UniProtKB - P25588 (MRC1_YEAST)
Protein
Mediator of replication checkpoint protein 1
Gene
MRC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for normal DNA replication. Phosphorylated in response to DNA replication stress. Phosphorylation allows it to mediate the activation of RAD53.2 Publications
Miscellaneous
Present with 721 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- chromatin silencing at telomere Source: SGD
- DNA repair Source: SGD
- DNA replication Source: SGD
- DNA replication checkpoint Source: SGD
- intra-S DNA damage checkpoint Source: SGD
- maintenance of DNA repeat elements Source: SGD
- mitotic sister chromatid cohesion Source: SGD
- regulation of nuclear cell cycle DNA replication Source: SGD
- replication fork protection Source: SGD
- silent mating-type cassette heterochromatin assembly Source: SGD
- telomere maintenance Source: SGD
Keywordsi
Biological process | DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of replication checkpoint protein 1Alternative name(s): DNA replication checkpoint mediator MRC1 |
Gene namesi | Name:MRC1 Ordered Locus Names:YCL061C ORF Names:YCL61C/YCL60C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YCL061C |
SGDi | S000000566, MRC1 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Associated with chromatin during S phase.
Nucleus
- nuclear periphery Source: SGD
- nuclear replication fork Source: SGD
- nucleus Source: SGD
- replication fork protection complex Source: SGD
Other locations
- chromosome, telomeric region Source: GOC
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096574 | 1 – 1096 | Mediator of replication checkpoint protein 1Add BLAST | 1096 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 144 | PhosphoserineCombined sources | 1 | |
Modified residuei | 409 | PhosphoserineCombined sources | 1 | |
Modified residuei | 411 | PhosphoserineCombined sources | 1 | |
Modified residuei | 434 | PhosphoserineCombined sources | 1 | |
Modified residuei | 605 | PhosphoserineCombined sources | 1 | |
Modified residuei | 607 | PhosphoserineCombined sources | 1 | |
Modified residuei | 609 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 801 | PhosphoserineCombined sources | 1 | |
Modified residuei | 807 | PhosphoserineCombined sources | 1 | |
Modified residuei | 911 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by MEC1 and RAD53.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P25588 |
PaxDbi | P25588 |
PRIDEi | P25588 |
PTM databases
CarbonylDBi | P25588 |
iPTMneti | P25588 |
Interactioni
Subunit structurei
Interacts with CDC45 in S phase.
1 PublicationBinary interactionsi
Hide detailsP25588
With | #Exp. | IntAct |
---|---|---|
CDC45 [Q08032] | 4 | EBI-412442,EBI-4292 |
DIA2 [Q08496] | 11 | EBI-412442,EBI-31943 |
HOG1 [P32485] | 4 | EBI-412442,EBI-8437 |
TOF1 [P53840] | 4 | EBI-412442,EBI-28257 |
Protein-protein interaction databases
BioGRIDi | 30926, 461 interactors |
DIPi | DIP-1017N |
IntActi | P25588, 19 interactors |
MINTi | P25588 |
STRINGi | 4932.YCL061C |
Miscellaneous databases
RNActi | P25588, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 488 – 542 | Sequence analysisAdd BLAST | 55 | |
Coiled coili | 652 – 716 | Sequence analysisAdd BLAST | 65 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QSP5, Eukaryota |
HOGENOMi | CLU_007004_0_0_1 |
InParanoidi | P25588 |
OMAi | EDEWHGI |
Family and domain databases
InterProi | View protein in InterPro IPR018564, Repl_chkpnt_MRC1_dom |
Pfami | View protein in Pfam PF09444, MRC1, 1 hit |
i Sequence
Sequence statusi: Complete.
P25588-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDDALHALSS LTAKKRTTTY KKVAVPILDE NDNTNGNGPN DIDNPPELTG
60 70 80 90 100
NGFLFANATL NRVKNRLEGK KAPEQNHNNG KDRSENSLPT QLISNLYDGG
110 120 130 140 150
EELEKSEVKD NSYSEKNVSS SFTQTQRIPV SIQQDKVFNV PIHSVNDGKP
160 170 180 190 200
TQLIKEDGLV NETSQALKTP LTTGRPGATQ RIDSSGATSQ TQPIKSIEPQ
210 220 230 240 250
SQIITTSSNH SNALSPKIPI IPTELIGTSP LFQSIQNRGP DTQMDVPPQT
260 270 280 290 300
AHDEDKTQAI GIPQATHQEQ KTQIDTVAQT LQDEVPHTLK IREIQSELAS
310 320 330 340 350
EDSKREKARN VEYKKPQKPI PTKKFFSKES FLADFDDSSS NEDDDIKLEN
360 370 380 390 400
AHPKPVQNDD ELHENKSVEL NLTDETRINE KRVPLLSSYA NNLKREIDSS
410 420 430 440 450
KCITLDLDSD SDEYGDDDMD SIKLSKDESV LPISQLSKAT ILNLKARLSK
460 470 480 490 500
QNQKLSQRPN KSKDPKVDHN VLLNTLRKAS RKQILDHQKE VIETKGLKLE
510 520 530 540 550
DMAKEKEIVE NLLEQEILRN KRIRQKEKRR EKLEENDFQL NAHDSGSDSG
560 570 580 590 600
SESSGFALSG NEIADYESSG SENDNRRESD SEKEDDEIIL KQKKSHHVKH
610 620 630 640 650
IINESDSDTE VEAKPKEKAD ESLPKRIAIN LGHYGDNIGE DTDKFQETNV
660 670 680 690 700
LDTQNIEEVM AERNTIENEV KDDVYVNEEA DEAIRRQLID KEKLQLKQKE
710 720 730 740 750
KEHEAKIKEL KKRGVTNFFE MEAEESEDEW HGIGGADGEG SDDYDSDLEK
760 770 780 790 800
MIDDYSKNNF NPHEIREMLA AENKEMDIKM INKILYDIKN GGFRNKRAKN
810 820 830 840 850
SLELELSDDD EDDVLQQYRL KRRELMRKRR LEIGDDAKLV KNPKSSAFFE
860 870 880 890 900
SMVEDIIEYK NPFGAEEEYN LDITSTATDL DTQDNSINVG DNTGNNEQKP
910 920 930 940 950
VDQKNKKVII SEDFVQKSLS FLKSNNYEDF ETDKELSRIQ HGNDEAIEDL
960 970 980 990 1000
YTLKQNSSIK SFTNSQTDST TSKTVNTIID LEKRPEDEDE VENGDTSLVG
1010 1020 1030 1040 1050
VFKHPSIIKS FASRTDINDK FKEGNKTVKI LKSYKTVGSS KASITYMGKT
1060 1070 1080 1090
RKLIAPKRKT EGSHRYHHDH HNKKMKMKTK TKSNKLFESG QDSFDN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 748 | L → V in CAA37945 (PubMed:2169608).Curated | 1 | |
Sequence conflicti | 808 | Missing in CAA37945 (PubMed:2169608).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59720 Genomic DNA Translation: CAC42953.1 X53998 Genomic DNA Translation: CAA37945.1 BK006937 Genomic DNA Translation: DAA07425.1 |
PIRi | S74279 |
RefSeqi | NP_009871.2, NM_001178704.1 |
Genome annotation databases
EnsemblFungii | YCL061C_mRNA; YCL061C; YCL061C |
GeneIDi | 850297 |
KEGGi | sce:YCL061C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59720 Genomic DNA Translation: CAC42953.1 X53998 Genomic DNA Translation: CAA37945.1 BK006937 Genomic DNA Translation: DAA07425.1 |
PIRi | S74279 |
RefSeqi | NP_009871.2, NM_001178704.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 30926, 461 interactors |
DIPi | DIP-1017N |
IntActi | P25588, 19 interactors |
MINTi | P25588 |
STRINGi | 4932.YCL061C |
PTM databases
CarbonylDBi | P25588 |
iPTMneti | P25588 |
Proteomic databases
MaxQBi | P25588 |
PaxDbi | P25588 |
PRIDEi | P25588 |
Genome annotation databases
EnsemblFungii | YCL061C_mRNA; YCL061C; YCL061C |
GeneIDi | 850297 |
KEGGi | sce:YCL061C |
Organism-specific databases
EuPathDBi | FungiDB:YCL061C |
SGDi | S000000566, MRC1 |
Phylogenomic databases
eggNOGi | ENOG502QSP5, Eukaryota |
HOGENOMi | CLU_007004_0_0_1 |
InParanoidi | P25588 |
OMAi | EDEWHGI |
Miscellaneous databases
PROi | PR:P25588 |
RNActi | P25588, protein |
Family and domain databases
InterProi | View protein in InterPro IPR018564, Repl_chkpnt_MRC1_dom |
Pfami | View protein in Pfam PF09444, MRC1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MRC1_YEAST | |
Accessioni | P25588Primary (citable) accession number: P25588 Secondary accession number(s): D6VQV6 Q8NIN2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | September 19, 2003 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names