Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent RNA helicase RhlE

Gene

rhlE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DEAD-box RNA helicase involved in ribosome assembly. Has RNA-dependent ATPase activity and unwinds double-stranded RNA. May play a role in the interconversion of ribosomal RNA-folding intermediates that are further processed by DeaD or SrmB during ribosome maturation.UniRule annotation2 Publications

Miscellaneous

In vitro, can replace RhlB in degradosome and facilitate the degradation of RNA by PNPase.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation1 Publication

Enzyme regulationi

ATPase activity is stimulated by both long RNAs and short oligoribonucleotides.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • response to heat Source: EcoCyc
  • ribosome assembly Source: EcoCyc
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11235-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase RhlEUniRule annotation (EC:3.6.4.13UniRule annotation)
Gene namesi
Name:rhlEUniRule annotation
Ordered Locus Names:b0797, JW0781
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11235 rhlE

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication
  • Note: Ribosome-associated. Can either bind to the intact 70S ribosome or to individual ribosomal subunits.

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551081 – 454ATP-dependent RNA helicase RhlEAdd BLAST454

Proteomic databases

PaxDbiP25888
PRIDEiP25888

Expressioni

Inductioni

Transiently induced by cold shock in a PNPase-dependent fashion (PubMed:14527658). Induced by the transcriptional regulator CecR (PubMed:27112147).2 Publications

Interactioni

Subunit structurei

Interacts with PcnB. Interacts in vitro with RNase E.2 Publications

Protein-protein interaction databases

BioGridi4259962, 113 interactors
DIPiDIP-10697N
IntActiP25888, 35 interactors
STRINGi316385.ECDH10B_0865

Structurei

3D structure databases

ProteinModelPortaliP25888
SMRiP25888
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 208Helicase ATP-bindingUniRule annotationAdd BLAST177
Domaini219 – 381Helicase C-terminalUniRule annotationAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 29Q motifAdd BLAST29
Motifi156 – 159DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family. RhlE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1J Bacteria
COG0513 LUCA
HOGENOMiHOG000268807
InParanoidiP25888
KOiK11927
OMAiLNMDFEV
PhylomeDBiP25888

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00968 DEAD_helicase_RhlE, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR028622 DEAD_helicase_RhlE
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P25888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFDSLGLSP DILRAVAEQG YREPTPIQQQ AIPAVLEGRD LMASAQTGTG
60 70 80 90 100
KTAGFTLPLL QHLITRQPHA KGRRPVRALI LTPTRELAAQ IGENVRDYSK
110 120 130 140 150
YLNIRSLVVF GGVSINPQMM KLRGGVDVLV ATPGRLLDLE HQNAVKLDQV
160 170 180 190 200
EILVLDEADR MLDMGFIHDI RRVLTKLPAK RQNLLFSATF SDDIKALAEK
210 220 230 240 250
LLHNPLEIEV ARRNTASDQV TQHVHFVDKK RKRELLSHMI GKGNWQQVLV
260 270 280 290 300
FTRTKHGANH LAEQLNKDGI RSAAIHGNKS QGARTRALAD FKSGDIRVLV
310 320 330 340 350
ATDIAARGLD IEELPHVVNY ELPNVPEDYV HRIGRTGRAA ATGEALSLVC
360 370 380 390 400
VDEHKLLRDI EKLLKKEIPR IAIPGYEPDP SIKAEPIQNG RQQRGGGGRG
410 420 430 440 450
QGGGRGQQQP RRGEGGAKSA SAKPAEKPSR RLGDAKPAGE QQRRRRPRKP

AAAQ
Length:454
Mass (Da):49,989
Last modified:June 1, 1994 - v3
Checksum:i7576B35B7901671F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55F → G in X56307 (PubMed:1931833).Curated1
Sequence conflicti107L → R in X56307 (PubMed:1931833).Curated1
Sequence conflicti210V → A in X56307 (PubMed:1931833).Curated1
Sequence conflicti230K → E in X56307 (PubMed:1931833).Curated1
Sequence conflicti239M → L in X56307 (PubMed:1931833).Curated1
Sequence conflicti258A → G in X56307 (PubMed:1931833).Curated1
Sequence conflicti286 – 287RA → LS in X56307 (PubMed:1931833).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02123 Genomic DNA Translation: AAA53653.1
U00096 Genomic DNA Translation: AAC73884.1
AP009048 Genomic DNA Translation: BAA35457.1
X56307 Genomic DNA No translation available.
PIRiE64816
RefSeqiNP_415318.1, NC_000913.3
WP_000007101.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73884; AAC73884; b0797
BAA35457; BAA35457; BAA35457
GeneIDi945425
KEGGiecj:JW0781
eco:b0797
PATRICifig|1411691.4.peg.1481

Similar proteinsi

Entry informationi

Entry nameiRHLE_ECOLI
AccessioniPrimary (citable) accession number: P25888
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health