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Protein

Exosome complex component RRP43

Gene

RRP43

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP43 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation.4 Publications

Miscellaneous

Present with 3180 molecules/cell in log phase SD medium.1 Publication

Caution

According to PubMed:17173052 and PubMed:17174896, only DIS3/RRP44 subunit of the exosome core has exonuclease activity.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processrRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29348-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Exosome complex component RRP43
Alternative name(s):
Ribosomal RNA-processing protein 43
Gene namesi
Name:RRP43
Ordered Locus Names:YCR035C
ORF Names:YCR35C, YCR522
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR035C
SGDiS000000631 RRP43

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Exosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi162S → F: Interaction with RRP46 abolished; when associated with T-246. 1 Publication1
Mutagenesisi212V → A: Interaction with RRP46 abolished. 1 Publication1
Mutagenesisi230C → Y: Interaction with RRP46 abolished; when associated with T-274 and Y-276. 1 Publication1
Mutagenesisi246A → T: Interaction with RRP46 abolished; when associated with F-162. 1 Publication1
Mutagenesisi274I → T: Interaction with RRP46 abolished; when associated with Y-230 and Y-276. 1 Publication1
Mutagenesisi276C → Y: Interaction with RRP46 abolished; when associated with Y-230 and T-274. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001399702 – 394Exosome complex component RRP43Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei26PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP25359
PaxDbiP25359
PRIDEiP25359

PTM databases

iPTMnetiP25359

Interactioni

Subunit structurei

Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which associates with catalytic subunits DIS3 and RRP6 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits and peripheral S1 domain-containing components CSL4, RRP4 and RRP40 located on the top of the ring structure. Interacts with NIP7 and NOP8. Interacts strongly with RRP46.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi31018, 134 interactors
ComplexPortaliCPX-599 Nuclear/nucleolar exosome complex, DIS3-RRP6 variant
CPX-603 Cytoplasmic exosome complex, DIS3 variant
DIPiDIP-5485N
IntActiP25359, 18 interactors
MINTiP25359
STRINGi4932.YCR035C

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP25359
SMRiP25359
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.Curated

Phylogenomic databases

HOGENOMiHOG000057141
InParanoidiP25359
KOiK12586
OMAiYVLYAKI
OrthoDBiEOG092C5179

Family and domain databases

Gene3Di3.30.230.70, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR033196 Rrp43
PANTHERiPTHR11097:SF9 PTHR11097:SF9, 1 hit
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
SUPFAMiSSF54211 SSF54211, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25359-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAESTTLETI EIHPITFPPE VLARISPELS LQRHLSLGIR PCLRKYEEFR
60 70 80 90 100
DVAIENNTLS RYADAGNIDT KNNILGSNVL KSGKTIVITS ITGGIIEETS
110 120 130 140 150
AAIKDLDDFG EEELFEVTKE EDIIANYASV YPVVEVERGR VGACTDEEMT
160 170 180 190 200
ISQKLHDSIL HSRILPKKAL KVKAGVRSAN EDGTFSVLYP DELEDDTLNE
210 220 230 240 250
TNLKMKRKWS YVLYAKIVVL SRTGPVFDLC WNSLMYALQS VKLPRAFIDE
260 270 280 290 300
RASDLRMTIR TRGRSATIRE TYEIICDQTK SVPLMINAKN IAFASNYGIV
310 320 330 340 350
ELDPECQLQN SDNSEEEEVD IDMDKLNTVL IADLDTEAEE TSIHSTISIL
360 370 380 390
AAPSGNYKQL TLVGGGAKIT PEMIKRSLLL SRVRADDLST RFNI
Length:394
Mass (Da):44,011
Last modified:June 27, 2003 - v2
Checksum:i8801837B8184134D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102A → S in CAA40227 (PubMed:1964349).Curated1
Sequence conflicti363V → M in CAA40227 (PubMed:1964349).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56909 Genomic DNA Translation: CAA40227.1
X59720 Genomic DNA Translation: CAC42984.1
S78624 Genomic DNA Translation: AAB21261.1
BK006937 Genomic DNA Translation: DAA07514.1
PIRiS12917
RefSeqiNP_009964.2, NM_001178749.1

Genome annotation databases

EnsemblFungiiCAC42984; CAC42984; CAC42984
YCR035C; YCR035C; YCR035C
GeneIDi850401
KEGGisce:YCR035C

Similar proteinsi

Entry informationi

Entry nameiRRP43_YEAST
AccessioniPrimary (citable) accession number: P25359
Secondary accession number(s): D6VR45, Q8NKJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 27, 2003
Last modified: September 12, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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