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Entry version 168 (12 Aug 2020)
Sequence version 1 (01 May 1992)
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Protein

Proline utilization trans-activator

Gene

PUT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Positive activator of the proline utilization pathway. Binds to the promoters of PUT1 and PUT2 genes. Recognizes and binds to the DNA sequence 5'-CGG-N(10)-CCG-3'.

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi34Zinc 11
Metal bindingi34Zinc 21
Metal bindingi37Zinc 11
Metal bindingi44Zinc 11
Metal bindingi50Zinc 11
Metal bindingi50Zinc 21
Metal bindingi53Zinc 21
Metal bindingi60Zinc 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi34 – 60Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST27

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding
Biological processProline metabolism, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-31824-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Proline utilization trans-activator
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:PUT3
Ordered Locus Names:YKL015W
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XI

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YKL015W

Saccharomyces Genome Database

More...
SGDi
S000001498, PUT3

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001149681 – 979Proline utilization trans-activatorAdd BLAST979

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P25502

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P25502

PRoteomics IDEntifications database

More...
PRIDEi
P25502

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P25502

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
34117, 104 interactors

Database of interacting proteins

More...
DIPi
DIP-1425N

Protein interaction database and analysis system

More...
IntActi
P25502, 5 interactors

Molecular INTeraction database

More...
MINTi
P25502

STRING: functional protein association networks

More...
STRINGi
4932.YKL015W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P25502, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1979
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P25502

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P25502

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG502QTA0, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000176304

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_006926_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P25502

KEGG Orthology (KO)

More...
KOi
K09248

Identification of Orthologs from Complete Genome Data

More...
OMAi
CTIASYF

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00067, GAL4, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
4.10.240.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR007219, Transcription_factor_dom_fun
IPR001138, Zn2-C6_fun-type_DNA-bd
IPR036864, Zn2-C6_fun-type_DNA-bd_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04082, Fungal_trans, 1 hit
PF00172, Zn_clus, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00906, Fungal_trans, 1 hit
SM00066, GAL4, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF57701, SSF57701, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00463, ZN2_CY6_FUNGAL_1, 1 hit
PS50048, ZN2_CY6_FUNGAL_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P25502-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVTDQGSRHS IQSKQPAYVN KQPQKRQQRS SVACLSCRKR HIKCPGGNPC
60 70 80 90 100
QKCVTSNAIC EYLEPSKKIV VSTKYLQQLQ KDLNDKTEEN NRLKALLLER
110 120 130 140 150
PVSVRGKDNS DDDERHINNA PSSDTLEVSS APAAPIFDLM SNSNTASDND
160 170 180 190 200
NDDDNSNRIT NNRSYDHSLE KYYKKAISIF KQPANANGEN GNGANGHEDD
210 220 230 240 250
DEDDEEISTN FAQRSGRLIE SHNGFHYFVG SSSMTLFGLE IQSLVTKYIS
260 270 280 290 300
VKNFRPLPIN TKNKILNSNL NPAISSFINS NNYLFSSYNF LNPISTIVNL
310 320 330 340 350
NSINDNLSPL MFKIILKSDT DGSSGQEEVI QFQLPSYNYT KLLIDCFINY
360 370 380 390 400
NDGCFYFFNE GLVKCGINKL YLENKWLYYD NTKKALDNEN DPILQAVWFC
410 420 430 440 450
KILLILAVGE MYLGSINNEM LKNYSNQPKL PGSKFFQMGS KIFNCLFSSE
460 470 480 490 500
RLENVTKKGG IEVLLLYAFF LQVADYTLAS YFYFGQALRT CLILGLHVDS
510 520 530 540 550
QSDTLSRYEI EHHRRLWWTV YMFERMLSSK AGLPLSFTDY TISTALPADI
560 570 580 590 600
DDETIEEKNS HYVFRKAELI SNCVTIVKIN AQILSKLYQR QPETNIIITL
610 620 630 640 650
KVVIKQLLEW RNNLSDSLQV DFTQKDEDFK ISRLSTNMFT EYFQGINLAV
660 670 680 690 700
RPLLFHFASI QLKRFKTSNT FVNLQNYSAT ISSLLTCSLH ASVNTIRSLW
710 720 730 740 750
SLLQNSMLAM FSYMDREYLF TSSCTLLLFN TAFGIHEQTL YHLDHSLEIF
760 770 780 790 800
TQMRNLGNIP AGLRRAQLLT LMANLDFHGI MNDLITKYND ILKFDSMNCE
810 820 830 840 850
NDNIVEDSNE PKRETEKCKP HKDGDRIDPS IIDCDKSNTN TNMIKNESIS
860 870 880 890 900
NIVSILPEGA KPTLTDYSNG NNDVNDINVN NSEPSTFFDI ITASLENSYQ
910 920 930 940 950
TTLTEKGSQV MEKNMDQLDS VHNLNDDDLQ QLLEDLGNID HSDEKLWKEI
960 970
TDQAMWLGNT MDPTAAAGSE IDFTDYLGP
Length:979
Mass (Da):111,414
Last modified:May 1, 1992 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i59FA19EFC79EE0C0
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X55384 Genomic DNA Translation: CAA39055.1
X74152 Genomic DNA Translation: CAA52267.1
Z28015 Genomic DNA Translation: CAA81850.1
BK006944 Genomic DNA Translation: DAA09141.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A39792

NCBI Reference Sequences

More...
RefSeqi
NP_012910.1, NM_001179581.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YKL015W_mRNA; YKL015W; YKL015W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
853854

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YKL015W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55384 Genomic DNA Translation: CAA39055.1
X74152 Genomic DNA Translation: CAA52267.1
Z28015 Genomic DNA Translation: CAA81850.1
BK006944 Genomic DNA Translation: DAA09141.1
PIRiA39792
RefSeqiNP_012910.1, NM_001179581.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJYNMR-A/B31-100[»]
1ZMEX-ray2.50C/D31-100[»]
SMRiP25502
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi34117, 104 interactors
DIPiDIP-1425N
IntActiP25502, 5 interactors
MINTiP25502
STRINGi4932.YKL015W

PTM databases

iPTMnetiP25502

Proteomic databases

MaxQBiP25502
PaxDbiP25502
PRIDEiP25502

Genome annotation databases

EnsemblFungiiYKL015W_mRNA; YKL015W; YKL015W
GeneIDi853854
KEGGisce:YKL015W

Organism-specific databases

EuPathDBiFungiDB:YKL015W
SGDiS000001498, PUT3

Phylogenomic databases

eggNOGiENOG502QTA0, Eukaryota
GeneTreeiENSGT00940000176304
HOGENOMiCLU_006926_0_0_1
InParanoidiP25502
KOiK09248
OMAiCTIASYF

Enzyme and pathway databases

BioCyciYEAST:G3O-31824-MONOMER

Miscellaneous databases

EvolutionaryTraceiP25502

Protein Ontology

More...
PROi
PR:P25502
RNActiP25502, protein

Family and domain databases

CDDicd00067, GAL4, 1 hit
Gene3Di4.10.240.10, 1 hit
InterProiView protein in InterPro
IPR007219, Transcription_factor_dom_fun
IPR001138, Zn2-C6_fun-type_DNA-bd
IPR036864, Zn2-C6_fun-type_DNA-bd_sf
PfamiView protein in Pfam
PF04082, Fungal_trans, 1 hit
PF00172, Zn_clus, 1 hit
SMARTiView protein in SMART
SM00906, Fungal_trans, 1 hit
SM00066, GAL4, 1 hit
SUPFAMiSSF57701, SSF57701, 1 hit
PROSITEiView protein in PROSITE
PS00463, ZN2_CY6_FUNGAL_1, 1 hit
PS50048, ZN2_CY6_FUNGAL_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPUT3_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P25502
Secondary accession number(s): D6VXS1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: August 12, 2020
This is version 168 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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