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UniProtKB - P25549 (ASLA_ECOLI)
Protein
Putative sulfatase AslA
Gene
aslA
Organism
Escherichia coli (strain K12)
Status
Functioni
No E.coli strains have been observed to have an arylsulfatase activity. A 'latent' activity has been observed by heterologous expression of a genomic region linked to tynA, but it does not map near aslA.
1 PublicationCofactori
Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 94 | CalciumBy similarity | 1 | |
Metal bindingi | 95 | CalciumBy similarity | 1 | |
Active sitei | 136 | NucleophileBy similarity | 1 | |
Metal bindingi | 136 | Calcium; via 3-oxoalanineBy similarity | 1 | |
Active sitei | 190 | By similarity | 1 | |
Metal bindingi | 356 | CalciumBy similarity | 1 | |
Metal bindingi | 357 | CalciumBy similarity | 1 |
GO - Molecular functioni
- arylsulfatase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ARYLSULFAT-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aslA1 Publication Synonyms:atsA1 Publication Ordered Locus Names:b3801, JW3773 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm Curated
Other locations
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000033446 | 25 – 551 | Putative sulfatase AslAAdd BLAST | 527 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 136 | 3-oxoalanine (Ser)By similarity | 1 |
Post-translational modificationi
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity
Proteomic databases
PaxDbi | P25549 |
PRIDEi | P25549 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262605, 5 interactors |
IntActi | P25549, 1 interactor |
STRINGi | 511145.b3801 |
Family & Domainsi
Sequence similaritiesi
Belongs to the sulfatase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3119, Bacteria |
HOGENOMi | CLU_006332_10_0_6 |
InParanoidi | P25549 |
OMAi | PALEPCC |
PhylomeDBi | P25549 |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR024607, Sulfatase_CS IPR000917, Sulfatase_N |
Pfami | View protein in Pfam PF00884, Sulfatase, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00523, SULFATASE_1, 1 hit PS00149, SULFATASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P25549-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEFSFSPKRL VVAVAAALPL MASAADTPST ATARKGFAGY DHPNQYLVKP
60 70 80 90 100
ATTIADNMMP VMQHPAQDKE TQQKLAELEK KTGKKPNVVV FLLDDVGWMD
110 120 130 140 150
VGFNGGGVAV GNPTPDIDAV ASQGLILTSA YSQPSSSPTR ATILTGQYSI
160 170 180 190 200
HHGILMPPMY GQPGGLQGLT TLPQLLHDQG YVTQAIGKWH MGENKESQPQ
210 220 230 240 250
NVGFDDFRGF NSVSDMYTEW RDVHVNPEVA LSPDRSEYIK QLPFSKDDVH
260 270 280 290 300
AVRGGEQQAI ADITPKYMED LDQRWMDYGV KFLDKMAKSD KPFFLYYGTR
310 320 330 340 350
GCHFDNYPNA KYAGSSPART SYGDCMVEMN DVFANLYKTL EKNGQLDNTL
360 370 380 390 400
IVFTSDNGPE AEVPPHGRTP FRGAKGSTWE GGVRVPTFVY WKGMIQPRKS
410 420 430 440 450
DGIVDLADLF PTALDLAGHP GAKVANLVPK TTFIDGVDQT SFFLGTNGQS
460 470 480 490 500
NRKAEHYFLN GKLAAVRMDE FKYHVLIQQP YAYTQSGYQG GFTGTVMQTA
510 520 530 540 550
GSSVFNLYTD PQESDSIGVR HIPMGVPLQT EMHAYMEILK KYPPRAQIKS
D
Sequence cautioni
The sequence AAC32036 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 191 | M → I in AAC32036 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90498 Genomic DNA Translation: AAC32036.1 Frameshift. M87049 Genomic DNA Translation: AAA67597.1 U00096 Genomic DNA Translation: AAC76804.1 AP009048 Genomic DNA Translation: BAE77500.1 |
PIRi | S30691 |
RefSeqi | NP_418245.1, NC_000913.3 WP_000395863.1, NZ_SSZK01000025.1 |
Genome annotation databases
EnsemblBacteriai | AAC76804; AAC76804; b3801 BAE77500; BAE77500; BAE77500 |
GeneIDi | 949015 |
KEGGi | ecj:JW3773 eco:b3801 |
PATRICi | fig|1411691.4.peg.2908 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90498 Genomic DNA Translation: AAC32036.1 Frameshift. M87049 Genomic DNA Translation: AAA67597.1 U00096 Genomic DNA Translation: AAC76804.1 AP009048 Genomic DNA Translation: BAE77500.1 |
PIRi | S30691 |
RefSeqi | NP_418245.1, NC_000913.3 WP_000395863.1, NZ_SSZK01000025.1 |
3D structure databases
AlphaFoldDBi | P25549 |
SMRi | P25549 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262605, 5 interactors |
IntActi | P25549, 1 interactor |
STRINGi | 511145.b3801 |
Proteomic databases
PaxDbi | P25549 |
PRIDEi | P25549 |
Genome annotation databases
EnsemblBacteriai | AAC76804; AAC76804; b3801 BAE77500; BAE77500; BAE77500 |
GeneIDi | 949015 |
KEGGi | ecj:JW3773 eco:b3801 |
PATRICi | fig|1411691.4.peg.2908 |
Organism-specific databases
EchoBASEi | EB0087 |
Phylogenomic databases
eggNOGi | COG3119, Bacteria |
HOGENOMi | CLU_006332_10_0_6 |
InParanoidi | P25549 |
OMAi | PALEPCC |
PhylomeDBi | P25549 |
Enzyme and pathway databases
BioCyci | EcoCyc:ARYLSULFAT-MONOMER |
Miscellaneous databases
PROi | PR:P25549 |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR024607, Sulfatase_CS IPR000917, Sulfatase_N |
Pfami | View protein in Pfam PF00884, Sulfatase, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00523, SULFATASE_1, 1 hit PS00149, SULFATASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ASLA_ECOLI | |
Accessioni | P25549Primary (citable) accession number: P25549 Secondary accession number(s): Q2M8A6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families