Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cell division control protein 48

Gene

CDC48

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in spindle disassembly, degradation of ubiquitinated proteins and protein export from the endoplasmic reticulum to the cytoplasm. Acts as a chaperone that collects ubiquitinated substrates. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway. Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1. Has an additional role in the turnover of OLE1 where it targets ubiquitinated OLE1 and other proteins to the ERAD (PubMed:11733065, PubMed:11740563, PubMed:11813000, PubMed:11847109, PubMed:21070972, PubMed:21148305). Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. CDC48 may provide the mechanical force that dislodges the polyubiquitinated nascent peptides from the exit channel (PubMed:23178123, PubMed:24261871).8 Publications

Miscellaneous

Present with 78400 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei358ATPBy similarity1
Binding sitei394ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi257 – 263ATPBy similarity7

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • protein phosphatase regulator activity Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

Keywordsi

Biological processCell cycle, Protein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29525-MONOMER
ReactomeiR-SCE-110320 Translesion Synthesis by POLH
R-SCE-3371511 HSF1 activation
R-SCE-5689896 Ovarian tumor domain proteases
R-SCE-6798695 Neutrophil degranulation
R-SCE-8876725 Protein methylation

Protein family/group databases

TCDBi3.A.16.1.2 the endoplasmic reticular retrotranslocon (er-rt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 481 Publication
Alternative name(s):
Cell division cycle protein 481 Publication
Gene namesi
Name:CDC481 Publication
Ordered Locus Names:YDL126CImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL126C
SGDiS000002284 CDC48

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845871 – 835Cell division control protein 48Add BLAST835

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki305Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki346Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei472PhosphoserineCombined sources1
Modified residuei519PhosphoserineCombined sources1
Cross-linki522Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki594Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki673Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei735PhosphothreonineCombined sources1
Modified residuei770PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP25694
PaxDbiP25694
PRIDEiP25694

PTM databases

CarbonylDBiP25694
iPTMnetiP25694

Interactioni

Subunit structurei

Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with HRD1, HRD3, YOS9, UBX2, DER1, USA1 and UFD1. Interacts with OTU1 and DOA1/UFD3, to prevent multiubiquitination of substrates. Interacts with UFD2, to add further ubiquitin moieties; the interaction with UFD2 is prevented by DOA1/UFD3. Interacts with NPL4 and VMS1. Component of the ribosome quality control complex (RQC), composed of the E3 ubiquitin ligase RKR1/LTN1, RQC1 and RQC2, as well as CDC48 and its ubiquitin-binding cofactors. RQC forms a stable complex with 60S ribosomal subunits (PubMed:23178123, PubMed:23479637).9 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31937, 870 interactors
ComplexPortaliCPX-1323 CDC48-RAD23-UFD2 complex
CPX-2946 CDC48-NPL4-UFD1 AAA ATPase complex
CPX-3069 Cdc48-Npl4-Vms1 AAA ATPase complex
CPX-3265 RQC complex
DIPiDIP-2704N
IntActiP25694, 166 interactors
MINTiP25694
STRINGi4932.YDL126C

Structurei

3D structure databases

ProteinModelPortaliP25694
SMRiP25694
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00900000141071
HOGENOMiHOG000223224
InParanoidiP25694
KOiK13525
OMAiPIDDTTE
OrthoDBiEOG092C0QVL

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR005938 AAA_ATPase_CDC48
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
IPR015415 Vps4_C
PfamiView protein in Pfam
PF00004 AAA, 2 hits
PF02933 CDC48_2, 1 hit
PF02359 CDC48_N, 1 hit
PF09336 Vps4_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01072 CDC48_2, 1 hit
SM01073 CDC48_N, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit
TIGRFAMsiTIGR01243 CDC48, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 2 hits

Sequencei

Sequence statusi: Complete.

P25694-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGEEHKPLLD ASGVDPREED KTATAILRRK KKDNMLLVDD AINDDNSVIA
60 70 80 90 100
INSNTMDKLE LFRGDTVLVK GKKRKDTVLI VLIDDELEDG ACRINRVVRN
110 120 130 140 150
NLRIRLGDLV TIHPCPDIKY ATRISVLPIA DTIEGITGNL FDVFLKPYFV
160 170 180 190 200
EAYRPVRKGD HFVVRGGMRQ VEFKVVDVEP EEYAVVAQDT IIHWEGEPIN
210 220 230 240 250
REDEENNMNE VGYDDIGGCR KQMAQIREMV ELPLRHPQLF KAIGIKPPRG
260 270 280 290 300
VLMYGPPGTG KTLMARAVAN ETGAFFFLIN GPEVMSKMAG ESESNLRKAF
310 320 330 340 350
EEAEKNAPAI IFIDEIDSIA PKRDKTNGEV ERRVVSQLLT LMDGMKARSN
360 370 380 390 400
VVVIAATNRP NSIDPALRRF GRFDREVDIG IPDATGRLEV LRIHTKNMKL
410 420 430 440 450
ADDVDLEALA AETHGYVGAD IASLCSEAAM QQIREKMDLI DLDEDEIDAE
460 470 480 490 500
VLDSLGVTMD NFRFALGNSN PSALRETVVE SVNVTWDDVG GLDEIKEELK
510 520 530 540 550
ETVEYPVLHP DQYTKFGLSP SKGVLFYGPP GTGKTLLAKA VATEVSANFI
560 570 580 590 600
SVKGPELLSM WYGESESNIR DIFDKARAAA PTVVFLDELD SIAKARGGSL
610 620 630 640 650
GDAGGASDRV VNQLLTEMDG MNAKKNVFVI GATNRPDQID PAILRPGRLD
660 670 680 690 700
QLIYVPLPDE NARLSILNAQ LRKTPLEPGL ELTAIAKATQ GFSGADLLYI
710 720 730 740 750
VQRAAKYAIK DSIEAHRQHE AEKEVKVEGE DVEMTDEGAK AEQEPEVDPV
760 770 780 790 800
PYITKEHFAE AMKTAKRSVS DAELRRYEAY SQQMKASRGQ FSNFNFNDAP
810 820 830
LGTTATDNAN SNNSAPSGAG AAFGSNAEED DDLYS
Length:835
Mass (Da):91,996
Last modified:October 1, 1996 - v3
Checksum:i02ADDB9A227614D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56956 Genomic DNA Translation: CAA40276.1
Z74174 Genomic DNA Translation: CAA98694.1
BK006938 Genomic DNA Translation: DAA11734.1
PIRiS67669
RefSeqiNP_010157.1, NM_001180185.1

Genome annotation databases

EnsemblFungiiYDL126C; YDL126C; YDL126C
GeneIDi851431
KEGGisce:YDL126C

Similar proteinsi

Entry informationi

Entry nameiCDC48_YEAST
AccessioniPrimary (citable) accession number: P25694
Secondary accession number(s): D6VRM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 189 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again