Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tumor necrosis factor receptor superfamily member 6

Gene

Fas

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Receptor
Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-MMU-3371378 Regulation by c-FLIP
R-MMU-5213460 RIPK1-mediated regulated necrosis
R-MMU-5218900 CASP8 activity is inhibited
R-MMU-69416 Dimerization of procaspase-8
R-MMU-75157 FasL/ CD95L signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor necrosis factor receptor superfamily member 6
Alternative name(s):
Apo-1 antigen
Apoptosis-mediating surface antigen FAS
FASLG receptor
CD_antigen: CD95
Gene namesi
Name:Fas
Synonyms:Apt1, Tnfrsf6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:95484 Fas

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 169ExtracellularSequence analysisAdd BLAST148
Transmembranei170 – 186HelicalSequence analysisAdd BLAST17
Topological domaini187 – 327CytoplasmicSequence analysisAdd BLAST141

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Fas are the cause of the lymphoproliferation phenotype (lpr). Lpr mice show lymphadenopathy and autoantibody production.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000003456722 – 327Tumor necrosis factor receptor superfamily member 6Add BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi44 ↔ 55PROSITE-ProRule annotation
Disulfide bondi56 ↔ 69PROSITE-ProRule annotation
Disulfide bondi59 ↔ 78PROSITE-ProRule annotation
Disulfide bondi81 ↔ 97PROSITE-ProRule annotation
Disulfide bondi100 ↔ 115PROSITE-ProRule annotation
Disulfide bondi103 ↔ 123PROSITE-ProRule annotation
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi125 ↔ 139PROSITE-ProRule annotation
Disulfide bondi142 ↔ 153PROSITE-ProRule annotation
Disulfide bondi145 ↔ 161PROSITE-ProRule annotation
Modified residuei206PhosphothreonineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei314PhosphothreonineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP25446
PaxDbiP25446
PeptideAtlasiP25446
PRIDEiP25446

PTM databases

iPTMnetiP25446
PhosphoSitePlusiP25446
SwissPalmiP25446

Expressioni

Tissue specificityi

Detected in various tissues including thymus, liver, lung, heart, and adult ovary.1 Publication

Inductioni

Expression oscillates in a circadian manner in the liver with peak levels seen at CT12.1 Publication

Gene expression databases

BgeeiENSMUSG00000024778 Expressed in 185 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_FAS
ExpressionAtlasiP25446 baseline and differential
GenevisibleiP25446 MM

Interactioni

Subunit structurei

Binds DAXX (PubMed:9215629). Interacts with HIPK3 (PubMed:11034606). Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BABAM2 and FEM1B. Interacts with FADD (By similarity). Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (PubMed:15383280). Interacts with CALM (By similarity).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199594, 8 interactors
DIPiDIP-31093N
IntActiP25446, 11 interactors
MINTiP25446
STRINGi10090.ENSMUSP00000025691

Structurei

Secondary structure

1327
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP25446
SMRiP25446
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25446

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 79TNFR-Cys 1Add BLAST37
Repeati80 – 123TNFR-Cys 2Add BLAST44
Repeati124 – 162TNFR-Cys 3Add BLAST39
Domaini222 – 306DeathPROSITE-ProRule annotationAdd BLAST85

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni204 – 309Interaction with HIPK31 PublicationAdd BLAST106
Regioni222 – 246Interaction with CALMBy similarityAdd BLAST25

Domaini

Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J23A Eukaryota
ENOG4112ADB LUCA
GeneTreeiENSGT00730000111280
HOGENOMiHOG000139681
HOVERGENiHBG004091
InParanoidiP25446
KOiK04390
OMAiCTTCEHG
OrthoDBiEOG091G0DU6
TreeFamiTF333916

Family and domain databases

CDDicd08316 Death_FAS_TNFRSF6, 1 hit
cd10579 TNFRSF6, 1 hit
InterProiView protein in InterPro
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR008063 Fas_rcpt
IPR001368 TNFR/NGFR_Cys_rich_reg
IPR033998 TNFRSF6_death
IPR033999 TNFRSF6_N
PANTHERiPTHR23097:SF114 PTHR23097:SF114, 1 hit
PfamiView protein in Pfam
PF00531 Death, 1 hit
PF00020 TNFR_c6, 2 hits
PRINTSiPR01680 TNFACTORR6
SMARTiView protein in SMART
SM00005 DEATH, 1 hit
SM00208 TNFR, 3 hits
SUPFAMiSSF47986 SSF47986, 1 hit
PROSITEiView protein in PROSITE
PS50017 DEATH_DOMAIN, 1 hit
PS00652 TNFR_NGFR_1, 2 hits
PS50050 TNFR_NGFR_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P25446-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLWIWAVLPL VLAGSQLRVH TQGTNSISES LKLRRRVRET DKNCSEGLYQ
60 70 80 90 100
GGPFCCQPCQ PGKKKVEDCK MNGGTPTCAP CTEGKEYMDK NHYADKCRRC
110 120 130 140 150
TLCDEEHGLE VETNCTLTQN TKCKCKPDFY CDSPGCEHCV RCASCEHGTL
160 170 180 190 200
EPCTATSNTN CRKQSPRNRL WLLTILVLLI PLVFIYRKYR KRKCWKRRQD
210 220 230 240 250
DPESRTSSRE TIPMNASNLS LSKYIPRIAE DMTIQEAKKF ARENNIKEGK
260 270 280 290 300
IDEIMHDSIQ DTAEQKVQLL LCWYQSHGKS DAYQDLIKGL KKAECRRTLD
310 320
KFQDMVQKDL GKSTPDTGNE NEGQCLE
Length:327
Mass (Da):37,437
Last modified:July 27, 2011 - v2
Checksum:iD8DA95CA525CED56
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8C350Q8C350_MOUSE
Tumor necrosis factor receptor supe...
Fas
63Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38R → H in AAA37593 (PubMed:1371136).Curated1
Sequence conflicti38R → H in CAC00638 (Ref. 2) Curated1
Sequence conflicti38R → H in AAB25700 (PubMed:7680478).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti246I → N in lpr. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83649 mRNA Translation: AAA37593.1
AK002590 mRNA Translation: BAB22211.1
AJ295702, AJ295703, AJ295704 Genomic DNA Translation: CAC00638.1
DQ846748 mRNA Translation: ABI24112.1
CH466534 Genomic DNA Translation: EDL41748.1
BC061160 mRNA Translation: AAH61160.1
S56490, S56485, S56486 Genomic DNA Translation: AAB25700.1
CCDSiCCDS29758.1
PIRiA46484
RefSeqiNP_032013.2, NM_007987.2
UniGeneiMm.1626

Genome annotation databases

EnsembliENSMUST00000025691; ENSMUSP00000025691; ENSMUSG00000024778
GeneIDi14102
KEGGimmu:14102
UCSCiuc008hgi.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83649 mRNA Translation: AAA37593.1
AK002590 mRNA Translation: BAB22211.1
AJ295702, AJ295703, AJ295704 Genomic DNA Translation: CAC00638.1
DQ846748 mRNA Translation: ABI24112.1
CH466534 Genomic DNA Translation: EDL41748.1
BC061160 mRNA Translation: AAH61160.1
S56490, S56485, S56486 Genomic DNA Translation: AAB25700.1
CCDSiCCDS29758.1
PIRiA46484
RefSeqiNP_032013.2, NM_007987.2
UniGeneiMm.1626

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NA6NMR-A/B/C167-193[»]
3OQ9X-ray6.80A/B/C/D/E223-308[»]
ProteinModelPortaliP25446
SMRiP25446
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199594, 8 interactors
DIPiDIP-31093N
IntActiP25446, 11 interactors
MINTiP25446
STRINGi10090.ENSMUSP00000025691

PTM databases

iPTMnetiP25446
PhosphoSitePlusiP25446
SwissPalmiP25446

Proteomic databases

MaxQBiP25446
PaxDbiP25446
PeptideAtlasiP25446
PRIDEiP25446

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025691; ENSMUSP00000025691; ENSMUSG00000024778
GeneIDi14102
KEGGimmu:14102
UCSCiuc008hgi.2 mouse

Organism-specific databases

CTDi355
MGIiMGI:95484 Fas

Phylogenomic databases

eggNOGiENOG410J23A Eukaryota
ENOG4112ADB LUCA
GeneTreeiENSGT00730000111280
HOGENOMiHOG000139681
HOVERGENiHBG004091
InParanoidiP25446
KOiK04390
OMAiCTTCEHG
OrthoDBiEOG091G0DU6
TreeFamiTF333916

Enzyme and pathway databases

ReactomeiR-MMU-3371378 Regulation by c-FLIP
R-MMU-5213460 RIPK1-mediated regulated necrosis
R-MMU-5218900 CASP8 activity is inhibited
R-MMU-69416 Dimerization of procaspase-8
R-MMU-75157 FasL/ CD95L signaling

Miscellaneous databases

ChiTaRSiFas mouse
EvolutionaryTraceiP25446
PROiPR:P25446
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024778 Expressed in 185 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_FAS
ExpressionAtlasiP25446 baseline and differential
GenevisibleiP25446 MM

Family and domain databases

CDDicd08316 Death_FAS_TNFRSF6, 1 hit
cd10579 TNFRSF6, 1 hit
InterProiView protein in InterPro
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR008063 Fas_rcpt
IPR001368 TNFR/NGFR_Cys_rich_reg
IPR033998 TNFRSF6_death
IPR033999 TNFRSF6_N
PANTHERiPTHR23097:SF114 PTHR23097:SF114, 1 hit
PfamiView protein in Pfam
PF00531 Death, 1 hit
PF00020 TNFR_c6, 2 hits
PRINTSiPR01680 TNFACTORR6
SMARTiView protein in SMART
SM00005 DEATH, 1 hit
SM00208 TNFR, 3 hits
SUPFAMiSSF47986 SSF47986, 1 hit
PROSITEiView protein in PROSITE
PS50017 DEATH_DOMAIN, 1 hit
PS00652 TNFR_NGFR_1, 2 hits
PS50050 TNFR_NGFR_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTNR6_MOUSE
AccessioniPrimary (citable) accession number: P25446
Secondary accession number(s): Q6GT31, Q9DCQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 27, 2011
Last modified: October 10, 2018
This is version 191 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again