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UniProtKB - P25337 (BUD31_YEAST)
Protein
Pre-mRNA-splicing factor BUD31
Gene
BUD31
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in pre-mRNA splicing. Important for bud site selection.
Miscellaneous
Present with 1900 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- cellular bud site selection Source: SGD
- mRNA splicing, via spliceosome Source: SGD
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor BUD31Alternative name(s): Bud site selection protein 31 Complexed with CEF1 protein 14 |
Gene namesi | Name:BUD31 Synonyms:CWC14 Ordered Locus Names:YCR063W ORF Names:YCR63W, YCR903 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000659, BUD31 |
VEuPathDBi | FungiDB:YCR063W |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: SGD
- spliceosomal complex Source: GO_Central
- U2 snRNP Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193902 | 1 – 157 | Pre-mRNA-splicing factor BUD31Add BLAST | 157 |
Proteomic databases
MaxQBi | P25337 |
PaxDbi | P25337 |
PRIDEi | P25337 |
Interactioni
Subunit structurei
Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
1 PublicationBinary interactionsi
P25337
With | #Exp. | IntAct |
---|---|---|
CEF1 [Q03654] | 12 | EBI-547,EBI-476 |
Protein-protein interaction databases
BioGRIDi | 31040, 742 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-1180N |
IntActi | P25337, 44 interactors |
MINTi | P25337 |
STRINGi | 4932.YCR063W |
Miscellaneous databases
RNActi | P25337, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P25337 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 2 – 11 | Nuclear localization signalSequence analysis | 10 |
Sequence similaritiesi
Belongs to the BUD31 (G10) family.Curated
Phylogenomic databases
eggNOGi | KOG3404, Eukaryota |
GeneTreei | ENSGT00390000014300 |
HOGENOMi | CLU_087132_1_1_1 |
InParanoidi | P25337 |
OMAi | CIQSRDM |
Family and domain databases
InterProi | View protein in InterPro IPR018230, BUD31/G10-rel_CS IPR001748, G10 |
PANTHERi | PTHR19411, PTHR19411, 1 hit |
Pfami | View protein in Pfam PF01125, G10, 1 hit |
PRINTSi | PR00322, G10 |
PROSITEi | View protein in PROSITE PS00997, G10_1, 1 hit PS00998, G10_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P25337-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW
60 70 80 90 100
EIMQLHHQRS RYIYTLYYKR KAISKDLYDW LIKEKYADKL LIAKWRKTGY
110 120 130 140 150
EKLCCLRCIQ KNETNNGSTC ICRVPRAQLE EEARKKGTQV SFHQCVHCGC
RGCASTD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59720 Genomic DNA Translation: CAA42278.1 BK006937 Genomic DNA Translation: DAA07536.1 |
PIRi | S22261 |
RefSeqi | NP_009990.1, NM_001178774.1 |
Genome annotation databases
EnsemblFungii | YCR063W_mRNA; YCR063W; YCR063W |
GeneIDi | 850427 |
KEGGi | sce:YCR063W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59720 Genomic DNA Translation: CAA42278.1 BK006937 Genomic DNA Translation: DAA07536.1 |
PIRi | S22261 |
RefSeqi | NP_009990.1, NM_001178774.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MY1 | NMR | - | A | 2-157 | [»] | |
5GM6 | electron microscopy | 3.50 | T | 1-157 | [»] | |
5GMK | electron microscopy | 3.40 | T | 1-157 | [»] | |
5LJ3 | electron microscopy | 3.80 | L | 1-157 | [»] | |
5LJ5 | electron microscopy | 3.80 | L | 1-157 | [»] | |
5LQW | electron microscopy | 5.80 | E | 1-157 | [»] | |
5MPS | electron microscopy | 3.85 | L | 1-157 | [»] | |
5MQ0 | electron microscopy | 4.17 | L | 1-157 | [»] | |
5WSG | electron microscopy | 4.00 | T | 1-157 | [»] | |
5Y88 | electron microscopy | 3.70 | L | 1-157 | [»] | |
5YLZ | electron microscopy | 3.60 | L | 1-157 | [»] | |
6BK8 | electron microscopy | 3.30 | I | 1-157 | [»] | |
6EXN | electron microscopy | 3.70 | L | 1-157 | [»] | |
6J6G | electron microscopy | 3.20 | T | 1-157 | [»] | |
6J6H | electron microscopy | 3.60 | T | 1-157 | [»] | |
6J6N | electron microscopy | 3.86 | T | 1-157 | [»] | |
6J6Q | electron microscopy | 3.70 | T | 1-157 | [»] | |
SMRi | P25337 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31040, 742 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-1180N |
IntActi | P25337, 44 interactors |
MINTi | P25337 |
STRINGi | 4932.YCR063W |
Proteomic databases
MaxQBi | P25337 |
PaxDbi | P25337 |
PRIDEi | P25337 |
Genome annotation databases
EnsemblFungii | YCR063W_mRNA; YCR063W; YCR063W |
GeneIDi | 850427 |
KEGGi | sce:YCR063W |
Organism-specific databases
SGDi | S000000659, BUD31 |
VEuPathDBi | FungiDB:YCR063W |
Phylogenomic databases
eggNOGi | KOG3404, Eukaryota |
GeneTreei | ENSGT00390000014300 |
HOGENOMi | CLU_087132_1_1_1 |
InParanoidi | P25337 |
OMAi | CIQSRDM |
Miscellaneous databases
PROi | PR:P25337 |
RNActi | P25337, protein |
Family and domain databases
InterProi | View protein in InterPro IPR018230, BUD31/G10-rel_CS IPR001748, G10 |
PANTHERi | PTHR19411, PTHR19411, 1 hit |
Pfami | View protein in Pfam PF01125, G10, 1 hit |
PRINTSi | PR00322, G10 |
PROSITEi | View protein in PROSITE PS00997, G10_1, 1 hit PS00998, G10_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BUD31_YEAST | |
Accessioni | P25337Primary (citable) accession number: P25337 Secondary accession number(s): D6VR67 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families