UniProtKB - P25798 (FLIF_ECOLI)
Protein
Flagellar M-ring protein
Gene
fliF
Organism
Escherichia coli (strain K12)
Status
Functioni
The M ring may be actively involved in energy transduction.
GO - Molecular functioni
- motor activity Source: InterPro
GO - Biological processi
- bacterial-type flagellum-dependent cell motility Source: InterPro
Enzyme and pathway databases
BioCyci | EcoCyc:FLIF-FLAGELLAR-MS-RING |
Names & Taxonomyi
Protein namesi | Recommended name: Flagellar M-ring protein |
Gene namesi | Name:fliF Synonyms:fla AII.1, fla BI Ordered Locus Names:b1938, JW1922 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
- Bacterial flagellum basal body 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 23 – 43 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 447 – 467 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- bacterial-type flagellum basal body, MS ring Source: InterPro
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Bacterial flagellum, Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180882 | 1 – 552 | Flagellar M-ring proteinAdd BLAST | 552 |
Proteomic databases
PaxDbi | P25798 |
PRIDEi | P25798 |
Interactioni
Subunit structurei
The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The M ring is integral to the inner membrane of the cell and may be connected to the flagellar rod via the S ring. The S (supramembrane ring) lies just distal to the M ring. The L and P rings lie in the outer membrane and the periplasmic space, respectively.
Binary interactionsi
P25798
With | #Exp. | IntAct |
---|---|---|
fliG [P0ABZ1] | 4 | EBI-1126492,EBI-1126524 |
Protein-protein interaction databases
BioGRIDi | 4260383, 20 interactors 850802, 1 interactor |
DIPi | DIP-401N |
IntActi | P25798, 4 interactors |
STRINGi | 511145.b1938 |
Family & Domainsi
Sequence similaritiesi
Belongs to the FliF family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1766, Bacteria |
HOGENOMi | CLU_028108_1_0_6 |
InParanoidi | P25798 |
PhylomeDBi | P25798 |
Family and domain databases
InterProi | View protein in InterPro IPR013556, Flag_M-ring_C IPR000067, FlgMring_FliF IPR006182, FliF_N_dom IPR043427, YscJ/FliF |
PANTHERi | PTHR30046, PTHR30046, 1 hit PTHR30046:SF0, PTHR30046:SF0, 1 hit |
Pfami | View protein in Pfam PF01514, YscJ_FliF, 1 hit PF08345, YscJ_FliF_C, 1 hit |
PIRSFi | PIRSF004862, FliF, 1 hit |
PRINTSi | PR01009, FLGMRINGFLIF |
TIGRFAMsi | TIGR00206, fliF, 1 hit |
i Sequence
Sequence statusi: Complete.
P25798-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNATAAQTKS LEWLNRLRAN PKIPLIVAGS AAVAVMVALI LWAKAPDYRT
60 70 80 90 100
LFSNLSDQDG GAIVSQLTQM NIPYRFSEAS GAIEVPADKV HELRLRLAQQ
110 120 130 140 150
GLPKGGAVGF ELLDQEKFGI SQFSEQVNYQ RALEGELSRT IETIGPVKGA
160 170 180 190 200
RVHLAMPKPS LFVREQKSPS ASVTVNLLPG RALDEGQISA IVHLVSSAVA
210 220 230 240 250
GLPPGNVTLV DQGGHLLTQS NTSGRDLNDA QLKYASDVEG RIQRRIEAIL
260 270 280 290 300
SPIVGNGNIH AQVTAQLDFA SKEQTEEQYR PNGDESHAAL RSRQLNESEQ
310 320 330 340 350
SGSGYPGGVP GALSNQPAPA NNAPISTPPA NQNNRQQQAS TTSNSGPRST
360 370 380 390 400
QRNETSNYEV DRTIRHTKMN VGDVQRLSVA VVVNYKTLPD GKPLPLSNEQ
410 420 430 440 450
MKQIEDLTRE AMGFSEKRGD SLNVVNSPFN SSDESGGELP FWQQQAFIDQ
460 470 480 490 500
LLAAGRWLLV LLVAWLLWRK AVRPQLTRRA EAMKAVQQQA QAREEVEDAV
510 520 530 540 550
EVRLSKDEQL QQRRANQRLG AEVMSQRIRE MSDNDPRVVA LVIRQWINND
HE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 260 | H → R in BAA14029 (Ref. 1) Curated | 1 | |
Sequence conflicti | 266 | Q → H in BAA14029 (Ref. 1) Curated | 1 | |
Sequence conflicti | 541 | L → V (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D89826 Genomic DNA Translation: BAA14029.1 U00096 Genomic DNA Translation: AAC75005.1 AP009048 Genomic DNA Translation: BAA15763.1 M84992 Genomic DNA No translation available. L13243 Genomic DNA No translation available. |
PIRi | G64957 |
RefSeqi | NP_416448.1, NC_000913.3 WP_000994427.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75005; AAC75005; b1938 BAA15763; BAA15763; BAA15763 |
GeneIDi | 946448 |
KEGGi | ecj:JW1922 eco:b1938 |
PATRICi | fig|1411691.4.peg.313 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D89826 Genomic DNA Translation: BAA14029.1 U00096 Genomic DNA Translation: AAC75005.1 AP009048 Genomic DNA Translation: BAA15763.1 M84992 Genomic DNA No translation available. L13243 Genomic DNA No translation available. |
PIRi | G64957 |
RefSeqi | NP_416448.1, NC_000913.3 WP_000994427.1, NZ_LN832404.1 |
3D structure databases
SMRi | P25798 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260383, 20 interactors 850802, 1 interactor |
DIPi | DIP-401N |
IntActi | P25798, 4 interactors |
STRINGi | 511145.b1938 |
Proteomic databases
PaxDbi | P25798 |
PRIDEi | P25798 |
Genome annotation databases
EnsemblBacteriai | AAC75005; AAC75005; b1938 BAA15763; BAA15763; BAA15763 |
GeneIDi | 946448 |
KEGGi | ecj:JW1922 eco:b1938 |
PATRICi | fig|1411691.4.peg.313 |
Organism-specific databases
EchoBASEi | EB1323 |
Phylogenomic databases
eggNOGi | COG1766, Bacteria |
HOGENOMi | CLU_028108_1_0_6 |
InParanoidi | P25798 |
PhylomeDBi | P25798 |
Enzyme and pathway databases
BioCyci | EcoCyc:FLIF-FLAGELLAR-MS-RING |
Miscellaneous databases
PROi | PR:P25798 |
Family and domain databases
InterProi | View protein in InterPro IPR013556, Flag_M-ring_C IPR000067, FlgMring_FliF IPR006182, FliF_N_dom IPR043427, YscJ/FliF |
PANTHERi | PTHR30046, PTHR30046, 1 hit PTHR30046:SF0, PTHR30046:SF0, 1 hit |
Pfami | View protein in Pfam PF01514, YscJ_FliF, 1 hit PF08345, YscJ_FliF_C, 1 hit |
PIRSFi | PIRSF004862, FliF, 1 hit |
PRINTSi | PR01009, FLGMRINGFLIF |
TIGRFAMsi | TIGR00206, fliF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FLIF_ECOLI | |
Accessioni | P25798Primary (citable) accession number: P25798 Secondary accession number(s): P76324, P76914 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families