UniProtKB - P25894 (LOIP_ECOLI)
Protein
Metalloprotease LoiP
Gene
loiP
Organism
Escherichia coli (strain K12)
Status
Functioni
Metalloprotease that cleaves substrates preferentially between Phe-Phe residues. Plays a role in response to some stress conditions. Seems to regulate the expression of speB.4 Publications
Cofactori
Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 130 | Zinc; catalyticSequence analysis | 1 | |
Active sitei | 131 | Sequence analysis | 1 | |
Metal bindingi | 134 | Zinc; catalyticSequence analysis | 1 | |
Metal bindingi | 189 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: InterPro
- metallopeptidase activity Source: EcoCyc
GO - Biological processi
- cellular hypotonic response Source: EcoCyc
- cellular response to heat Source: EcoCyc
- cellular response to UV Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Stress response |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11291-MONOMER MetaCyc:EG11291-MONOMER |
Protein family/group databases
MEROPSi | M48.A04 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:loiP Synonyms:yggG Ordered Locus Names:b2936, JW2903 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane 2 Publications; Lipid-anchor PROSITE-ProRule annotation2 Publications Note: Proper membrane localization can depend on BepA.
GO - Cellular componenti
- cell outer membrane Source: EcoCyc
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | PROSITE-ProRule annotationAdd BLAST | 18 | |
ChainiPRO_0000138938 | 19 – 252 | Metalloprotease LoiPAdd BLAST | 234 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 19 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 19 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 53 ↔ 108 | 1 Publication |
Post-translational modificationi
The intramolecular disulfide bond improves the stability and the activity of LoiP. It forms even in the absence of the oxido-reductase DsbA (PubMed:22491786).1 Publication
Keywords - PTMi
Disulfide bond, Lipoprotein, PalmitateProteomic databases
jPOSTi | P25894 |
PaxDbi | P25894 |
PRIDEi | P25894 |
Expressioni
Inductioni
Induced by heat shock and low osmolarity.3 Publications
Interactioni
Subunit structurei
Interacts with Era and BepA.
2 PublicationsBinary interactionsi
P25894
With | #Exp. | IntAct |
---|---|---|
ydiB [P0A6D5] | 2 | EBI-560654,EBI-560638 |
Protein-protein interaction databases
BioGRIDi | 4263066, 14 interactors |
DIPi | DIP-12186N |
IntActi | P25894, 3 interactors |
STRINGi | 511145.b2936 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M48B family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0501, Bacteria |
HOGENOMi | CLU_074068_0_0_6 |
InParanoidi | P25894 |
PhylomeDBi | P25894 |
Family and domain databases
InterProi | View protein in InterPro IPR001915, Peptidase_M48 |
Pfami | View protein in Pfam PF01435, Peptidase_M48, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P25894-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIRALLVAM SVATVLTGCQ NMDSNGLLSS GAEAFQAYSL SDAQVKTLSD
60 70 80 90 100
QACQEMDSKA TIAPANSEYA KRLTTIANAL GNNINGQPVN YKVYMAKDVN
110 120 130 140 150
AFAMANGCIR VYSGLMDMMT DNEVEAVIGH EMGHVALGHV KKGMQVALGT
160 170 180 190 200
NAVRVAAASA GGIVGSLSQS QLGNLGEKLV NSQFSQRQEA EADDYSYDLL
210 220 230 240 250
RQRGISPAGL ATSFEKLAKL EEGRQSSMFD DHPASAERAQ HIRDRMSADG
IK
Sequence cautioni
The sequence AAA69103 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAA83911 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32363 Genomic DNA Translation: AAA83911.1 Different initiation. U28377 Genomic DNA Translation: AAA69103.1 Different initiation. U00096 Genomic DNA Translation: AAC75973.2 AP009048 Genomic DNA Translation: BAE76999.1 |
PIRi | G65078 |
RefSeqi | NP_417411.2, NC_000913.3 WP_001326497.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75973; AAC75973; b2936 BAE76999; BAE76999; BAE76999 |
GeneIDi | 945173 |
KEGGi | ecj:JW2903 eco:b2936 |
PATRICi | fig|1411691.4.peg.3797 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32363 Genomic DNA Translation: AAA83911.1 Different initiation. U28377 Genomic DNA Translation: AAA69103.1 Different initiation. U00096 Genomic DNA Translation: AAC75973.2 AP009048 Genomic DNA Translation: BAE76999.1 |
PIRi | G65078 |
RefSeqi | NP_417411.2, NC_000913.3 WP_001326497.1, NZ_LN832404.1 |
3D structure databases
SMRi | P25894 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263066, 14 interactors |
DIPi | DIP-12186N |
IntActi | P25894, 3 interactors |
STRINGi | 511145.b2936 |
Protein family/group databases
MEROPSi | M48.A04 |
Proteomic databases
jPOSTi | P25894 |
PaxDbi | P25894 |
PRIDEi | P25894 |
Genome annotation databases
EnsemblBacteriai | AAC75973; AAC75973; b2936 BAE76999; BAE76999; BAE76999 |
GeneIDi | 945173 |
KEGGi | ecj:JW2903 eco:b2936 |
PATRICi | fig|1411691.4.peg.3797 |
Organism-specific databases
EchoBASEi | EB1268 |
Phylogenomic databases
eggNOGi | COG0501, Bacteria |
HOGENOMi | CLU_074068_0_0_6 |
InParanoidi | P25894 |
PhylomeDBi | P25894 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11291-MONOMER MetaCyc:EG11291-MONOMER |
Miscellaneous databases
PROi | PR:P25894 |
Family and domain databases
InterProi | View protein in InterPro IPR001915, Peptidase_M48 |
Pfami | View protein in Pfam PF01435, Peptidase_M48, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LOIP_ECOLI | |
Accessioni | P25894Primary (citable) accession number: P25894 Secondary accession number(s): P76646, Q2M9Q7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries