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UniProtKB - P25685 (DNJB1_HUMAN)

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Protein

DnaJ homolog subfamily B member 1

Gene

DNAJB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).2 Publications

GO - Molecular functioni

  • ATPase activator activity Source: UniProtKB
  • ATPase binding Source: BHF-UCL
  • cadherin binding Source: BHF-UCL
  • chaperone binding Source: UniProtKB
  • Hsp70 protein binding Source: BHF-UCL
  • RNA polymerase II transcription corepressor activity Source: UniProtKB
  • unfolded protein binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • chaperone cofactor-dependent protein refolding Source: UniProtKB
  • forebrain development Source: Ensembl
  • negative regulation of inclusion body assembly Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter in response to stress Source: UniProtKB
  • positive regulation of ATPase activity Source: BHF-UCL
  • regulation of cellular response to heat Source: Reactome
  • response to unfolded protein Source: ProtInc
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionChaperone
Biological processStress response

Enzyme and pathway databases

ReactomeiR-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-3371568 Attenuation phase
R-HSA-3371571 HSF1-dependent transactivation
R-HSA-5687128 MAPK6/MAPK4 signaling
SIGNORiP25685

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 1
Alternative name(s):
DnaJ protein homolog 1
Heat shock 40 kDa protein 1
Short name:
HSP40
Short name:
Heat shock protein 40
Human DnaJ protein 1
Short name:
hDj-1
Gene namesi
Name:DNAJB1
Synonyms:DNAJ1, HDJ1, HSPF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000132002.7
HGNCiHGNC:5270 DNAJB1
MIMi604572 gene
neXtProtiNX_P25685

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3337
MalaCardsiDNAJB1
OpenTargetsiENSG00000132002
Orphaneti401920 Fibrolamellar hepatocellular carcinoma
PharmGKBiPA27412

Polymorphism and mutation databases

BioMutaiDNAJB1
DMDMi1706473

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000710162 – 340DnaJ homolog subfamily B member 1Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei307PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP25685
PaxDbiP25685
PeptideAtlasiP25685
PRIDEiP25685
ProteomicsDBi54280

2D gel databases

REPRODUCTION-2DPAGEiIPI00015947

PTM databases

iPTMnetiP25685
PhosphoSitePlusiP25685

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

BgeeiENSG00000132002
CleanExiHS_DNAJB1
ExpressionAtlasiP25685 baseline and differential
GenevisibleiP25685 HS

Organism-specific databases

HPAiCAB017450
HPA063247

Interactioni

Subunit structurei

Interacts with DNAJC3 (PubMed:9920933). Interacts with HSF1 (via transactivation domain); this interaction results in the inhibition of heat shock- and HSF1-induced transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL
  • cadherin binding Source: BHF-UCL
  • chaperone binding Source: UniProtKB
  • Hsp70 protein binding Source: BHF-UCL
  • unfolded protein binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi109569, 108 interactors
CORUMiP25685
DIPiDIP-41180N
IntActiP25685, 52 interactors
MINTiP25685
STRINGi9606.ENSP00000254322

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 9Combined sources5
Helixi17 – 31Combined sources15
Turni33 – 35Combined sources3
Helixi41 – 55Combined sources15
Helixi58 – 70Combined sources13
Beta strandi166 – 170Combined sources5
Helixi172 – 177Combined sources6
Beta strandi179 – 190Combined sources12
Turni191 – 193Combined sources3
Beta strandi197 – 208Combined sources12
Beta strandi217 – 220Combined sources4
Beta strandi228 – 230Combined sources3
Beta strandi235 – 241Combined sources7
Beta strandi248 – 250Combined sources3
Beta strandi253 – 261Combined sources9
Helixi262 – 267Combined sources6
Beta strandi269 – 274Combined sources6
Beta strandi280 – 285Combined sources6
Beta strandi294 – 297Combined sources4
Beta strandi305 – 307Combined sources3
Beta strandi314 – 321Combined sources8
Helixi328 – 337Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HDJNMR-A1-76[»]
2QLDX-ray2.70A158-340[»]
3AGXX-ray1.85A/B161-340[»]
3AGYX-ray1.85A/B161-340[»]
3AGZX-ray2.51A/B151-340[»]
4WB7X-ray1.90A/B2-70[»]
ProteinModelPortaliP25685
SMRiP25685
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25685

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 70JPROSITE-ProRule annotationAdd BLAST69

Phylogenomic databases

eggNOGiKOG0714 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00760000118947
HOGENOMiHOG000226718
HOVERGENiHBG066727
InParanoidiP25685
KOiK09507
OMAiGAYTYQF
OrthoDBiEOG091G0DLS
PhylomeDBiP25685
TreeFamiTF105141

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 2 hits
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P25685-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE 
        60         70         80         90        100
AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA 
       110        120        130        140        150
MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR 
       160        170        180        190        200
SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE 
       210        220        230        240        250
DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD 
       260        270        280        290        300
GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE 
       310        320        330        340
GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
Length:340
Mass (Da):38,044
Last modified:January 23, 2007 - v4
Checksum:i17545098B0C196DF
GO
Isoform 2 (identifier: P25685-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Note: No experimental confirmation available.
Show »
Length:240
Mass (Da):27,016
Checksum:i760FFA819B48174B
GO

Sequence cautioni

The sequence CAA44287 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68G → L in CAA44287 (PubMed:1754405).Curated1
Sequence conflicti150R → C in CAA44287 (PubMed:1754405).Curated1
Sequence conflicti183M → T in CAA44287 (PubMed:1754405).Curated1
Sequence conflicti320V → A in CAA44287 (PubMed:1754405).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564141 – 100Missing in isoform 2. 1 PublicationAdd BLAST100

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62421 mRNA Translation: CAA44287.1 Frameshift.
D49547 mRNA Translation: BAA08495.1
D85429 Genomic DNA Translation: BAA12819.1
AK301817 mRNA Translation: BAG63264.1
AC009004 Genomic DNA No translation available.
AC012318 Genomic DNA No translation available.
BC002352 mRNA Translation: AAH02352.1
BC019827 mRNA Translation: AAH19827.1
CCDSiCCDS12312.1 [P25685-1]
CCDS74295.1 [P25685-2]
PIRiJN0912
S20062
RefSeqiNP_001287843.1, NM_001300914.1 [P25685-2]
NP_001300893.1, NM_001313964.1 [P25685-2]
NP_006136.1, NM_006145.2 [P25685-1]
XP_006722796.1, XM_006722733.1 [P25685-2]
XP_006722797.1, XM_006722734.3 [P25685-2]
XP_011526258.1, XM_011527956.2 [P25685-2]
UniGeneiHs.515210

Genome annotation databases

EnsembliENST00000254322; ENSP00000254322; ENSG00000132002 [P25685-1]
ENST00000396969; ENSP00000444212; ENSG00000132002 [P25685-2]
GeneIDi3337
KEGGihsa:3337
UCSCiuc010xnr.2 human [P25685-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDNJB1_HUMAN
AccessioniPrimary (citable) accession number: P25685
Secondary accession number(s): B4DX52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 192 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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