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UniProtKB - P25588 (MRC1_YEAST)

Entry version 167 (02 Jun 2021)
Sequence version 3 (19 Sep 2003)
Previous versions | rss
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Protein

Mediator of replication checkpoint protein 1

Gene

MRC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Required for normal DNA replication. Phosphorylated in response to DNA replication stress. Phosphorylation allows it to mediate the activation of RAD53.

2 Publications

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processDNA replication

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mediator of replication checkpoint protein 1
Alternative name(s):
DNA replication checkpoint mediator MRC1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MRC1
Ordered Locus Names:YCL061C
ORF Names:YCL61C/YCL60C
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome III

Organism-specific databases

Saccharomyces Genome Database

More...SGDi		S000000566, MRC1

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		FungiDB:YCL061C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000965741 – 1096Mediator of replication checkpoint protein 1Add BLAST1096

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei144PhosphoserineCombined sources1
Modified residuei409PhosphoserineCombined sources1
Modified residuei411PhosphoserineCombined sources1
Modified residuei434PhosphoserineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei607PhosphoserineCombined sources1
Modified residuei609PhosphothreonineCombined sources1
Modified residuei801PhosphoserineCombined sources1
Modified residuei807PhosphoserineCombined sources1
Modified residuei911PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by MEC1 and RAD53.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...MaxQBi		P25588

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P25588

PRoteomics IDEntifications database

More...PRIDEi		P25588

PTM databases

CarbonylDB database of protein carbonylation sites

More...CarbonylDBi		P25588

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P25588

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with CDC45 in S phase.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		30926, 461 interactors

Database of interacting proteins

More...DIPi		DIP-1017N

Protein interaction database and analysis system

More...IntActi		P25588, 19 interactors

Molecular INTeraction database

More...MINTi		P25588

STRING: functional protein association networks

More...STRINGi		4932.YCL061C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P25588, protein

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni68 – 90DisorderedSequence analysisAdd BLAST23
Regioni166 – 200DisorderedSequence analysisAdd BLAST35
Regioni294 – 316DisorderedSequence analysisAdd BLAST23
Regioni336 – 365DisorderedSequence analysisAdd BLAST30
Regioni527 – 620DisorderedSequence analysisAdd BLAST94
Regioni724 – 743DisorderedSequence analysisAdd BLAST20
Regioni881 – 903DisorderedSequence analysisAdd BLAST23
Regioni1058 – 1096DisorderedSequence analysisAdd BLAST39

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili488 – 542Sequence analysisAdd BLAST55
Coiled coili652 – 716Sequence analysisAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi344 – 365Basic and acidic residuesSequence analysisAdd BLAST22
Compositional biasi527 – 541Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi542 – 568Polar residuesSequence analysisAdd BLAST27
Compositional biasi569 – 620Basic and acidic residuesSequence analysisAdd BLAST52
Compositional biasi881 – 899Polar residuesSequence analysisAdd BLAST19
Compositional biasi1063 – 1080Basic residuesSequence analysisAdd BLAST18

Keywords - Domaini

Coiled coil

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502QSP5, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_007004_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P25588

Identification of Orthologs from Complete Genome Data

More...OMAi		EDEWHGI

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR018564, Repl_chkpnt_MRC1_dom

Pfam protein domain database

More...Pfami		View protein in Pfam
PF09444, MRC1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P25588-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDALHALSS LTAKKRTTTY KKVAVPILDE NDNTNGNGPN DIDNPPELTG 
        60         70         80         90        100
NGFLFANATL NRVKNRLEGK KAPEQNHNNG KDRSENSLPT QLISNLYDGG 
       110        120        130        140        150
EELEKSEVKD NSYSEKNVSS SFTQTQRIPV SIQQDKVFNV PIHSVNDGKP 
       160        170        180        190        200
TQLIKEDGLV NETSQALKTP LTTGRPGATQ RIDSSGATSQ TQPIKSIEPQ 
       210        220        230        240        250
SQIITTSSNH SNALSPKIPI IPTELIGTSP LFQSIQNRGP DTQMDVPPQT 
       260        270        280        290        300
AHDEDKTQAI GIPQATHQEQ KTQIDTVAQT LQDEVPHTLK IREIQSELAS 
       310        320        330        340        350
EDSKREKARN VEYKKPQKPI PTKKFFSKES FLADFDDSSS NEDDDIKLEN 
       360        370        380        390        400
AHPKPVQNDD ELHENKSVEL NLTDETRINE KRVPLLSSYA NNLKREIDSS 
       410        420        430        440        450
KCITLDLDSD SDEYGDDDMD SIKLSKDESV LPISQLSKAT ILNLKARLSK 
       460        470        480        490        500
QNQKLSQRPN KSKDPKVDHN VLLNTLRKAS RKQILDHQKE VIETKGLKLE 
       510        520        530        540        550
DMAKEKEIVE NLLEQEILRN KRIRQKEKRR EKLEENDFQL NAHDSGSDSG 
       560        570        580        590        600
SESSGFALSG NEIADYESSG SENDNRRESD SEKEDDEIIL KQKKSHHVKH 
       610        620        630        640        650
IINESDSDTE VEAKPKEKAD ESLPKRIAIN LGHYGDNIGE DTDKFQETNV 
       660        670        680        690        700
LDTQNIEEVM AERNTIENEV KDDVYVNEEA DEAIRRQLID KEKLQLKQKE 
       710        720        730        740        750
KEHEAKIKEL KKRGVTNFFE MEAEESEDEW HGIGGADGEG SDDYDSDLEK 
       760        770        780        790        800
MIDDYSKNNF NPHEIREMLA AENKEMDIKM INKILYDIKN GGFRNKRAKN 
       810        820        830        840        850
SLELELSDDD EDDVLQQYRL KRRELMRKRR LEIGDDAKLV KNPKSSAFFE 
       860        870        880        890        900
SMVEDIIEYK NPFGAEEEYN LDITSTATDL DTQDNSINVG DNTGNNEQKP 
       910        920        930        940        950
VDQKNKKVII SEDFVQKSLS FLKSNNYEDF ETDKELSRIQ HGNDEAIEDL 
       960        970        980        990       1000
YTLKQNSSIK SFTNSQTDST TSKTVNTIID LEKRPEDEDE VENGDTSLVG 
      1010       1020       1030       1040       1050
VFKHPSIIKS FASRTDINDK FKEGNKTVKI LKSYKTVGSS KASITYMGKT 
      1060       1070       1080       1090 
RKLIAPKRKT EGSHRYHHDH HNKKMKMKTK TKSNKLFESG QDSFDN
Length:1,096
Mass (Da):124,326
Last modified:September 19, 2003 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i378345EE503FFA81
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti748L → V in CAA37945 (PubMed:2169608).Curated1
Sequence conflicti808Missing in CAA37945 (PubMed:2169608).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X59720 Genomic DNA Translation: CAC42953.1
X53998 Genomic DNA Translation: CAA37945.1
BK006937 Genomic DNA Translation: DAA07425.1

Protein sequence database of the Protein Information Resource

More...PIRi		S74279

NCBI Reference Sequences

More...RefSeqi		NP_009871.2, NM_001178704.1

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		YCL061C_mRNA; YCL061C; YCL061C

Database of genes from NCBI RefSeq genomes

More...GeneIDi		850297

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		sce:YCL061C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAC42953.1
X53998 Genomic DNA Translation: CAA37945.1
BK006937 Genomic DNA Translation: DAA07425.1
PIRiS74279
RefSeqiNP_009871.2, NM_001178704.1

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi30926, 461 interactors
DIPiDIP-1017N
IntActiP25588, 19 interactors
MINTiP25588
STRINGi4932.YCL061C

PTM databases

CarbonylDBiP25588
iPTMnetiP25588

Proteomic databases

MaxQBiP25588
PaxDbiP25588
PRIDEiP25588

Genome annotation databases

EnsemblFungiiYCL061C_mRNA; YCL061C; YCL061C
GeneIDi850297
KEGGisce:YCL061C

Organism-specific databases

SGDiS000000566, MRC1
VEuPathDBiFungiDB:YCL061C

Phylogenomic databases

eggNOGiENOG502QSP5, Eukaryota
HOGENOMiCLU_007004_0_0_1
InParanoidiP25588
OMAiEDEWHGI

Miscellaneous databases

Protein Ontology

More...PROi		PR:P25588
RNActiP25588, protein

Family and domain databases

InterProiView protein in InterPro
IPR018564, Repl_chkpnt_MRC1_dom
PfamiView protein in Pfam
PF09444, MRC1, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMRC1_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P25588
Secondary accession number(s): D6VQV6
, P25589, P27513, P87003, Q07218, Q8NIN2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 19, 2003
Last modified: June 2, 2021
This is version 167 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names
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