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Protein

DNA repair protein RAD51

Gene

RAD51

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required both for recombination and for the repair of DNA damage caused by X-rays. Its function may be modulated by interaction with other repair proteins. RAD52 interacts directly with RAD51, via its C-terminus. Forms a nucleoprotein filament with DNA as an early intermediate in recombination.1 Publication

Miscellaneous

Present with 6960 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 192ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: SGD
  • double-stranded DNA binding Source: GO_Central
  • identical protein binding Source: IntAct
  • recombinase activity Source: SGD
  • single-stranded DNA binding Source: SGD

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30262-MONOMER
ReactomeiR-SCE-5693616 Presynaptic phase of homologous DNA pairing and strand exchange

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51
Gene namesi
Name:RAD51
Ordered Locus Names:YER095W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

SGDiS000000897 RAD51

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229251 – 400DNA repair protein RAD51Add BLAST400

Proteomic databases

MaxQBiP25454
PaxDbiP25454
PRIDEiP25454

PTM databases

iPTMnetiP25454

Expressioni

Developmental stagei

RAD51 is cell cycle regulated, peaking around the G1-to-S transition.

Inductioni

By X-rays.

Interactioni

Subunit structurei

Part of a repair/recombination complex that includes RAD51, RAD52 and RAD54. Interacts with HED1, RDH54 and SAW1.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36841, 513 interactors
DIPiDIP-195N
IntActiP25454, 45 interactors
MINTiP25454
STRINGi4932.YER095W

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP25454
SMRiP25454
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25454

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00860000133731
HOGENOMiHOG000227426
InParanoidiP25454
KOiK04482
OMAiYNTDHQT
OrthoDBiEOG092C3DRU

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011941 DNA_recomb/repair_Rad51
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR010995 DNA_repair_Rad51/TF_NusA_a-hlx
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR020588 RecA_ATP-bd
IPR020587 RecA_monomer-monomer_interface
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF47794 SSF47794, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02239 recomb_RAD51, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit
PS50163 RECA_3, 1 hit

Sequencei

Sequence statusi: Complete.

P25454-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQVQEQHIS ESQLQYGNGS LMSTVPADLS QSVVDGNGNG SSEDIEATNG
60 70 80 90 100
SGDGGGLQEQ AEAQGEMEDE AYDEAALGSF VPIEKLQVNG ITMADVKKLR
110 120 130 140 150
ESGLHTAEAV AYAPRKDLLE IKGISEAKAD KLLNEAARLV PMGFVTAADF
160 170 180 190 200
HMRRSELICL TTGSKNLDTL LGGGVETGSI TELFGEFRTG KSQLCHTLAV
210 220 230 240 250
TCQIPLDIGG GEGKCLYIDT EGTFRPVRLV SIAQRFGLDP DDALNNVAYA
260 270 280 290 300
RAYNADHQLR LLDAAAQMMS ESRFSLIVVD SVMALYRTDF SGRGELSARQ
310 320 330 340 350
MHLAKFMRAL QRLADQFGVA VVVTNQVVAQ VDGGMAFNPD PKKPIGGNIM
360 370 380 390 400
AHSSTTRLGF KKGKGCQRLC KVVDSPCLPE AECVFAIYED GVGDPREEDE
Length:400
Mass (Da):42,963
Last modified:May 1, 1992 - v1
Checksum:i66561B60122A39A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64270 Genomic DNA Translation: CAA45563.1
M88470 Genomic DNA Translation: AAA34948.1
D10023 Genomic DNA Translation: BAA00913.1
U18839 Genomic DNA Translation: AAB64650.1
BK006939 Genomic DNA Translation: DAA07757.1
PIRiA44348
RefSeqiNP_011021.3, NM_001178986.3

Genome annotation databases

EnsemblFungiiYER095W; YER095W; YER095W
GeneIDi856831
KEGGisce:YER095W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64270 Genomic DNA Translation: CAA45563.1
M88470 Genomic DNA Translation: AAA34948.1
D10023 Genomic DNA Translation: BAA00913.1
U18839 Genomic DNA Translation: AAB64650.1
BK006939 Genomic DNA Translation: DAA07757.1
PIRiA44348
RefSeqiNP_011021.3, NM_001178986.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SZPX-ray3.25A/B/C/D/E/F81-400[»]
3LDAX-ray2.50A1-400[»]
ProteinModelPortaliP25454
SMRiP25454
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36841, 513 interactors
DIPiDIP-195N
IntActiP25454, 45 interactors
MINTiP25454
STRINGi4932.YER095W

PTM databases

iPTMnetiP25454

Proteomic databases

MaxQBiP25454
PaxDbiP25454
PRIDEiP25454

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER095W; YER095W; YER095W
GeneIDi856831
KEGGisce:YER095W

Organism-specific databases

SGDiS000000897 RAD51

Phylogenomic databases

GeneTreeiENSGT00860000133731
HOGENOMiHOG000227426
InParanoidiP25454
KOiK04482
OMAiYNTDHQT
OrthoDBiEOG092C3DRU

Enzyme and pathway databases

BioCyciYEAST:G3O-30262-MONOMER
ReactomeiR-SCE-5693616 Presynaptic phase of homologous DNA pairing and strand exchange

Miscellaneous databases

EvolutionaryTraceiP25454
PROiPR:P25454

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011941 DNA_recomb/repair_Rad51
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR010995 DNA_repair_Rad51/TF_NusA_a-hlx
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR020588 RecA_ATP-bd
IPR020587 RecA_monomer-monomer_interface
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF47794 SSF47794, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02239 recomb_RAD51, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit
PS50163 RECA_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD51_YEAST
AccessioniPrimary (citable) accession number: P25454
Secondary accession number(s): D3DM03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 7, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
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Main funding by: National Institutes of Health

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