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Protein

Uricase

Gene

Uox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (Uox), Uricase (Uox)
  2. 5-hydroxyisourate hydrolase (Urah)
  3. 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (Urad)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei23Charge relay systemBy similarity1
Active sitei68Charge relay systemBy similarity1
Binding sitei170SubstrateBy similarity1
Binding sitei187SubstrateBy similarity1
Active sitei263Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • purine nucleobase metabolic process Source: MGI
  • urate catabolic process Source: MGI

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
Name:Uox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:98907 Uox

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001659862 – 303UricaseAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei10N6-acetyllysine; alternateCombined sources1
Modified residuei10N6-succinyllysine; alternateCombined sources1
Modified residuei23N6-acetyllysine; alternateCombined sources1
Modified residuei23N6-succinyllysine; alternateCombined sources1
Modified residuei27N6-acetyllysineCombined sources1
Modified residuei36N6-acetyllysineCombined sources1
Modified residuei39PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei122N6-acetyllysineCombined sources1
Modified residuei164N6-acetyllysineCombined sources1
Modified residuei175N6-acetyllysineCombined sources1
Modified residuei185N6-acetyllysineCombined sources1
Modified residuei220N6-acetyllysine; alternateCombined sources1
Modified residuei220N6-succinyllysine; alternateCombined sources1
Modified residuei227N6-acetyllysine; alternateCombined sources1
Modified residuei227N6-succinyllysine; alternateCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei277N6-acetyllysineCombined sources1
Modified residuei288PhosphotyrosineCombined sources1

Post-translational modificationi

Acetylation of Lys-118, Lys-164 and Lys-290 is observed in liver mitochondria from fasted mice but not from fed mice. May be deacetylated by Sirt5; however it is unclear whether Sirt5 mediates deacetylation or desuccinylation of Uox; additional evidence is required to validate these results (PubMed:23085393).2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP25688
PeptideAtlasiP25688
PRIDEiP25688

2D gel databases

SWISS-2DPAGEiP25688

PTM databases

iPTMnetiP25688
PhosphoSitePlusiP25688
SwissPalmiP25688

Expressioni

Gene expression databases

BgeeiENSMUSG00000028186
CleanExiMM_UOX
ExpressionAtlasiP25688 baseline and differential
GenevisibleiP25688 MM

Interactioni

Protein-protein interaction databases

IntActiP25688, 6 interactors
MINTiP25688
STRINGi10090.ENSMUSP00000029837

Structurei

3D structure databases

ProteinModelPortaliP25688
SMRiP25688
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 69Substrate bindingBy similarity2
Regioni234 – 235Substrate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi301 – 303Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

eggNOGiKOG1599 Eukaryota
COG3648 LUCA
GeneTreeiENSGT00390000010863
HOGENOMiHOG000250659
HOVERGENiHBG018060
InParanoidiP25688
KOiK00365
OMAiKDRCFAT
OrthoDBiEOG091G0E99
PhylomeDBiP25688
TreeFamiTF323438

Family and domain databases

InterProiView protein in InterPro
IPR002042 Uricase
IPR019842 Uricase_CS
PfamiView protein in Pfam
PF01014 Uricase, 2 hits
PIRSFiPIRSF000241 Urate_oxidase, 1 hit
PRINTSiPR00093 URICASE
TIGRFAMsiTIGR03383 urate_oxi, 1 hit
PROSITEiView protein in PROSITE
PS00366 URICASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHYHDNYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT
60 70 80 90 100
LRSKKDYLHG DNSDIIPTDT IKNTVHVLAK LRGIRNIETF AMNICEHFLS
110 120 130 140 150
SFNHVTRAHV YVEEVPWKRF EKNGIKHVHA FIHTPTGTHF CEVEQMRNGP
160 170 180 190 200
PVIHSGIKDL KVLKTTQSGF EGFLKDQFTT LPEVKDRCFA TQVYCKWRYQ
210 220 230 240 250
RRDVDFEAIW GAVRDIVLQK FAGPYDKGEY SPSVQKTLYD IQVLSLSQLP
260 270 280 290 300
EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVKRKLP

SRL
Length:303
Mass (Da):35,039
Last modified:January 23, 2007 - v2
Checksum:iF521383D05370FA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27695 mRNA Translation: AAA40538.1
BC019771 mRNA Translation: AAH19771.1
CCDSiCCDS17905.1
PIRiB36227
RefSeqiNP_033500.1, NM_009474.5
UniGeneiMm.10865

Genome annotation databases

EnsembliENSMUST00000029837; ENSMUSP00000029837; ENSMUSG00000028186
GeneIDi22262
KEGGimmu:22262
UCSCiuc008rrp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiURIC_MOUSE
AccessioniPrimary (citable) accession number: P25688
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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