UniProtKB - P25865 (UBC1_ARATH)
Protein
Ubiquitin-conjugating enzyme E2 1
Gene
UBC1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.2 Publications
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 88 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: GO_Central
- ubiquitin-protein transferase activity Source: TAIR
GO - Biological processi
- DNA repair Source: GO_Central
- histone H2B ubiquitination Source: TAIR
- histone ubiquitination Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein polyubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: TAIR
- vegetative to reproductive phase transition of meristem Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 1 (EC:2.3.2.23)Alternative name(s): E2 ubiquitin-conjugating enzyme 1 Ubiquitin carrier protein 1 Ubiquitin-conjugating enzyme E2-17 kDa 1 Ubiquitin-protein ligase 1 |
Gene namesi | Name:UBC1 Ordered Locus Names:At1g14400 ORF Names:F14L17.17, F14L17_35 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G14400 |
TAIRi | locus:2012622, AT1G14400 |
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 88 | C → S: Stabilization of the ester bond with the ubiquitin. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082570 | 1 – 152 | Ubiquitin-conjugating enzyme E2 1Add BLAST | 152 |
Proteomic databases
PaxDbi | P25865 |
PRIDEi | P25865 |
ProteomicsDBi | 228689 |
Expressioni
Tissue specificityi
Ubiquitously expressed.2 Publications
Inductioni
Not induced by heat shock.1 Publication
Gene expression databases
ExpressionAtlasi | P25865, baseline and differential |
Genevisiblei | P25865, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 23242, 3 interactors |
IntActi | P25865, 2 interactors |
STRINGi | 3702.AT1G14400.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P25865 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P25865 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 150 | UBC corePROSITE-ProRule annotationAdd BLAST | 147 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0419, Eukaryota |
HOGENOMi | CLU_030988_10_2_1 |
InParanoidi | P25865 |
OMAi | EDTIWEC |
OrthoDBi | 1292821at2759 |
PhylomeDBi | P25865 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P25865-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTPARKRLM RDFKRLQQDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG
60 70 80 90 100
GTFKLSLQFS EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY
110 120 130 140 150
DVAAILTSIQ SLLCDPNPNS PANSEAARMY SESKREYNRR VRDVVEQSWT
AD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M62721 mRNA Translation: AAA32903.1 L19351 Genomic DNA Translation: AAA32897.1 DQ027016 mRNA Translation: AAY44842.1 AC012188 Genomic DNA Translation: AAF43940.1 CP002684 Genomic DNA Translation: AEE29158.1 CP002684 Genomic DNA Translation: AEE29159.1 AF332451 mRNA Translation: AAG48814.1 AY070074 mRNA Translation: AAL49769.1 AY091330 mRNA Translation: AAM14269.1 AY085783 mRNA Translation: AAM63000.1 AK226391 mRNA Translation: BAE98537.1 Z27262 mRNA Translation: CAA81773.1 |
PIRi | S43781 |
RefSeqi | NP_563951.1, NM_101307.5 NP_973825.1, NM_202096.2 |
Genome annotation databases
EnsemblPlantsi | AT1G14400.1; AT1G14400.1; AT1G14400 AT1G14400.2; AT1G14400.2; AT1G14400 |
GeneIDi | 838002 |
Gramenei | AT1G14400.1; AT1G14400.1; AT1G14400 AT1G14400.2; AT1G14400.2; AT1G14400 |
KEGGi | ath:AT1G14400 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M62721 mRNA Translation: AAA32903.1 L19351 Genomic DNA Translation: AAA32897.1 DQ027016 mRNA Translation: AAY44842.1 AC012188 Genomic DNA Translation: AAF43940.1 CP002684 Genomic DNA Translation: AEE29158.1 CP002684 Genomic DNA Translation: AEE29159.1 AF332451 mRNA Translation: AAG48814.1 AY070074 mRNA Translation: AAL49769.1 AY091330 mRNA Translation: AAM14269.1 AY085783 mRNA Translation: AAM63000.1 AK226391 mRNA Translation: BAE98537.1 Z27262 mRNA Translation: CAA81773.1 |
PIRi | S43781 |
RefSeqi | NP_563951.1, NM_101307.5 NP_973825.1, NM_202096.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AAK | X-ray | 2.40 | A | 1-152 | [»] | |
SMRi | P25865 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 23242, 3 interactors |
IntActi | P25865, 2 interactors |
STRINGi | 3702.AT1G14400.1 |
Proteomic databases
PaxDbi | P25865 |
PRIDEi | P25865 |
ProteomicsDBi | 228689 |
Genome annotation databases
EnsemblPlantsi | AT1G14400.1; AT1G14400.1; AT1G14400 AT1G14400.2; AT1G14400.2; AT1G14400 |
GeneIDi | 838002 |
Gramenei | AT1G14400.1; AT1G14400.1; AT1G14400 AT1G14400.2; AT1G14400.2; AT1G14400 |
KEGGi | ath:AT1G14400 |
Organism-specific databases
Araporti | AT1G14400 |
TAIRi | locus:2012622, AT1G14400 |
Phylogenomic databases
eggNOGi | KOG0419, Eukaryota |
HOGENOMi | CLU_030988_10_2_1 |
InParanoidi | P25865 |
OMAi | EDTIWEC |
OrthoDBi | 1292821at2759 |
PhylomeDBi | P25865 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
EvolutionaryTracei | P25865 |
PROi | PR:P25865 |
Gene expression databases
ExpressionAtlasi | P25865, baseline and differential |
Genevisiblei | P25865, AT |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBC1_ARATH | |
Accessioni | P25865Primary (citable) accession number: P25865 Secondary accession number(s): Q4TZ08 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families