Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 159 (02 Jun 2021)
Sequence version 2 (15 Jul 1999)
Previous versions | rss
Add a publicationFeedback
Protein

Mediator of RNA polymerase II transcription subunit 12

Gene

SRB8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5.

4 Publications

Miscellaneous

Present with 1730 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 12
Alternative name(s):
Mediator complex subunit 12
Suppressor of RNA polymerase B 8
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SRB8
Synonyms:GIG1, MED12, NUT6, SSN5
Ordered Locus Names:YCR081W
ORF Names:YCR81W/YCR80W
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome III

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000000677, SRB8

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YCR081W

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000721831 – 1427Mediator of RNA polymerase II transcription subunit 12Add BLAST1427

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P25648

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P25648

PRoteomics IDEntifications database

More...
PRIDEi
P25648

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P25648

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme.

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
31054, 150 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-1853, CKM complex

Database of interacting proteins

More...
DIPi
DIP-1018N

Protein interaction database and analysis system

More...
IntActi
P25648, 14 interactors

Molecular INTeraction database

More...
MINTi
P25648

STRING: functional protein association networks

More...
STRINGi
4932.YCR081W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P25648, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P25648

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1042 – 1066DisorderedSequence analysisAdd BLAST25

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Mediator complex subunit 12 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG4522, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_256280_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P25648

Identification of Orthologs from Complete Genome Data

More...
OMAi
HGLKRRQ

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR019035, Mediator_Med12

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF09497, Med12, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01281, Med12, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P25648-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNGSGRYLL TPPDDLHPYV PSSKPQEQVY PDFKPWEHTA AEDQILANFV
60 70 80 90 100
AKGFYHTPMV NFESISARSS VHESLVTQSN ILSQQFDKII KIREDHINKI
110 120 130 140 150
PSNSTTTLHG PGFQLPNRIT LTDHRKETWL HELSSSHTSL VKIGKFIPHG
160 170 180 190 200
LKRRQVIEQC YLKFIPLKRA IWLIKCCYFI EWKSNHKKKR SNAAGADDAI
210 220 230 240 250
SMHLLKDWTD TFVYILEKLI FDMTNHYNDS QQLRTWKRQI SYFLKLLGNC
260 270 280 290 300
YSLRLINKEI FHHWLVEFIN KMENFEFLPL SLHILMIFWN DICQIDTNAP
310 320 330 340 350
VAATITSSQK EPFFLVTKIT DMLLHKYYIV SSSKSMINDE NYIINDIKKN
360 370 380 390 400
NKIKLNILKI LSSLILKIFQ EQSLEVFIFP TSNWEIYKPL LFEIVSNADT
410 420 430 440 450
NQNSDMKKKL ELISYRNESL KNNSSIRNVI MSASNANDFQ LTIVTCKQFP
460 470 480 490 500
KLSCIQLNCI DTQFTKLLDD NPTEFDWPTY VDQNPLTMHK IIQLILWSIH
510 520 530 540 550
PSRQFDHYES NQLVAKLLLL RINSTDEDLH EFQIEDAIWS LVFQLAKNFS
560 570 580 590 600
AQKRVVSYMM PSLYRLLNIL ITYGIIKVPT YIRKLISSGL LYLQDSNDKF
610 620 630 640 650
VHVQLLINLK ISPLMKSQYN MVLRNVMEYD VKFYEIFNFD QLVEITEQIK
660 670 680 690 700
MRILSNDITN LQLSKTPLSI KIMVAEWYLS HLCSGILSSV NRTVLLKIFK
710 720 730 740 750
IFCIDLEVFH HFFKWIEFIV YHQLLSDIES LEALMDILLC YQKLFSQFIN
760 770 780 790 800
DHILFTKTFI FIYKKVLKEK DVPAYNVTSF MPFWKFFMKN FPFVLKVDND
810 820 830 840 850
LRIELQSVYN DEKLKTEKLK NDKSEVLKVY SMINNSNQAV GQTWNFPEVF
860 870 880 890 900
QVNIRFLLHN SEIIDTNTSK QFQKARNNVM LLIATNLKEY NKFMSIFLKR
910 920 930 940 950
KDFTNKNLIQ LISLKLLTFE VTQNVLGLEY IIRLLPINLE NNDGSYGLFL
960 970 980 990 1000
KYHKEQFIKS NFEKILLTCY ELEKKYHGNE CEINYYEILL KILITYGSSP
1010 1020 1030 1040 1050
KLLATSTKII MLLLNDSVEN SSNILEDILY YSTCPSETDL NDIPLGSGQP
1060 1070 1080 1090 1100
DNDTVVTNDD KSDDDDHTVD EIDHVEYYVM MDFANLWVFQ AFTCFCIKKI
1110 1120 1130 1140 1150
MENNEPAMAM EDLKNFIFQI IEITNSNDLC SQIFDQLKDM QTIEMITQIV
1160 1170 1180 1190 1200
EKDFCTSCLQ NNNQKIDDNY IVVVIEIITS LSMRFQRETS GMIVISMENY
1210 1220 1230 1240 1250
HLLIKIIRQL SELNEGNLSK REIQIDAVLK IFSFHQDSIF QRIIADLSAD
1260 1270 1280 1290 1300
KPTSPFIDSI CKLFDKISFN LRLKLFLYEI LSSLKSFAIY SSTIDAPAFH
1310 1320 1330 1340 1350
TSGKVELPKK LLNLPPFQVS SFVKETKLHS GDYGEEEDAD QEESFSLNLG
1360 1370 1380 1390 1400
IGIVEIAHEN EQKWLIYDKK DHKYVCTFSM EPYHFISNYN TKYTDDMATG
1410 1420
SNDTTAFNDS CVNLSLFDAR FERKNPH
Length:1,427
Mass (Da):166,861
Last modified:July 15, 1999 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i776A2A9D82E331FE
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42268.1
BK006937 Genomic DNA Translation: DAA07551.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S74293

NCBI Reference Sequences

More...
RefSeqi
NP_010004.1, NM_001178787.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YCR081W_mRNA; YCR081W; YCR081W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
850442

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YCR081W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42268.1
BK006937 Genomic DNA Translation: DAA07551.1
PIRiS74293
RefSeqiNP_010004.1, NM_001178787.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
7KPVelectron microscopy3.80C1-1427[»]
7KPWelectron microscopy4.90C1-1427[»]
7KPXelectron microscopy4.40C1-1427[»]
SMRiP25648
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi31054, 150 interactors
ComplexPortaliCPX-1853, CKM complex
DIPiDIP-1018N
IntActiP25648, 14 interactors
MINTiP25648
STRINGi4932.YCR081W

PTM databases

iPTMnetiP25648

Proteomic databases

MaxQBiP25648
PaxDbiP25648
PRIDEiP25648

Genome annotation databases

EnsemblFungiiYCR081W_mRNA; YCR081W; YCR081W
GeneIDi850442
KEGGisce:YCR081W

Organism-specific databases

SGDiS000000677, SRB8
VEuPathDBiFungiDB:YCR081W

Phylogenomic databases

eggNOGiKOG4522, Eukaryota
HOGENOMiCLU_256280_0_0_1
InParanoidiP25648
OMAiHGLKRRQ

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P25648
RNActiP25648, protein

Family and domain databases

InterProiView protein in InterPro
IPR019035, Mediator_Med12
PfamiView protein in Pfam
PF09497, Med12, 1 hit
SMARTiView protein in SMART
SM01281, Med12, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSRB8_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P25648
Secondary accession number(s): D6VR82, P25647
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 15, 1999
Last modified: June 2, 2021
This is version 159 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again