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Entry version 186 (02 Jun 2021)
Sequence version 2 (01 Oct 1996)
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Protein

Pumilio homology domain family member 4

Gene

PUF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Is not essential for haploid growth, but may affect diploid formation.

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • mRNA binding Source: SGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processTranscription, Transcription regulation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Pumilio homology domain family member 4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:PUF4
Ordered Locus Names:YGL014W
ORF Names:YGL023
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome VII

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000002982, PUF4

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YGL014W

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000759251 – 888Pumilio homology domain family member 4Add BLAST888

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei205PhosphothreonineCombined sources1
Modified residuei212PhosphothreonineCombined sources1
Modified residuei252PhosphothreonineCombined sources1
Modified residuei256PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P25339

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P25339

PRoteomics IDEntifications database

More...
PRIDEi
P25339

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P25339

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
33232, 686 interactors

Database of interacting proteins

More...
DIPi
DIP-2638N

Protein interaction database and analysis system

More...
IntActi
P25339, 18 interactors

Molecular INTeraction database

More...
MINTi
P25339

STRING: functional protein association networks

More...
STRINGi
4932.YGL014W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P25339, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1888
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P25339

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P25339

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini539 – 888PUM-HDPROSITE-ProRule annotationAdd BLAST350
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati563 – 598Pumilio 1Add BLAST36
Repeati599 – 634Pumilio 2Add BLAST36
Repeati635 – 671Pumilio 3Add BLAST37
Repeati672 – 707Pumilio 4Add BLAST36
Repeati708 – 743Pumilio 5Add BLAST36
Repeati744 – 783Pumilio 6Add BLAST40
Repeati784 – 821Pumilio 7Add BLAST38
Repeati823 – 861Pumilio 8Add BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni236 – 270DisorderedSequence analysisAdd BLAST35
Regioni467 – 551DisorderedSequence analysisAdd BLAST85

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi467 – 544Polar residuesSequence analysisAdd BLAST78

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2049, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_016143_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P25339

Identification of Orthologs from Complete Genome Data

More...
OMAi
LMTDSFG

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07920, Pumilio, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.25.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR033133, PUM-HD
IPR033712, Pumilio_RNA-bd
IPR001313, Pumilio_RNA-bd_rpt

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00806, PUF, 8 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00025, Pumilio, 8 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48371, SSF48371, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50302, PUM, 8 hits
PS50303, PUM_HD, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P25339-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTKGLKEEI DDVPSVDPVV SETVNSALEQ LQLDDPEENA TSNAFANKVS
60 70 80 90 100
QDSQFANGPP SQMFPHPQMM GGMGFMPYSQ MMQVPHNPCP FFPPPDFNDP
110 120 130 140 150
TAPLSSSPLN AGGPPMLFKN DSLPFQMLSS GAAVATQGGQ NLNPLINDNS
160 170 180 190 200
MKVLPIASAD PLWTHSNVPG SASVAIEETT ATLQESLPSK GRESNNKASS
210 220 230 240 250
FRRQTFHALS PTDLINAANN VTLSKDFQSD MQNFSKAKKP SVGANNTAKT
260 270 280 290 300
RTQSISFDNT PSSTSFIPPT NSVSEKLSDF KIETSKEDLI NKTAPAKKES
310 320 330 340 350
PTTYGAAYPY GGPLLQPNPI MPGHPHNISS PIYGIRSPFP NSYEMGAQFQ
360 370 380 390 400
PFSPILNPTS HSLNANSPIP LTQSPIHLAP VLNPSSNSVA FSDMKNDGGK
410 420 430 440 450
PTTDNDKAGP NVRMDLINPN LGPSMQPFHI LPPQQNTPPP PWLYSTPPPF
460 470 480 490 500
NAMVPPHLLA QNHMPLMNSA NNKHHGRNNN SMSSHNDNDN IGNSNYNNKD
510 520 530 540 550
TGRSNVGKMK NMKNSYHGYY NNNNNNNNNN NNNNNSNATN SNSAEKQRKI
560 570 580 590 600
EESSRFADAV LDQYIGSIHS LCKDQHGCRF LQKQLDILGS KAADAIFEET
610 620 630 640 650
KDYTVELMTD SFGNYLIQKL LEEVTTEQRI VLTKISSPHF VEISLNPHGT
660 670 680 690 700
RALQKLIECI KTDEEAQIVV DSLRPYTVQL SKDLNGNHVI QKCLQRLKPE
710 720 730 740 750
NFQFIFDAIS DSCIDIATHR HGCCVLQRCL DHGTTEQCDN LCDKLLALVD
760 770 780 790 800
KLTLDPFGNY VVQYIITKEA EKNKYDYTHK IVHLLKPRAI ELSIHKFGSN
810 820 830 840 850
VIEKILKTAI VSEPMILEIL NNGGETGIQS LLNDSYGNYV LQTALDISHK
860 870 880
QNDYLYKRLS EIVAPLLVGP IRNTPHGKRI IGMLHLDS
Length:888
Mass (Da):97,798
Last modified:October 1, 1996 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i659BA1062439F642
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti595A → R (PubMed:1909073).Curated1
Sequence conflicti595A → R (PubMed:1882553).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
S57889 Genomic DNA Translation: AAB19616.1
S58126 Genomic DNA Translation: AAD13898.1
Z72536 Genomic DNA Translation: CAA96714.1
BK006941 Genomic DNA Translation: DAA08084.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S64016

NCBI Reference Sequences

More...
RefSeqi
NP_011501.1, NM_001180879.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YGL014W_mRNA; YGL014W; YGL014W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
852870

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YGL014W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57889 Genomic DNA Translation: AAB19616.1
S58126 Genomic DNA Translation: AAD13898.1
Z72536 Genomic DNA Translation: CAA96714.1
BK006941 Genomic DNA Translation: DAA08084.1
PIRiS64016
RefSeqiNP_011501.1, NM_001180879.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BWTX-ray2.69A554-886[»]
3BX2X-ray2.84A/B554-888[»]
3BX3X-ray3.00A/B554-888[»]
4DZSX-ray3.14A/B536-888[»]
SMRiP25339
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi33232, 686 interactors
DIPiDIP-2638N
IntActiP25339, 18 interactors
MINTiP25339
STRINGi4932.YGL014W

PTM databases

iPTMnetiP25339

Proteomic databases

MaxQBiP25339
PaxDbiP25339
PRIDEiP25339

Genome annotation databases

EnsemblFungiiYGL014W_mRNA; YGL014W; YGL014W
GeneIDi852870
KEGGisce:YGL014W

Organism-specific databases

SGDiS000002982, PUF4
VEuPathDBiFungiDB:YGL014W

Phylogenomic databases

eggNOGiKOG2049, Eukaryota
HOGENOMiCLU_016143_0_0_1
InParanoidiP25339
OMAiLMTDSFG

Miscellaneous databases

EvolutionaryTraceiP25339

Protein Ontology

More...
PROi
PR:P25339
RNActiP25339, protein

Family and domain databases

CDDicd07920, Pumilio, 1 hit
Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR033133, PUM-HD
IPR033712, Pumilio_RNA-bd
IPR001313, Pumilio_RNA-bd_rpt
PfamiView protein in Pfam
PF00806, PUF, 8 hits
SMARTiView protein in SMART
SM00025, Pumilio, 8 hits
SUPFAMiSSF48371, SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50302, PUM, 8 hits
PS50303, PUM_HD, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPUF4_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P25339
Secondary accession number(s): D6VUC3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 1, 1996
Last modified: June 2, 2021
This is version 186 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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