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UniProtKB - P25737 (LYSP_ECOLI)
Protein
Lysine-specific permease
Gene
lysP
Organism
Escherichia coli (strain K12)
Status
Functioni
Permease that is involved in the transport across the cytoplasmic membrane of lysine.
GO - Molecular functioni
- amino acid transmembrane transporter activity Source: GO_Central
- identical protein binding Source: EcoCyc
- L-lysine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- amino acid transmembrane transport Source: GO_Central
- L-lysine import across plasma membrane Source: EcoCyc
- regulation of DNA-binding transcription factor activity Source: EcoCyc
- response to amino acid Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:LYSP-MONOMER |
Protein family/group databases
TCDBi | 2.A.3.1.2, the amino acid-polyamine-organocation (apc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Lysine-specific permease |
Gene namesi | Name:lysP Synonyms:cadR Ordered Locus Names:b2156, JW2143 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 21 | CytoplasmicCuratedAdd BLAST | 20 | |
Transmembranei | 22 – 42 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 43 – 55 | PeriplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 56 – 76 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 77 – 104 | CytoplasmicCuratedAdd BLAST | 28 | |
Transmembranei | 105 – 125 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 126 – 130 | PeriplasmicCurated | 5 | |
Transmembranei | 131 – 149 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 150 – 160 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 161 – 181 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 182 – 199 | PeriplasmicCuratedAdd BLAST | 18 | |
Transmembranei | 200 – 218 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 219 – 244 | CytoplasmicCuratedAdd BLAST | 26 | |
Transmembranei | 245 – 264 | HelicalCuratedAdd BLAST | 20 | |
Topological domaini | 265 – 283 | PeriplasmicCuratedAdd BLAST | 19 | |
Transmembranei | 284 – 304 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 305 – 347 | CytoplasmicCuratedAdd BLAST | 43 | |
Transmembranei | 348 – 365 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 366 – 371 | PeriplasmicCurated | 6 | |
Transmembranei | 372 – 392 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 393 – 415 | CytoplasmicCuratedAdd BLAST | 23 | |
Transmembranei | 416 – 433 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 434 – 449 | PeriplasmicCuratedAdd BLAST | 16 | |
Transmembranei | 450 – 470 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 471 – 489 | CytoplasmicCuratedAdd BLAST | 19 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000054204 | 2 – 489 | Lysine-specific permeaseAdd BLAST | 488 |
Proteomic databases
PaxDbi | P25737 |
PRIDEi | P25737 |
Expressioni
Inductioni
High, in anaerobic media of low pH, containing lysine.
Interactioni
Binary interactionsi
P25737
With | #Exp. | IntAct |
---|---|---|
cadC [P23890] | 2 | EBI-6401655,EBI-6401662 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260458, 13 interactors |
IntActi | P25737, 1 interactor |
STRINGi | 511145.b2156 |
Family & Domainsi
Sequence similaritiesi
Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0833, Bacteria |
HOGENOMi | CLU_007946_9_2_6 |
InParanoidi | P25737 |
PhylomeDBi | P25737 |
Family and domain databases
InterProi | View protein in InterPro IPR004841, AA-permease/SLC12A_dom IPR004840, Amoino_acid_permease_CS |
Pfami | View protein in Pfam PF00324, AA_permease, 1 hit |
PROSITEi | View protein in PROSITE PS00218, AMINO_ACID_PERMEASE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P25737-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSETKTTEA PGLRRELKAR HLTMIAIGGS IGTGLFVASG ATISQAGPGG
60 70 80 90 100
ALLSYMLIGL MVYFLMTSLG ELAAYMPVSG SFATYGQNYV EEGFGFALGW
110 120 130 140 150
NYWYNWAVTI AVDLVAAQLV MSWWFPDTPG WIWSALFLGV IFLLNYISVR
160 170 180 190 200
GFGEAEYWFS LIKVTTVIVF IIVGVLMIIG IFKGAQPAGW SNWTIGEAPF
210 220 230 240 250
AGGFAAMIGV AMIVGFSFQG TELIGIAAGE SEDPAKNIPR AVRQVFWRIL
260 270 280 290 300
LFYVFAILII SLIIPYTDPS LLRNDVKDIS VSPFTLVFQH AGLLSAAAVM
310 320 330 340 350
NAVILTAVLS AGNSGMYAST RMLYTLACDG KAPRIFAKLS RGGVPRNALY
360 370 380 390 400
ATTVIAGLCF LTSMFGNQTV YLWLLNTSGM TGFIAWLGIA ISHYRFRRGY
410 420 430 440 450
VLQGHDINDL PYRSGFFPLG PIFAFILCLI ITLGQNYEAF LKDTIDWGGV
460 470 480
AATYIGIPLF LIIWFGYKLI KGTHFVRYSE MKFPQNDKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 122 | S → N in AAA60532 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M89774 Genomic DNA Translation: AAA17053.1 U00007 Genomic DNA Translation: AAA60532.1 U00096 Genomic DNA Translation: AAC75217.1 AP009048 Genomic DNA Translation: BAE76633.1 |
PIRi | C64984 |
RefSeqi | NP_416661.1, NC_000913.3 WP_000253273.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75217; AAC75217; b2156 BAE76633; BAE76633; BAE76633 |
GeneIDi | 66673948 946667 |
KEGGi | ecj:JW2143 eco:b2156 |
PATRICi | fig|1411691.4.peg.84 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M89774 Genomic DNA Translation: AAA17053.1 U00007 Genomic DNA Translation: AAA60532.1 U00096 Genomic DNA Translation: AAC75217.1 AP009048 Genomic DNA Translation: BAE76633.1 |
PIRi | C64984 |
RefSeqi | NP_416661.1, NC_000913.3 WP_000253273.1, NZ_STEB01000002.1 |
3D structure databases
SMRi | P25737 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260458, 13 interactors |
IntActi | P25737, 1 interactor |
STRINGi | 511145.b2156 |
Protein family/group databases
TCDBi | 2.A.3.1.2, the amino acid-polyamine-organocation (apc) family |
Proteomic databases
PaxDbi | P25737 |
PRIDEi | P25737 |
Genome annotation databases
EnsemblBacteriai | AAC75217; AAC75217; b2156 BAE76633; BAE76633; BAE76633 |
GeneIDi | 66673948 946667 |
KEGGi | ecj:JW2143 eco:b2156 |
PATRICi | fig|1411691.4.peg.84 |
Organism-specific databases
EchoBASEi | EB1313 |
Phylogenomic databases
eggNOGi | COG0833, Bacteria |
HOGENOMi | CLU_007946_9_2_6 |
InParanoidi | P25737 |
PhylomeDBi | P25737 |
Enzyme and pathway databases
BioCyci | EcoCyc:LYSP-MONOMER |
Miscellaneous databases
PROi | PR:P25737 |
Family and domain databases
InterProi | View protein in InterPro IPR004841, AA-permease/SLC12A_dom IPR004840, Amoino_acid_permease_CS |
Pfami | View protein in Pfam PF00324, AA_permease, 1 hit |
PROSITEi | View protein in PROSITE PS00218, AMINO_ACID_PERMEASE_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LYSP_ECOLI | |
Accessioni | P25737Primary (citable) accession number: P25737 Secondary accession number(s): Q2MAS3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 164 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families