UniProtKB - P25367 (RNQ1_YEAST)
Protein
[PIN+] prion protein RNQ1
Gene
RNQ1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Transferable epigenetic modifier which forms a prion responsible for the non-Mendelian trait [PIN+]. The native function of the soluble protein is unknown.2 Publications
Miscellaneous
[PIN+], also known as [RNQ+], is the prion form of RNQ1 (PubMed:10678178). [PIN+] is the result of a conformational change of the cellular RNQ1 protein that becomes self-propagating and infectious. This conformational change generates a form of RNQ1 that assembles into amyloid fibrils (PubMed:17097676). [PIN+] promotes de novo [PSI+] formation upon SUP35 overproduction (cross-seeding) (PubMed:11511345). [PIN+] can be cured by GdnHCl and by deletion of the molecular chaperone HSP104, which is required for [PIN+] propagation (PubMed:10678178).3 Publications
Present with 1140 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- identical protein binding Source: IntAct
Keywordsi
Molecular function | Prion |
Names & Taxonomyi
Protein namesi | Recommended name: [PIN+] prion protein RNQ1Alternative name(s): Rich in asparagine and glutamine protein 1 |
Gene namesi | Name:RNQ1 Ordered Locus Names:YCL028W ORF Names:YCL181, YCL28W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000533, RNQ1 |
VEuPathDBi | FungiDB:YCL028W |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097390 | 1 – 405 | [PIN+] prion protein RNQ1Add BLAST | 405 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 5 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 84 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 143 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P25367 |
PaxDbi | P25367 |
PRIDEi | P25367 |
PTM databases
iPTMneti | P25367 |
Interactioni
Binary interactionsi
Hide detailsP25367
With | #Exp. | IntAct |
---|---|---|
itself | 6 | EBI-21708,EBI-21708 |
SIS1 [P25294] | 2 | EBI-21708,EBI-17244 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 30955, 120 interactors |
DIPi | DIP-4323N |
IntActi | P25367, 38 interactors |
MINTi | P25367 |
STRINGi | 4932.YCL028W |
Miscellaneous databases
RNActi | P25367, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 153 – 402 | Prion domain (PrD)Add BLAST | 250 |
Domaini
The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.
Phylogenomic databases
eggNOGi | ENOG502S2FC, Eukaryota |
HOGENOMi | CLU_702391_0_0_1 |
InParanoidi | P25367 |
OMAi | SRKYYYV |
i Sequence
Sequence statusi: Complete.
P25367-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDTDKLISEA ESHFSQGNHA EAVAKLTSAA QSNPNDEQMS TIESLIQKIA
60 70 80 90 100
GYVMDNRSGG SDASQDRAAG GGSSFMNTLM ADSKGSSQTQ LGKLALLATV
110 120 130 140 150
MTHSSNKGSS NRGFDVGTVM SMLSGSGGGS QSMGASGLAA LASQFFKSGN
160 170 180 190 200
NSQGQGQGQG QGQGQGQGQG QGSFTALASL ASSFMNSNNN NQQGQNQSSG
210 220 230 240 250
GSSFGALASM ASSFMHSNNN QNSNNSQQGY NQSYQNGNQN SQGYNNQQYQ
260 270 280 290 300
GGNGGYQQQQ GQSGGAFSSL ASMAQSYLGG GQTQSNQQQY NQQGQNNQQQ
310 320 330 340 350
YQQQGQNYQH QQQGQQQQQG HSSSFSALAS MASSYLGNNS NSNSSYGGQQ
360 370 380 390 400
QANEYGRPQQ NGQQQSNEYG RPQYGGNQNS NGQHESFNFS GNFSQQNNNG
NQNRY
Sequence cautioni
The sequence AAA34615 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 181 | A → T in AAA34615 (PubMed:3302672).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 166 – 169 | Missing in strain: SCI7.2/b, SCI11.5/a and SCI11.5/c. | 4 | |
Natural varianti | 171 – 172 | Missing in strain: SCI7.2/a, SCI7.2/d, SCI11.5/b and SCI11.5/d. | 2 | |
Natural varianti | 289 – 299 | Missing in strain: SCI7.2/b, SCI7.2/d and SCI11.5/c. Add BLAST | 11 | |
Natural varianti | 293 – 303 | Missing in strain: SCI9. Add BLAST | 11 | |
Natural varianti | 360 | Q → H in strain: SCI3, SCI4, SCI7.2/a, SCI7.2/b, SCI7.2/d, SCI11.5/a, SCI11.5/b, SCI11.5/c and SCI11.5/d. | 1 | |
Natural varianti | 372 | P → PQHNGQQQSNEYGRP in strain: SCI7.2/b, SCI7.2/d and SCI11.5/c. | 1 | |
Natural varianti | 383 | Q → H in strain: SCI7.2/b,SCI7.2/d and SCI11.5/c. | 1 | |
Natural varianti | 387 | F → L in strain: SCI4. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16717 Genomic DNA Translation: AAA34615.1 Frameshift. X59720 Genomic DNA Translation: CAC42958.1 AY028674 Genomic DNA Translation: AAK26208.1 AY028675 Genomic DNA Translation: AAK26209.1 AY028676 Genomic DNA Translation: AAK26210.1 AY028677 Genomic DNA Translation: AAK26211.1 AY028678 Genomic DNA Translation: AAK26212.1 AY028679 Genomic DNA Translation: AAK26213.1 AY028680 Genomic DNA Translation: AAK26214.1 AY028681 Genomic DNA Translation: AAK26215.1 AY028682 Genomic DNA Translation: AAK26216.1 AY028683 Genomic DNA Translation: AAK26217.1 AY028684 Genomic DNA Translation: AAK26218.1 AY028685 Genomic DNA Translation: AAK26219.1 BK006937 Genomic DNA Translation: DAA07456.1 |
PIRi | S19355 |
RefSeqi | NP_009902.2, NM_001178673.1 |
Genome annotation databases
EnsemblFungii | YCL028W_mRNA; YCL028W; YCL028W |
GeneIDi | 850329 |
KEGGi | sce:YCL028W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16717 Genomic DNA Translation: AAA34615.1 Frameshift. X59720 Genomic DNA Translation: CAC42958.1 AY028674 Genomic DNA Translation: AAK26208.1 AY028675 Genomic DNA Translation: AAK26209.1 AY028676 Genomic DNA Translation: AAK26210.1 AY028677 Genomic DNA Translation: AAK26211.1 AY028678 Genomic DNA Translation: AAK26212.1 AY028679 Genomic DNA Translation: AAK26213.1 AY028680 Genomic DNA Translation: AAK26214.1 AY028681 Genomic DNA Translation: AAK26215.1 AY028682 Genomic DNA Translation: AAK26216.1 AY028683 Genomic DNA Translation: AAK26217.1 AY028684 Genomic DNA Translation: AAK26218.1 AY028685 Genomic DNA Translation: AAK26219.1 BK006937 Genomic DNA Translation: DAA07456.1 |
PIRi | S19355 |
RefSeqi | NP_009902.2, NM_001178673.1 |
3D structure databases
SMRi | P25367 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 30955, 120 interactors |
DIPi | DIP-4323N |
IntActi | P25367, 38 interactors |
MINTi | P25367 |
STRINGi | 4932.YCL028W |
PTM databases
iPTMneti | P25367 |
Proteomic databases
MaxQBi | P25367 |
PaxDbi | P25367 |
PRIDEi | P25367 |
Genome annotation databases
EnsemblFungii | YCL028W_mRNA; YCL028W; YCL028W |
GeneIDi | 850329 |
KEGGi | sce:YCL028W |
Organism-specific databases
SGDi | S000000533, RNQ1 |
VEuPathDBi | FungiDB:YCL028W |
Phylogenomic databases
eggNOGi | ENOG502S2FC, Eukaryota |
HOGENOMi | CLU_702391_0_0_1 |
InParanoidi | P25367 |
OMAi | SRKYYYV |
Miscellaneous databases
PROi | PR:P25367 |
RNActi | P25367, protein |
Family and domain databases
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNQ1_YEAST | |
Accessioni | P25367Primary (citable) accession number: P25367 Secondary accession number(s): D6VQY7 Q8TFP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | April 26, 2004 | |
Last modified: | February 10, 2021 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names