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UniProtKB - P25641 (ATG15_YEAST)
Protein
Putative lipase ATG15
Gene
ATG15
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension.
7 PublicationsMiscellaneous
Present with 3060 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.1.1.3
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 332 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- phospholipase activity Source: SGD
- triglyceride lipase activity Source: UniProtKB-EC
GO - Biological processi
- autophagy Source: UniProtKB
- macroautophagy Source: GO_Central
- membrane disassembly Source: UniProtKB
- multivesicular body membrane disassembly Source: SGD
- neutral lipid catabolic process Source: SGD
- pexophagy Source: SGD
- phosphatidylserine catabolic process Source: SGD
- piecemeal microautophagy of the nucleus Source: SGD
- vacuolar protein processing Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Autophagy, Lipid degradation, Lipid metabolism |
Protein family/group databases
ESTHERi | yeast-ATG15, Lipase_3 |
TCDBi | 8.A.178.1.1, the lipase atg15 (atg15) family |
Names & Taxonomyi
Protein namesi | Recommended name: Putative lipase ATG15 (EC:3.1.1.3)Alternative name(s): Autophagy-related protein 15 Cytoplasm to vacuole targeting protein 17 |
Gene namesi | Name:ATG15 Synonyms:AUT5, CVT17 Ordered Locus Names:YCR068W ORF Names:YCR68W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000664, ATG15 |
VEuPathDBi | FungiDB:YCR068W |
Subcellular locationi
Endosome
- multivesicular body membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Other locations
- Prevacuolar compartment membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC).1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Endosome
- multivesicular body membrane Source: UniProtKB-SubCell
Vacuole
- vacuolar lumen Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 14 | Cytoplasmic1 PublicationAdd BLAST | 14 | |
Transmembranei | 15 – 35 | Helical; Signal-anchor for type II membrane proteinAdd BLAST | 21 | |
Topological domaini | 36 – 520 | Lumenal1 PublicationAdd BLAST | 485 |
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 332 | S → A: Loss of function. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090372 | 1 – 520 | Putative lipase ATG15Add BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 173 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 202 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 208 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylated.3 Publications
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | P25641 |
PaxDbi | P25641 |
Interactioni
Subunit structurei
Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).
Protein-protein interaction databases
BioGRIDi | 31044, 318 interactors |
DIPi | DIP-5001N |
IntActi | P25641, 7 interactors |
MINTi | P25641 |
STRINGi | 4932.YCR068W |
Miscellaneous databases
RNActi | P25641, protein |
Structurei
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4540, Eukaryota |
HOGENOMi | CLU_028295_0_2_1 |
InParanoidi | P25641 |
OMAi | WFGCKDE |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002921, Fungal_lipase-like |
Pfami | View protein in Pfam PF01764, Lipase_3, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00120, LIPASE_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
P25641-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLHKSPSRKR FASPLHLGCI LTLTVLCLIA YYFALPDYLS VGKSSSRGAM
60 70 80 90 100
DQKSDGTFRL KSIYRHGVGA NHRLHQRLEV TPEVISAAGM LYQETTTQGQ
110 120 130 140 150
DFEDQEPLWT TNAEYATTNP FDFEFELRRM PLLMKRMKER DPEFIESYIY
160 170 180 190 200
GETYMTEEEE HAMWIDDDIV APNITDRGTV VSLALMSSNA YVRIPQTGDW
210 220 230 240 250
RNVTEPWNET EPEDFGWDGD GIRGHVFYNE VENIVVLSIK GTSAQGLPGS
260 270 280 290 300
GEDETTGNDK INDNLLFSCC CARVSYLWTT VCDCYVKSYI CDESCLEKEL
310 320 330 340 350
RRKDRFYSAV VDIYKGVLKE YPDAAIWVTG HSLGGALASL LGRTFGLPAV
360 370 380 390 400
AFESPGELLP SKRLHLPFPP GLPSYMEGIW HFGHNADPIF MGTCNGASSS
410 420 430 440 450
CSLVGYAMET ACHTGRVCVY DVVNDKGWSV NMFNHRIHKV IDEVLLGYEQ
460 470 480 490 500
AAKCVEPEPC VDCYNWKFIP SRDWESSSRL ITKTKSHAAP TTTTRTTATT
510 520
TSSSTCVGRN WLGFCTKYEL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59720 Genomic DNA Translation: CAC42987.1 BK006937 Genomic DNA Translation: DAA07540.1 |
PIRi | S19483 S74292 |
RefSeqi | NP_009994.2, NM_001178779.1 |
Genome annotation databases
EnsemblFungii | YCR068W_mRNA; YCR068W; YCR068W |
GeneIDi | 850432 |
KEGGi | sce:YCR068W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59720 Genomic DNA Translation: CAC42987.1 BK006937 Genomic DNA Translation: DAA07540.1 |
PIRi | S19483 S74292 |
RefSeqi | NP_009994.2, NM_001178779.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 31044, 318 interactors |
DIPi | DIP-5001N |
IntActi | P25641, 7 interactors |
MINTi | P25641 |
STRINGi | 4932.YCR068W |
Protein family/group databases
ESTHERi | yeast-ATG15, Lipase_3 |
TCDBi | 8.A.178.1.1, the lipase atg15 (atg15) family |
Proteomic databases
MaxQBi | P25641 |
PaxDbi | P25641 |
Genome annotation databases
EnsemblFungii | YCR068W_mRNA; YCR068W; YCR068W |
GeneIDi | 850432 |
KEGGi | sce:YCR068W |
Organism-specific databases
SGDi | S000000664, ATG15 |
VEuPathDBi | FungiDB:YCR068W |
Phylogenomic databases
eggNOGi | KOG4540, Eukaryota |
HOGENOMi | CLU_028295_0_2_1 |
InParanoidi | P25641 |
OMAi | WFGCKDE |
Miscellaneous databases
PROi | PR:P25641 |
RNActi | P25641, protein |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002921, Fungal_lipase-like |
Pfami | View protein in Pfam PF01764, Lipase_3, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00120, LIPASE_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG15_YEAST | |
Accessioni | P25641Primary (citable) accession number: P25641 Secondary accession number(s): D6VR71, Q8NIL6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | October 24, 2003 | |
Last modified: | February 23, 2022 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names - SIMILARITY comments
Index of protein domains and families