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Protein

DnaJ homolog subfamily B member 1

Gene

DNAJB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).2 Publications

GO - Molecular functioni

  • ATPase activator activity Source: UniProtKB
  • ATPase binding Source: BHF-UCL
  • cadherin binding Source: BHF-UCL
  • chaperone binding Source: UniProtKB
  • Hsp70 protein binding Source: BHF-UCL
  • transcription corepressor activity Source: UniProtKB
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response

Enzyme and pathway databases

ReactomeiR-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-3371568 Attenuation phase
R-HSA-3371571 HSF1-dependent transactivation
R-HSA-5687128 MAPK6/MAPK4 signaling
SIGNORiP25685

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 1
Alternative name(s):
DnaJ protein homolog 1
Heat shock 40 kDa protein 1
Short name:
HSP40
Short name:
Heat shock protein 40
Human DnaJ protein 1
Short name:
hDj-1
Gene namesi
Name:DNAJB1
Synonyms:DNAJ1, HDJ1, HSPF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000132002.7
HGNCiHGNC:5270 DNAJB1
MIMi604572 gene
neXtProtiNX_P25685

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3337
MalaCardsiDNAJB1
OpenTargetsiENSG00000132002
Orphaneti401920 Fibrolamellar hepatocellular carcinoma
PharmGKBiPA27412

Polymorphism and mutation databases

BioMutaiDNAJB1
DMDMi1706473

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000710162 – 340DnaJ homolog subfamily B member 1Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei307PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP25685
PaxDbiP25685
PeptideAtlasiP25685
PRIDEiP25685
ProteomicsDBi54280

2D gel databases

REPRODUCTION-2DPAGEiIPI00015947

PTM databases

iPTMnetiP25685
PhosphoSitePlusiP25685

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

BgeeiENSG00000132002 Expressed in 239 organ(s), highest expression level in hypothalamus
CleanExiHS_DNAJB1
ExpressionAtlasiP25685 baseline and differential
GenevisibleiP25685 HS

Organism-specific databases

HPAiCAB017450
HPA063247

Interactioni

Subunit structurei

Interacts with DNAJC3 (PubMed:9920933). Interacts with HSF1 (via transactivation domain); this interaction results in the inhibition of heat shock- and HSF1-induced transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401).2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109569, 108 interactors
CORUMiP25685
DIPiDIP-41180N
IntActiP25685, 52 interactors
MINTiP25685
STRINGi9606.ENSP00000254322

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP25685
SMRiP25685
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25685

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 70JPROSITE-ProRule annotationAdd BLAST69

Phylogenomic databases

eggNOGiKOG0714 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00760000118947
HOGENOMiHOG000226718
HOVERGENiHBG066727
InParanoidiP25685
KOiK09507
OMAiGAYTYQF
OrthoDBiEOG091G0DLS
PhylomeDBiP25685
TreeFamiTF105141

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 2 hits
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P25685-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE
60 70 80 90 100
AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA
110 120 130 140 150
MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR
160 170 180 190 200
SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE
210 220 230 240 250
DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD
260 270 280 290 300
GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE
310 320 330 340
GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
Length:340
Mass (Da):38,044
Last modified:January 23, 2007 - v4
Checksum:i17545098B0C196DF
GO
Isoform 2 (identifier: P25685-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Note: No experimental confirmation available.
Show »
Length:240
Mass (Da):27,016
Checksum:i760FFA819B48174B
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R080M0R080_HUMAN
DnaJ homolog subfamily B member 1
DNAJB1
183Annotation score:
M0QXK0M0QXK0_HUMAN
DnaJ homolog subfamily B member 1
DNAJB1
162Annotation score:
M0QYT3M0QYT3_HUMAN
DnaJ homolog subfamily B member 1
DNAJB1
116Annotation score:
M0QZD0M0QZD0_HUMAN
DnaJ homolog subfamily B member 1
DNAJB1
107Annotation score:
M0R1D6M0R1D6_HUMAN
DnaJ homolog subfamily B member 1
DNAJB1
91Annotation score:
M0R128M0R128_HUMAN
DnaJ homolog subfamily B member 1
DNAJB1
79Annotation score:
A0A1B0GX60A0A1B0GX60_HUMAN
DnaJ homolog subfamily B member 1
DNAJB1
80Annotation score:

Sequence cautioni

The sequence CAA44287 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68G → L in CAA44287 (PubMed:1754405).Curated1
Sequence conflicti150R → C in CAA44287 (PubMed:1754405).Curated1
Sequence conflicti183M → T in CAA44287 (PubMed:1754405).Curated1
Sequence conflicti320V → A in CAA44287 (PubMed:1754405).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564141 – 100Missing in isoform 2. 1 PublicationAdd BLAST100

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62421 mRNA Translation: CAA44287.1 Frameshift.
D49547 mRNA Translation: BAA08495.1
D85429 Genomic DNA Translation: BAA12819.1
AK301817 mRNA Translation: BAG63264.1
AC009004 Genomic DNA No translation available.
AC012318 Genomic DNA No translation available.
BC002352 mRNA Translation: AAH02352.1
BC019827 mRNA Translation: AAH19827.1
CCDSiCCDS12312.1 [P25685-1]
CCDS74295.1 [P25685-2]
PIRiJN0912
S20062
RefSeqiNP_001287843.1, NM_001300914.1 [P25685-2]
NP_001300893.1, NM_001313964.1 [P25685-2]
NP_006136.1, NM_006145.2 [P25685-1]
XP_006722796.1, XM_006722733.1 [P25685-2]
XP_006722797.1, XM_006722734.3 [P25685-2]
XP_011526258.1, XM_011527956.2 [P25685-2]
UniGeneiHs.515210

Genome annotation databases

EnsembliENST00000254322; ENSP00000254322; ENSG00000132002 [P25685-1]
ENST00000396969; ENSP00000444212; ENSG00000132002 [P25685-2]
GeneIDi3337
KEGGihsa:3337
UCSCiuc010xnr.2 human [P25685-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62421 mRNA Translation: CAA44287.1 Frameshift.
D49547 mRNA Translation: BAA08495.1
D85429 Genomic DNA Translation: BAA12819.1
AK301817 mRNA Translation: BAG63264.1
AC009004 Genomic DNA No translation available.
AC012318 Genomic DNA No translation available.
BC002352 mRNA Translation: AAH02352.1
BC019827 mRNA Translation: AAH19827.1
CCDSiCCDS12312.1 [P25685-1]
CCDS74295.1 [P25685-2]
PIRiJN0912
S20062
RefSeqiNP_001287843.1, NM_001300914.1 [P25685-2]
NP_001300893.1, NM_001313964.1 [P25685-2]
NP_006136.1, NM_006145.2 [P25685-1]
XP_006722796.1, XM_006722733.1 [P25685-2]
XP_006722797.1, XM_006722734.3 [P25685-2]
XP_011526258.1, XM_011527956.2 [P25685-2]
UniGeneiHs.515210

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HDJNMR-A1-76[»]
2QLDX-ray2.70A158-340[»]
3AGXX-ray1.85A/B161-340[»]
3AGYX-ray1.85A/B161-340[»]
3AGZX-ray2.51A/B151-340[»]
4WB7X-ray1.90A/B2-70[»]
ProteinModelPortaliP25685
SMRiP25685
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109569, 108 interactors
CORUMiP25685
DIPiDIP-41180N
IntActiP25685, 52 interactors
MINTiP25685
STRINGi9606.ENSP00000254322

PTM databases

iPTMnetiP25685
PhosphoSitePlusiP25685

Polymorphism and mutation databases

BioMutaiDNAJB1
DMDMi1706473

2D gel databases

REPRODUCTION-2DPAGEiIPI00015947

Proteomic databases

EPDiP25685
PaxDbiP25685
PeptideAtlasiP25685
PRIDEiP25685
ProteomicsDBi54280

Protocols and materials databases

DNASUi3337
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254322; ENSP00000254322; ENSG00000132002 [P25685-1]
ENST00000396969; ENSP00000444212; ENSG00000132002 [P25685-2]
GeneIDi3337
KEGGihsa:3337
UCSCiuc010xnr.2 human [P25685-1]

Organism-specific databases

CTDi3337
DisGeNETi3337
EuPathDBiHostDB:ENSG00000132002.7
GeneCardsiDNAJB1
H-InvDBiHIX0014838
HGNCiHGNC:5270 DNAJB1
HPAiCAB017450
HPA063247
MalaCardsiDNAJB1
MIMi604572 gene
neXtProtiNX_P25685
OpenTargetsiENSG00000132002
Orphaneti401920 Fibrolamellar hepatocellular carcinoma
PharmGKBiPA27412
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0714 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00760000118947
HOGENOMiHOG000226718
HOVERGENiHBG066727
InParanoidiP25685
KOiK09507
OMAiGAYTYQF
OrthoDBiEOG091G0DLS
PhylomeDBiP25685
TreeFamiTF105141

Enzyme and pathway databases

ReactomeiR-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-3371568 Attenuation phase
R-HSA-3371571 HSF1-dependent transactivation
R-HSA-5687128 MAPK6/MAPK4 signaling
SIGNORiP25685

Miscellaneous databases

ChiTaRSiDNAJB1 human
EvolutionaryTraceiP25685
GeneWikiiDNAJB1
GenomeRNAii3337
PROiPR:P25685
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132002 Expressed in 239 organ(s), highest expression level in hypothalamus
CleanExiHS_DNAJB1
ExpressionAtlasiP25685 baseline and differential
GenevisibleiP25685 HS

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 2 hits
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDNJB1_HUMAN
AccessioniPrimary (citable) accession number: P25685
Secondary accession number(s): B4DX52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 194 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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