteomic databases
jPOSTi | P25906 |
PaxDbi | P25906 |
PRIDEi | P25906 |
2D gel databases
SWISS-2DPAGEi | P25906 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263019, 15 interactors |
DIPi | DIP-11632N |
IntActi | P25906, 4 interactors |
STRINGi | 511145.b1406 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0667, Bacteria |
HOGENOMi | CLU_023205_2_1_6 |
InParanoidi | P25906 |
OMAi | GYGTMQL |
PhylomeDBi | P25906 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
i Sequence
Sequence statusi: Complete.
P25906-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSNTFTLGT KSVNRLGYGA MQLAGPGVFG PPRDRHVAIT VLREALALGV
60 70 80 90 100
NHIDTSDFYG PHVTNQIIRE ALYPYSDDLT IVTKIGARRG EDASWLPAFS
110 120 130 140 150
PAELQKAVHD NLRNLGLDVL DVVNLRVMMG DGHGPAEGSI EASLTVLAEM
160 170 180 190 200
QQQGLVKHIG LSNVTPTQVA EARKIAEIVC VQNEYNIAHR ADDAMIDALA
210 220 230 240 250
HDGIAYVPFF PLGGFTPLQS STLSDVAASL GATPMQVALA WLLQRSPNIL
260 270 280
LIPGTSSVAH LRENMAAEKL HLSEEVLSTL DGISRE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62680 Genomic DNA Translation: CAA44553.1 U00096 Genomic DNA Translation: AAC74488.1 AP009048 Genomic DNA Translation: BAA15021.1 X97452 Genomic DNA Translation: CAA66103.1 |
PIRi | A48399 |
RefSeqi | NP_415924.1, NC_000913.3 WP_000097801.1, NZ_SSZK01000021.1 |
Genome annotation databases
EnsemblBacteriai | AAC74488; AAC74488; b1406 BAA15021; BAA15021; BAA15021 |
GeneIDi | 66674742 945980 |
KEGGi | ecj:JW1403 eco:b1406 |
PATRICi | fig|1411691.4.peg.865 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62680 Genomic DNA Translation: CAA44553.1 U00096 Genomic DNA Translation: AAC74488.1 AP009048 Genomic DNA Translation: BAA15021.1 X97452 Genomic DNA Translation: CAA66103.1 |
PIRi | A48399 |
RefSeqi | NP_415924.1, NC_000913.3 WP_000097801.1, NZ_SSZK01000021.1 |
3D structure databases
AlphaFoldDBi | P25906 |
SMRi | P25906 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263019, 15 interactors |
DIPi | DIP-11632N |
IntActi | P25906, 4 interactors |
STRINGi | 511145.b1406 |
2D gel databases
SWISS-2DPAGEi | P25906 |
Proteomic databases
jPOSTi | P25906 |
PaxDbi | P25906 |
PRIDEi | P25906 |
Protocols and materials databases
DNASUi | 945980 |
Genome annotation databases
EnsemblBacteriai | AAC74488; AAC74488; b1406 BAA15021; BAA15021; BAA15021 |
GeneIDi | 66674742 945980 |
KEGGi | ecj:JW1403 eco:b1406 |
PATRICi | fig|1411691.4.peg.865 |
Organism-specific databases
EchoBASEi | EB1285 |
Phylogenomic databases
eggNOGi | COG0667, Bacteria |
HOGENOMi | CLU_023205_2_1_6 |
InParanoidi | P25906 |
OMAi | GYGTMQL |
PhylomeDBi | P25906 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11309-MONOMER |
Miscellaneous databases
PROi | PR:P25906 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PDXI_ECOLI | |
Accessioni | P25906Primary (citable) accession number: P25906 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families