UniProtKB - P25685 (DNJB1_HUMAN)
Protein
DnaJ homolog subfamily B member 1
Gene
DNAJB1
Organism
Homo sapiens (Human)
Status
Functioni
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).2 Publications
GO - Molecular functioni
- ATPase activator activity Source: UniProtKB
- ATPase binding Source: BHF-UCL
- cadherin binding Source: BHF-UCL
- chaperone binding Source: UniProtKB
- Hsp70 protein binding Source: BHF-UCL
- protein folding chaperone Source: Ensembl
- transcription corepressor activity Source: UniProtKB
- unfolded protein binding Source: UniProtKB
GO - Biological processi
- chaperone cofactor-dependent protein refolding Source: UniProtKB
- forebrain development Source: Ensembl
- negative regulation of inclusion body assembly Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- negative regulation of transcription from RNA polymerase II promoter in response to stress Source: UniProtKB
- positive regulation of ATPase activity Source: BHF-UCL
- regulation of cellular response to heat Source: Reactome
- response to unfolded protein Source: ProtInc
Keywordsi
Molecular function | Chaperone |
Biological process | Stress response |
Enzyme and pathway databases
PathwayCommonsi | P25685 |
Reactomei | R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) R-HSA-3371568, Attenuation phase R-HSA-3371571, HSF1-dependent transactivation R-HSA-5687128, MAPK6/MAPK4 signaling |
SIGNORi | P25685 |
Names & Taxonomyi
Protein namesi | Recommended name: DnaJ homolog subfamily B member 1Alternative name(s): DnaJ protein homolog 1 Heat shock 40 kDa protein 1 Short name: HSP40 Short name: Heat shock protein 40 Human DnaJ protein 1 Short name: hDj-1 |
Gene namesi | Name:DNAJB1 Synonyms:DNAJ1, HDJ1, HSPF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000132002.7 |
HGNCi | HGNC:5270, DNAJB1 |
MIMi | 604572, gene |
neXtProti | NX_P25685 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: BHF-UCL
- dendritic spine Source: Ensembl
- glutamatergic synapse Source: Ensembl
- neuronal cell body Source: Ensembl
- sperm head Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 3337 |
MalaCardsi | DNAJB1 |
OpenTargetsi | ENSG00000132002 |
Orphaneti | 401920, Fibrolamellar hepatocellular carcinoma |
PharmGKBi | PA27412 |
Miscellaneous databases
Pharosi | P25685, Tbio |
Polymorphism and mutation databases
BioMutai | DNAJB1 |
DMDMi | 1706473 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000071016 | 2 – 340 | DnaJ homolog subfamily B member 1Add BLAST | 339 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 307 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P25685 |
jPOSTi | P25685 |
MassIVEi | P25685 |
PaxDbi | P25685 |
PeptideAtlasi | P25685 |
PRIDEi | P25685 |
ProteomicsDBi | 5411 54280 [P25685-1] |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00015947 |
PTM databases
iPTMneti | P25685 |
PhosphoSitePlusi | P25685 |
Expressioni
Inductioni
By heat shock.1 Publication
Gene expression databases
Bgeei | ENSG00000132002, Expressed in hypothalamus and 251 other tissues |
ExpressionAtlasi | P25685, baseline and differential |
Genevisiblei | P25685, HS |
Organism-specific databases
HPAi | ENSG00000132002, Tissue enhanced (vagina) |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP25685
With | #Exp. | IntAct |
---|---|---|
TERF1 [P54274] | 2 | EBI-357034,EBI-710997 |
TTLL12 [Q14166] | 4 | EBI-357034,EBI-923010 |
Phlda1 [Q62392] from Mus musculus. | 2 | EBI-357034,EBI-309727 |
GO - Molecular functioni
- ATPase binding Source: BHF-UCL
- cadherin binding Source: BHF-UCL
- chaperone binding Source: UniProtKB
- Hsp70 protein binding Source: BHF-UCL
- unfolded protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109569, 133 interactors |
CORUMi | P25685 |
DIPi | DIP-41180N |
IntActi | P25685, 73 interactors |
MINTi | P25685 |
STRINGi | 9606.ENSP00000254322 |
Miscellaneous databases
RNActi | P25685, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P25685 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P25685 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 70 | JPROSITE-ProRule annotationAdd BLAST | 69 |
Phylogenomic databases
eggNOGi | KOG0714, Eukaryota |
GeneTreei | ENSGT00940000160312 |
HOGENOMi | CLU_017633_0_0_1 |
InParanoidi | P25685 |
OMAi | GRTMVVE |
PhylomeDBi | P25685 |
TreeFami | TF105141 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
IDEALi | IID00446 |
InterProi | View protein in InterPro IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR018253, DnaJ_domain_CS IPR008971, HSP40/DnaJ_pept-bd IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits |
PROSITEi | View protein in PROSITE PS00636, DNAJ_1, 1 hit PS50076, DNAJ_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P25685-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE
60 70 80 90 100
AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA
110 120 130 140 150
MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR
160 170 180 190 200
SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE
210 220 230 240 250
DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD
260 270 280 290 300
GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE
310 320 330 340
GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0QXK0 | M0QXK0_HUMAN | DnaJ homolog subfamily B member 1 | DNAJB1 | 162 | Annotation score: | ||
M0R080 | M0R080_HUMAN | DnaJ homolog subfamily B member 1 | DNAJB1 | 183 | Annotation score: | ||
M0QYT3 | M0QYT3_HUMAN | DnaJ homolog subfamily B member 1 | DNAJB1 | 116 | Annotation score: | ||
M0R1D6 | M0R1D6_HUMAN | DnaJ homolog subfamily B member 1 | DNAJB1 | 91 | Annotation score: | ||
M0QZD0 | M0QZD0_HUMAN | DnaJ homolog subfamily B member 1 | DNAJB1 | 107 | Annotation score: | ||
M0R128 | M0R128_HUMAN | DnaJ homolog subfamily B member 1 | DNAJB1 | 79 | Annotation score: | ||
A0A1B0GX60 | A0A1B0GX60_HUMAN | DnaJ homolog subfamily B member 1 | DNAJB1 | 80 | Annotation score: |
Sequence cautioni
The sequence CAA44287 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 68 | G → L in CAA44287 (PubMed:1754405).Curated | 1 | |
Sequence conflicti | 150 | R → C in CAA44287 (PubMed:1754405).Curated | 1 | |
Sequence conflicti | 183 | M → T in CAA44287 (PubMed:1754405).Curated | 1 | |
Sequence conflicti | 320 | V → A in CAA44287 (PubMed:1754405).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056414 | 1 – 100 | Missing in isoform 2. 1 PublicationAdd BLAST | 100 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62421 mRNA Translation: CAA44287.1 Frameshift. D49547 mRNA Translation: BAA08495.1 D85429 Genomic DNA Translation: BAA12819.1 AK301817 mRNA Translation: BAG63264.1 AC009004 Genomic DNA No translation available. AC012318 Genomic DNA No translation available. BC002352 mRNA Translation: AAH02352.1 BC019827 mRNA Translation: AAH19827.1 |
CCDSi | CCDS12312.1 [P25685-1] CCDS74295.1 [P25685-2] |
PIRi | JN0912 S20062 |
RefSeqi | NP_001287843.1, NM_001300914.1 [P25685-2] NP_001300893.1, NM_001313964.1 [P25685-2] NP_006136.1, NM_006145.2 [P25685-1] XP_006722796.1, XM_006722733.1 [P25685-2] XP_006722797.1, XM_006722734.3 [P25685-2] XP_011526258.1, XM_011527956.2 [P25685-2] |
Genome annotation databases
Ensembli | ENST00000254322; ENSP00000254322; ENSG00000132002 [P25685-1] ENST00000396969; ENSP00000444212; ENSG00000132002 [P25685-2] |
GeneIDi | 3337 |
KEGGi | hsa:3337 |
UCSCi | uc010xnr.2, human [P25685-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62421 mRNA Translation: CAA44287.1 Frameshift. D49547 mRNA Translation: BAA08495.1 D85429 Genomic DNA Translation: BAA12819.1 AK301817 mRNA Translation: BAG63264.1 AC009004 Genomic DNA No translation available. AC012318 Genomic DNA No translation available. BC002352 mRNA Translation: AAH02352.1 BC019827 mRNA Translation: AAH19827.1 |
CCDSi | CCDS12312.1 [P25685-1] CCDS74295.1 [P25685-2] |
PIRi | JN0912 S20062 |
RefSeqi | NP_001287843.1, NM_001300914.1 [P25685-2] NP_001300893.1, NM_001313964.1 [P25685-2] NP_006136.1, NM_006145.2 [P25685-1] XP_006722796.1, XM_006722733.1 [P25685-2] XP_006722797.1, XM_006722734.3 [P25685-2] XP_011526258.1, XM_011527956.2 [P25685-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HDJ | NMR | - | A | 1-76 | [»] | |
2QLD | X-ray | 2.70 | A | 158-340 | [»] | |
3AGX | X-ray | 1.85 | A/B | 161-340 | [»] | |
3AGY | X-ray | 1.85 | A/B | 161-340 | [»] | |
3AGZ | X-ray | 2.51 | A/B | 151-340 | [»] | |
4WB7 | X-ray | 1.90 | A/B | 2-70 | [»] | |
6BYR | X-ray | 3.66 | A/C | 2-70 | [»] | |
SMRi | P25685 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109569, 133 interactors |
CORUMi | P25685 |
DIPi | DIP-41180N |
IntActi | P25685, 73 interactors |
MINTi | P25685 |
STRINGi | 9606.ENSP00000254322 |
PTM databases
iPTMneti | P25685 |
PhosphoSitePlusi | P25685 |
Polymorphism and mutation databases
BioMutai | DNAJB1 |
DMDMi | 1706473 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00015947 |
Proteomic databases
EPDi | P25685 |
jPOSTi | P25685 |
MassIVEi | P25685 |
PaxDbi | P25685 |
PeptideAtlasi | P25685 |
PRIDEi | P25685 |
ProteomicsDBi | 5411 54280 [P25685-1] |
Protocols and materials databases
Antibodypediai | 26735, 797 antibodies |
DNASUi | 3337 |
Genome annotation databases
Ensembli | ENST00000254322; ENSP00000254322; ENSG00000132002 [P25685-1] ENST00000396969; ENSP00000444212; ENSG00000132002 [P25685-2] |
GeneIDi | 3337 |
KEGGi | hsa:3337 |
UCSCi | uc010xnr.2, human [P25685-1] |
Organism-specific databases
CTDi | 3337 |
DisGeNETi | 3337 |
EuPathDBi | HostDB:ENSG00000132002.7 |
GeneCardsi | DNAJB1 |
HGNCi | HGNC:5270, DNAJB1 |
HPAi | ENSG00000132002, Tissue enhanced (vagina) |
MalaCardsi | DNAJB1 |
MIMi | 604572, gene |
neXtProti | NX_P25685 |
OpenTargetsi | ENSG00000132002 |
Orphaneti | 401920, Fibrolamellar hepatocellular carcinoma |
PharmGKBi | PA27412 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0714, Eukaryota |
GeneTreei | ENSGT00940000160312 |
HOGENOMi | CLU_017633_0_0_1 |
InParanoidi | P25685 |
OMAi | GRTMVVE |
PhylomeDBi | P25685 |
TreeFami | TF105141 |
Enzyme and pathway databases
PathwayCommonsi | P25685 |
Reactomei | R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) R-HSA-3371568, Attenuation phase R-HSA-3371571, HSF1-dependent transactivation R-HSA-5687128, MAPK6/MAPK4 signaling |
SIGNORi | P25685 |
Miscellaneous databases
BioGRID-ORCSi | 3337, 23 hits in 823 CRISPR screens |
ChiTaRSi | DNAJB1, human |
EvolutionaryTracei | P25685 |
GeneWikii | DNAJB1 |
GenomeRNAii | 3337 |
Pharosi | P25685, Tbio |
PROi | PR:P25685 |
RNActi | P25685, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132002, Expressed in hypothalamus and 251 other tissues |
ExpressionAtlasi | P25685, baseline and differential |
Genevisiblei | P25685, HS |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
IDEALi | IID00446 |
InterProi | View protein in InterPro IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR018253, DnaJ_domain_CS IPR008971, HSP40/DnaJ_pept-bd IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits |
PROSITEi | View protein in PROSITE PS00636, DNAJ_1, 1 hit PS50076, DNAJ_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DNJB1_HUMAN | |
Accessioni | P25685Primary (citable) accession number: P25685 Secondary accession number(s): B4DX52 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 212 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM