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UniProtKB - P25853 (BAM5_ARATH)
Protein
Beta-amylase 5
Gene
BAM5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Beta-amylase activity. Major cytosolic beta-amylase isoform in rosette leaves and inflorescences stems.
1 PublicationCatalytic activityi
- Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains. EC:3.2.1.2
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 56 | SubstrateBy similarity | 1 | |
Binding sitei | 96 | SubstrateBy similarity | 1 | |
Binding sitei | 104 | SubstrateBy similarity | 1 | |
Active sitei | 189 | Proton donorPROSITE-ProRule annotation | 1 | |
Binding sitei | 298 | SubstrateBy similarity | 1 | |
Binding sitei | 303 | SubstrateBy similarity | 1 | |
Binding sitei | 345 | SubstrateBy similarity | 1 | |
Active sitei | 383 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 423 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- amylopectin maltohydrolase activity Source: UniProtKB-EC
- beta-amylase activity Source: TAIR
GO - Biological processi
- response to herbivore Source: TAIR
- starch catabolic process Source: TAIR
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Polysaccharide degradation |
Protein family/group databases
CAZyi | GH14, Glycoside Hydrolase Family 14 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-amylase 5 (EC:3.2.1.2)Short name: AtBeta-Amy Alternative name(s): 1,4-alpha-D-glucan maltohydrolase Protein REDUCED BETA AMYLASE 1 |
Gene namesi | Name:BAM5 Synonyms:BMY1, RAM1 Ordered Locus Names:At4g15210 ORF Names:dl3650c, FCAALL.97 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G15210 |
TAIRi | locus:2129810, AT4G15210 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Present in the continuous phloem cytoplasm.
Other locations
- secretory vesicle Source: TAIR
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Almost complete loss of beta-amylase activity in rosette leaves and inflorescences (stems).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153932 | 1 – 498 | Beta-amylase 5Add BLAST | 498 |
Proteomic databases
PaxDbi | P25853 |
PRIDEi | P25853 |
ProteomicsDBi | 241124 [P25853-1] |
PTM databases
iPTMneti | P25853 |
Expressioni
Tissue specificityi
Detected in phloem sieve elements.1 Publication
Inductioni
Circadian-regulated, with a peak in expression just before the light period in short day conditions.
Gene expression databases
ExpressionAtlasi | P25853, baseline and differential |
Genevisiblei | P25853, AT |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 384 – 385 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 14 family.Curated
Phylogenomic databases
eggNOGi | ENOG502QUU5, Eukaryota |
InParanoidi | P25853 |
OMAi | PFPWDEE |
OrthoDBi | 533202at2759 |
PhylomeDBi | P25853 |
Family and domain databases
InterProi | View protein in InterPro IPR001554, Glyco_hydro_14 IPR018238, Glyco_hydro_14_CS IPR001371, Glyco_hydro_14B_pln IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR31352, PTHR31352, 1 hit |
Pfami | View protein in Pfam PF01373, Glyco_hydro_14, 1 hit |
PRINTSi | PR00750, BETAAMYLASE PR00842, GLHYDLASE14B |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00506, BETA_AMYLASE_1, 1 hit PS00679, BETA_AMYLASE_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P25853-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATNYNEKLL LNYVPVYVML PLGVVNVENV FADPETLETQ LKRLKEEAGV
60 70 80 90 100
DGVMVDVWWG IIESKGPKQY DWTAYKTLFQ LIARLGLKIQ AIMSFHQCGG
110 120 130 140 150
NVGDIVTIPI PQWVRDVGDN DPDIYYTNRK GTRDIEYLSI GVDNLPLFAG
160 170 180 190 200
RTAVQLYSDY MSSFKENMAD LIEAGVIVDI EVGLGPAGEL RYPSYPQSQG
210 220 230 240 250
WVFPGIGEFQ CYDKYLKKDF KEAAAKAGHP EWDLPEDAGE YNDKPEETGF
260 270 280 290 300
FKKDGTYVSE KGKFFMTWYS NKLIFHGDQI LGEANKIFAG LKVNLAAKVS
310 320 330 340 350
GIHWLYNHHS HAAELTAGYY NLFKRDGYRP IARMLSKHYG ILNFTCLEMK
360 370 380 390 400
DTDNTAEALS APQELVQEVL SKAWKEGIEV AGENALETYG AKGYNQILLN
410 420 430 440 450
ARPNGVNPNG KPKLRMYGFT YLRLSDTVFQ ENNFELFKKL VRKMHADQDY
460 470 480 490
CGDAAKYGHE IVPLKTSNSQ LTLEDIADAA QPSGAFKWDS ETDLKVDG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8B7H0 | A0A1P8B7H0_ARATH | Beta-amylase | BAM5 AT-BETA-AMY, ATBETA-AMY, beta-amylase 5, BMY1, RAM1 | 445 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 155 | Q → QV in CAB10300 (PubMed:9461215).Curated | 1 | |
Sequence conflicti | 155 | Q → QV in CAB78563 (PubMed:10617198).Curated | 1 | |
Sequence conflicti | 486 | F → L in BAA07842 (PubMed:7716246).Curated | 1 | |
Sequence conflicti | 486 | F → L in AAB34026 (PubMed:7716246).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038978 | 411 – 420 | KPKLRMYGFT → LLWRRSEVRA in isoform 2. 1 Publication | 10 | |
Alternative sequenceiVSP_038979 | 421 – 498 | Missing in isoform 2. 1 PublicationAdd BLAST | 78 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73467 mRNA Translation: AAA32737.1 D43783 Genomic DNA Translation: BAA07842.1 S77076 Genomic DNA Translation: AAB34026.1 Z97338 Genomic DNA Translation: CAB10300.1 AL161540 Genomic DNA Translation: CAB78563.1 CP002687 Genomic DNA Translation: AEE83568.1 CP002687 Genomic DNA Translation: AEE83569.1 AF424573 mRNA Translation: AAL11567.1 AY142024 mRNA Translation: AAM98288.1 AK316869 mRNA Translation: BAH19577.1 AK226353 mRNA Translation: BAE98501.1 |
PIRi | B71416, S36094 |
RefSeqi | NP_567460.1, NM_117609.3 [P25853-1] NP_849389.1, NM_179058.1 [P25853-2] |
Genome annotation databases
EnsemblPlantsi | AT4G15210.1; AT4G15210.1; AT4G15210 [P25853-1] AT4G15210.2; AT4G15210.2; AT4G15210 [P25853-2] |
GeneIDi | 827185 |
Gramenei | AT4G15210.1; AT4G15210.1; AT4G15210 [P25853-1] AT4G15210.2; AT4G15210.2; AT4G15210 [P25853-2] |
KEGGi | ath:AT4G15210 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73467 mRNA Translation: AAA32737.1 D43783 Genomic DNA Translation: BAA07842.1 S77076 Genomic DNA Translation: AAB34026.1 Z97338 Genomic DNA Translation: CAB10300.1 AL161540 Genomic DNA Translation: CAB78563.1 CP002687 Genomic DNA Translation: AEE83568.1 CP002687 Genomic DNA Translation: AEE83569.1 AF424573 mRNA Translation: AAL11567.1 AY142024 mRNA Translation: AAM98288.1 AK316869 mRNA Translation: BAH19577.1 AK226353 mRNA Translation: BAE98501.1 |
PIRi | B71416, S36094 |
RefSeqi | NP_567460.1, NM_117609.3 [P25853-1] NP_849389.1, NM_179058.1 [P25853-2] |
3D structure databases
SMRi | P25853 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT4G15210.1 |
Protein family/group databases
CAZyi | GH14, Glycoside Hydrolase Family 14 |
PTM databases
iPTMneti | P25853 |
Proteomic databases
PaxDbi | P25853 |
PRIDEi | P25853 |
ProteomicsDBi | 241124 [P25853-1] |
Genome annotation databases
EnsemblPlantsi | AT4G15210.1; AT4G15210.1; AT4G15210 [P25853-1] AT4G15210.2; AT4G15210.2; AT4G15210 [P25853-2] |
GeneIDi | 827185 |
Gramenei | AT4G15210.1; AT4G15210.1; AT4G15210 [P25853-1] AT4G15210.2; AT4G15210.2; AT4G15210 [P25853-2] |
KEGGi | ath:AT4G15210 |
Organism-specific databases
Araporti | AT4G15210 |
TAIRi | locus:2129810, AT4G15210 |
Phylogenomic databases
eggNOGi | ENOG502QUU5, Eukaryota |
InParanoidi | P25853 |
OMAi | PFPWDEE |
OrthoDBi | 533202at2759 |
PhylomeDBi | P25853 |
Miscellaneous databases
PROi | PR:P25853 |
Gene expression databases
ExpressionAtlasi | P25853, baseline and differential |
Genevisiblei | P25853, AT |
Family and domain databases
InterProi | View protein in InterPro IPR001554, Glyco_hydro_14 IPR018238, Glyco_hydro_14_CS IPR001371, Glyco_hydro_14B_pln IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR31352, PTHR31352, 1 hit |
Pfami | View protein in Pfam PF01373, Glyco_hydro_14, 1 hit |
PRINTSi | PR00750, BETAAMYLASE PR00842, GLHYDLASE14B |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00506, BETA_AMYLASE_1, 1 hit PS00679, BETA_AMYLASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BAM5_ARATH | |
Accessioni | P25853Primary (citable) accession number: P25853 Secondary accession number(s): O23375, Q0WWJ7, Q3EA19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families