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Protein

Cyclin-dependent kinase B1-1

Gene

CDKB1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May control G2/M (mitosis) phase progression. Plays a role in regulating seedling growth in darkness via regulation of hypocotyl cell elongation and cotyledon cell development. Plays a role in stomatal development. Required to suppress endoreduplication. Together with CDKB1-2, promotes both the last division in the stomatal cell lineage as well as the number of stomata (PubMed:20675570). In collaboration with MYB124 and MYB88, restrict the G1/S transition and chloroplast and nuclear number during stomatal formation, and normally maintain fate and developmental progression throughout the stomatal cell lineage (PubMed:24123248, PubMed:24687979).8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Activity regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-176 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei142Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22 399

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B1-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB1;1
Alternative name(s):
Cell division control protein 2 homolog B
Gene namesi
Name:CDKB1-1
Synonyms:CDC2B
Ordered Locus Names:At3g54180
ORF Names:F24B22.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54180
TAIRilocus:2080290 AT3G54180

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No apparent stomatal abnormalities. The double mutant cdkb1;1 cdkb1;2 has a reduced number of abnormal stomata consisting in single guard cells (GC) (PubMed:20675570, PubMed:24123248, PubMed:24687979). CDKA-1 partially rescue abnormal stomata phenotype of cdkb1;1 cdkb1;2 (PubMed:24687979). The quadruple mutant flp-1 myb88 cdkb1;1 cdkb1;2 has a reduced number of large single guard cells blocked at mitosis, with strongly altered shape and size and characterized by enlarged nucleus due to endomitosis and endocycling, as well as extensive chloroplast replication (PubMed:24123248).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14T → A: Decreased kinase activity and disturbed cell cycle; when associated with F-15. 1 Publication1
Mutagenesisi15Y → F: Decreased kinase activity and disturbed cell cycle; when associated with A-14. 1 Publication1
Mutagenesisi161D → N: Dominant negative. Decreased kinase activity and disturbed cell cycle. 3 Publications1
Mutagenesisi171P → L: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi181T → I: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi249 – 251Missing : Decreased kinase activity and disturbed cell cycle. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857501 – 309Cyclin-dependent kinase B1-1Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphotyrosineBy similarity1
Modified residuei176Phosphothreonine; by CAKBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP25859
PRIDEiP25859

PTM databases

iPTMnetiP25859

Expressioni

Tissue specificityi

Highly expressed in guard cells and stomatal precursor cells of cotyledons. Expressed in roots, stems, flowers and siliques.2 Publications

Developmental stagei

Preferentially expressed in the S and G2 phases. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days. Observed in late guard mother cells (GMC), newly formed guard cells, and immature stomata, thus being expressed just before and after the symmetric division of stomatal differentiation (PubMed:20675570).4 Publications

Inductioni

Repressed by MYB88 and MYB124 during stomatal development.1 Publication

Gene expression databases

ExpressionAtlasiP25859 baseline and differential
GenevisibleiP25859 AT

Interactioni

Subunit structurei

Interacts with CKS1 (PubMed:9276444, PubMed:11319029). Interacts with CYCU3-1 (PubMed:15197472). Interacts with SIM, SMR1 and SMR2 (PubMed:20706207).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CKS1O232497EBI-1253311,EBI-1253127

GO - Molecular functioni

Protein-protein interaction databases

BioGridi9902, 50 interactors
IntActiP25859, 25 interactors
STRINGi3702.AT3G54180.1

Structurei

3D structure databases

ProteinModelPortaliP25859
SMRiP25859
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 301Protein kinasePROSITE-ProRule annotationAdd BLAST298

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
HOGENOMiHOG000233024
InParanoidiP25859
KOiK07760
OMAiGKYTHEV
OrthoDBiEOG09360F64
PhylomeDBiP25859

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P25859-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKYEKLEKV GEGTYGKVYK AMEKGTGKLV ALKKTRLEMD EEGIPPTALR
60 70 80 90 100
EISLLQMLST SIYVVRLLCV EHVHQPSTKS QSTKSNLYLV FEYLDTDLKK
110 120 130 140 150
FIDSYRKGPN PKPLEPFLIQ KLMFQLCKGV AHCHSHGVLH RDLKPQNLLL
160 170 180 190 200
VKDKELLKIA DLGLGRAFTV PLKSYTHEIV TLWYRAPEVL LGSTHYSTGV
210 220 230 240 250
DMWSVGCIFA EMVRRQALFP GDSEFQQLLH IFRLLGTPTE QQWPGVSTLR
260 270 280 290 300
DWHVYPKWEP QDLTLAVPSL SPQGVDLLTK MLKYNPAERI SAKTALDHPY

FDSLDKSQF
Length:309
Mass (Da):35,318
Last modified:November 1, 1995 - v2
Checksum:i8FFCD006A9A4A75B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti226Q → R in CAA40972 (PubMed:1937013).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10851 Genomic DNA Translation: BAA01624.1
AL132957 Genomic DNA Translation: CAB70992.1
CP002686 Genomic DNA Translation: AEE79196.1
BT026079 mRNA Translation: ABG48435.1
AY084810 mRNA Translation: AAM61376.1
X57840 mRNA Translation: CAA40972.1
PIRiS23096
RefSeqiNP_190986.1, NM_115278.3
UniGeneiAt.10

Genome annotation databases

EnsemblPlantsiAT3G54180.1; AT3G54180.1; AT3G54180
GeneIDi824585
GrameneiAT3G54180.1; AT3G54180.1; AT3G54180
KEGGiath:AT3G54180

Similar proteinsi

Entry informationi

Entry nameiCKB11_ARATH
AccessioniPrimary (citable) accession number: P25859
Secondary accession number(s): Q147L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1995
Last modified: September 12, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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