UniProtKB - P25772 (LIGB_ECOLI)
Protein
DNA ligase B
Gene
ligB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction.1 Publication
Miscellaneous
Considerably less active in nick joining than LigA.
Catalytic activityi
- NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide. EC:6.5.1.2
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 124 | N6-AMP-lysine intermediateCurated | 1 |
GO - Molecular functioni
- DNA ligase (NAD+) activity Source: EcoCyc
GO - Biological processi
- base-excision repair, DNA ligation Source: GO_Central
- DNA ligation Source: EcoCyc
- DNA ligation involved in DNA repair Source: EcoCyc
- DNA replication Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | DNA damage, DNA repair, DNA replication |
Ligand | Magnesium, Manganese, NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11334-MONOMER MetaCyc:EG11334-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA ligase B (EC:6.5.1.2)Alternative name(s): Polydeoxyribonucleotide synthase [NAD(+)] B |
Gene namesi | Name:ligB Synonyms:yicF Ordered Locus Names:b3647, JW3622 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 124 | K → A: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161776 | 1 – 560 | DNA ligase BAdd BLAST | 560 |
Proteomic databases
PaxDbi | P25772 |
PRIDEi | P25772 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262567, 167 interactors |
IntActi | P25772, 3 interactors |
STRINGi | 511145.b3647 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0272, Bacteria |
HOGENOMi | CLU_489786_0_0_6 |
PhylomeDBi | P25772 |
Family and domain databases
HAMAPi | MF_01587, DNA_ligase_B, 1 hit |
InterProi | View protein in InterPro IPR018239, DNA_ligase_AS IPR020923, DNA_ligase_B IPR033136, DNA_ligase_CS IPR013839, DNAligase_adenylation IPR013840, DNAligase_N IPR012340, NA-bd_OB-fold IPR004150, NAD_DNA_ligase_OB IPR010994, RuvA_2-like |
Pfami | View protein in Pfam PF01653, DNA_ligase_aden, 1 hit PF03120, DNA_ligase_OB, 1 hit |
SMARTi | View protein in SMART SM00532, LIGANc, 1 hit |
SUPFAMi | SSF47781, SSF47781, 1 hit SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS01055, DNA_LIGASE_N1, 1 hit PS01056, DNA_LIGASE_N2, 1 hit |
i Sequence
Sequence statusi: Complete.
P25772-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKVWMAILIG ILCWQSSVWA VCPAWSPARA QEEISRLQQQ IKQWDDDYWK
60 70 80 90 100
EGKSEVEDGV YDQLSARLTQ WQRCFGSEPR DVMMPPLNGA VMHPVAHTGV
110 120 130 140 150
RKMVDKNALS LWMRERSDLW VQPKVDGVAV TLVYRDGKLN KAISRGNGLK
160 170 180 190 200
GEDWTQKVSL ISAVPQTVSG PLANSTLQGE IFLQREGHIQ QQMGGINARA
210 220 230 240 250
KVAGLMMRQD DSDTLNSLGV FVWAWPDGPQ LMSDRLKELA TAGFTLTQTY
260 270 280 290 300
TRAVKNADEV ARVRNEWWKA ELPFVTDGVV VRAAKEPESR HWLPGQAEWL
310 320 330 340 350
VAWKYQPVAQ VAEVKAIQFA VGKSGKISVV ASLAPVMLDD KKVQRVNIGS
360 370 380 390 400
VRRWQEWDIA PGDQILVSLA GQGIPRIDDV VWRGAERTKP TPPENRFNSL
410 420 430 440 450
TCYFASDVCQ EQFISRLVWL GAKQVLGLDG IGEAGWRALH QTHRFEHIFS
460 470 480 490 500
WLLLTPEQLQ NTPGIAKSKS AQLWHQFNLA RKQPFTRWVM AMGIPLTRAA
510 520 530 540 550
LNASDERSWS QLLFSTEQFW QQLPGTGSGR ARQVIEWKEN AQIKKLGSWL
560
AAQQITGFEP
Sequence cautioni
The sequence AAA62000 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAB88710 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62000.1 Different initiation. U00096 Genomic DNA Translation: AAC76671.2 AP009048 Genomic DNA Translation: BAE77646.1 M84400 Genomic DNA Translation: AAB88710.1 Different initiation. |
PIRi | A65166 |
RefSeqi | NP_418104.2, NC_000913.3 WP_000870036.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76671; AAC76671; b3647 BAE77646; BAE77646; BAE77646 |
GeneIDi | 948164 |
KEGGi | ecj:JW3622 eco:b3647 |
PATRICi | fig|511145.12.peg.3767 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62000.1 Different initiation. U00096 Genomic DNA Translation: AAC76671.2 AP009048 Genomic DNA Translation: BAE77646.1 M84400 Genomic DNA Translation: AAB88710.1 Different initiation. |
PIRi | A65166 |
RefSeqi | NP_418104.2, NC_000913.3 WP_000870036.1, NZ_LN832404.1 |
3D structure databases
SMRi | P25772 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262567, 167 interactors |
IntActi | P25772, 3 interactors |
STRINGi | 511145.b3647 |
Proteomic databases
PaxDbi | P25772 |
PRIDEi | P25772 |
Protocols and materials databases
DNASUi | 948164 |
Genome annotation databases
EnsemblBacteriai | AAC76671; AAC76671; b3647 BAE77646; BAE77646; BAE77646 |
GeneIDi | 948164 |
KEGGi | ecj:JW3622 eco:b3647 |
PATRICi | fig|511145.12.peg.3767 |
Organism-specific databases
EchoBASEi | EB1310 |
Phylogenomic databases
eggNOGi | COG0272, Bacteria |
HOGENOMi | CLU_489786_0_0_6 |
PhylomeDBi | P25772 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11334-MONOMER MetaCyc:EG11334-MONOMER |
Miscellaneous databases
PROi | PR:P25772 |
Family and domain databases
HAMAPi | MF_01587, DNA_ligase_B, 1 hit |
InterProi | View protein in InterPro IPR018239, DNA_ligase_AS IPR020923, DNA_ligase_B IPR033136, DNA_ligase_CS IPR013839, DNAligase_adenylation IPR013840, DNAligase_N IPR012340, NA-bd_OB-fold IPR004150, NAD_DNA_ligase_OB IPR010994, RuvA_2-like |
Pfami | View protein in Pfam PF01653, DNA_ligase_aden, 1 hit PF03120, DNA_ligase_OB, 1 hit |
SMARTi | View protein in SMART SM00532, LIGANc, 1 hit |
SUPFAMi | SSF47781, SSF47781, 1 hit SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS01055, DNA_LIGASE_N1, 1 hit PS01056, DNA_LIGASE_N2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LIGB_ECOLI | |
Accessioni | P25772Primary (citable) accession number: P25772 Secondary accession number(s): P78120, Q2M7W0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families