UniProtKB - P25808 (SPB4_YEAST)
ATP-dependent rRNA helicase SPB4
SPB4
Functioni
ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits (PubMed:2408148, PubMed:25579579).
Binds 90S pre-ribosomal particles and dissociates from pre-60S ribosomal particles after processing of 27SB pre-rRNA (PubMed:21825077).
Required for the normal formation of 18S rRNA through the processing of pre-rRNAs at sites A0, A1 and A2, and the normal formation of 25S and 5.8S rRNAs through the processing of pre-rRNAs at sites C1 and C2 (PubMed:9769101).
Also required for recruitment of NOG2 to pre-ribosomes (PubMed:22735702).
5 PublicationsMiscellaneous
Catalytic activityi
- EC:3.6.4.131 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 51 – 58 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- RNA binding Source: UniProtKB-KW
- RNA helicase activity Source: SGD
GO - Biological processi
- assembly of large subunit precursor of preribosome Source: SGD
- maturation of LSU-rRNA Source: SGD
- ribosomal large subunit assembly Source: SGD
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Ribosome biogenesis, rRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent rRNA helicase SPB4Curated (EC:3.6.4.131 Publication)Alternative name(s): Suppressor of PAB1 protein 41 Publication |
Gene namesi | Name:SPB41 Publication Ordered Locus Names:YFL002CImported |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001894, SPB4 |
VEuPathDBi | FungiDB:YFL002C |
Subcellular locationi
Nucleus
- nucleolus 2 Publications
Nucleus
- nucleolus Source: SGD
- nucleoplasm Source: SGD
Other locations
- 90S preribosome Source: SGD
- preribosome, large subunit precursor Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 360 | R → G: Leads to accumulation of 35S and 27SB pre-rRNAs and a net 40S ribosomal subunit defect. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055065 | 1 – 606 | ATP-dependent rRNA helicase SPB4Add BLAST | 606 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 254 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P25808 |
PaxDbi | P25808 |
PRIDEi | P25808 |
PTM databases
iPTMneti | P25808 |
Interactioni
Subunit structurei
Component of pre-60S ribosomal complexes.
4 PublicationsProtein-protein interaction databases
BioGRIDi | 31147, 681 interactors |
DIPi | DIP-5423N |
IntActi | P25808, 36 interactors |
MINTi | P25808 |
STRINGi | 4932.YFL002C |
Miscellaneous databases
RNActi | P25808, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 224 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 187 | |
Domaini | 248 – 404 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 157 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 539 – 582 | Sequence analysisAdd BLAST | 44 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 7 – 35 | Q motifCuratedAdd BLAST | 29 | |
Motifi | 172 – 175 | DEAD boxCurated | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0345, Eukaryota |
GeneTreei | ENSGT00550000074969 |
HOGENOMi | CLU_003041_26_4_1 |
InParanoidi | P25808 |
OMAi | CIVYFPT |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR025313, DUF4217 IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF13959, DUF4217, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM01178, DUF4217, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKSLEWDNL GFSLLPWIRT GLDVMGFETM TPVQASTIPM LAGNKDVVVD
60 70 80 90 100
SVTGSGKTAA FVIPVLEKVV KEEANTSKFK KAHFHSLIIA PTRELSRQIE
110 120 130 140 150
SVVLSFLEHY PSDLFPIKCQ LLVGTNEATV RDDVSNFLRN RPQILIGTPG
160 170 180 190 200
RVLDFLQMPA VKTSACSMVV MDEADRLLDM SFIKDTEKIL RLLPKQRRTG
210 220 230 240 250
LFSATMRSAG SDIFKTGLRN PVRITVNSKN QAPSSLKLNY CVVNPAEKLQ
260 270 280 290 300
LLVSILNNYK FKKCIVYFPT CVSVSYFYSF IQYLGKRNIL VNEVEIFSLH
310 320 330 340 350
GKLQTSARTK TLTAFTDSLS NSVLFTTDVA ARGIDIPDVD LVIQLDPPTN
360 370 380 390 400
TDMFMHRCGR TGRANRVGKA ITFLNEGREE DFIPFMQVKN VELEELDLEV
410 420 430 440 450
KGITTNFYED FRNWILEDRD RFDKGVKAYV AFIKYYSNHS ATSIFRLQSL
460 470 480 490 500
DYVGIAKLYG LFRLPRMPEI TKYLATEKQE GIFPGNWLVD PPVNMDEYKY
510 520 530 540 550
KDKKREKERQ ETLKNISLIN DKKKLKSELK KKNLAWSDKT LTKERKLERK
560 570 580 590 600
EKMSLKRKAI EEELKAEELD ENAEEERIKE DWKEIVLQNK RKKVSSKAIQ
GNFDDL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16147 Genomic DNA Translation: CAA34272.1 D50617 Genomic DNA Translation: BAA09238.1 BK006940 Genomic DNA Translation: DAA12439.1 |
PIRi | S14942 |
RefSeqi | NP_116654.1, NM_001179964.1 |
Genome annotation databases
EnsemblFungii | YFL002C_mRNA; YFL002C; YFL002C |
GeneIDi | 850549 |
KEGGi | sce:YFL002C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16147 Genomic DNA Translation: CAA34272.1 D50617 Genomic DNA Translation: BAA09238.1 BK006940 Genomic DNA Translation: DAA12439.1 |
PIRi | S14942 |
RefSeqi | NP_116654.1, NM_001179964.1 |
3D structure databases
SMRi | P25808 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31147, 681 interactors |
DIPi | DIP-5423N |
IntActi | P25808, 36 interactors |
MINTi | P25808 |
STRINGi | 4932.YFL002C |
PTM databases
iPTMneti | P25808 |
Proteomic databases
MaxQBi | P25808 |
PaxDbi | P25808 |
PRIDEi | P25808 |
Genome annotation databases
EnsemblFungii | YFL002C_mRNA; YFL002C; YFL002C |
GeneIDi | 850549 |
KEGGi | sce:YFL002C |
Organism-specific databases
SGDi | S000001894, SPB4 |
VEuPathDBi | FungiDB:YFL002C |
Phylogenomic databases
eggNOGi | KOG0345, Eukaryota |
GeneTreei | ENSGT00550000074969 |
HOGENOMi | CLU_003041_26_4_1 |
InParanoidi | P25808 |
OMAi | CIVYFPT |
Miscellaneous databases
PROi | PR:P25808 |
RNActi | P25808, protein |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR025313, DUF4217 IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF13959, DUF4217, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM01178, DUF4217, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPB4_YEAST | |
Accessioni | P25808Primary (citable) accession number: P25808 Secondary accession number(s): D6VTM9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - SIMILARITY comments
Index of protein domains and families