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Protein

S-(hydroxymethyl)glutathione dehydrogenase

Gene

frmA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent. In addition, hemithiolacetals other than those formed from GSH, including omega-thiol fatty acids, also are substrates.1 Publication

Catalytic activityi

S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Zinc 1; catalyticBy similarity1
Metal bindingi62Zinc 1; catalyticBy similarity1
Metal bindingi92Zinc 2By similarity1
Metal bindingi95Zinc 2By similarity1
Metal bindingi98Zinc 2By similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi169Zinc 1; catalyticBy similarity1

GO - Molecular functioni

  • alcohol dehydrogenase activity, zinc-dependent Source: EcoliWiki
  • identical protein binding Source: IntAct
  • S-(hydroxymethyl)glutathione dehydrogenase activity Source: EcoliWiki
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

  • ethanol oxidation Source: InterPro
  • formaldehyde catabolic process Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ADHC-MONOMER
MetaCyc:ADHC-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Alternative name(s):
Alcohol dehydrogenase class-3 (EC:1.1.1.1)
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
GSH-FDH
Gene namesi
Name:frmA
Synonyms:adhC
Ordered Locus Names:b0356, JW0347
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG50010 frmA

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001607751 – 369S-(hydroxymethyl)glutathione dehydrogenaseAdd BLAST369

Proteomic databases

EPDiP25437
PaxDbiP25437
PRIDEiP25437

Expressioni

Inductioni

Induced by formaldehyde and repressed by FrmR.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-556478,EBI-556478

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261803, 18 interactors
DIPiDIP-2901N
IntActiP25437, 8 interactors
STRINGi316385.ECDH10B_0311

Structurei

3D structure databases

ProteinModelPortaliP25437
SMRiP25437
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108I9Z Bacteria
COG1062 LUCA
HOGENOMiHOG000294674
InParanoidiP25437
KOiK00121
OMAiCNVCKHP
PhylomeDBiP25437

Family and domain databases

CDDicd08300 alcohol_DH_class_III, 1 hit
InterProiView protein in InterPro
IPR014183 ADH_3
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02818 adh_III_F_hyde, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

P25437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSRAAVAFA PGKPLEIVEI DVAPPKKGEV LIKVTHTGVC HTDAFTLSGD
60 70 80 90 100
DPEGVFPVVL GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECEFCRS
110 120 130 140 150
GKTNLCVAVR ETQGKGLMPD GTTRFSYNGQ PLYHYMGCST FSEYTVVAEV
160 170 180 190 200
SLAKINPEAN HEHVCLLGCG VTTGIGAVHN TAKVQPGDSV AVFGLGAIGL
210 220 230 240 250
AVVQGARQAK AGRIIAIDTN PKKFDLARRF GATDCINPND YDKPIKDVLL
260 270 280 290 300
DINKWGIDHT FECIGNVNVM RAALESAHRG WGQSVIIGVA VAGQEISTRP
310 320 330 340 350
FQLVTGRVWK GSAFGGVKGR SQLPGMVEDA MKGDIDLEPF VTHTMSLDEI
360
NDAFDLMHEG KSIRTVIRY
Length:369
Mass (Da):39,359
Last modified:November 1, 1997 - v3
Checksum:i35B59078F8173521
GO

Sequence cautioni

The sequence AAB18081 differs from that shown. Reason: Frameshift at position 26.Curated
The sequence BAA12834 differs from that shown. Reason: Frameshift at position 26.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41H → E AA sequence (PubMed:1731906).Curated1
Sequence conflicti46T → G AA sequence (PubMed:1731906).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA Translation: BAA12834.1 Frameshift.
D38504 Genomic DNA Translation: BAA22412.1
U73857 Genomic DNA Translation: AAB18081.1 Frameshift.
U00096 Genomic DNA Translation: AAC73459.1
AP009048 Genomic DNA Translation: BAE76138.1
PIRiD64763
RefSeqiNP_414890.1, NC_000913.3
WP_000842106.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73459; AAC73459; b0356
BAE76138; BAE76138; BAE76138
GeneIDi944988
KEGGiecj:JW0347
eco:b0356
PATRICifig|1411691.4.peg.1922

Similar proteinsi

Entry informationi

Entry nameiFRMA_ECOLI
AccessioniPrimary (citable) accession number: P25437
Secondary accession number(s): P75696, Q2MC68, Q47533
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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