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Protein

Chitinase 12

Gene

Cht12

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani).1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  • beta-N-acetylglucosaminidase activity Source: Gramene
  • chitinase activity Source: UniProtKB
  • chitin binding Source: Gramene
  • endochitinase activity Source: Gramene

GO - Biological processi

  • amino sugar metabolic process Source: Gramene
  • cell wall macromolecule catabolic process Source: InterPro
  • chitin catabolic process Source: UniProtKB-KW
  • defense response to fungus Source: UniProtKB
  • polysaccharide catabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
GH19 Glycoside Hydrolase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 12 (EC:3.2.1.14)
Alternative name(s):
Basic endochitinase 2
Pathogenesis related (PR)-3 chitinase 12
Gene namesi
Name:Cht12
Synonyms:RCH10
Ordered Locus Names:Os03g0418000, LOC_Os03g30470
ORF Names:OSJNBb0028K20.4, OSJNBb0056B16.17
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000530622 – 326Chitinase 12Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 39PROSITE-ProRule annotation
Disulfide bondi33 ↔ 45PROSITE-ProRule annotation
Disulfide bondi36 ↔ 65PROSITE-ProRule annotation
Disulfide bondi38 ↔ 52PROSITE-ProRule annotation
Disulfide bondi56 ↔ 60PROSITE-ProRule annotation
Disulfide bondi102 ↔ 165PROSITE-ProRule annotation
Disulfide bondi179 ↔ 187PROSITE-ProRule annotation
Disulfide bondi286 ↔ 318PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP25765

Expressioni

Tissue specificityi

Expressed in meristems and at lower levels in roots and sheaths.1 Publication

Gene expression databases

ExpressionAtlasiP25765 differential

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g30470.1

Structurei

3D structure databases

ProteinModelPortaliP25765
SMRiP25765
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 62Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742 Eukaryota
COG3979 LUCA
HOGENOMiHOG000231411
InParanoidiP25765
OMAiCQQSAQW
OrthoDBiEOG09360IMR

Family and domain databases

CDDicd00325 chitinase_glyco_hydro_19, 1 hit
Gene3Di3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR036861 Endochitinase-like_sf
IPR016283 Glyco_hydro_19
IPR000726 Glyco_hydro_19_cat
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00182 Glyco_hydro_19, 1 hit
PIRSFiPIRSF001060 Endochitinase, 1 hit
PRINTSiPR00451 CHITINBINDNG
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS00773 CHITINASE_19_1, 1 hit
PS00774 CHITINASE_19_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALAVVAMV ATAFLAAAVH AEQCGSQAGG AVCPNCLCCS QFGWCGSTSD
60 70 80 90 100
YCGAGCQSQC SAAGCGGGGP TPPSGSGGSG VASIVSRSLF DQMLLHRNDA
110 120 130 140 150
ACPASNFYTY DAFVAAASAF PGFAAAGGDA DTNKREVAAF LAQTSHETTG
160 170 180 190 200
GWATAPDGPY AWGYCFKEEN GGAAGPDYCQ QSAQWPCAAG KKYYGRGPIQ
210 220 230 240 250
LSYNFNYGPA GQAIGADLLG DPDLVASDAT VSFDTAFWFW MTPQSPKPSC
260 270 280 290 300
HAVATGQWTP SADDQAAGRV PGYGVITNII NGGLECGHGE DDRVADRIGF
310 320
YKRYCDILGV SYDANLDCYS QRPFGS
Length:326
Mass (Da):33,636
Last modified:September 5, 2006 - v2
Checksum:i41E748706FAFEDC1
GO

Sequence cautioni

The sequence described in PubMed:2034221 differs from that shown. Sequencing errors.Curated
The sequence described in PubMed:2034221 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137992 Genomic DNA Translation: AAP44624.1
AC145386 Genomic DNA Translation: AAR01697.1
DP000009 Genomic DNA Translation: ABF96639.1
AP008209 Genomic DNA Translation: BAF12287.2
AP014959 Genomic DNA Translation: BAS84717.1
PIRiS15997
RefSeqiXP_015629397.1, XM_015773911.1

Genome annotation databases

EnsemblPlantsiOs03t0418000-00; Os03t0418000-00; Os03g0418000
GeneIDi4333121
GrameneiOs03t0418000-00; Os03t0418000-00; Os03g0418000
KEGGiosa:4333121

Similar proteinsi

Entry informationi

Entry nameiCHI12_ORYSJ
AccessioniPrimary (citable) accession number: P25765
Secondary accession number(s): A0A0P0VYS9, Q0DR51, Q7Y1K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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