Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA polymerase IV

Gene

POL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Repair polymerase. Involved in gap-filling in DNA nonhomologous end joining (NHEJ) required for double-strand break repair. Seems to conduct DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Preferentially acts upon short gaps formed by the alignment of linear duplexes with complementary single-strand ends. Required for filling gaps that need removal of a 5'- or 3'-terminal mismatch, however lacks nuclease activities.3 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarity

Activity regulationi

Stimulated by the interaction with the DNL4-LIF1 complex.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi367MagnesiumBy similarity1
Metal bindingi369MagnesiumBy similarity1
Metal bindingi502MagnesiumBy similarity1

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed DNA polymerase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29329-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IV (EC:2.7.7.7)
Short name:
POL IV
Gene namesi
Name:POL4
Synonyms:POLX
Ordered Locus Names:YCR014C
ORF Names:YCR14C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

SGDiS000000607 POL4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi247K → R: Weakened DNA-binding. 1 Publication1
Mutagenesisi248K → R: Weakened DNA-binding. 1 Publication1
Mutagenesisi367D → E: Loss of nucleotidyl transfer. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002187892 – 582DNA polymerase IVAdd BLAST581

Proteomic databases

PaxDbiP25615
PRIDEiP25615

PTM databases

iPTMnetiP25615

Expressioni

Inductioni

During meiosis.1 Publication

Interactioni

Subunit structurei

Interacts with DNL4 subunit of the DNL4-LIF1 complex.1 Publication

Protein-protein interaction databases

BioGridi30992, 79 interactors
DIPiDIP-4228N
IntActiP25615, 10 interactors
MINTiP25615
STRINGi4932.YCR014C

Structurei

3D structure databases

ProteinModelPortaliP25615
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni360 – 369Involved in ssDNA bindingBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi472 – 478Poly-Glu7

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated

Phylogenomic databases

HOGENOMiHOG000057123
InParanoidiP25615
KOiK10981
OMAiCKWSELG
OrthoDBiEOG092C2OBE

Family and domain databases

CDDicd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
InterProiView protein in InterPro
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR002008 DNA_pol_X_beta-like
IPR029398 PolB_thumb
PfamiView protein in Pfam
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF14716 HHH_8, 1 hit
PRINTSiPR00869 DNAPOLX
PR00870 DNAPOLXBETA
SMARTiView protein in SMART
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
PROSITEiView protein in PROSITE
PS00522 DNA_POLYMERASE_X, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25615-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLKGKFFAF LPNPNTSSNK FFKSILEKKG ATIVSSIQNC LQSSRKEVVI
60 70 80 90 100
LIEDSFVDSD MHLTQKDIFQ REAGLNDVDE FLGKIEQSGI QCVKTSCITK
110 120 130 140 150
WVQNDKFAFQ KDDLIKFQPS IIVISDNADD GQSSTDKESE ISTDVESERN
160 170 180 190 200
DDSNNKDMIQ ASKPLKRLLQ GDKGRASLVT DKTKYKNNEL IIGALKRLTK
210 220 230 240 250
KYEIEGEKFR ARSYRLAKQS MENCDFNVRS GEEAHTKLRN IGPSIAKKIQ
260 270 280 290 300
VILDTGVLPG LNDSVGLEDK LKYFKNCYGI GSEIAKRWNL LNFESFCVAA
310 320 330 340 350
KKDPEEFVSD WTILFGWSYY DDWLCKMSRN ECFTHLKKVQ KALRGIDPEC
360 370 380 390 400
QVELQGSYNR GYSKCGDIDL LFFKPFCNDT TELAKIMETL CIKLYKDGYI
410 420 430 440 450
HCFLQLTPNL EKLFLKRIVE RFRTAKIVGY GERKRWYSSE IIKKFFMGVK
460 470 480 490 500
LSPRELEELK EMKNDEGTLL IEEEEEEETK LKPIDQYMSL NAKDGNYCRR
510 520 530 540 550
LDFFCCKWDE LGAGRIHYTG SKEYNRWIRI LAAQKGFKLT QHGLFRNNIL
560 570 580
LESFNERRIF ELLNLKYAEP EHRNIEWEKK TA
Length:582
Mass (Da):67,527
Last modified:January 23, 2007 - v4
Checksum:i63BD59061F4864D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42331.2
BK006937 Genomic DNA Translation: DAA07491.1
PIRiS19424
RefSeqiNP_009940.2, NM_001178727.1

Genome annotation databases

EnsemblFungiiCAA42331; CAA42331; CAA42331
YCR014C; YCR014C; YCR014C
GeneIDi850372
KEGGisce:YCR014C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42331.2
BK006937 Genomic DNA Translation: DAA07491.1
PIRiS19424
RefSeqiNP_009940.2, NM_001178727.1

3D structure databases

ProteinModelPortaliP25615
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30992, 79 interactors
DIPiDIP-4228N
IntActiP25615, 10 interactors
MINTiP25615
STRINGi4932.YCR014C

PTM databases

iPTMnetiP25615

Proteomic databases

PaxDbiP25615
PRIDEiP25615

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42331; CAA42331; CAA42331
YCR014C; YCR014C; YCR014C
GeneIDi850372
KEGGisce:YCR014C

Organism-specific databases

SGDiS000000607 POL4

Phylogenomic databases

HOGENOMiHOG000057123
InParanoidiP25615
KOiK10981
OMAiCKWSELG
OrthoDBiEOG092C2OBE

Enzyme and pathway databases

BioCyciYEAST:G3O-29329-MONOMER

Miscellaneous databases

PROiPR:P25615

Family and domain databases

CDDicd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
InterProiView protein in InterPro
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR002008 DNA_pol_X_beta-like
IPR029398 PolB_thumb
PfamiView protein in Pfam
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF14716 HHH_8, 1 hit
PRINTSiPR00869 DNAPOLX
PR00870 DNAPOLXBETA
SMARTiView protein in SMART
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
PROSITEiView protein in PROSITE
PS00522 DNA_POLYMERASE_X, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPO4_YEAST
AccessioniPrimary (citable) accession number: P25615
Secondary accession number(s): D6VR22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 161 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again