UniProtKB - P25694 (CDC48_YEAST)
Cell division control protein 48
CDC48
Functioni
Miscellaneous
Catalytic activityi
- EC:3.6.4.61 Publication
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 358 | ATP 1By similarity | 1 | |
Binding sitei | 394 | ATP 1By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 257 – 263 | ATP 1By similarity | 7 | |
Nucleotide bindingi | 531 – 536 | ATP 2By similarity | 6 |
GO - Molecular functioni
- ATPase activity Source: SGD
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- polyubiquitin modification-dependent protein binding Source: GO_Central
- protein phosphatase regulator activity Source: SGD
- ubiquitin binding Source: SGD
GO - Biological processi
- ATP metabolic process Source: ParkinsonsUK-UCL
- autophagosome maturation Source: GO_Central
- cellular protein complex disassembly Source: SGD
- cytoplasm protein quality control by the ubiquitin-proteasome system Source: SGD
- DNA replication termination Source: SGD
- endoplasmic reticulum membrane fusion Source: SGD
- ER-associated misfolded protein catabolic process Source: SGD
- macroautophagy Source: SGD
- mitochondria-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
- mitotic spindle disassembly Source: SGD
- negative regulation of telomerase activity Source: SGD
- nonfunctional rRNA decay Source: SGD
- nuclear protein quality control by the ubiquitin-proteasome system Source: SGD
- piecemeal microautophagy of the nucleus Source: SGD
- positive regulation of histone H2B ubiquitination Source: SGD
- positive regulation of mitochondrial fusion Source: SGD
- positive regulation of protein localization to nucleus Source: SGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
- protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
- retrograde protein transport, ER to cytosol Source: SGD
- ribophagy Source: SGD
- ribosome-associated ubiquitin-dependent protein catabolic process Source: SGD
- SCF complex disassembly in response to cadmium stress Source: SGD
- sister chromatid biorientation Source: SGD
- stress-induced homeostatically regulated protein degradation pathway Source: SGD
- ubiquitin-dependent ERAD pathway Source: SGD
Keywordsi
Molecular function | Chaperone, Hydrolase |
Biological process | Cell cycle, Protein transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-3371511, HSF1 activation R-SCE-5689896, Ovarian tumor domain proteases R-SCE-6798695, Neutrophil degranulation R-SCE-8876725, Protein methylation |
Protein family/group databases
TCDBi | 3.A.16.1.2, the endoplasmic reticular retrotranslocon (er-rt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division control protein 481 Publication (EC:3.6.4.61 Publication)Alternative name(s): Cell division cycle protein 481 Publication Transitional endoplasmic reticulum ATPase homologCurated |
Gene namesi | Name:CDC481 Publication Ordered Locus Names:YDL126CImported |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YDL126C |
SGDi | S000002284, CDC48 |
Subcellular locationi
Endoplasmic reticulum
- Microsome 1 Publication
- Endoplasmic reticulum 1 Publication
Other locations
- Cytoplasm 1 Publication
Note: Bound loosely to components of the microsomal fraction.1 Publication
Cytosol
- cytosol Source: SGD
Endoplasmic reticulum
- Doa10p ubiquitin ligase complex Source: SGD
- endoplasmic reticulum membrane Source: SGD
- Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
- VCP-NPL4-UFD1 AAA ATPase complex Source: SGD
Mitochondrion
- Cdc48p-Npl4p-Vms1p AAA ATPase complex Source: SGD
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: GO_Central
- mating projection tip Source: SGD
- replisome Source: SGD
- RQC complex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MicrosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 261 | K → A: Moderate reduction in growth rate. 1 Publication | 1 | |
Mutagenesisi | 261 | K → T: Probable loss of ATP binding. Complete loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 315 | E → A: Moderate reduction in growth rate. 1 Publication | 1 | |
Mutagenesisi | 315 | E → D: Severe loss of catalytic activity without affecting cooperativity between the 2 ATP-binding regions. Slight reduction in growth rate. 1 Publication | 1 | |
Mutagenesisi | 315 | E → N: Severe reduction in growth rate. 1 Publication | 1 | |
Mutagenesisi | 315 | E → Q: Severe loss of catalytic activity and cooperativity between the 2 ATP-binding regions. Lethal. Restores cell growth; when associated with A-358; A-369; S-471; A-471 or H-475. 1 Publication | 1 | |
Mutagenesisi | 358 | N → A: Slight reduction in growth rate. Restores cell growth; when associated with Q-315. 1 Publication | 1 | |
Mutagenesisi | 369 | R → A: No effect on growth rate. Restores cell growth; when associated with Q-315. 1 Publication | 1 | |
Mutagenesisi | 471 | P → A or S: Restores cell growth; when associated with Q-315. 1 Publication | 1 | |
Mutagenesisi | 475 | R → H: Restores cell growth; when associated with Q-315. 1 Publication | 1 | |
Mutagenesisi | 534 | K → A or T: Severe loss of catalytic activity. Lethal. 1 Publication | 1 | |
Mutagenesisi | 588 | E → D: Moderate reduction in growth rate. 1 Publication | 1 | |
Mutagenesisi | 588 | E → Q: Lethal. 1 Publication | 1 | |
Mutagenesisi | 645 | R → A: Lethal. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084587 | 1 – 835 | Cell division control protein 48Add BLAST | 835 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 305 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 322 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 346 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 472 | PhosphoserineCombined sources | 1 | |
Modified residuei | 519 | PhosphoserineCombined sources | 1 | |
Cross-linki | 522 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 539 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 594 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 673 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 735 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 770 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P25694 |
PaxDbi | P25694 |
PRIDEi | P25694 |
PTM databases
CarbonylDBi | P25694 |
iPTMneti | P25694 |
Interactioni
Subunit structurei
Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1 (PubMed:16873066). The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1 (PubMed:16873066).
Interacts with HRD1, HRD3, YOS9, UBX2, DER1, USA1 and UFD1 (PubMed:16873066).
Forms a complex composed of CDC48, NPL4, UFD1, UFD2 and SHP1 (PubMed:16427015).
Forms a complex composed of CDC48, NPL4, UFD1, DOA1, SHP1 and deubiquitinase OTU1; within the complex interacts with DOA1/UFD3 and OTU1 to prevent multiubiquitination of substrates (PubMed:16427015).
Interacts with UFD2, to add further ubiquitin moieties; the interaction with UFD2 is prevented by DOA1/UFD3 (PubMed:16427015).
Forms a complex composed of CDC48, DOA1, deubiquitinase UBP3 and probably BRE5; within the complex interacts with DOA1 and UBP3 (PubMed:20508643).
Interacts (via C-terminus) with DOA1 (via PUL domain); the interaction is direct (PubMed:16428438, PubMed:19805280, PubMed:27044889).
Interacts with NPL4 (PubMed:11733065, PubMed:11598205, PubMed:31249134).
Interacts with SHP1/UBX1, UBX2, UBX3, UBX4, UBX5, UBX6 and UBX7 (PubMed:15258615, PubMed:31249134).
Interacts with VMS1; the interaction recruits CDC48 to the mitochondria in response to mitochondrial stress (PubMed:21070972, PubMed:21148305).
Component of the ribosome quality control complex (RQC), composed of the E3 ubiquitin ligase RKR1/LTN1, RQC1 and RQC2, as well as CDC48 and its ubiquitin-binding cofactors (PubMed:23178123, PubMed:23479637). RQC forms a stable complex with 60S ribosomal subunits (PubMed:23178123, PubMed:23479637).
Interacts with ASE1 and CDC5; the interaction is likely to result in their degradation (PubMed:14636562).
Component of the DSCc E3 ligase complexes composed of at least TUL1, DSC2, DSC3, UBX3, CDC48 as well as VLD1 for the vacuole-localized complex or GLD1 for the Golgi/endosome-localized complex (PubMed:29355480).
16 PublicationsBinary interactionsi
Hide detailsP25694
GO - Molecular functioni
- identical protein binding Source: IntAct
- polyubiquitin modification-dependent protein binding Source: GO_Central
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31937, 891 interactors |
ComplexPortali | CPX-1323, CDC48-RAD23-UFD2 complex CPX-2946, CDC48-NPL4-UFD1 AAA ATPase complex CPX-3069, Cdc48-Npl4-Vms1 AAA ATPase complex CPX-3265, RQC complex |
DIPi | DIP-2704N |
IntActi | P25694, 166 interactors |
MINTi | P25694 |
STRINGi | 4932.YDL126C |
Miscellaneous databases
RNActi | P25694, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0730, Eukaryota |
GeneTreei | ENSGT00940000165417 |
HOGENOMi | CLU_000688_12_2_1 |
InParanoidi | P25694 |
OMAi | PIDDTTE |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR005938, AAA_ATPase_CDC48 IPR041569, AAA_lid_3 IPR009010, Asp_de-COase-like_dom_sf IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR004201, Cdc48_dom2 IPR029067, CDC48_domain_2-like_sf IPR003338, CDC4_N-term_subdom IPR027417, P-loop_NTPase IPR015415, Vps4_C |
Pfami | View protein in Pfam PF00004, AAA, 2 hits PF17862, AAA_lid_3, 2 hits PF02933, CDC48_2, 1 hit PF02359, CDC48_N, 1 hit PF09336, Vps4_C, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 2 hits SM01072, CDC48_2, 1 hit SM01073, CDC48_N, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF52540, SSF52540, 2 hits SSF54585, SSF54585, 1 hit |
TIGRFAMsi | TIGR01243, CDC48, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGEEHKPLLD ASGVDPREED KTATAILRRK KKDNMLLVDD AINDDNSVIA
60 70 80 90 100
INSNTMDKLE LFRGDTVLVK GKKRKDTVLI VLIDDELEDG ACRINRVVRN
110 120 130 140 150
NLRIRLGDLV TIHPCPDIKY ATRISVLPIA DTIEGITGNL FDVFLKPYFV
160 170 180 190 200
EAYRPVRKGD HFVVRGGMRQ VEFKVVDVEP EEYAVVAQDT IIHWEGEPIN
210 220 230 240 250
REDEENNMNE VGYDDIGGCR KQMAQIREMV ELPLRHPQLF KAIGIKPPRG
260 270 280 290 300
VLMYGPPGTG KTLMARAVAN ETGAFFFLIN GPEVMSKMAG ESESNLRKAF
310 320 330 340 350
EEAEKNAPAI IFIDEIDSIA PKRDKTNGEV ERRVVSQLLT LMDGMKARSN
360 370 380 390 400
VVVIAATNRP NSIDPALRRF GRFDREVDIG IPDATGRLEV LRIHTKNMKL
410 420 430 440 450
ADDVDLEALA AETHGYVGAD IASLCSEAAM QQIREKMDLI DLDEDEIDAE
460 470 480 490 500
VLDSLGVTMD NFRFALGNSN PSALRETVVE SVNVTWDDVG GLDEIKEELK
510 520 530 540 550
ETVEYPVLHP DQYTKFGLSP SKGVLFYGPP GTGKTLLAKA VATEVSANFI
560 570 580 590 600
SVKGPELLSM WYGESESNIR DIFDKARAAA PTVVFLDELD SIAKARGGSL
610 620 630 640 650
GDAGGASDRV VNQLLTEMDG MNAKKNVFVI GATNRPDQID PAILRPGRLD
660 670 680 690 700
QLIYVPLPDE NARLSILNAQ LRKTPLEPGL ELTAIAKATQ GFSGADLLYI
710 720 730 740 750
VQRAAKYAIK DSIEAHRQHE AEKEVKVEGE DVEMTDEGAK AEQEPEVDPV
760 770 780 790 800
PYITKEHFAE AMKTAKRSVS DAELRRYEAY SQQMKASRGQ FSNFNFNDAP
810 820 830
LGTTATDNAN SNNSAPSGAG AAFGSNAEED DDLYS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56956 Genomic DNA Translation: CAA40276.1 Z74174 Genomic DNA Translation: CAA98694.1 BK006938 Genomic DNA Translation: DAA11734.1 |
PIRi | S67669 |
RefSeqi | NP_010157.1, NM_001180185.1 |
Genome annotation databases
EnsemblFungii | YDL126C_mRNA; YDL126C; YDL126C |
GeneIDi | 851431 |
KEGGi | sce:YDL126C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56956 Genomic DNA Translation: CAA40276.1 Z74174 Genomic DNA Translation: CAA98694.1 BK006938 Genomic DNA Translation: DAA11734.1 |
PIRi | S67669 |
RefSeqi | NP_010157.1, NM_001180185.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6OA9 | electron microscopy | 3.90 | A/B/C/D/E/F | 1-835 | [»] | |
6OAA | electron microscopy | 4.10 | B/C/D/E | 1-835 | [»] | |
6OAB | electron microscopy | 3.60 | A/B/C/D/E | 1-835 | [»] | |
6OMB | electron microscopy | 3.70 | A/B/C/D/E | 1-835 | [»] | |
6OPC | electron microscopy | 3.70 | A/B/C/D/E/F | 1-835 | [»] | |
SMRi | P25694 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31937, 891 interactors |
ComplexPortali | CPX-1323, CDC48-RAD23-UFD2 complex CPX-2946, CDC48-NPL4-UFD1 AAA ATPase complex CPX-3069, Cdc48-Npl4-Vms1 AAA ATPase complex CPX-3265, RQC complex |
DIPi | DIP-2704N |
IntActi | P25694, 166 interactors |
MINTi | P25694 |
STRINGi | 4932.YDL126C |
Protein family/group databases
TCDBi | 3.A.16.1.2, the endoplasmic reticular retrotranslocon (er-rt) family |
PTM databases
CarbonylDBi | P25694 |
iPTMneti | P25694 |
Proteomic databases
MaxQBi | P25694 |
PaxDbi | P25694 |
PRIDEi | P25694 |
Genome annotation databases
EnsemblFungii | YDL126C_mRNA; YDL126C; YDL126C |
GeneIDi | 851431 |
KEGGi | sce:YDL126C |
Organism-specific databases
EuPathDBi | FungiDB:YDL126C |
SGDi | S000002284, CDC48 |
Phylogenomic databases
eggNOGi | KOG0730, Eukaryota |
GeneTreei | ENSGT00940000165417 |
HOGENOMi | CLU_000688_12_2_1 |
InParanoidi | P25694 |
OMAi | PIDDTTE |
Enzyme and pathway databases
Reactomei | R-SCE-3371511, HSF1 activation R-SCE-5689896, Ovarian tumor domain proteases R-SCE-6798695, Neutrophil degranulation R-SCE-8876725, Protein methylation |
Miscellaneous databases
PROi | PR:P25694 |
RNActi | P25694, protein |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR005938, AAA_ATPase_CDC48 IPR041569, AAA_lid_3 IPR009010, Asp_de-COase-like_dom_sf IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR004201, Cdc48_dom2 IPR029067, CDC48_domain_2-like_sf IPR003338, CDC4_N-term_subdom IPR027417, P-loop_NTPase IPR015415, Vps4_C |
Pfami | View protein in Pfam PF00004, AAA, 2 hits PF17862, AAA_lid_3, 2 hits PF02933, CDC48_2, 1 hit PF02359, CDC48_N, 1 hit PF09336, Vps4_C, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 2 hits SM01072, CDC48_2, 1 hit SM01073, CDC48_N, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF52540, SSF52540, 2 hits SSF54585, SSF54585, 1 hit |
TIGRFAMsi | TIGR01243, CDC48, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDC48_YEAST | |
Accessioni | P25694Primary (citable) accession number: P25694 Secondary accession number(s): D6VRM4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 208 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names