UniProtKB - P25519 (HFLX_ECOLI)
Protein
GTPase HflX
Gene
hflX
Organism
Escherichia coli (strain K12)
Status
Functioni
GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis. In vitro, also exhibits ATPase activity.UniRule annotation3 Publications
Cofactori
Mg2+UniRule annotation
Activity regulationi
Intrinsic GTPase activity is very slow and can be stimulated by the presence of 50S ribosomal subunits or 70S ribosomes. GTPase activity is inhibited by ATP.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 211 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 231 | MagnesiumUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 204 – 211 | GTPUniRule annotation | 8 | |
Nucleotide bindingi | 229 – 233 | GTPUniRule annotation | 5 | |
Nucleotide bindingi | 251 – 254 | GTPUniRule annotation | 4 | |
Nucleotide bindingi | 317 – 320 | GTPUniRule annotation | 4 | |
Nucleotide bindingi | 343 – 345 | GTPUniRule annotation | 3 |
GO - Molecular functioni
- ATPase activity Source: EcoCyc
- ATP binding Source: EcoliWiki
- GTPase activity Source: EcoCyc
- GTP binding Source: EcoCyc
- guanosine tetraphosphate binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- ribosomal large subunit binding Source: EcoCyc
- ribosome binding Source: EcoCyc
- rRNA binding Source: EcoCyc
GO - Biological processi
- peptidyl-serine autophosphorylation Source: EcoCyc
- rescue of stalled ribosome Source: EcoCyc
- response to heat Source: EcoCyc
- ribosome disassembly Source: EcoCyc
Keywordsi
Ligand | ATP-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10437-MONOMER MetaCyc:EG10437-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: GTPase HflXUniRule annotationAlternative name(s): GTP-binding protein HflXUniRule annotation |
Gene namesi | Name:hflXUniRule annotation Ordered Locus Names:b4173, JW4131 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Note: May associate with membranes.
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Disruption does not affect lambda lysogeny or the transposition frequency of transposable elements.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122463 | 1 – 426 | GTPase HflXAdd BLAST | 426 |
Proteomic databases
jPOSTi | P25519 |
PaxDbi | P25519 |
PRIDEi | P25519 |
Interactioni
Subunit structurei
Monomer. Associates with the 50S ribosomal subunit. This interaction occurs in the presence of GTP, GDP, ATP or ADP, but not in their absence.
UniRule annotation2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261252, 369 interactors 852980, 1 interactor |
DIPi | DIP-9895N |
IntActi | P25519, 1 interactor |
STRINGi | 511145.b4173 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 198 – 365 | Hflx-type GUniRule annotationAdd BLAST | 168 |
Domaini
Full-length protein is required for specific association with the 50S ribosomal subunit.1 Publication
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2262, Bacteria |
HOGENOMi | CLU_019597_2_1_6 |
InParanoidi | P25519 |
PhylomeDBi | P25519 |
Family and domain databases
CDDi | cd01878, HflX, 1 hit |
Gene3Di | 3.40.50.11060, 1 hit |
HAMAPi | MF_00900, GTPase_HflX, 1 hit |
InterProi | View protein in InterPro IPR035647, EFG_III/V IPR030394, G_HFLX_dom IPR006073, GTP-bd IPR032305, GTP-bd_M IPR016496, GTPase_HflX IPR025121, GTPase_HflX_N IPR042108, GTPase_HflX_N_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR10229, PTHR10229, 1 hit |
Pfami | View protein in Pfam PF16360, GTP-bdg_M, 1 hit PF13167, GTP-bdg_N, 1 hit PF01926, MMR_HSR1, 1 hit |
PIRSFi | PIRSF006809, GTP-binding_hflX_prd, 1 hit |
PRINTSi | PR00326, GTP1OBG |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 1 hit |
TIGRFAMsi | TIGR03156, GTP_HflX, 1 hit |
PROSITEi | View protein in PROSITE PS51705, G_HFLX, 1 hit |
i Sequence
Sequence statusi: Complete.
P25519-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFDRYDAGEQ AVLVHIYFTQ DKDMEDLQEF ESLVSSAGVE ALQVITGSRK
60 70 80 90 100
APHPKYFVGE GKAVEIAEAV KATGASVVLF DHALSPAQER NLERLCECRV
110 120 130 140 150
IDRTGLILDI FAQRARTHEG KLQVELAQLR HLATRLVRGW THLERQKGGI
160 170 180 190 200
GLRGPGETQL ETDRRLLRNR IVQIQSRLER VEKQREQGRQ SRIKADVPTV
210 220 230 240 250
SLVGYTNAGK STLFNRITEA RVYAADQLFA TLDPTLRRID VADVGETVLA
260 270 280 290 300
DTVGFIRHLP HDLVAAFKAT LQETRQATLL LHVIDAADVR VQENIEAVNT
310 320 330 340 350
VLEEIDAHEI PTLLVMNKID MLEDFEPRID RDEENKPNRV WLSAQTGAGI
360 370 380 390 400
PQLFQALTER LSGEVAQHTL RLPPQEGRLR SRFYQLQAIE KEWMEEDGSV
410 420
SLQVRMPIVD WRRLCKQEPA LIDYLI
Sequence cautioni
The sequence BAA00645 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 53 | H → R in BAA00645 (PubMed:2020545).Curated | 1 | |
Sequence conflicti | 56 | Y → S in BAA00645 (PubMed:2020545).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00005 Unassigned DNA Translation: AAC43398.1 U14003 Genomic DNA Translation: AAA97069.1 U00096 Genomic DNA Translation: AAC77130.1 AP009048 Genomic DNA Translation: BAE78174.1 D00743 Genomic DNA Translation: BAA00645.1 Frameshift. |
PIRi | S56398 |
RefSeqi | NP_418594.1, NC_000913.3 WP_000460362.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77130; AAC77130; b4173 BAE78174; BAE78174; BAE78174 |
GeneIDi | 948688 |
KEGGi | ecj:JW4131 eco:b4173 |
PATRICi | fig|1411691.4.peg.2528 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00005 Unassigned DNA Translation: AAC43398.1 U14003 Genomic DNA Translation: AAA97069.1 U00096 Genomic DNA Translation: AAC77130.1 AP009048 Genomic DNA Translation: BAE78174.1 D00743 Genomic DNA Translation: BAA00645.1 Frameshift. |
PIRi | S56398 |
RefSeqi | NP_418594.1, NC_000913.3 WP_000460362.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ADY | electron microscopy | 4.50 | 6 | 1-426 | [»] | |
5ZZM | electron microscopy | 8.10 | A | 1-426 | [»] | |
SMRi | P25519 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261252, 369 interactors 852980, 1 interactor |
DIPi | DIP-9895N |
IntActi | P25519, 1 interactor |
STRINGi | 511145.b4173 |
Proteomic databases
jPOSTi | P25519 |
PaxDbi | P25519 |
PRIDEi | P25519 |
Genome annotation databases
EnsemblBacteriai | AAC77130; AAC77130; b4173 BAE78174; BAE78174; BAE78174 |
GeneIDi | 948688 |
KEGGi | ecj:JW4131 eco:b4173 |
PATRICi | fig|1411691.4.peg.2528 |
Organism-specific databases
EchoBASEi | EB0432 |
Phylogenomic databases
eggNOGi | COG2262, Bacteria |
HOGENOMi | CLU_019597_2_1_6 |
InParanoidi | P25519 |
PhylomeDBi | P25519 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10437-MONOMER MetaCyc:EG10437-MONOMER |
Miscellaneous databases
PROi | PR:P25519 |
Family and domain databases
CDDi | cd01878, HflX, 1 hit |
Gene3Di | 3.40.50.11060, 1 hit |
HAMAPi | MF_00900, GTPase_HflX, 1 hit |
InterProi | View protein in InterPro IPR035647, EFG_III/V IPR030394, G_HFLX_dom IPR006073, GTP-bd IPR032305, GTP-bd_M IPR016496, GTPase_HflX IPR025121, GTPase_HflX_N IPR042108, GTPase_HflX_N_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR10229, PTHR10229, 1 hit |
Pfami | View protein in Pfam PF16360, GTP-bdg_M, 1 hit PF13167, GTP-bdg_N, 1 hit PF01926, MMR_HSR1, 1 hit |
PIRSFi | PIRSF006809, GTP-binding_hflX_prd, 1 hit |
PRINTSi | PR00326, GTP1OBG |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 1 hit |
TIGRFAMsi | TIGR03156, GTP_HflX, 1 hit |
PROSITEi | View protein in PROSITE PS51705, G_HFLX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HFLX_ECOLI | |
Accessioni | P25519Primary (citable) accession number: P25519 Secondary accession number(s): Q2M6D2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families