UniProtKB - P25437 (FRMA_ECOLI)
Protein
S-(hydroxymethyl)glutathione dehydrogenase
Gene
frmA
Organism
Escherichia coli (strain K12)
Status
Functioni
Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent. In addition, hemithiolacetals other than those formed from GSH, including omega-thiol fatty acids, also are substrates.1 Publication
Catalytic activityi
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 40 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 62 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 92 | Zinc 2By similarity | 1 | |
Metal bindingi | 95 | Zinc 2By similarity | 1 | |
Metal bindingi | 98 | Zinc 2By similarity | 1 | |
Metal bindingi | 106 | Zinc 2By similarity | 1 | |
Metal bindingi | 169 | Zinc 1; catalyticBy similarity | 1 |
GO - Molecular functioni
- alcohol dehydrogenase activity, zinc-dependent Source: EcoliWiki
- identical protein binding Source: IntAct
- S-(hydroxymethyl)glutathione dehydrogenase activity Source: EcoliWiki
- S-(hydroxymethyl)glutathione dehydrogenase NAD activity Source: UniProtKB-EC
- S-(hydroxymethyl)glutathione dehydrogenase NADP activity Source: UniProtKB-EC
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- ethanol oxidation Source: InterPro
- formaldehyde catabolic process Source: EcoliWiki
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:ADHC-MONOMER MetaCyc:ADHC-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:frmA Synonyms:adhC Ordered Locus Names:b0356, JW0347 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160775 | 1 – 369 | S-(hydroxymethyl)glutathione dehydrogenaseAdd BLAST | 369 |
Proteomic databases
jPOSTi | P25437 |
PaxDbi | P25437 |
PRIDEi | P25437 |
Expressioni
Inductioni
Induced by formaldehyde and repressed by FrmR.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P25437
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-556478,EBI-556478 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261803, 18 interactors 849385, 3 interactors |
DIPi | DIP-2901N |
IntActi | P25437, 8 interactors |
STRINGi | 511145.b0356 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1062, Bacteria |
HOGENOMi | CLU_026673_14_0_6 |
InParanoidi | P25437 |
PhylomeDBi | P25437 |
Family and domain databases
CDDi | cd08300, alcohol_DH_class_III, 1 hit |
InterProi | View protein in InterPro IPR014183, ADH_3 IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02818, adh_III_F_hyde, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
i Sequence
Sequence statusi: Complete.
P25437-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSRAAVAFA PGKPLEIVEI DVAPPKKGEV LIKVTHTGVC HTDAFTLSGD
60 70 80 90 100
DPEGVFPVVL GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECEFCRS
110 120 130 140 150
GKTNLCVAVR ETQGKGLMPD GTTRFSYNGQ PLYHYMGCST FSEYTVVAEV
160 170 180 190 200
SLAKINPEAN HEHVCLLGCG VTTGIGAVHN TAKVQPGDSV AVFGLGAIGL
210 220 230 240 250
AVVQGARQAK AGRIIAIDTN PKKFDLARRF GATDCINPND YDKPIKDVLL
260 270 280 290 300
DINKWGIDHT FECIGNVNVM RAALESAHRG WGQSVIIGVA VAGQEISTRP
310 320 330 340 350
FQLVTGRVWK GSAFGGVKGR SQLPGMVEDA MKGDIDLEPF VTHTMSLDEI
360
NDAFDLMHEG KSIRTVIRY
Sequence cautioni
The sequence AAB18081 differs from that shown. Reason: Frameshift.Curated
The sequence BAA12834 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | H → E AA sequence (PubMed:1731906).Curated | 1 | |
Sequence conflicti | 46 | T → G AA sequence (PubMed:1731906).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D85613 Genomic DNA Translation: BAA12834.1 Frameshift. D38504 Genomic DNA Translation: BAA22412.1 U73857 Genomic DNA Translation: AAB18081.1 Frameshift. U00096 Genomic DNA Translation: AAC73459.1 AP009048 Genomic DNA Translation: BAE76138.1 |
PIRi | D64763 |
RefSeqi | NP_414890.1, NC_000913.3 WP_000842106.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73459; AAC73459; b0356 BAE76138; BAE76138; BAE76138 |
GeneIDi | 944988 |
KEGGi | ecj:JW0347 eco:b0356 |
PATRICi | fig|1411691.4.peg.1922 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D85613 Genomic DNA Translation: BAA12834.1 Frameshift. D38504 Genomic DNA Translation: BAA22412.1 U73857 Genomic DNA Translation: AAB18081.1 Frameshift. U00096 Genomic DNA Translation: AAC73459.1 AP009048 Genomic DNA Translation: BAE76138.1 |
PIRi | D64763 |
RefSeqi | NP_414890.1, NC_000913.3 WP_000842106.1, NZ_LN832404.1 |
3D structure databases
SMRi | P25437 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261803, 18 interactors 849385, 3 interactors |
DIPi | DIP-2901N |
IntActi | P25437, 8 interactors |
STRINGi | 511145.b0356 |
Proteomic databases
jPOSTi | P25437 |
PaxDbi | P25437 |
PRIDEi | P25437 |
Genome annotation databases
EnsemblBacteriai | AAC73459; AAC73459; b0356 BAE76138; BAE76138; BAE76138 |
GeneIDi | 944988 |
KEGGi | ecj:JW0347 eco:b0356 |
PATRICi | fig|1411691.4.peg.1922 |
Organism-specific databases
EchoBASEi | EB4303 |
Phylogenomic databases
eggNOGi | COG1062, Bacteria |
HOGENOMi | CLU_026673_14_0_6 |
InParanoidi | P25437 |
PhylomeDBi | P25437 |
Enzyme and pathway databases
BioCyci | EcoCyc:ADHC-MONOMER MetaCyc:ADHC-MONOMER |
Miscellaneous databases
PROi | PR:P25437 |
Family and domain databases
CDDi | cd08300, alcohol_DH_class_III, 1 hit |
InterProi | View protein in InterPro IPR014183, ADH_3 IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02818, adh_III_F_hyde, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FRMA_ECOLI | |
Accessioni | P25437Primary (citable) accession number: P25437 Secondary accession number(s): P75696, Q2MC68, Q47533 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families