UniProtKB - P25888 (RHLE_ECOLI)
Protein
ATP-dependent RNA helicase RhlE
Gene
rhlE
Organism
Escherichia coli (strain K12)
Status
Functioni
DEAD-box RNA helicase involved in ribosome assembly. Has RNA-dependent ATPase activity and unwinds double-stranded RNA. May play a role in the interconversion of ribosomal RNA-folding intermediates that are further processed by DeaD or SrmB during ribosome maturation.UniRule annotation2 Publications
Miscellaneous
In vitro, can replace RhlB in degradosome and facilitate the degradation of RNA by PNPase.1 Publication
Catalytic activityi
- EC:3.6.4.13UniRule annotation1 Publication
Activity regulationi
ATPase activity is stimulated by both long RNAs and short oligoribonucleotides.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 52 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- RNA binding Source: UniProtKB-KW
- RNA helicase activity Source: EcoCyc
GO - Biological processi
- response to heat Source: EcoCyc
- ribosome assembly Source: EcoCyc
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Ribosome biogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11235-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rhlEUniRule annotation Ordered Locus Names:b0797, JW0781 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation1 Publication Note: Ribosome-associated. Can either bind to the intact 70S ribosome or to individual ribosomal subunits.
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055108 | 1 – 454 | ATP-dependent RNA helicase RhlEAdd BLAST | 454 |
Proteomic databases
jPOSTi | P25888 |
PaxDbi | P25888 |
PRIDEi | P25888 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Interacts with PcnB.
Interacts in vitro with RNase E.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4259962, 113 interactors 849799, 1 interactor |
DIPi | DIP-10697N |
IntActi | P25888, 35 interactors |
STRINGi | 511145.b0797 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 208 | Helicase ATP-bindingUniRule annotationAdd BLAST | 177 | |
Domaini | 219 – 381 | Helicase C-terminalUniRule annotationAdd BLAST | 163 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1 – 29 | Q motifAdd BLAST | 29 | |
Motifi | 156 – 159 | DEAD box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0513, Bacteria |
HOGENOMi | CLU_003041_28_3_6 |
InParanoidi | P25888 |
PhylomeDBi | P25888 |
Family and domain databases
HAMAPi | MF_00968, DEAD_helicase_RhlE, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR028622, DEAD_helicase_RhlE IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
P25888-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFDSLGLSP DILRAVAEQG YREPTPIQQQ AIPAVLEGRD LMASAQTGTG
60 70 80 90 100
KTAGFTLPLL QHLITRQPHA KGRRPVRALI LTPTRELAAQ IGENVRDYSK
110 120 130 140 150
YLNIRSLVVF GGVSINPQMM KLRGGVDVLV ATPGRLLDLE HQNAVKLDQV
160 170 180 190 200
EILVLDEADR MLDMGFIHDI RRVLTKLPAK RQNLLFSATF SDDIKALAEK
210 220 230 240 250
LLHNPLEIEV ARRNTASDQV TQHVHFVDKK RKRELLSHMI GKGNWQQVLV
260 270 280 290 300
FTRTKHGANH LAEQLNKDGI RSAAIHGNKS QGARTRALAD FKSGDIRVLV
310 320 330 340 350
ATDIAARGLD IEELPHVVNY ELPNVPEDYV HRIGRTGRAA ATGEALSLVC
360 370 380 390 400
VDEHKLLRDI EKLLKKEIPR IAIPGYEPDP SIKAEPIQNG RQQRGGGGRG
410 420 430 440 450
QGGGRGQQQP RRGEGGAKSA SAKPAEKPSR RLGDAKPAGE QQRRRRPRKP
AAAQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 55 | F → G in X56307 (PubMed:1931833).Curated | 1 | |
Sequence conflicti | 107 | L → R in X56307 (PubMed:1931833).Curated | 1 | |
Sequence conflicti | 210 | V → A in X56307 (PubMed:1931833).Curated | 1 | |
Sequence conflicti | 230 | K → E in X56307 (PubMed:1931833).Curated | 1 | |
Sequence conflicti | 239 | M → L in X56307 (PubMed:1931833).Curated | 1 | |
Sequence conflicti | 258 | A → G in X56307 (PubMed:1931833).Curated | 1 | |
Sequence conflicti | 286 – 287 | RA → LS in X56307 (PubMed:1931833).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02123 Genomic DNA Translation: AAA53653.1 U00096 Genomic DNA Translation: AAC73884.1 AP009048 Genomic DNA Translation: BAA35457.1 X56307 Genomic DNA No translation available. |
PIRi | E64816 |
RefSeqi | NP_415318.1, NC_000913.3 WP_000007101.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73884; AAC73884; b0797 BAA35457; BAA35457; BAA35457 |
GeneIDi | 945425 |
KEGGi | ecj:JW0781 eco:b0797 |
PATRICi | fig|1411691.4.peg.1481 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02123 Genomic DNA Translation: AAA53653.1 U00096 Genomic DNA Translation: AAC73884.1 AP009048 Genomic DNA Translation: BAA35457.1 X56307 Genomic DNA No translation available. |
PIRi | E64816 |
RefSeqi | NP_415318.1, NC_000913.3 WP_000007101.1, NZ_SSZK01000002.1 |
3D structure databases
SMRi | P25888 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259962, 113 interactors 849799, 1 interactor |
DIPi | DIP-10697N |
IntActi | P25888, 35 interactors |
STRINGi | 511145.b0797 |
Proteomic databases
jPOSTi | P25888 |
PaxDbi | P25888 |
PRIDEi | P25888 |
Genome annotation databases
EnsemblBacteriai | AAC73884; AAC73884; b0797 BAA35457; BAA35457; BAA35457 |
GeneIDi | 945425 |
KEGGi | ecj:JW0781 eco:b0797 |
PATRICi | fig|1411691.4.peg.1481 |
Organism-specific databases
EchoBASEi | EB1217 |
Phylogenomic databases
eggNOGi | COG0513, Bacteria |
HOGENOMi | CLU_003041_28_3_6 |
InParanoidi | P25888 |
PhylomeDBi | P25888 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11235-MONOMER |
Miscellaneous databases
PROi | PR:P25888 |
Family and domain databases
HAMAPi | MF_00968, DEAD_helicase_RhlE, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR028622, DEAD_helicase_RhlE IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHLE_ECOLI | |
Accessioni | P25888Primary (citable) accession number: P25888 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families