The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P25665 (METE_ECOLI)
Protein
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Gene
metE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
UniRule annotationMiscellaneous
Has an absolute requirement for a polyglutamylated folate as substrate. Its activity depends on phosphate anions and divalent cations.
Catalytic activityi
- 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamateUniRule annotationEC:2.1.1.14UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
: L-methionine biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).UniRule annotation This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 117 | 5-methyltetrahydropteroyltri-L-glutamateUniRule annotation | 1 | |
Binding sitei | 484 | L-homocysteineUniRule annotation | 1 | |
Binding sitei | 484 | L-methionineUniRule annotation | 1 | |
Binding sitei | 561 | 5-methyltetrahydropteroyltri-L-glutamateUniRule annotation | 1 | |
Binding sitei | 599 | L-homocysteineUniRule annotation | 1 | |
Binding sitei | 599 | L-methionineUniRule annotation | 1 | |
Binding sitei | 605 | 5-methyltetrahydropteroyltri-L-glutamateUniRule annotation | 1 | |
Metal bindingi | 641 | Zinc; catalytic; via tele nitrogenUniRule annotation | 1 | |
Metal bindingi | 643 | Zinc; catalyticUniRule annotation | 1 | |
Metal bindingi | 665 | Zinc; catalyticUniRule annotation | 1 | |
Active sitei | 694 | Proton donorUniRule annotation | 1 | |
Metal bindingi | 726 | Zinc; catalyticUniRule annotation | 1 |
GO - Molecular functioni
- 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Source: EcoCyc
- methionine synthase activity Source: BHF-UCL
- zinc ion binding Source: InterPro
GO - Biological processi
- homocysteine metabolic process Source: BHF-UCL
- methionine biosynthetic process Source: BHF-UCL
- methylation Source: UniProtKB-KW
- tetrahydrofolate interconversion Source: BHF-UCL
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:HOMOCYSMET-MONOMER |
BRENDAi | 2.1.1.14, 2026 |
UniPathwayi | UPA00051;UER00082 |
Names & Taxonomyi
Protein namesi | Recommended name: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseUniRule annotation (EC:2.1.1.14UniRule annotation)Alternative name(s): Cobalamin-independent methionine synthaseUniRule annotation Methionine synthase, vitamin-B12 independent isozymeUniRule annotation |
Gene namesi | Name:metEUniRule annotation Ordered Locus Names:b3829, JW3805 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 641 | H → N: Impaired activity, lower affinity for zinc binding. 1 Publication | 1 | |
Mutagenesisi | 641 | H → Q: Impaired activity, lower affinity for zinc binding. Binds homocysteine 2-4x more weakly than wild-type. 1 Publication | 1 | |
Mutagenesisi | 643 | C → S: Impaired activity, lower affinity for zinc binding. Binds homocysteine 7x tighter than wild-type. 1 Publication | 1 | |
Mutagenesisi | 726 | C → S: Impaired activity, lower affinity for zinc binding. Binds homocysteine 2-4x more weakly than wild-type. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000098630 | 2 – 753 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST | 752 |
Proteomic databases
jPOSTi | P25665 |
PaxDbi | P25665 |
PRIDEi | P25665 |
Interactioni
Subunit structurei
Monomer.
Binary interactionsi
P25665
With | #Exp. | IntAct |
---|---|---|
uvrD [P03018] | 2 | EBI-551247,EBI-559573 |
Protein-protein interaction databases
BioGRIDi | 4259477, 17 interactors 852620, 1 interactor |
DIPi | DIP-6847N |
IntActi | P25665, 12 interactors |
STRINGi | 511145.b3829 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 17 – 20 | 5-methyltetrahydropteroyltri-L-glutamate bindingUniRule annotation | 4 | |
Regioni | 431 – 433 | L-homocysteine bindingUniRule annotation | 3 | |
Regioni | 431 – 433 | L-methionine bindingUniRule annotation | 3 | |
Regioni | 515 – 516 | 5-methyltetrahydropteroyltri-L-glutamate bindingUniRule annotation | 2 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG0620, Bacteria |
HOGENOMi | CLU_013175_0_0_6 |
InParanoidi | P25665 |
OMAi | KVMKGML |
PhylomeDBi | P25665 |
Family and domain databases
CDDi | cd03311, CIMS_C_terminal_like, 1 hit |
Gene3Di | 3.20.20.210, 2 hits |
HAMAPi | MF_00172, Meth_synth, 1 hit |
InterProi | View protein in InterPro IPR013215, Cbl-indep_Met_Synth_N IPR006276, Cobalamin-indep_Met_synthase IPR002629, Met_Synth_C/arc IPR038071, UROD/MetE-like_sf |
Pfami | View protein in Pfam PF08267, Meth_synt_1, 1 hit PF01717, Meth_synt_2, 1 hit |
PIRSFi | PIRSF000382, MeTrfase_B12_ind, 1 hit |
SUPFAMi | SSF51726, SSF51726, 2 hits |
TIGRFAMsi | TIGR01371, met_syn_B12ind, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P25665-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTILNHTLGF PRVGLRRELK KAQESYWAGN STREELLAVG RELRARHWDQ
60 70 80 90 100
QKQAGIDLLP VGDFAWYDHV LTTSLLLGNV PARHQNKDGS VDIDTLFRIG
110 120 130 140 150
RGRAPTGEPA AAAEMTKWFN TNYHYMVPEF VKGQQFKLTW TQLLDEVDEA
160 170 180 190 200
LALGHKVKPV LLGPVTWLWL GKVKGEQFDR LSLLNDILPV YQQVLAELAK
210 220 230 240 250
RGIEWVQIDE PALVLELPQA WLDAYKPAYD ALQGQVKLLL TTYFEGVTPN
260 270 280 290 300
LDTITALPVQ GLHVDLVHGK DDVAELHKRL PSDWLLSAGL INGRNVWRAD
310 320 330 340 350
LTEKYAQIKD IVGKRDLWVA SSCSLLHSPI DLSVETRLDA EVKSWFAFAL
360 370 380 390 400
QKCHELALLR DALNSGDTAA LAEWSAPIQA RRHSTRVHNP AVEKRLAAIT
410 420 430 440 450
AQDSQRANVY EVRAEAQRAR FKLPAWPTTT IGSFPQTTEI RTLRLDFKKG
460 470 480 490 500
NLDANNYRTG IAEHIKQAIV EQERLGLDVL VHGEAERNDM VEYFGEHLDG
510 520 530 540 550
FVFTQNGWVQ SYGSRCVKPP IVIGDISRPA PITVEWAKYA QSLTDKPVKG
560 570 580 590 600
MLTGPVTILC WSFPREDVSR ETIAKQIALA LRDEVADLEA AGIGIIQIDE
610 620 630 640 650
PALREGLPLR RSDWDAYLQW GVEAFRINAA VAKDDTQIHT HMCYCEFNDI
660 670 680 690 700
MDSIAALDAD VITIETSRSD MELLESFEEF DYPNEIGPGV YDIHSPNVPS
710 720 730 740 750
VEWIEALLKK AAKRIPAERL WVNPDCGLKT RGWPETRAAL ANMVQAAQNL
RRG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 363 | L → V in AAA23544 (PubMed:1339288).Curated | 1 | |
Sequence conflicti | 605 | E → Q in AAA67625 (PubMed:8346018).Curated | 1 | |
Sequence conflicti | 659 | A → R in AAA67625 (PubMed:8346018).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87625 Genomic DNA Translation: AAA23544.1 M87049 Genomic DNA Translation: AAA67625.1 U00096 Genomic DNA Translation: AAC76832.1 AP009048 Genomic DNA Translation: BAE77472.1 J04155 Genomic DNA Translation: AAA24160.1 |
PIRi | F65187, A42863 |
RefSeqi | NP_418273.1, NC_000913.3 WP_000153907.1, NZ_SSZK01000046.1 |
Genome annotation databases
EnsemblBacteriai | AAC76832; AAC76832; b3829 BAE77472; BAE77472; BAE77472 |
GeneIDi | 948323 |
KEGGi | ecj:JW3805 eco:b3829 |
PATRICi | fig|1411691.4.peg.2879 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87625 Genomic DNA Translation: AAA23544.1 M87049 Genomic DNA Translation: AAA67625.1 U00096 Genomic DNA Translation: AAC76832.1 AP009048 Genomic DNA Translation: BAE77472.1 J04155 Genomic DNA Translation: AAA24160.1 |
PIRi | F65187, A42863 |
RefSeqi | NP_418273.1, NC_000913.3 WP_000153907.1, NZ_SSZK01000046.1 |
3D structure databases
AlphaFoldDBi | P25665 |
SMRi | P25665 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259477, 17 interactors 852620, 1 interactor |
DIPi | DIP-6847N |
IntActi | P25665, 12 interactors |
STRINGi | 511145.b3829 |
Proteomic databases
jPOSTi | P25665 |
PaxDbi | P25665 |
PRIDEi | P25665 |
Genome annotation databases
EnsemblBacteriai | AAC76832; AAC76832; b3829 BAE77472; BAE77472; BAE77472 |
GeneIDi | 948323 |
KEGGi | ecj:JW3805 eco:b3829 |
PATRICi | fig|1411691.4.peg.2879 |
Organism-specific databases
EchoBASEi | EB0579 |
Phylogenomic databases
eggNOGi | COG0620, Bacteria |
HOGENOMi | CLU_013175_0_0_6 |
InParanoidi | P25665 |
OMAi | KVMKGML |
PhylomeDBi | P25665 |
Enzyme and pathway databases
UniPathwayi | UPA00051;UER00082 |
BioCyci | EcoCyc:HOMOCYSMET-MONOMER |
BRENDAi | 2.1.1.14, 2026 |
Miscellaneous databases
PROi | PR:P25665 |
Family and domain databases
CDDi | cd03311, CIMS_C_terminal_like, 1 hit |
Gene3Di | 3.20.20.210, 2 hits |
HAMAPi | MF_00172, Meth_synth, 1 hit |
InterProi | View protein in InterPro IPR013215, Cbl-indep_Met_Synth_N IPR006276, Cobalamin-indep_Met_synthase IPR002629, Met_Synth_C/arc IPR038071, UROD/MetE-like_sf |
Pfami | View protein in Pfam PF08267, Meth_synt_1, 1 hit PF01717, Meth_synt_2, 1 hit |
PIRSFi | PIRSF000382, MeTrfase_B12_ind, 1 hit |
SUPFAMi | SSF51726, SSF51726, 2 hits |
TIGRFAMsi | TIGR01371, met_syn_B12ind, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | METE_ECOLI | |
Accessioni | P25665Primary (citable) accession number: P25665 Secondary accession number(s): Q2M8D4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 192 of the entry and version 6 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families