UniProtKB - P25746 (HFLD_ECOLI)
Protein
High frequency lysogenization protein HflD
Gene
hflD
Organism
Escherichia coli (strain K12)
Status
Functioni
Negative regulator of phage lambda lysogenization. Contributes to the degradation of the phage regulatory protein CII. Acts probably by holding CII on the membrane surface, away from the target promoters, but close to the FtsH protease.1 Publication
GO - Biological processi
- response to heat Source: EcoliWiki
Enzyme and pathway databases
BioCyci | EcoCyc:EG11345-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: High frequency lysogenization protein HflD |
Gene namesi | Name:hflD Synonyms:ycfC Ordered Locus Names:b1132, JW5165 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene show an increased lysogenization frequency.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000071576 | 1 – 213 | High frequency lysogenization protein HflDAdd BLAST | 213 |
Proteomic databases
jPOSTi | P25746 |
PaxDbi | P25746 |
PRIDEi | P25746 |
Interactioni
Subunit structurei
Interacts with CII protein from phage lambda.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263130, 20 interactors |
STRINGi | 511145.b1132 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P25746 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P25746 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 79 – 126 | Sequence analysisAdd BLAST | 48 |
Sequence similaritiesi
Belongs to the HflD family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | COG2915, Bacteria |
HOGENOMi | CLU_098920_0_0_6 |
InParanoidi | P25746 |
Family and domain databases
Gene3Di | 1.10.3890.10, 1 hit |
HAMAPi | MF_00695, HflD_protein, 1 hit |
InterProi | View protein in InterPro IPR007451, HflD IPR035932, HflD-like_sf |
PANTHERi | PTHR38100, PTHR38100, 1 hit |
Pfami | View protein in Pfam PF04356, DUF489, 1 hit |
SUPFAMi | SSF101322, SSF101322, 1 hit |
i Sequence
Sequence statusi: Complete.
P25746-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKNYYDITL ALAGICQSAR LVQQLAHQGH CDADALHVSL NSIIDMNPSS
60 70 80 90 100
TLAVFGGSEA NLRVGLETLL GVLNASSRQG LNAELTRYTL SLMVLERKLS
110 120 130 140 150
SAKGALDTLG NRINGLQRQL EHFDLQSETL MSAMAAIYVD VISPLGPRIQ
160 170 180 190 200
VTGSPAVLQS PQVQAKVRAT LLAGIRAAVL WHQVGGGRLQ LMFSRNRLTT
210
QAKQILAHLT PEL
Sequence cautioni
The sequence M74924 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59307 Genomic DNA Translation: CAA41995.1 M74924 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC74216.1 AP009048 Genomic DNA Translation: BAA35954.1 |
PIRi | S19211 |
RefSeqi | NP_415650.1, NC_000913.3 WP_001297479.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74216; AAC74216; b1132 BAA35954; BAA35954; BAA35954 |
GeneIDi | 58347357 945693 |
KEGGi | ecj:JW5165 eco:b1132 |
PATRICi | fig|1411691.4.peg.1134 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59307 Genomic DNA Translation: CAA41995.1 M74924 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC74216.1 AP009048 Genomic DNA Translation: BAA35954.1 |
PIRi | S19211 |
RefSeqi | NP_415650.1, NC_000913.3 WP_001297479.1, NZ_STEB01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QZ4 | X-ray | 2.00 | A | 2-213 | [»] | |
1SDI | X-ray | 1.65 | A | 2-213 | [»] | |
SMRi | P25746 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263130, 20 interactors |
STRINGi | 511145.b1132 |
Proteomic databases
jPOSTi | P25746 |
PaxDbi | P25746 |
PRIDEi | P25746 |
Genome annotation databases
EnsemblBacteriai | AAC74216; AAC74216; b1132 BAA35954; BAA35954; BAA35954 |
GeneIDi | 58347357 945693 |
KEGGi | ecj:JW5165 eco:b1132 |
PATRICi | fig|1411691.4.peg.1134 |
Organism-specific databases
EchoBASEi | EB1321 |
Phylogenomic databases
eggNOGi | COG2915, Bacteria |
HOGENOMi | CLU_098920_0_0_6 |
InParanoidi | P25746 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11345-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P25746 |
PROi | PR:P25746 |
Family and domain databases
Gene3Di | 1.10.3890.10, 1 hit |
HAMAPi | MF_00695, HflD_protein, 1 hit |
InterProi | View protein in InterPro IPR007451, HflD IPR035932, HflD-like_sf |
PANTHERi | PTHR38100, PTHR38100, 1 hit |
Pfami | View protein in Pfam PF04356, DUF489, 1 hit |
SUPFAMi | SSF101322, SSF101322, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HFLD_ECOLI | |
Accessioni | P25746Primary (citable) accession number: P25746 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families