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Entry version 181 (12 Aug 2020)
Sequence version 2 (19 Sep 2003)
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Protein

Maternal protein pumilio

Gene

pum

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:1459455, PubMed:1576962, PubMed:9404893, PubMed:9660969, PubMed:22345517). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of deadenylase complexes leading to translational inhibition and mRNA degradation (By similarity). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:22345517). Mediates post-transcriptional silencing of E2f mRNA by binding to its 3'-UTR and promoting miRNA regulation (PubMed:22345517). Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole (PubMed:1459455, PubMed:1576962, PubMed:9404893, PubMed:9660969).By similarity5 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDevelopmental protein, Repressor, RNA-binding
Biological processTranslation regulation

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-DME-432722, Golgi Associated Vesicle Biogenesis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Maternal protein pumilio
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pum
ORF Names:CG9755
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3R

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0003165, pum

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Flies display defective abdomen pattern formation and are embryonic lethal.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi1127R → A: Disrupts RNA-binding. 1 Publication1
Mutagenesisi1167K → A: Disrupts RNA-binding. 1 Publication1
Mutagenesisi1199R → A: Disrupts RNA-binding. 1 Publication1
Mutagenesisi1235H → A: Disrupts RNA-binding. 1 Publication1
Mutagenesisi1330G → D in Pum680; abolishes interaction with brat and translational repression activity but not RNA-binding activity. 2 Publications1
Mutagenesisi1346E → K: Disrupts RNA-binding. 1 Publication1
Mutagenesisi1365C → R: Abolishes interaction with brat. 1 Publication1
Mutagenesisi1366T → D: Abolishes interaction with brat. 1 Publication1
Mutagenesisi1367F → S: Abolishes interaction with nos. 1 Publication1
Mutagenesisi1368N → S: Abolishes interaction with brat. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000759161 – 1533Maternal protein pumilioAdd BLAST1533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei453Phosphoserine1 Publication1
Modified residuei468Phosphoserine1 Publication1
Modified residuei470Phosphoserine1 Publication1
Modified residuei477Phosphoserine1 Publication1
Modified residuei505Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P25822

PRoteomics IDEntifications database

More...
PRIDEi
P25822

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P25822

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed both maternally and zygotically in embryos.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0003165, Expressed in embryonic/larval hemocyte (Drosophila) and 42 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
P25822, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
P25822, DM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with nos and brat. Acts via the formation of a quaternary complex composed of pum, nos, brat and the 3'-UTR mRNA of hb.

2 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
66261, 53 interactors

Protein interaction database and analysis system

More...
IntActi
P25822, 4 interactors

Molecular INTeraction database

More...
MINTi
P25822

STRING: functional protein association networks

More...
STRINGi
7227.FBpp0305823

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11533
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P25822

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P25822

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1091 – 1428PUM-HDPROSITE-ProRule annotationAdd BLAST338
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati1111 – 1146Pumilio 1Add BLAST36
Repeati1147 – 1182Pumilio 2Add BLAST36
Repeati1183 – 1218Pumilio 3Add BLAST36
Repeati1219 – 1254Pumilio 4Add BLAST36
Repeati1255 – 1290Pumilio 5Add BLAST36
Repeati1291 – 1326Pumilio 6Add BLAST36
Repeati1327 – 1362Pumilio 7Add BLAST36
Repeati1366 – 1402Pumilio 8Add BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1126 – 1130Adenine-nucleotide binding in RNA targetBy similarity5
Regioni1162 – 1166Uracil-nucleotide binding in RNA targetBy similarity5
Regioni1198 – 1202Adenine-nucleotide binding in RNA targetBy similarity5
Regioni1234 – 1238Non-specific-nucleotide binding in RNA targetBy similarity5
Regioni1270 – 1274Adenine-nucleotide binding in RNA targetBy similarity5
Regioni1306 – 1310Uracil-nucleotide binding in RNA targetBy similarity5
Regioni1342 – 1346Guanine-nucleotide binding in RNA targetBy similarity5
Regioni1382 – 1386Uracil-nucleotide binding in RNA targetBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi34 – 45Ala-richAdd BLAST12
Compositional biasi57 – 77Gly/Val-richAdd BLAST21
Compositional biasi83 – 93Ala-richAdd BLAST11
Compositional biasi130 – 174Gly-richAdd BLAST45
Compositional biasi152 – 164Poly-GlyAdd BLAST13
Compositional biasi181 – 212Ala-richAdd BLAST32
Compositional biasi213 – 236Gln-richAdd BLAST24
Compositional biasi262 – 274Poly-GlnAdd BLAST13
Compositional biasi571 – 599Gly-richAdd BLAST29
Compositional biasi708 – 725Poly-GlnAdd BLAST18
Compositional biasi936 – 946Poly-AlaAdd BLAST11
Compositional biasi1050 – 1062Poly-AlaAdd BLAST13

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. RNA-binding occurs on the concave side of the surface. Pum is composed of 8 pumilio repeats of 36 residues; each repeat binds a single nucleotide in its RNA target. Residues at positions 12 and 16 of the pumilio repeat bind each RNA base via hydrogen bonding or van der Waals contacts with the Watson-Crick edge, while the amino acid at position 13 makes a stacking interaction. The recognition of RNA by pumilio repeats is base specific: cysteine and glutamine at position 12 and 16, respectively, bind adenine; asparagine and glutamine bind uracil; and serine and glutamate bind guanine.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1488, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000169170

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_004017_0_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P25822

KEGG Orthology (KO)

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KOi
K17943

Identification of Orthologs from Complete Genome Data

More...
OMAi
MAGGDSM

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P25822

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07920, Pumilio, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.25.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR033133, PUM-HD
IPR033712, Pumilio_RNA-bd
IPR001313, Pumilio_RNA-bd_rpt

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00806, PUF, 8 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00025, Pumilio, 8 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48371, SSF48371, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50302, PUM, 8 hits
PS50303, PUM_HD, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform A (identifier: P25822-1) [UniParc]FASTAAdd to basket
Also known as: C, D

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKFLGGNDDR NGRGGVGVGT DAIVGSRGGV SQDAADAAGA AAAAAVGYVF
60 70 80 90 100
QQRPSPGGVG VGVGGVGGGV PGVGAVGSTL HEAAAAEYAA HFAQKQQQTR
110 120 130 140 150
WACGDDGHGI DNPDKWKYNP PMNPANAAPG GPPGNGSNGG PGAIGTIGMG
160 170 180 190 200
SGLGGGGGGG AGGGNNGGSG TNGGLHHQSM AAAAANMAAM QQAAALAKHN
210 220 230 240 250
HMISQAAAAV AAQQQHQHPH QQHPQQQQQQ QQAQNQGHPH HLMGGGNGLG
260 270 280 290 300
NGNGLGIQHP GQQQQQQQQQ QQQQHPGQYN ANLLNHAAAL GHMSSYAQSG
310 320 330 340 350
GSMYDHHGGA MHPGMNGGMP KQQPLGPPGA GGPQDYVYMG GQTTVPMGAA
360 370 380 390 400
MMPPQNQYMN SSAVAAANRN AAITTSTAKK LWEKSDGKGV SSSTPGGPLH
410 420 430 440 450
PLQIPGIGDP SSVWKDHTWS TQGENILVPP PSRAYAHGGA SDTSNSGNAG
460 470 480 490 500
ILSPRDSTCA KVVEYVFSGS PTNKDSSLSG LEPHLRNLKF DDNDKSRDDK
510 520 530 540 550
EKANSPFDTN GLKKDDQVTN SNGVVNGIDD DKGFNRTPGS RQPSPAEESQ
560 570 580 590 600
PRPPNLLFPP LPFNHMLMDH GQGMGGGLGG VVGSGNGVGG GSGGGGAGGA
610 620 630 640 650
YAAHQQMAAQ MSQLQPPMMN GVGGGMPMAA QSPMLNHQAA GPNHMESPGN
660 670 680 690 700
LLQQQNFDVQ QLFRSQNPGL AAVATNAAAA AAAAAAATSA ASAAAAVGAP
710 720 730 740 750
PVPNGSLQQS QQQQQQQQQQ QQQQQMHMAA ASQQFLAAQQ QAQNAAYAAQ
760 770 780 790 800
QATSYVINPG QEAAPYMGMI AAAQMPYYGV APWGMYPGNL IPQQGTQPRR
810 820 830 840 850
PLTPSQQGAE NQPYQVIPAF LDHTGSLLMG GPRTGTPMRL VSPAPVLVPP
860 870 880 890 900
GATRAGPPPP QGPQLYQPQP QTAQQNLYSQ QNGSSVGGLA LNTSSLTGRR
910 920 930 940 950
DSFDRSTSAF SPSTMDYTSS GVAAAANAVN STVAQAAAAA AAAAAARGKW
960 970 980 990 1000
PGAMSGAASG AYGALGAGNA SASPLGAPIT PPPSAQSCLL GSRAPGAESR
1010 1020 1030 1040 1050
QRQQQQQQLA AVGLPATAAA AQAAVAAAAN NMFGSNSSIF SNPLAIPGTA
1060 1070 1080 1090 1100
AVAAAAAAAA AANSRQVAAT AAAAAAVAAA AGGVGGAPQP GRSRLLEDFR
1110 1120 1130 1140 1150
NQRYPNLQLR DLANHIVEFS QDQHGSRFIQ QKLERATAAE KQMVFSEILA
1160 1170 1180 1190 1200
AAYSLMTDVF GNYVIQKFFE FGTPEQKNTL GMQVKGHVLQ LALQMYGCRV
1210 1220 1230 1240 1250
IQKALESISP EQQQEIVHEL DGHVLKCVKD QNGNHVVQKC IECVDPVALQ
1260 1270 1280 1290 1300
FIINAFKGQV YSLSTHPYGC RVIQRILEHC TAEQTTPILD ELHEHTEQLI
1310 1320 1330 1340 1350
QDQYGNYVIQ HVLEHGKQED KSILINSVRG KVLVLSQHKF ASNVVEKCVT
1360 1370 1380 1390 1400
HATRGERTGL IDEVCTFNDN ALHVMMKDQY ANYVVQKMID VSEPTQLKKL
1410 1420 1430 1440 1450
MTKIRPHMAA LRKYTYGKHI NAKLEKYYMK ITNPITVGTG AGGVPAASSA
1460 1470 1480 1490 1500
AAVSSGATSA SVTACTSGSS TTTTSTTNSL ASPTICSVQE NGSAMVVEPS
1510 1520 1530
SPDASESSSS VVSGAVNSSL GPIGPPTNGN VVL
Length:1,533
Mass (Da):157,528
Last modified:September 19, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0A343220BB1F0B27
GO
Isoform B (identifier: P25822-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-348: Missing.
     349-372: AAMMPPQNQYMNSSAVAAANRNAA → MMKLLHDFILDARTAEDVALTQEM

Show »
Length:1,185
Mass (Da):123,520
Checksum:i850A3AEDEC837AD0
GO
Isoform E (identifier: P25822-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-598: Missing.
     599-659: GAYAAHQQMA...NLLQQQNFDV → MVVLETASAL...DVLSLAFNHN

Show »
Length:935
Mass (Da):97,754
Checksum:i22ECEC73646072EF
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0B4KFF0A0A0B4KFF0_DROME
Pumilio, isoform G
pum anon-WO0172774.19, bem, CG9763, Dmel\CG9755, EP(3)0883
1,533Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0B4K612A0A0B4K612_DROME
Pumilio, isoform F
pum anon-WO0172774.19, bem, CG9763, Dmel\CG9755, EP(3)0883
925Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0B4KFX7A0A0B4KFX7_DROME
Pumilio, isoform H
pum anon-WO0172774.19, bem, CG9763, Dmel\CG9755, EP(3)0883
921Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAX33465 differs from that shown. Reason: Frameshift.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti362S → A in CAA44474 (PubMed:1576962).Curated1
Sequence conflicti1103R → P in AAB59189 (PubMed:1459455).Curated1
Sequence conflicti1165I → S in AAX33465 (Ref. 6) Curated1
Sequence conflicti1374V → M in AAQ22439 (PubMed:12537569).Curated1
Sequence conflicti1406 – 1407PH → KN in CAA44474 (PubMed:1576962).Curated2
Sequence conflicti1491N → S in AAQ22439 (PubMed:12537569).Curated1
Sequence conflicti1496V → I in AAB59189 (PubMed:1459455).Curated1
Sequence conflicti1519S → G in AAB59189 (PubMed:1459455).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0093131 – 598Missing in isoform E. 1 PublicationAdd BLAST598
Alternative sequenceiVSP_0082161 – 348Missing in isoform B. CuratedAdd BLAST348
Alternative sequenceiVSP_008217349 – 372AAMMP…NRNAA → MMKLLHDFILDARTAEDVAL TQEM in isoform B. CuratedAdd BLAST24
Alternative sequenceiVSP_009314599 – 659GAYAA…QNFDV → MVVLETASALLGGPYAQGAP ALKMVQKRYIGLHHWLGPIR SKELKEHIVSDDVLSLAFNH N in isoform E. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
X62589 mRNA Translation: CAA44474.1
L07943 mRNA Translation: AAB59189.1
AE014297 Genomic DNA Translation: AAF54340.2
AE014297 Genomic DNA Translation: AAF54338.2
AE014297 Genomic DNA Translation: AAN13409.1
AE014297 Genomic DNA Translation: AAN13410.1
AE014297 Genomic DNA Translation: AAO41523.1
BT001872 mRNA Translation: AAN71644.1
BT009970 mRNA Translation: AAQ22439.1
BT021317 mRNA Translation: AAX33465.1 Frameshift.

Protein sequence database of the Protein Information Resource

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PIRi
A46221

NCBI Reference Sequences

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RefSeqi
NP_001262403.1, NM_001275474.1 [P25822-1]
NP_524285.2, NM_079561.4 [P25822-1]
NP_731314.1, NM_169258.3 [P25822-1]
NP_731315.1, NM_169259.3 [P25822-1]
NP_731316.2, NM_169260.4 [P25822-2]
NP_788604.1, NM_176427.3 [P25822-3]

Genome annotation databases

Ensembl metazoan genome annotation project

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EnsemblMetazoai
FBtr0081990; FBpp0081470; FBgn0003165 [P25822-1]
FBtr0081991; FBpp0081471; FBgn0003165 [P25822-1]
FBtr0081992; FBpp0081472; FBgn0003165 [P25822-1]
FBtr0081993; FBpp0081473; FBgn0003165 [P25822-2]
FBtr0081994; FBpp0081474; FBgn0003165 [P25822-3]
FBtr0333667; FBpp0305823; FBgn0003165 [P25822-1]

Database of genes from NCBI RefSeq genomes

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GeneIDi
41094

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
dme:Dmel_CG9755

UCSC genome browser

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UCSCi
CG9755-RA, d. melanogaster [P25822-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62589 mRNA Translation: CAA44474.1
L07943 mRNA Translation: AAB59189.1
AE014297 Genomic DNA Translation: AAF54340.2
AE014297 Genomic DNA Translation: AAF54338.2
AE014297 Genomic DNA Translation: AAN13409.1
AE014297 Genomic DNA Translation: AAN13410.1
AE014297 Genomic DNA Translation: AAO41523.1
BT001872 mRNA Translation: AAN71644.1
BT009970 mRNA Translation: AAQ22439.1
BT021317 mRNA Translation: AAX33465.1 Frameshift.
PIRiA46221
RefSeqiNP_001262403.1, NM_001275474.1 [P25822-1]
NP_524285.2, NM_079561.4 [P25822-1]
NP_731314.1, NM_169258.3 [P25822-1]
NP_731315.1, NM_169259.3 [P25822-1]
NP_731316.2, NM_169260.4 [P25822-2]
NP_788604.1, NM_176427.3 [P25822-3]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H3DX-ray2.30X/Y1093-1411[»]
5KL1X-ray3.70A1091-1426[»]
5KL8X-ray4.00A1091-1426[»]
5KLAX-ray1.14A1091-1426[»]
SMRiP25822
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi66261, 53 interactors
IntActiP25822, 4 interactors
MINTiP25822
STRINGi7227.FBpp0305823

PTM databases

iPTMnetiP25822

Proteomic databases

PaxDbiP25822
PRIDEiP25822

Genome annotation databases

EnsemblMetazoaiFBtr0081990; FBpp0081470; FBgn0003165 [P25822-1]
FBtr0081991; FBpp0081471; FBgn0003165 [P25822-1]
FBtr0081992; FBpp0081472; FBgn0003165 [P25822-1]
FBtr0081993; FBpp0081473; FBgn0003165 [P25822-2]
FBtr0081994; FBpp0081474; FBgn0003165 [P25822-3]
FBtr0333667; FBpp0305823; FBgn0003165 [P25822-1]
GeneIDi41094
KEGGidme:Dmel_CG9755
UCSCiCG9755-RA, d. melanogaster [P25822-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
41094
FlyBaseiFBgn0003165, pum

Phylogenomic databases

eggNOGiKOG1488, Eukaryota
GeneTreeiENSGT00940000169170
HOGENOMiCLU_004017_0_1_1
InParanoidiP25822
KOiK17943
OMAiMAGGDSM
PhylomeDBiP25822

Enzyme and pathway databases

ReactomeiR-DME-432722, Golgi Associated Vesicle Biogenesis

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
41094, 1 hit in 5 CRISPR screens
EvolutionaryTraceiP25822

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
41094

Protein Ontology

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PROi
PR:P25822

Gene expression databases

BgeeiFBgn0003165, Expressed in embryonic/larval hemocyte (Drosophila) and 42 other tissues
ExpressionAtlasiP25822, baseline and differential
GenevisibleiP25822, DM

Family and domain databases

CDDicd07920, Pumilio, 1 hit
Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR033133, PUM-HD
IPR033712, Pumilio_RNA-bd
IPR001313, Pumilio_RNA-bd_rpt
PfamiView protein in Pfam
PF00806, PUF, 8 hits
SMARTiView protein in SMART
SM00025, Pumilio, 8 hits
SUPFAMiSSF48371, SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50302, PUM, 8 hits
PS50303, PUM_HD, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPUM_DROME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P25822
Secondary accession number(s): A4V2K5
, Q5BIA7, Q7YU65, Q8IGA8, Q9VHH4, Q9VHH6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 19, 2003
Last modified: August 12, 2020
This is version 181 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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